Rorug03G0014000

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007497
Physical Location & Seq
Reverse (-)
1101190 .. 1110757
9568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0014000.1

Sequence Viewer

Length: 2916 bp
ATGATGACGGTGCACGGCGAGGCGAGGCAGCCCCAGGCGGTGGCGTCTAGGGCTCAGTTTGGTGTGGAGTCTTTTAAGCCTGAGCCTTCTTCGCTCAGCTTAGTCTCCTTCAAGCCTCCTGATTCTCATGAAGTGGATGAGGATGCGCATTTGGCTATTGCTCACCAAATGTACAAGTCCGGCAACTATAAAGAGGCCTTGGAGCATAGCAATATCGTATATGAGAGGAATCCAATTCGAACTGATAACCTCCTTCTTTTGGGTGCAATATATTATCAGCTGCATGACTTTGATATGTGTATTGCCAAAAATGAAGAAGCCCTTCGAATTGAACCACATTTTGCTGAGTGTTATGGCAACATGGCCAATGCTTGGAAGGAAAAAGGCAATAGTGACCTTGCGATCCGCTACTATTTGATTGCCATTGAGCTTCGACCCAATTTCTGTGATGCATGGTCAAACTTAGCTAGTGCGTACATGCGGAAAGGAAGGCTTGAAGAGGCAGCCCAGTGCTGTCGTCAGGCACTTCAGCTGAATCCCCATTTGGTTGACGCCCATAGCAACCTTGGTAATTTAATGAAAGCACGAGGCTTGGTGCAAGAAGCATACAGTTGCTACCTCGAGGCTCTACGTATACAGCCAAATTTTGCTATTGCTTGGTCAAATCTTGCTGGCCTTTTCATGGAGTCTGGTGATCTTAATAGAGCTCTGCAGTATTATAAGGAGGCTGTGAAACTCAAACCTGCTTTTCCGGATGCGTATCTCAACCTTGGGAATGTTTATAAGGCTTTGGGATTGCCTCAAGAGGCAATTGTGTGTTATCAGCGTGCCCTGCAGACAAGACCTAACTATGCCATGGCTTTTGGTAACCTGGCCAGTACGTATTATGAGCAAGGTCAACTGGATTTGGCAGTCTTACATTACAAGCAAGCCATTGCTTGTGATCCAAGATTTTTGGAGGCTTACAATAATTTGGGTAACGCTTTGAAAGACATTGGCAGAGTGGACGAAGCAATTCAATGCTACAATCAATGCCTCACTCTACAACCTAATCATCCACAAGCACTTACCAACCTCGGGAATATTTATATGGAATGGAACATGGTAGCTGCTGCTGCTTCTTACTATAAGGCCACGTTGACTGTGACAACTGGGTTGTCTGCTCCTTTCAATAATCTTGCTATAATTTATAAACAACAGGGAAATTACGCAGATGCAATCTCTTGCTACAATGAGGTTCTTCGCATTGATCCTTTGGCAGCTGATGGGCTTGTCAATAGGGGCAACACTTACAAGGAGATTGGTAGAGTTAGTGAGGCAATTCAGGATTACATACATGCAATATCTGTCCGGCCAACTATGGCAGAAGCTCATGCAAACTTGGCTTCAGCTTACAAGGATAGTGGACATGTGGAGGCTGCTATTAAGAGCTATAAACAGGCATTACATCTTCGACCGGACTTTCCAGAGGCAACTTGTAATCTTTTACATACGTTACAGTGTGTGTGCAGTTGGGAGGACCGTGATAAAATGTTCGCTGAAGTTGAAGGGATTATCAGGAGGCAGATCAATATGTCTCTCTTGCCTAGTGTGCAGCCTTTCCACGCAATAGCATATCCTATTGATCCAATGCTTGCACTTGATATTAGTCGTAAATATGCTGCACAGTGCTCCATAATTGCATCCCGGTTTGGATTGACTGCCTTCAACCATCCTGCTCCAATTCCCATAAAGTGTAATGGTGGATTCGAGAGACTTAGAGTTGGATATGTAAGCAGTGACTTTGGTAATCACCCATTGTCACATCTTATGGGATCTGTTTTTGGGATGCACAATAAGGAGAATGTTGAGGTTTTCTGTTATGCCTTGAGTCCAAATGATGGAACGGAATGGAGACAGCGTACCCAGTCTGAAGCAGAGCACTTTGTTGATGTCTCATCTATGACATCAGATATGATTGCCAAAATGATTAATGAGGACAACATCCAGATTTTAATTAATCTTAATGGTTATACAAAGGGGGCGAGAAATGAAATATTTGCTATGCAGCCTGCACCTATTCAGGTTTCATACATGGGGTTCCCTGGGACCACTGGGGCAAATTACATTGATTACTTGGTCACTGATGAGTTTGTCTCACCTTTGCGCTTCTCACATATTTACTCTGAGAAGCTTGTTCATCTTCCACATTGCTACTTTGTGAATGACTATAAGCAGAAAAATCTGGATGTATTGGATCCAAATTGTCGGCACAAAAGATCAGATTATGGTCTCCCAGAGGATAAATTTATATTTGCAACCTTCAATCAGTTGTACAAAATGGATCCTGAAATATTTAATACTTGGTGCAATATTCTTAAACGTGTACCCAACAGTGCACTCTGGCTCCTCAGATTCCCAGCTGCAGGCGAAATGAGACTCCGTACATATGCTGCTGCTCAAGGAGTGCAAGCAGACCAGATTATTTTTACAGATGTTGCTATGAAAGGTGAACATATCAGGCGCAGTGCTTTAGCAGATTTGTTCCTTGACACGCCTTTATGCAATGCACATACAACCGGCACAGATGTTCTATGGGCCGGTTTACCAATGGTGACCCTTCCCCTTGAGAAGATGGCCACCAGGGTTGCTGGATCTTTGTGTCTCGCCACTGGACTGGGGGAGGAGATGATAGTTAGCAGTATGAAAGAGTATGAAGAGAAGGCAGTATCTTTGGCATTGAATCCCTCAAAGCTCCAGGCGCTTACCAACAAACTCAAGGCAGTCCGAATGACTTGCCCTCTGTTTGACACAACACGCTGGGTGAGGAATCTGGAAAGGGCTTATTTCAAAATGTGGAATCTGCATTGCTCAGGCCAGCGTCCCCAGCATTTTAAAGTTGCTGAGAATGATTCGGATTTCCCTTATGATAGATAG

Protein Analysis

971

Amino Acids

109.42

Weight (kDa)

6.22

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_16 PF13432 127 - 180 2.7e-07 Tetratricopeptide repeat
TPR_8 PF13181 150 - 180 2.8e-06 Tetratricopeptide repeat
TPR_1 PF00515 150 - 181 7e-10 Tetratricopeptide repeat
TPR_2 PF07719 150 - 181 1.2e-08 Tetratricopeptide repeat
TPR_11 PF13414 157 - 195 3.1e-08 TPR repeat
TPR_1 PF00515 217 - 248 9e-06 Tetratricopeptide repeat
TPR_11 PF13414 227 - 265 5.8e-11 TPR repeat
TPR_19 PF14559 228 - 284 3.8e-08 Tetratricopeptide repeat
TPR_1 PF00515 251 - 284 7.3e-10 Tetratricopeptide repeat
TPR_2 PF07719 251 - 284 5.4e-06 Tetratricopeptide repeat
TPR_12 PF13424 253 - 314 1.4e-10 Tetratricopeptide repeat
TPR_16 PF13432 255 - 316 2.2e-06 Tetratricopeptide repeat
TPR_11 PF13414 269 - 299 9.8e-06 TPR repeat
TPR_1 PF00515 285 - 318 5.3e-06 Tetratricopeptide repeat
TPR_11 PF13414 294 - 333 2.7e-10 TPR repeat
TPR_17 PF13431 307 - 340 3.5e-07 Tetratricopeptide repeat
TPR_12 PF13424 319 - 381 7.9e-07 Tetratricopeptide repeat
TPR_1 PF00515 320 - 352 1.4e-09 Tetratricopeptide repeat
TPR_8 PF13181 320 - 352 2.8e-08 Tetratricopeptide repeat
TPR_2 PF07719 320 - 352 4.2e-07 Tetratricopeptide repeat
TPR_11 PF13414 326 - 364 1.2e-08 TPR repeat
TPR_1 PF00515 390 - 418 6.6e-08 Tetratricopeptide repeat
TPR_2 PF07719 391 - 418 1.5e-06 Tetratricopeptide repeat
TPR_9 PF13371 395 - 458 3.6e-06 Tetratricopeptide repeat
TPR_11 PF13414 396 - 435 1.6e-07 TPR repeat
TPR_11 PF13414 428 - 469 2.6e-07 TPR repeat
TPR_1 PF00515 455 - 488 2.8e-06 Tetratricopeptide repeat
TPR_11 PF13414 463 - 495 1.6e-06 TPR repeat
Glyco_transf_41 PF13844 502 - 739 2.5e-83 Glycosyl transferase family 41
Glyco_transf_41 PF13844 752 - 938 1.8e-68 Glycosyl transferase family 41
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 720, 783, 1191
AbsI CCTCGAGG 1 cut(s) 620
Acc16I TGCGCA 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 751
AccB7I CCANNNNNTGG 3 cut(s) 40, 372, 1880
AccI GTMKAC 1 cut(s) 634
AccIII TCCGGA 1 cut(s) 751
AciI CCGC 3 cut(s) 38, 406, 481
AcoI YGGCCR 4 cut(s) 363, 873, 1352, 2616
AcsI RAATTY 2 cut(s) 643, 2285
AcuI CTGAAG 4 cut(s) 512, 1371, 1560, 1932
AcyI GRCGYC 2 cut(s) 44, 552
AfaI GTAC 7 cut(s) 173, 476, 880, 1903, 2315, 2367, 2425
AfiI CCNNNNNNNGG 7 cut(s) 40, 259, 372, 682, 1077, 1880, 2405
AflIII ACRYGT 2 cut(s) 1408, 2362
AjnI CCWGG 5 cut(s) 33, 870, 2083, 2621, 2736
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
Alw21I GWGCWC 5 cut(s) 15, 709, 1673, 1923, 2380
Alw26I GTCTC 9 cut(s) 109, 1581, 1747, 1888, 1939, 2140, 2276, 2410, 2648
Alw44I GTGCAC 2 cut(s) 11, 2376
Ama87I CYCGRG 2 cut(s) 620, 1076
Aor13HI TCCGGA 1 cut(s) 751
AoxI GGCC 9 cut(s) 195, 363, 673, 873, 1131, 1352, 2577, 2616, 2854
ApaLI GTGCAC 2 cut(s) 11, 2376
ApoI RAATTY 2 cut(s) 643, 2285
AseI ATTAAT 2 cut(s) 1971, 1998
Asp700I GAANNNNTTC 2 cut(s) 321, 1014
AspLEI GCGC 4 cut(s) 148, 2148, 2505, 2743
AspS9I GGNCC 3 cut(s) 1519, 2088, 2577
AsuC2I CCSGG 1 cut(s) 1687
AsuHPI GGTGA 7 cut(s) 155, 704, 1784, 2130, 2501, 2605, 2815
AsuII TTCGAA 2 cut(s) 238, 325
AvaI CYCGRG 2 cut(s) 620, 1076
AvaII GGWCC 2 cut(s) 1519, 2088
BaeGI GKGCMC 3 cut(s) 15, 832, 2380
BalI TGGCCA 3 cut(s) 365, 875, 2618
BamHI GGATCC 2 cut(s) 2236, 2323
BanII GRGCYC 2 cut(s) 55, 709
BauI CACGAG 1 cut(s) 585
Bbv12I GWGCWC 5 cut(s) 15, 709, 1673, 1923, 2380
BccI CCATC 4 cut(s) 1259, 1719, 1874, 2608
BceAI ACGGC 1 cut(s) 31
BcgI CGANNNNNNTGC 2 cut(s) 2757, 2791
BciT130I CCWGG 5 cut(s) 35, 872, 2085, 2623, 2738
BcnI CCSGG 1 cut(s) 1687
BcoDI GTCTC 9 cut(s) 109, 1581, 1747, 1888, 1939, 2140, 2276, 2410, 2648
BfaI CTAG 3 cut(s) 48, 468, 1587
BfmI CTRYAG 3 cut(s) 710, 833, 2403
BfoI RGCGCY 1 cut(s) 2744
BfuAI ACCTGC 1 cut(s) 751
BlpI GCTNAGC 1 cut(s) 95
Bme1390I CCNGG 6 cut(s) 35, 872, 1687, 2085, 2623, 2738
Bme18I GGWCC 2 cut(s) 1519, 2088
BmeT110I CYCGRG 2 cut(s) 620, 1076
BmgT120I GGNCC 3 cut(s) 1519, 2088, 2577
BmiI GGNNCC 5 cut(s) 2081, 2089, 2238, 2325, 2387
BmrFI CCNGG 6 cut(s) 35, 872, 1687, 2085, 2623, 2738
BmrI ACTGGG 5 cut(s) 502, 1161, 1900, 2103, 2666
BmsI GCATC 6 cut(s) 133, 439, 745, 1204, 1691, 1818
BmuI ACTGGG 5 cut(s) 502, 1161, 1900, 2103, 2666
BplI GAGNNNNNCTC 2 cut(s) 2120, 2152
BpmI CTGGAG 1 cut(s) 2720
Bpu10I CCTNAGC 2 cut(s) 81, 2851
Bpu1102I GCTNAGC 1 cut(s) 95
Bpu14I TTCGAA 2 cut(s) 238, 325
BpuEI CTTGAG 5 cut(s) 786, 1888, 2424, 2627, 2741
BpuMI CCSGG 1 cut(s) 1687
BsaAI YACGTR 2 cut(s) 632, 882
BsaBI GATNNNNATC 1 cut(s) 759
BsaHI GRCGYC 2 cut(s) 44, 552
BsaI GGTCTC 1 cut(s) 2276
BsaJI CCNNGG 9 cut(s) 33, 198, 565, 769, 855, 1075, 2083, 2084, 2622
BsaWI WCCGGW 2 cut(s) 751, 1456
BsaXI ACNNNNNCTCC 4 cut(s) 1885, 1915, 2370, 2400
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 259, 372, 682, 1077, 1880, 2405
Bse118I RCCGGY 2 cut(s) 2558, 2579
Bse1I ACTGG 8 cut(s) 508, 876, 906, 1156, 1906, 2098, 2656, 2661
Bse3DI GCAATG 4 cut(s) 933, 2188, 2551, 2845
Bse8I GATNNNNATC 1 cut(s) 759
BseAI TCCGGA 1 cut(s) 751
BseBI CCWGG 5 cut(s) 35, 872, 2085, 2623, 2738
BseDI CCNNGG 9 cut(s) 33, 198, 565, 769, 855, 1075, 2083, 2084, 2622
BseGI GGATG 9 cut(s) 142, 148, 760, 1054, 1682, 1711, 1833, 1983, 2233
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 7 cut(s) 40, 259, 372, 682, 1077, 1880, 2405
BseMI GCAATG 4 cut(s) 933, 2188, 2551, 2845
BseMII CTCAG 8 cut(s) 68, 72, 109, 336, 2157, 2404, 2865, 2874
BseNI ACTGG 8 cut(s) 508, 876, 906, 1156, 1906, 2098, 2656, 2661
BseRI GAGGAG 2 cut(s) 2378, 2678
BseSI GKGCMC 3 cut(s) 15, 832, 2380
BseYI CCCAGC 3 cut(s) 2398, 2799, 2865
BsgI GTGCAG 4 cut(s) 1528, 1613, 1647, 2037
Bsh1285I CGRYCG 1 cut(s) 1457
BshFI GGCC 9 cut(s) 197, 365, 675, 875, 1133, 1354, 2579, 2618, 2856
BsiEI CGRYCG 1 cut(s) 1457
BsiHKAI GWGCWC 5 cut(s) 15, 709, 1673, 1923, 2380
BsiHKCI CYCGRG 2 cut(s) 620, 1076
BsiSI CCGG 7 cut(s) 180, 752, 1351, 1457, 1687, 2559, 2580
BslFI GGGAC 2 cut(s) 2101, 2847
BslI CCNNNNNNNGG 7 cut(s) 40, 259, 372, 682, 1077, 1880, 2405
BsmAI GTCTC 9 cut(s) 109, 1581, 1747, 1888, 1939, 2140, 2276, 2410, 2648
BsmFI GGGAC 2 cut(s) 2101, 2847
BsnI GGCC 9 cut(s) 197, 365, 675, 875, 1133, 1354, 2579, 2618, 2856
Bso31I GGTCTC 1 cut(s) 2276
BsoBI CYCGRG 2 cut(s) 620, 1076
Bsp119I TTCGAA 2 cut(s) 238, 325
Bsp1286I GDGCHC 7 cut(s) 15, 55, 709, 832, 1673, 1923, 2380
Bsp13I TCCGGA 1 cut(s) 751
Bsp1407I TGTACA 2 cut(s) 171, 2313
Bsp1720I GCTNAGC 1 cut(s) 95
Bsp19I CCATGG 1 cut(s) 855
BspACI CCGC 3 cut(s) 38, 406, 481
BspANI GGCC 9 cut(s) 197, 365, 675, 875, 1133, 1354, 2579, 2618, 2856
BspCNI CTCAG 8 cut(s) 67, 73, 108, 337, 2158, 2403, 2864, 2875
BspEI TCCGGA 1 cut(s) 751
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 5 cut(s) 2081, 2089, 2238, 2325, 2387
BspMAI CTGCAG 3 cut(s) 714, 837, 2407
BspMI ACCTGC 1 cut(s) 751
BspT104I TTCGAA 2 cut(s) 238, 325
BspTNI GGTCTC 1 cut(s) 2276
BsrDI GCAATG 4 cut(s) 933, 2188, 2551, 2845
BsrFI RCCGGY 2 cut(s) 2558, 2579
BsrGI TGTACA 2 cut(s) 171, 2313
BsrI ACTGG 8 cut(s) 508, 876, 906, 1156, 1906, 2098, 2656, 2661
BssAI RCCGGY 2 cut(s) 2558, 2579
BssECI CCNNGG 9 cut(s) 33, 198, 565, 769, 855, 1075, 2083, 2084, 2622
BssNAI GTATAC 1 cut(s) 635
BssNI GRCGYC 2 cut(s) 44, 552
BssSI CACGAG 1 cut(s) 585
BssT1I CCWWGG 4 cut(s) 198, 565, 769, 855
Bst1107I GTATAC 1 cut(s) 635
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 5 cut(s) 35, 872, 2085, 2623, 2738
Bst4CI ACNGT 7 cut(s) 10, 611, 1144, 1500, 1523, 1668, 2375
Bst6I CTCTTC 2 cut(s) 492, 2691
BstACI GRCGYC 2 cut(s) 44, 552
BstAUI TGTACA 2 cut(s) 171, 2313
BstBAI YACGTR 2 cut(s) 632, 882
BstBI TTCGAA 2 cut(s) 238, 325
BstC8I GCNNGC 8 cut(s) 673, 828, 930, 1635, 2052, 2407, 2451, 2858
BstDSI CCRYGG 1 cut(s) 855
BstEII GGTNACC 2 cut(s) 866, 2593
BstF5I GGATG 9 cut(s) 142, 148, 760, 1054, 1682, 1711, 1833, 1983, 2233
BstH2I RGCGCY 1 cut(s) 2744
BstHHI GCGC 4 cut(s) 148, 2148, 2505, 2743
BstMAI GTCTC 9 cut(s) 109, 1581, 1747, 1888, 1939, 2140, 2276, 2410, 2648
BstMCI CGRYCG 1 cut(s) 1457
BstMWI GCNNNNNNNGC 9 cut(s) 50, 91, 152, 832, 1115, 1382, 1591, 2740, 2866
BstNI CCWGG 5 cut(s) 35, 872, 2085, 2623, 2738
BstNSI RCATGY 3 cut(s) 481, 1340, 1412
BstPI GGTNACC 2 cut(s) 866, 2593
BstSCI CCNGG 6 cut(s) 33, 870, 1685, 2083, 2621, 2736
BstSFI CTRYAG 3 cut(s) 710, 833, 2403
BstSLI GKGCMC 3 cut(s) 15, 832, 2380
BstSNI TACGTA 2 cut(s) 632, 882
BstX2I RGATCY 4 cut(s) 1814, 2236, 2323, 2633
BstXI CCANNNNNNTGG 1 cut(s) 2656
BstYI RGATCY 4 cut(s) 1814, 2236, 2323, 2633
BstZ17I GTATAC 1 cut(s) 635
BsuRI GGCC 9 cut(s) 197, 365, 675, 875, 1133, 1354, 2579, 2618, 2856
BtgI CCRYGG 1 cut(s) 855
BtsCI GGATG 9 cut(s) 142, 148, 760, 1054, 1682, 1711, 1833, 1983, 2233
BtsI GCAGTG 2 cut(s) 1783, 2512
BtsIMutI CAGTG 9 cut(s) 515, 1505, 1673, 1783, 2091, 2121, 2380, 2512, 2649
BveI ACCTGC 1 cut(s) 751
Cac8I GCNNGC 8 cut(s) 673, 828, 930, 1635, 2052, 2407, 2451, 2858
CciI TCATGA 1 cut(s) 127
CfoI GCGC 4 cut(s) 148, 2148, 2505, 2743
Cfr10I RCCGGY 2 cut(s) 2558, 2579
Cfr13I GGNCC 3 cut(s) 1519, 2088, 2577
CseI GACGC 3 cut(s) 33, 560, 2849
Csp6I GTAC 7 cut(s) 172, 475, 879, 1902, 2314, 2366, 2424
CspCI CAANNNNNGTGG 4 cut(s) 1384, 1419, 2608, 2643
CviQI GTAC 7 cut(s) 172, 475, 879, 1902, 2314, 2366, 2424
DraI TTTAAA 1 cut(s) 2875
EaeI YGGCCR 4 cut(s) 363, 873, 1352, 2616
Eam1104I CTCTTC 2 cut(s) 492, 2691
EarI CTCTTC 2 cut(s) 492, 2691
Ecl136II GAGCTC 1 cut(s) 707
Eco105I TACGTA 2 cut(s) 632, 882
Eco130I CCWWGG 4 cut(s) 198, 565, 769, 855
Eco147I AGGCCT 1 cut(s) 197
Eco24I GRGCYC 2 cut(s) 55, 709
Eco31I GGTCTC 1 cut(s) 2276
Eco47I GGWCC 2 cut(s) 1519, 2088
Eco53kI GAGCTC 1 cut(s) 707
Eco57I CTGAAG 4 cut(s) 512, 1371, 1560, 1932
Eco88I CYCGRG 2 cut(s) 620, 1076
Eco91I GGTNACC 2 cut(s) 866, 2593
EcoICRI GAGCTC 1 cut(s) 707
EcoO65I GGTNACC 2 cut(s) 866, 2593
EcoRII CCWGG 5 cut(s) 33, 870, 2083, 2621, 2736
EcoT14I CCWWGG 4 cut(s) 198, 565, 769, 855
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 2 cut(s) 55, 709
ErhI CCWWGG 4 cut(s) 198, 565, 769, 855
FalI AAGNNNNNCTT 4 cut(s) 306, 338, 477, 509
FaqI GGGAC 2 cut(s) 2101, 2847
FauNDI CATATG 1 cut(s) 2428
FblI GTMKAC 1 cut(s) 634
FokI GGATG 9 cut(s) 149, 155, 767, 1041, 1669, 1698, 1840, 1970, 2240
FriOI GRGCYC 2 cut(s) 55, 709
FspAI RTGCGCAY 1 cut(s) 147
FspBI CTAG 3 cut(s) 48, 468, 1587
FspI TGCGCA 1 cut(s) 147
GlaI GCGC 4 cut(s) 147, 2147, 2504, 2742
GsaI CCCAGC 3 cut(s) 2402, 2803, 2869
GsuI CTGGAG 1 cut(s) 2720
HaeII RGCGCY 1 cut(s) 2744
HaeIII GGCC 9 cut(s) 197, 365, 675, 875, 1133, 1354, 2579, 2618, 2856
HapII CCGG 7 cut(s) 180, 752, 1351, 1457, 1687, 2559, 2580
HgaI GACGC 3 cut(s) 33, 560, 2849
HhaI GCGC 4 cut(s) 148, 2148, 2505, 2743
Hin1I GRCGYC 2 cut(s) 44, 552
Hin6I GCGC 4 cut(s) 146, 2146, 2503, 2741
HinP1I GCGC 4 cut(s) 146, 2146, 2503, 2741
HincII GTYRAC 3 cut(s) 550, 899, 1140
HindII GTYRAC 3 cut(s) 550, 899, 1140
HindIII AAGCTT 1 cut(s) 2171
HpaII CCGG 7 cut(s) 180, 752, 1351, 1457, 1687, 2559, 2580
HphI GGTGA 7 cut(s) 155, 704, 1784, 2130, 2501, 2605, 2815
Hpy188I TCNGA 7 cut(s) 1912, 1951, 2167, 2263, 2393, 2768, 2896
HpyCH4III ACNGT 7 cut(s) 10, 611, 1144, 1500, 1523, 1668, 2375
HpyCH4IV ACGT 5 cut(s) 631, 881, 1136, 1493, 2362
HpyF10VI GCNNNNNNNGC 9 cut(s) 50, 91, 152, 832, 1115, 1382, 1591, 2740, 2866
HpySE526I ACGT 5 cut(s) 631, 881, 1136, 1493, 2362
Hsp92I GRCGYC 2 cut(s) 44, 552
HspAI GCGC 4 cut(s) 146, 2146, 2503, 2741
Kpn2I TCCGGA 1 cut(s) 751
LmnI GCTCC 6 cut(s) 202, 1168, 1676, 1723, 2391, 2739
LweI GCATC 6 cut(s) 133, 439, 745, 1204, 1691, 1818
MaeI CTAG 3 cut(s) 48, 468, 1587
MaeII ACGT 5 cut(s) 631, 881, 1136, 1493, 2362
MaeIII GTNAC 9 cut(s) 392, 866, 977, 1144, 1494, 1778, 1800, 2119, 2593
MboII GAAGA 8 cut(s) 81, 326, 509, 1232, 1442, 2174, 2623, 2708
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 4 cut(s) 1814, 2236, 2323, 2633
MhlI GDGCHC 7 cut(s) 15, 55, 709, 832, 1673, 1923, 2380
MlsI TGGCCA 3 cut(s) 365, 875, 2618
MluNI TGGCCA 3 cut(s) 365, 875, 2618
MlyI GAGTC 4 cut(s) 77, 695, 1879, 2412
MmeI TCCRAC 1 cut(s) 1744
Mox20I TGGCCA 3 cut(s) 365, 875, 2618
Mph1103I ATGCAT 1 cut(s) 454
MroI TCCGGA 1 cut(s) 751
MroXI GAANNNNTTC 2 cut(s) 321, 1014
MscI TGGCCA 3 cut(s) 365, 875, 2618
Msp20I TGGCCA 3 cut(s) 365, 875, 2618
MspA1I CMGCKG 4 cut(s) 280, 532, 1262, 2402
MspI CCGG 7 cut(s) 180, 752, 1351, 1457, 1687, 2559, 2580
MspR9I CCNGG 6 cut(s) 35, 872, 1687, 2085, 2623, 2738
MunI CAATTG 1 cut(s) 810
MvaI CCWGG 5 cut(s) 35, 872, 2085, 2623, 2738
MwoI GCNNNNNNNGC 9 cut(s) 50, 91, 152, 832, 1115, 1382, 1591, 2740, 2866
NciI CCSGG 1 cut(s) 1687
NcoI CCATGG 1 cut(s) 855
NdeI CATATG 1 cut(s) 2428
NlaIV GGNNCC 5 cut(s) 2081, 2089, 2238, 2325, 2387
NmuCI GTSAC 6 cut(s) 392, 1144, 1778, 1800, 2119, 2593
NsbI TGCGCA 1 cut(s) 147
NsiI ATGCAT 1 cut(s) 454
NspI RCATGY 3 cut(s) 481, 1340, 1412
NspV TTCGAA 2 cut(s) 238, 325
PacI TTAATTAA 1 cut(s) 1998
PaeR7I CTCGAG 1 cut(s) 620
PagI TCATGA 1 cut(s) 127
PasI CCCWGGG 1 cut(s) 2084
PceI AGGCCT 1 cut(s) 197
PciI ACATGT 1 cut(s) 1408
PdmI GAANNNNTTC 2 cut(s) 321, 1014
PfeI GAWTC 9 cut(s) 122, 229, 535, 1746, 2394, 2722, 2809, 2839, 2891
PflMI CCANNNNNTGG 3 cut(s) 40, 372, 1880
PleI GAGTC 4 cut(s) 76, 694, 1878, 2412
PpsI GAGTC 4 cut(s) 76, 694, 1878, 2412
Ppu21I YACGTR 2 cut(s) 632, 882
PscI ACATGT 1 cut(s) 1408
PshBI ATTAAT 2 cut(s) 1971, 1998
PsiI TTATAA 3 cut(s) 720, 783, 1191
Psp124BI GAGCTC 1 cut(s) 709
Psp6I CCWGG 5 cut(s) 33, 870, 2083, 2621, 2736
PspEI GGTNACC 2 cut(s) 866, 2593
PspFI CCCAGC 3 cut(s) 2398, 2799, 2865
PspGI CCWGG 5 cut(s) 33, 870, 2083, 2621, 2736
PspN4I GGNNCC 5 cut(s) 2081, 2089, 2238, 2325, 2387
PspPI GGNCC 3 cut(s) 1519, 2088, 2577
PspXI VCTCGAGB 1 cut(s) 620
PstI CTGCAG 3 cut(s) 714, 837, 2407
PsuI RGATCY 4 cut(s) 1814, 2236, 2323, 2633
PvuII CAGCTG 4 cut(s) 280, 532, 1262, 2402
RsaI GTAC 7 cut(s) 173, 476, 880, 1903, 2315, 2367, 2425
RsaNI GTAC 7 cut(s) 172, 475, 879, 1902, 2314, 2366, 2424
SacI GAGCTC 1 cut(s) 709
Sau96I GGNCC 3 cut(s) 1519, 2088, 2577
SchI GAGTC 4 cut(s) 77, 695, 1879, 2412
ScrFI CCNGG 6 cut(s) 35, 872, 1687, 2085, 2623, 2738
SduI GDGCHC 7 cut(s) 15, 55, 709, 832, 1673, 1923, 2380
SfaNI GCATC 6 cut(s) 133, 439, 745, 1204, 1691, 1818
SfcI CTRYAG 3 cut(s) 710, 833, 2403
Sfr274I CTCGAG 1 cut(s) 620
SfuI TTCGAA 2 cut(s) 238, 325
SinI GGWCC 2 cut(s) 1519, 2088
SlaI CTCGAG 1 cut(s) 620
SmlI CTYRAG 6 cut(s) 620, 801, 1867, 2439, 2606, 2756
SmoI CTYRAG 6 cut(s) 620, 801, 1867, 2439, 2606, 2756
SnaBI TACGTA 2 cut(s) 632, 882
SseBI AGGCCT 1 cut(s) 197
SsiI CCGC 3 cut(s) 38, 406, 481
SspI AATATT 4 cut(s) 1084, 2037, 2334, 2353
SspMI CTAG 3 cut(s) 48, 468, 1587
SstI GAGCTC 1 cut(s) 709
StuI AGGCCT 1 cut(s) 197
StyD4I CCNGG 6 cut(s) 33, 870, 1685, 2083, 2621, 2736
StyI CCWWGG 4 cut(s) 198, 565, 769, 855
TaaI ACNGT 7 cut(s) 10, 611, 1144, 1500, 1523, 1668, 2375
TaiI ACGT 5 cut(s) 634, 884, 1139, 1496, 2365
TaqI TCGA 6 cut(s) 238, 325, 433, 621, 1453, 1749
TatI WGTACW 2 cut(s) 171, 2313
TfiI GAWTC 9 cut(s) 122, 229, 535, 1746, 2394, 2722, 2809, 2839, 2891
TscAI CASTG 9 cut(s) 515, 1505, 1673, 1783, 2098, 2128, 2380, 2512, 2656
TseFI GTSAC 6 cut(s) 392, 1144, 1778, 1800, 2119, 2593
Tsp45I GTSAC 6 cut(s) 392, 1144, 1778, 1800, 2119, 2593
TspGWI ACGGA 2 cut(s) 1901, 2411
TspRI CASTG 9 cut(s) 515, 1505, 1673, 1783, 2098, 2128, 2380, 2512, 2656
Van91I CCANNNNNTGG 3 cut(s) 40, 372, 1880
VneI GTGCAC 2 cut(s) 11, 2376
VpaK11BI GGWCC 2 cut(s) 1519, 2088
VspI ATTAAT 2 cut(s) 1971, 1998
XapI RAATTY 2 cut(s) 643, 2285
XceI RCATGY 3 cut(s) 481, 1340, 1412
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XhoI CTCGAG 1 cut(s) 620
XmiI GTMKAC 1 cut(s) 634
XmnI GAANNNNTTC 2 cut(s) 321, 1014
XspI CTAG 3 cut(s) 48, 468, 1587
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.