RLG00000025291

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007497
Physical Location & Seq
Reverse (-)
43652574 .. 43653164
591 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025291

Sequence Viewer

Length: 591 bp
ATGGGGCATGTATCAGCTGATCTTGGTGCTTGCCCAAAATGTGGTAGCCTAGAAGTTCCATACCCTTTTAGCACTGATGATAGTTGTGGAGACCCTAGATACAAAATCTACTGCAACAATGGCAACGAACTACAGTTCATGTCAGCTGAAGGGTTTAAGTACAAGATCCTCAGCATCGACTCGAGTGCCAACAAGCTCATTATACACCCACCTGATTTCTTAGTAGAGAATAACACTCCTACATGTTATTCTTCAGATTTCTCTTCACAAGGCCTAAGACTAGATGAACACTTGCCCTTCAACATTTCTACTCGTAACACCGTGATGCTGTTCAATTGCTCGGATAATATTCTCCTCTCGCCTCTAAATTGTTCCTCAAACAGCTTCTGCAGGCAGTTTGAGGATAAGATTGAGGCATGCAAAGGTACCCTTTGCTGCCACTTCTTGAAGGATTCCCGCATGACTTCATATATGATCAGGGTCAGGCTTGGAGGCTGCACTGCTTATACTTCTGTTGTCGATATTCGACCTCAAGATCCAGTTGATAACTGGAACTATGGAATTGAGCTTCAATGGTTGCCTCCTAACTAA

Protein Analysis

197

Amino Acids

22.01

Weight (kDa)

5.13

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 11 - 68 8.9e-12 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 425
AccB7I CCANNNNNTGG 1 cut(s) 41
AciI CCGC 1 cut(s) 457
AclWI GGATC 2 cut(s) 160, 530
AcuI CTGAAG 2 cut(s) 168, 237
AfaI GTAC 2 cut(s) 161, 427
AfiI CCNNNNNNNGG 1 cut(s) 41
AflIII ACRYGT 1 cut(s) 242
AgsI TTSAA 4 cut(s) 301, 334, 448, 572
AluBI AGCT 5 cut(s) 17, 146, 196, 384, 568
AluI AGCT 5 cut(s) 17, 146, 196, 384, 568
Alw26I GTCTC 1 cut(s) 84
AlwI GGATC 2 cut(s) 160, 530
AlwNI CAGNNNCTG 1 cut(s) 387
Ama87I CYCGRG 1 cut(s) 181
AoxI GGCC 1 cut(s) 271
ApeKI GCWGC 2 cut(s) 435, 495
Asp718I GGTACC 1 cut(s) 425
AvaI CYCGRG 1 cut(s) 181
BanI GGYRCC 1 cut(s) 425
BbvCI CCTCAGC 1 cut(s) 170
BbvI GCAGC 2 cut(s) 422, 482
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BclI TGATCA 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 84
BfaI CTAG 3 cut(s) 50, 96, 281
BfmI CTRYAG 2 cut(s) 131, 388
BisI GCNGC 2 cut(s) 436, 496
BlsI GCNGC 2 cut(s) 437, 497
BmeT110I CYCGRG 1 cut(s) 181
BmiI GGNNCC 1 cut(s) 427
BmsI GCATC 2 cut(s) 183, 315
Bpu10I CCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 516
BsaI GGTCTC 1 cut(s) 84
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse1I ACTGG 2 cut(s) 539, 554
BseLI CCNNNNNNNGG 1 cut(s) 41
BseMII CTCAG 1 cut(s) 184
BseNI ACTGG 2 cut(s) 539, 554
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 2 cut(s) 422, 482
BsgI GTGCAG 1 cut(s) 481
BshFI GGCC 1 cut(s) 273
BshNI GGYRCC 1 cut(s) 425
BsiHKCI CYCGRG 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 41
BsmAI GTCTC 1 cut(s) 84
BsnI GGCC 1 cut(s) 273
Bso31I GGTCTC 1 cut(s) 84
BsoBI CYCGRG 1 cut(s) 181
Bsp143I GATC 4 cut(s) 19, 165, 474, 535
BspACI CCGC 1 cut(s) 457
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 427
BspMAI CTGCAG 1 cut(s) 392
BspPI GGATC 2 cut(s) 160, 530
BspT107I GGYRCC 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 84
BsrI ACTGG 2 cut(s) 539, 554
BssMI GATC 4 cut(s) 19, 165, 474, 535
Bst4CI ACNGT 2 cut(s) 135, 322
Bst6I CTCTTC 1 cut(s) 268
BstC8I GCNNGC 3 cut(s) 31, 392, 418
BstDEI CTNAG 3 cut(s) 170, 220, 275
BstKTI GATC 4 cut(s) 22, 168, 477, 538
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 4 cut(s) 19, 165, 474, 535
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstNSI RCATGY 3 cut(s) 11, 246, 420
BstSFI CTRYAG 2 cut(s) 131, 388
BstV1I GCAGC 2 cut(s) 422, 482
BstX2I RGATCY 2 cut(s) 165, 535
BstYI RGATCY 2 cut(s) 165, 535
BsuRI GGCC 1 cut(s) 273
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 72, 498
Cac8I GCNNGC 3 cut(s) 31, 392, 418
CaiI CAGNNNCTG 1 cut(s) 387
Csp6I GTAC 2 cut(s) 160, 426
CviAII CATG 5 cut(s) 8, 139, 243, 417, 460
CviJI RGCY 9 cut(s) 17, 48, 146, 196, 273, 384, 487, 495, 568
CviKI_1 RGCY 9 cut(s) 17, 48, 146, 196, 273, 384, 487, 495, 568
CviQI GTAC 2 cut(s) 160, 426
DdeI CTNAG 3 cut(s) 170, 220, 275
DpnI GATC 4 cut(s) 21, 167, 476, 537
DpnII GATC 4 cut(s) 19, 165, 474, 535
Eam1104I CTCTTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 268
Eco147I AGGCCT 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 84
Eco57I CTGAAG 2 cut(s) 168, 237
Eco88I CYCGRG 1 cut(s) 181
FaeI CATG 5 cut(s) 11, 142, 246, 420, 463
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FatI CATG 5 cut(s) 7, 138, 242, 416, 459
FauI CCCGC 1 cut(s) 464
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 2 cut(s) 436, 496
Fsp4HI GCNGC 2 cut(s) 436, 496
FspBI CTAG 3 cut(s) 50, 96, 281
GluI GCNGC 2 cut(s) 436, 496
HaeIII GGCC 1 cut(s) 273
Hin1II CATG 5 cut(s) 11, 142, 246, 420, 463
HinfI GANTC 2 cut(s) 179, 452
Hpy188I TCNGA 2 cut(s) 256, 343
Hpy188III TCNNGA 2 cut(s) 445, 533
HpyAV CCTTC 3 cut(s) 143, 307, 442
HpyCH4III ACNGT 2 cut(s) 135, 322
HpyCH4V TGCA 4 cut(s) 114, 390, 420, 498
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
HpyF3I CTNAG 3 cut(s) 170, 220, 275
Hsp92II CATG 5 cut(s) 11, 142, 246, 420, 463
KpnI GGTACC 1 cut(s) 429
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 4 cut(s) 19, 165, 474, 535
LpnPI CCDG 6 cut(s) 225, 376, 463, 469, 535, 552
Lsp1109I GCAGC 2 cut(s) 422, 482
LweI GCATC 2 cut(s) 183, 315
MaeI CTAG 3 cut(s) 50, 96, 281
MaeIII GTNAC 1 cut(s) 314
MalI GATC 4 cut(s) 21, 167, 476, 537
MboI GATC 4 cut(s) 19, 165, 474, 535
MboII GAAGA 2 cut(s) 243, 255
MfeI CAATTG 1 cut(s) 334
MflI RGATCY 2 cut(s) 165, 535
MluCI AATT 3 cut(s) 334, 367, 561
MlyI GAGTC 1 cut(s) 173
MnlI CCTC 9 cut(s) 179, 365, 372, 385, 394, 406, 485, 540, 591
MseI TTAA 1 cut(s) 156
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 2 cut(s) 17, 146
MunI CAATTG 1 cut(s) 334
MwoI GCNNNNNNNGC 1 cut(s) 120
NdeII GATC 4 cut(s) 19, 165, 474, 535
NlaIII CATG 5 cut(s) 11, 142, 246, 420, 463
NlaIV GGNNCC 1 cut(s) 427
NspI RCATGY 3 cut(s) 11, 246, 420
PaeI GCATGC 1 cut(s) 420
PaeR7I CTCGAG 1 cut(s) 181
PceI AGGCCT 1 cut(s) 273
PciI ACATGT 1 cut(s) 242
PfeI GAWTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 2 cut(s) 437, 497
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PscI ACATGT 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 427
PspXI VCTCGAGB 1 cut(s) 181
PstI CTGCAG 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 387
PsuI RGATCY 2 cut(s) 165, 535
PvuII CAGCTG 2 cut(s) 17, 146
RsaI GTAC 2 cut(s) 161, 427
RsaNI GTAC 2 cut(s) 160, 426
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 2 cut(s) 436, 496
Sau3AI GATC 4 cut(s) 19, 165, 474, 535
SchI GAGTC 1 cut(s) 173
SetI ASST 8 cut(s) 19, 148, 198, 214, 386, 427, 532, 570
SfaNI GCATC 2 cut(s) 183, 315
SfcI CTRYAG 2 cut(s) 131, 388
Sfr274I CTCGAG 1 cut(s) 181
SlaI CTCGAG 1 cut(s) 181
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 2 cut(s) 181, 531
SmoI CTYRAG 2 cut(s) 181, 531
SphI GCATGC 1 cut(s) 420
Sse9I AATT 3 cut(s) 334, 367, 561
SseBI AGGCCT 1 cut(s) 273
SsiI CCGC 1 cut(s) 457
SspI AATATT 1 cut(s) 349
SspMI CTAG 3 cut(s) 50, 96, 281
StuI AGGCCT 1 cut(s) 273
TaaI ACNGT 2 cut(s) 135, 322
TaqI TCGA 4 cut(s) 177, 182, 519, 526
TasI AATT 3 cut(s) 334, 367, 561
TatI WGTACW 1 cut(s) 159
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 79, 505
TseI GCWGC 2 cut(s) 435, 495
TspDTI ATGAA 3 cut(s) 127, 300, 456
TspRI CASTG 2 cut(s) 79, 505
Van91I CCANNNNNTGG 1 cut(s) 41
XceI RCATGY 3 cut(s) 11, 246, 420
XcmI CCANNNNNNNNNTGG 1 cut(s) 546
XhoI CTCGAG 1 cut(s) 181
XspI CTAG 3 cut(s) 50, 96, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.