Rorug02G0459700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010587
Physical Location & Seq
Reverse (-)
59156390 .. 59157276
887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0459700.1

Sequence Viewer

Length: 546 bp
ATGAAGAGACCATCTCTGCTGTGTCAACCAGAGCTACAAAAAAATGCCGCACTGGCGATGGATCAGGCAAGTCGAGGTGAGGAAGCGATTTCAAGAGATCAGAGGCTCAAACCCGATCATCCCACTTTTGTTGTCATGATGCAAGAACCCTCCTACCTTACAACTGCCTTGAAACTTGATATAAACTTTGCCCAAAAATATGATTTATCGAGACGCAATGTTAACCTACAGATTCCAAATGGGAGCACTTGGCCTGTTATTTGCTCTGTCACCAGCGAAGACGGAATCCGCTACACCAGCGAAGGAGTTGCAAGACACCGCTATCCCAGTTGGTGTATCAGAGAAGAAGTTGCAAGAATCCGCTATCGTGGTTGGATAAAATTTGCAACAGACAATGGGCTGCAAGTAGGGGATACTTGTGTGTTTGAGATGATAAAGCAGCAGCCTGAAATAACGTTCTTAGTTCACATAATCCGAGCTACTTACGCTACTAAGCTCCATTTCAACAATATTTCCCAGAACATGAAGTTACGAAGAATTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.94

Weight (kDa)

9.41

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 43 - 160 4.2e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 48, 289, 319, 361
AclI AACGTT 1 cut(s) 455
AclWI GGATC 1 cut(s) 69
AcsI RAATTY 2 cut(s) 380, 537
AgsI TTSAA 3 cut(s) 93, 172, 505
AluBI AGCT 3 cut(s) 34, 479, 496
AluI AGCT 3 cut(s) 34, 479, 496
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 1 cut(s) 69
AoxI GGCC 1 cut(s) 251
ApeKI GCWGC 3 cut(s) 400, 439, 442
ApoI RAATTY 2 cut(s) 380, 537
AsuHPI GGTGA 2 cut(s) 89, 262
BaeI ACNNNNGTAYC 2 cut(s) 405, 438
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 248
BbvI GCAGC 3 cut(s) 387, 451, 454
BccI CCATC 2 cut(s) 19, 52
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BciVI GTATCC 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 205
BfmI CTRYAG 1 cut(s) 227
BfuI GTATCC 1 cut(s) 406
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 4 cut(s) 48, 401, 440, 443
BlsI GCNGC 4 cut(s) 49, 402, 441, 444
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 129
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 285
BplI GAGNNNNNCTC 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 57, 327
Bse3DI GCAATG 1 cut(s) 223
BseGI GGATG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 223
BseNI ACTGG 2 cut(s) 57, 327
BseXI GCAGC 3 cut(s) 387, 451, 454
BshFI GGCC 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 248
BsmAI GTCTC 1 cut(s) 205
BsmBI CGTCTC 1 cut(s) 205
BsnI GGCC 1 cut(s) 253
Bsp1286I GDGCHC 1 cut(s) 248
Bsp143I GATC 3 cut(s) 61, 97, 115
BspACI CCGC 4 cut(s) 48, 289, 319, 361
BspANI GGCC 1 cut(s) 253
BspHI TCATGA 1 cut(s) 135
BspPI GGATC 1 cut(s) 69
BsrDI GCAATG 1 cut(s) 223
BsrI ACTGG 2 cut(s) 57, 327
BssMI GATC 3 cut(s) 61, 97, 115
BstDEI CTNAG 2 cut(s) 460, 492
BstF5I GGATG 1 cut(s) 118
BstKTI GATC 3 cut(s) 64, 100, 118
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 3 cut(s) 61, 97, 115
BstMWI GCNNNNNNNGC 3 cut(s) 53, 297, 485
BstSFI CTRYAG 1 cut(s) 227
BstV1I GCAGC 3 cut(s) 387, 451, 454
BstV2I GAAGAC 1 cut(s) 285
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 1 cut(s) 253
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 50
CciI TCATGA 1 cut(s) 135
CseI GACGC 1 cut(s) 222
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
CviAII CATG 2 cut(s) 136, 523
CviJI RGCY 7 cut(s) 34, 106, 253, 400, 445, 479, 496
CviKI_1 RGCY 7 cut(s) 34, 106, 253, 400, 445, 479, 496
DdeI CTNAG 2 cut(s) 460, 492
DpnI GATC 3 cut(s) 63, 99, 117
DpnII GATC 3 cut(s) 61, 97, 115
EcoRI GAATTC 1 cut(s) 537
Esp3I CGTCTC 1 cut(s) 205
FaeI CATG 2 cut(s) 139, 526
FaiI YATR 5 cut(s) 137, 182, 201, 470, 524
FatI CATG 2 cut(s) 135, 522
Fnu4HI GCNGC 4 cut(s) 48, 401, 440, 443
FokI GGATG 1 cut(s) 105
Fsp4HI GCNGC 4 cut(s) 48, 401, 440, 443
GluI GCNGC 4 cut(s) 48, 401, 440, 443
HaeIII GGCC 1 cut(s) 253
HgaI GACGC 1 cut(s) 222
Hin1II CATG 2 cut(s) 139, 526
HincII GTYRAC 2 cut(s) 26, 223
HindII GTYRAC 2 cut(s) 26, 223
HinfI GANTC 3 cut(s) 232, 285, 357
HpaI GTTAAC 1 cut(s) 223
HphI GGTGA 2 cut(s) 89, 262
Hpy166II GTNNAC 3 cut(s) 26, 223, 466
Hpy188I TCNGA 3 cut(s) 102, 341, 476
Hpy188III TCNNGA 3 cut(s) 93, 136, 210
Hpy8I GTNNAC 3 cut(s) 26, 223, 466
HpyAV CCTTC 1 cut(s) 296
HpyCH4IV ACGT 1 cut(s) 455
HpyCH4V TGCA 5 cut(s) 142, 311, 353, 386, 403
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 297, 485
HpyF3I CTNAG 2 cut(s) 460, 492
HpySE526I ACGT 1 cut(s) 455
Hsp92II CATG 2 cut(s) 139, 526
KspAI GTTAAC 1 cut(s) 223
Kzo9I GATC 3 cut(s) 61, 97, 115
LmnI GCTCC 2 cut(s) 243, 501
LpnPI CCDG 9 cut(s) 38, 42, 50, 267, 286, 310, 340, 459, 530
Lsp1109I GCAGC 3 cut(s) 387, 451, 454
LweI GCATC 1 cut(s) 129
MaeII ACGT 1 cut(s) 455
MaeIII GTNAC 2 cut(s) 268, 528
MalI GATC 3 cut(s) 63, 99, 117
MboI GATC 3 cut(s) 61, 97, 115
MboII GAAGA 4 cut(s) 16, 290, 356, 546
MhlI GDGCHC 1 cut(s) 248
MluCI AATT 2 cut(s) 380, 537
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 4 cut(s) 68, 73, 96, 160
MseI TTAA 1 cut(s) 222
MwoI GCNNNNNNNGC 3 cut(s) 53, 297, 485
NdeII GATC 3 cut(s) 61, 97, 115
NlaIII CATG 2 cut(s) 139, 526
NmuCI GTSAC 1 cut(s) 268
PagI TCATGA 1 cut(s) 135
PfeI GAWTC 3 cut(s) 232, 285, 357
PkrI GCNGC 4 cut(s) 49, 402, 441, 444
Psp1406I AACGTT 1 cut(s) 455
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 4 cut(s) 48, 401, 440, 443
Sau3AI GATC 3 cut(s) 61, 97, 115
SduI GDGCHC 1 cut(s) 248
SetI ASST 7 cut(s) 36, 79, 159, 228, 458, 481, 498
SfaNI GCATC 1 cut(s) 129
SfcI CTRYAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 380, 537
SsiI CCGC 4 cut(s) 48, 289, 319, 361
SspI AATATT 1 cut(s) 511
TaiI ACGT 1 cut(s) 458
TaqI TCGA 2 cut(s) 73, 209
TasI AATT 2 cut(s) 380, 537
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 3 cut(s) 232, 285, 357
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 57
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 3 cut(s) 400, 439, 442
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 2 cut(s) 17, 539
TspGWI ACGGA 2 cut(s) 297, 531
TspRI CASTG 1 cut(s) 57
XapI RAATTY 2 cut(s) 380, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.