Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0003593

Physical Location & Seq

Genomic Coordinates Reverse (-)

3413397 .. 3417822

Gene Structure 4426 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000026498

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGGCTTCCAACAACGTCACCAAAACCCCTGAACGGCGCTCCAACACATCGCGAAATGTCCGACTCGTGGCGAAGATCGGCTGCGTTGCAGATCCGGCTGCCGAGGCTTCCGGTGGTGTGTCGTGGATCTCGGTGAACAAGCCCGCCGGAGAGGCGTCGGACGGTGTCACTCTCTCTTTCAGAGACCAATCAGCGAGCCGTAAAGAGTCCTATGAATTGGATTATTGCTATGACCAAAATGAGGATAATGGCCTAATTTTTTTGCGAGAAGTAAAGCCTTTAATTCCTGGAGTTCTGGAGGGCTGTAATGCCACTGTCATTGGTTTGGGAGCTAAAGGTAGTGGAAAGACTAGCTTAATTCAGGGTTCGAGTGAGAAACCAGGTTTGGCAACAATGGCAACCGAGGAAATTATTTCAATGGCTGAGAAAAACGGATGTTCAATTACTATATCCTCATATGAGGTTTATCAGGACCACATTTATGATATATTGGATCCATCACGCCCAGAAGTTTTAGTTCTGGGAGACACTCAAGGAAACATTCGGCTTAAAGGACTTTCTCAGGTGCCTGTGAAGTCCATGTGGGAATTTAGCAAACTGTATGTTAGTGGGGGTACTTCCCGTAAACCAGCACCAAAGGGACCAGCTGAACTTTCTCGTAGGTGCCACAAGGGATTGATAGTATATGTATCATCTCAAAGAGAAAATTCAGATACCCTTCAAGTCAGCAAGTTAAACTTTATTGACTTAGCAGGTTATGAAGATACAAGAAGGAAGAGCATTGACTGCGTTAATCATGTTGAGAGCAGTAAAATCAATAAGTCAATCTATGCCATGATCAATGTTGTTAATGCAGTGAGTTCCAATGGAAACCATGTGCCTTATCGGGAAAGCAAACTCACACATTTATTGCAAGACTCCCTGGGAGGGATGAGTAGAGTTCTGATGATCACTTGCTTGAAACCGTCATTTTGCCAAGATTCTATTTACATGGTAAGCTTGGTGTCTCGCTCTTCTCAAAACACCAATCAGGCTGTCACAGTCTCAACAAAGAAAATTAAAGGTCTGACAAGATTAGAAGTGCGTTCATCACAGAAGGGCCATGTATCCATGACTACTTCTGCTATAGCAACGAAACAAACTGTCTCCCGAGTGCCTCTTTCTGAGAAGAAAGTCAATGGGCCTCTTTCTTCCAAGAAAGCAAATAGTAGTATGACTTCCTCATTGAAAGGAAGGAAACTATTTGATGAAAGAAGTCATTTGACCAAATCGATGAAGGCAAGTGCTATGTCAGATATTGTCTCAACCATTGAAACTTTTGTAGCTGATAAGGAAGAGACCATTTCAAATGTTCCTGAAGGAACCGAGCAGCTTAAATCTGAGAATCCATCATCAAATGCTTTGAATCCCGTAGAACCTGCATCTCTTATGGAAAAGGACAAATTATTATCAGATGATCCAAAGCATGCAGAACATTCTTTGAAGGAAGAAAAGGAGCATTTGCTATTGGATGATCCAAAGCATGCTGAAGATAATTCAATGGCAGAAAAGGATGTTTCTGTGAATGGTGATGGCCCTACTGAGGAAGTTAATCGTAGTGACAATAGCAGTAGCAGATATGCATTGGCTTTAGTTGAAGAAGTTCATGACATGGAGAAGGAAAACAATGACTTCCCAGACAACGAAGATGCATCACCTCCAATCAGTGCACGACTACAAGAATTGGCATTAAAAATGAAATCACTTTGTTCTTCAACTCCATTGTGCATTACGATGCCAGAGGAGAAAGATACTTCATTCAATAACTTCCTCTGTACTGACATCGTGGAATCAAACGCCCCAGTTGCCAGTCAGGATATAAGCCACAATGATAGTTGGGAGGTTGCAAATGTCAACAACATCATGGAACCAAAGACTCCAGTTGTGGAACCAAAAACCCCAGTTGCTAATCAGGATATAAAACACAATGACAGATGGGAGGTTGCAAATGTCAACAGCCCTTGGGAAACGTTGAGTGTCCGCAGTACTGGTGTCAAGCAATCTCTTGTTCAAGAATATCTTAGCTTTTTAAACACCGCTAGCAAGGAAGATCTGAAAAGATTAAAGGGAATTGGAGAGAAAAGAGCAACATACATACTTGATTGCCGTGAAGAAACTCCTGAGCCCTTCAAGAGTCTTGATGATTTACAAGACATTGGACTTTCAGTGAAGCAGATTAAGAACTTGATGAAGAAAGCAGGAGGAGGGCTTTTCGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000070 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007017 GO:0007018 GO:0007049 GO:0007059 GO:0007062 GO:0007080 GO:0008104 GO:0008150 GO:0008574 GO:0009987 GO:0015630 GO:0016043 GO:0016192 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0030705 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0042623 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046907 GO:0048002 GO:0048193 GO:0048285 GO:0050000 GO:0051179 GO:0051234 GO:0051276 GO:0051303 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0070013 GO:0071840 GO:0072686 GO:0097159 GO:0098687 GO:0098813 GO:0140014 GO:1901363 GO:1903047 GO:1990939

KEGG Pathways

Metabolic & Signaling

ko04914 map04914

Pfam Domains

Protein Families

HHH_3 Kinesin

Protein Analysis

753

Amino Acids

82.44

Weight (kDa)

5.78

Isoelectric Point (pI)

48.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	59 - 323	2.1e-60	Kinesin motor domain
Microtub_bd	PF16796	59 - 164	5.3e-09	Microtubule binding
HHH_3	PF12836	690 - 732	1.5e-07	Helix-hairpin-helix motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003593)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23910 AT5G23910
fragaria_vesca	FvH4_3g27740 FvH4_7g29790 FvH4_7g29790 FvH4_7g29790 FvH4_7g29790 FvH4_7g29790 FvH4_7g29790 FvH4_7g29790 FvH4_7g29790
malus_domestica	MD01G1204800.v1.1 MD07G1272100.v1.1
prunus_persica	Prupe.2G297200_v2.0.a1
pyrus_communis	pycom01g21440
rosa_chinensis	RchiOBHm_Chr1g0377791
rosa_laevigata	RLG00000019998 RLG00000026498
rosa_multiflora	Rmu_sc0004824.1_g000001 Rmu_sc0005758.1_g000004 Rmu_sc0024583.1_g000001 Rmu_sc0028648.1_g000003
rosa_roxburghii	Rroxscaffold_4G00281060
rosa_rugosa	Rorug01G0405100 Rorug02G0377300
rosa_samantha	Rh1AG427300 Rh1BG382000 Rh1CG395900 Rh1DG413000 Rh2AG430200 Rh2BG439200 Rh2CG415800 Rh2DG449200
rosa_wichuraiana	Rw1G037130 Rw2G035140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	743, 1426
AccB1I	GGYRCC	2 cut(s)	565, 663
AccII	CGCG	1 cut(s)	52
AciI	CCGC	3 cut(s)	144, 2020, 2076
AclI	AACGTT	1 cut(s)	2009
AclWI	GGATC	6 cut(s)	86, 134, 488, 501, 1451, 1508
AcsI	RAATTY	2 cut(s)	587, 706
AcuI	CTGAAG	2 cut(s)	1377, 1548
AcyI	GRCGYC	1 cut(s)	155
AfaI	GTAC	3 cut(s)	616, 1816, 2026
AfiI	CCNNNNNNNGG	5 cut(s)	33, 67, 241, 927, 1582
AjnI	CCWGG	3 cut(s)	286, 379, 921
AjuI	GAANNNNNNNTTGG	2 cut(s)	368, 400
AluBI	AGCT	7 cut(s)	332, 354, 647, 999, 1325, 1372, 2064
AluI	AGCT	7 cut(s)	332, 354, 647, 999, 1325, 1372, 2064
Alw21I	GWGCWC	1 cut(s)	1711
Alw26I	GTCTC	7 cut(s)	177, 519, 1011, 1048, 1150, 1306, 1331
Alw44I	GTGCAC	1 cut(s)	1707
AlwI	GGATC	6 cut(s)	86, 134, 488, 501, 1451, 1508
Ama87I	CYCGRG	1 cut(s)	1149
AoxI	GGCC	4 cut(s)	250, 1099, 1181, 1573
ApaLI	GTGCAC	1 cut(s)	1707
ApeKI	GCWGC	3 cut(s)	81, 98, 1369
ApoI	RAATTY	2 cut(s)	587, 706
Asp700I	GAANNNNTTC	1 cut(s)	1641
AspLEI	GCGC	1 cut(s)	39
AspS9I	GGNCC	5 cut(s)	472, 641, 1099, 1181, 1574
AsuHPI	GGTGA	4 cut(s)	10, 145, 1580, 1686
AsuNHI	GCTAGC	1 cut(s)	2078
AvaI	CYCGRG	1 cut(s)	1149
AvaII	GGWCC	2 cut(s)	472, 641
BaeGI	GKGCMC	1 cut(s)	1711
BamHI	GGATCC	1 cut(s)	493
BanI	GGYRCC	2 cut(s)	565, 663
BanII	GRGCYC	1 cut(s)	2166
BarI	GAAGNNNNNNTAC	2 cut(s)	1201, 1233
BauI	CACGAG	1 cut(s)	65
Bbv12I	GWGCWC	1 cut(s)	1711
BbvI	GCAGC	3 cut(s)	68, 85, 1381
BccI	CCATC	4 cut(s)	505, 1396, 1565, 1968
BceAI	ACGGC	3 cut(s)	50, 183, 2130
BciT130I	CCWGG	3 cut(s)	288, 381, 923
BciVI	GTATCC	1 cut(s)	1117
BclI	TGATCA	2 cut(s)	837, 948
BcoDI	GTCTC	7 cut(s)	177, 519, 1011, 1048, 1150, 1306, 1331
BfaI	CTAG	2 cut(s)	351, 2079
BfmI	CTRYAG	1 cut(s)	1125
BfoI	RGCGCY	1 cut(s)	40
BfuAI	ACCTGC	2 cut(s)	743, 1426
BfuI	GTATCC	1 cut(s)	1117
BglI	GCCNNNNNGGC	1 cut(s)	152
BglII	AGATCT	1 cut(s)	2089
BisI	GCNGC	3 cut(s)	82, 99, 1370
BlsI	GCNGC	3 cut(s)	83, 100, 1371
BmcAI	AGTACT	1 cut(s)	2026
Bme1390I	CCNGG	3 cut(s)	288, 381, 923
Bme18I	GGWCC	2 cut(s)	472, 641
BmeT110I	CYCGRG	1 cut(s)	1149
BmgT120I	GGNCC	5 cut(s)	472, 641, 1099, 1181, 1574
BmiI	GGNNCC	7 cut(s)	495, 567, 642, 665, 1363, 1908, 1929
BmrFI	CCNGG	3 cut(s)	288, 381, 923
BmrI	ACTGGG	2 cut(s)	1835, 1934
BmsI	GCATC	4 cut(s)	1430, 1678, 1700, 1764
BmtI	GCTAGC	1 cut(s)	2082
BmuI	ACTGGG	2 cut(s)	1835, 1934
BpmI	CTGGAG	3 cut(s)	309, 317, 1902
Bpu10I	CCTNAGC	1 cut(s)	2160
BpuEI	CTTGAG	1 cut(s)	516
Bsa29I	ATCGAT	1 cut(s)	1271
BsaHI	GRCGYC	1 cut(s)	155
BsaI	GGTCTC	2 cut(s)	177, 1331
BsaJI	CCNNGG	5 cut(s)	102, 402, 921, 922, 2000
BsaWI	WCCGGW	1 cut(s)	110
Bsc4I	CCNNNNNNNGG	5 cut(s)	33, 67, 241, 927, 1582
Bse1I	ACTGG	5 cut(s)	1841, 1848, 1919, 1940, 2032
BseBI	CCWGG	3 cut(s)	288, 381, 923
BseCI	ATCGAT	1 cut(s)	1271
BseDI	CCNNGG	5 cut(s)	102, 402, 921, 922, 2000
BseGI	GGATG	4 cut(s)	440, 936, 1516, 1558
BseLI	CCNNNNNNNGG	5 cut(s)	33, 67, 241, 927, 1582
BseMII	CTCAG	6 cut(s)	414, 575, 1155, 1371, 1572, 2151
BseNI	ACTGG	5 cut(s)	1841, 1848, 1919, 1940, 2032
BseRI	GAGGAG	2 cut(s)	1796, 2256
BseSI	GKGCMC	1 cut(s)	1711
BseXI	GCAGC	3 cut(s)	68, 85, 1381
Bsh1236I	CGCG	1 cut(s)	52
BshFI	GGCC	4 cut(s)	252, 1101, 1183, 1575
BshNI	GGYRCC	2 cut(s)	565, 663
BshVI	ATCGAT	1 cut(s)	1271
BsiHKAI	GWGCWC	1 cut(s)	1711
BsiHKCI	CYCGRG	1 cut(s)	1149
BsiSI	CCGG	3 cut(s)	95, 111, 147
BslFI	GGGAC	1 cut(s)	654
BslI	CCNNNNNNNGG	5 cut(s)	33, 67, 241, 927, 1582
BsmAI	GTCTC	7 cut(s)	177, 519, 1011, 1048, 1150, 1306, 1331
BsmFI	GGGAC	1 cut(s)	654
BsnI	GGCC	4 cut(s)	252, 1101, 1183, 1575
Bso31I	GGTCTC	2 cut(s)	177, 1331
BsoBI	CYCGRG	1 cut(s)	1149
Bsp1286I	GDGCHC	2 cut(s)	1711, 2166
Bsp143I	GATC	9 cut(s)	75, 91, 126, 493, 837, 948, 1456, 1513, 2089
Bsp68I	TCGCGA	1 cut(s)	52
BspACI	CCGC	3 cut(s)	144, 2020, 2076
BspANI	GGCC	4 cut(s)	252, 1101, 1183, 1575
BspCNI	CTCAG	6 cut(s)	415, 574, 1156, 1372, 1573, 2152
BspDI	ATCGAT	1 cut(s)	1271
BspFNI	CGCG	1 cut(s)	52
BspHI	TCATGA	1 cut(s)	1645
BspLI	GGNNCC	7 cut(s)	495, 567, 642, 665, 1363, 1908, 1929
BspMI	ACCTGC	2 cut(s)	743, 1426
BspOI	GCTAGC	1 cut(s)	2082
BspPI	GGATC	6 cut(s)	86, 134, 488, 501, 1451, 1508
BspQI	GCTCTTC	2 cut(s)	770, 1018
BspT107I	GGYRCC	2 cut(s)	565, 663
BspTNI	GGTCTC	2 cut(s)	177, 1331
BsrI	ACTGG	5 cut(s)	1841, 1848, 1919, 1940, 2032
BssECI	CCNNGG	5 cut(s)	102, 402, 921, 922, 2000
BssMI	GATC	9 cut(s)	75, 91, 126, 493, 837, 948, 1456, 1513, 2089
BssNI	GRCGYC	1 cut(s)	155
BssSI	CACGAG	1 cut(s)	65
BssT1I	CCWWGG	1 cut(s)	2000
Bst2BI	CACGAG	1 cut(s)	65
Bst2UI	CCWGG	3 cut(s)	288, 381, 923
Bst4CI	ACNGT	6 cut(s)	164, 316, 600, 966, 1042, 1144
Bst6I	CTCTTC	3 cut(s)	770, 1018, 1329
BstACI	GRCGYC	1 cut(s)	155
BstAPI	GCANNNNNTGC	1 cut(s)	786
BstC8I	GCNNGC	5 cut(s)	144, 196, 1467, 1524, 2080
BstDEI	CTNAG	8 cut(s)	423, 561, 748, 1164, 1380, 1581, 2060, 2160
BstF5I	GGATG	4 cut(s)	440, 936, 1516, 1558
BstFNI	CGCG	1 cut(s)	52
BstH2I	RGCGCY	1 cut(s)	40
BstHHI	GCGC	1 cut(s)	39
BstKTI	GATC	9 cut(s)	78, 94, 129, 496, 840, 951, 1459, 1516, 2092
BstMAI	GTCTC	7 cut(s)	177, 519, 1011, 1048, 1150, 1306, 1331
BstMBI	GATC	9 cut(s)	75, 91, 126, 493, 837, 948, 1456, 1513, 2089
BstMWI	GCNNNNNNNGC	6 cut(s)	95, 104, 152, 395, 786, 1844
BstNI	CCWGG	3 cut(s)	288, 381, 923
BstNSI	RCATGY	2 cut(s)	1469, 1526
BstSCI	CCNGG	3 cut(s)	286, 379, 921
BstSFI	CTRYAG	1 cut(s)	1125
BstSLI	GKGCMC	1 cut(s)	1711
BstUI	CGCG	1 cut(s)	52
BstV1I	GCAGC	3 cut(s)	68, 85, 1381
BstX2I	RGATCY	4 cut(s)	91, 126, 493, 2089
BstYI	RGATCY	4 cut(s)	91, 126, 493, 2089
Bsu15I	ATCGAT	1 cut(s)	1271
BsuI	GTATCC	1 cut(s)	1117
BsuRI	GGCC	4 cut(s)	252, 1101, 1183, 1575
BsuTUI	ATCGAT	1 cut(s)	1271
BtgZI	GCGATG	1 cut(s)	33
BtsCI	GGATG	4 cut(s)	440, 936, 1516, 1558
BtsI	GCAGTG	1 cut(s)	861
BtsIMutI	CAGTG	4 cut(s)	312, 861, 1711, 2211
BtuMI	TCGCGA	1 cut(s)	52
BveI	ACCTGC	2 cut(s)	743, 1426
Cac8I	GCNNGC	5 cut(s)	144, 196, 1467, 1524, 2080
CciI	TCATGA	1 cut(s)	1645
CfoI	GCGC	1 cut(s)	39
Cfr13I	GGNCC	5 cut(s)	472, 641, 1099, 1181, 1574
ClaI	ATCGAT	1 cut(s)	1271
CseI	GACGC	1 cut(s)	144
CsiI	ACCWGGT	1 cut(s)	379
Csp6I	GTAC	3 cut(s)	615, 1815, 2025
CspCI	CAANNNNNGTGG	2 cut(s)	301, 336
CviQI	GTAC	3 cut(s)	615, 1815, 2025
DdeI	CTNAG	8 cut(s)	423, 561, 748, 1164, 1380, 1581, 2060, 2160
DpnI	GATC	9 cut(s)	77, 93, 128, 495, 839, 950, 1458, 1515, 2091
DpnII	GATC	9 cut(s)	75, 91, 126, 493, 837, 948, 1456, 1513, 2089
DraI	TTTAAA	1 cut(s)	2070
Eam1104I	CTCTTC	3 cut(s)	770, 1018, 1329
EarI	CTCTTC	3 cut(s)	770, 1018, 1329
Eco130I	CCWWGG	1 cut(s)	2000
Eco24I	GRGCYC	1 cut(s)	2166
Eco31I	GGTCTC	2 cut(s)	177, 1331
Eco47I	GGWCC	2 cut(s)	472, 641
Eco57I	CTGAAG	2 cut(s)	1377, 1548
Eco88I	CYCGRG	1 cut(s)	1149
EcoRII	CCWGG	3 cut(s)	286, 379, 921
EcoT14I	CCWWGG	1 cut(s)	2000
EcoT22I	ATGCAT	2 cut(s)	1624, 1693
EcoT38I	GRGCYC	1 cut(s)	2166
ErhI	CCWWGG	1 cut(s)	2000
FalI	AAGNNNNNCTT	6 cut(s)	338, 370, 722, 754, 2043, 2075
FaqI	GGGAC	1 cut(s)	654
FauI	CCCGC	1 cut(s)	151
FauNDI	CATATG	1 cut(s)	457
FbaI	TGATCA	2 cut(s)	837, 948
Fnu4HI	GCNGC	3 cut(s)	82, 99, 1370
FokI	GGATG	4 cut(s)	447, 943, 1523, 1565
FriOI	GRGCYC	1 cut(s)	2166
Fsp4HI	GCNGC	3 cut(s)	82, 99, 1370
FspBI	CTAG	2 cut(s)	351, 2079
GlaI	GCGC	1 cut(s)	38
GluI	GCNGC	3 cut(s)	82, 99, 1370
GsuI	CTGGAG	3 cut(s)	309, 317, 1902
HaeII	RGCGCY	1 cut(s)	40
HaeIII	GGCC	4 cut(s)	252, 1101, 1183, 1575
HapII	CCGG	3 cut(s)	95, 111, 147
HgaI	GACGC	1 cut(s)	144
HhaI	GCGC	1 cut(s)	39
Hin1I	GRCGYC	1 cut(s)	155
Hin6I	GCGC	1 cut(s)	37
HinP1I	GCGC	1 cut(s)	37
HincII	GTYRAC	2 cut(s)	1894, 1993
HindII	GTYRAC	2 cut(s)	1894, 1993
HindIII	AAGCTT	1 cut(s)	997
HinfI	GANTC	9 cut(s)	63, 206, 917, 980, 1384, 1405, 1829, 1915, 2173
HpaII	CCGG	3 cut(s)	95, 111, 147
HphI	GGTGA	4 cut(s)	10, 145, 1580, 1686
Hpy166II	GTNNAC	5 cut(s)	136, 626, 1709, 1894, 1993
Hpy8I	GTNNAC	5 cut(s)	136, 626, 1709, 1894, 1993
Hpy99I	CGWCG	1 cut(s)	160
HpyAV	CCTTC	9 cut(s)	728, 765, 1090, 1227, 1270, 1352, 1477, 1651, 2176
HpyCH4III	ACNGT	6 cut(s)	164, 316, 600, 966, 1042, 1144
HpyCH4IV	ACGT	2 cut(s)	15, 2009
HpyF10VI	GCNNNNNNNGC	6 cut(s)	95, 104, 152, 395, 786, 1844
HpyF3I	CTNAG	8 cut(s)	423, 561, 748, 1164, 1380, 1581, 2060, 2160
HpySE526I	ACGT	2 cut(s)	15, 2009
Hsp92I	GRCGYC	1 cut(s)	155
HspAI	GCGC	1 cut(s)	37
Ksp22I	TGATCA	2 cut(s)	837, 948
Kzo9I	GATC	9 cut(s)	75, 91, 126, 493, 837, 948, 1456, 1513, 2089
LguI	GCTCTTC	2 cut(s)	770, 1018
LmnI	GCTCC	3 cut(s)	44, 329, 1495
Lsp1109I	GCAGC	3 cut(s)	68, 85, 1381
LweI	GCATC	4 cut(s)	1430, 1678, 1700, 1764
MabI	ACCWGGT	1 cut(s)	379
MaeI	CTAG	2 cut(s)	351, 2079
MaeII	ACGT	2 cut(s)	15, 2009
MaeIII	GTNAC	4 cut(s)	16, 166, 1036, 1598
MalI	GATC	9 cut(s)	77, 93, 128, 495, 839, 950, 1458, 1515, 2091
MboI	GATC	9 cut(s)	75, 91, 126, 493, 837, 948, 1456, 1513, 2089
MflI	RGATCY	4 cut(s)	91, 126, 493, 2089
MhlI	GDGCHC	2 cut(s)	1711, 2166
MlyI	GAGTC	5 cut(s)	57, 215, 911, 1909, 2182
MmeI	TCCRAC	4 cut(s)	33, 66, 85, 138
Mph1103I	ATGCAT	2 cut(s)	1624, 1693
MroXI	GAANNNNTTC	1 cut(s)	1641
MslI	CAYNNNNRTG	2 cut(s)	480, 1772
MspA1I	CMGCKG	1 cut(s)	647
MspI	CCGG	3 cut(s)	95, 111, 147
MspR9I	CCNGG	3 cut(s)	288, 381, 923
MvaI	CCWGG	3 cut(s)	288, 381, 923
MvnI	CGCG	1 cut(s)	52
MwoI	GCNNNNNNNGC	6 cut(s)	95, 104, 152, 395, 786, 1844
NdeI	CATATG	1 cut(s)	457
NdeII	GATC	9 cut(s)	75, 91, 126, 493, 837, 948, 1456, 1513, 2089
NheI	GCTAGC	1 cut(s)	2078
NlaIV	GGNNCC	7 cut(s)	495, 567, 642, 665, 1363, 1908, 1929
NmeAIII	GCCGAG	1 cut(s)	127
NmuCI	GTSAC	4 cut(s)	16, 166, 1036, 1598
NruI	TCGCGA	1 cut(s)	52
NsiI	ATGCAT	2 cut(s)	1624, 1693
NspI	RCATGY	2 cut(s)	1469, 1526
PaeI	GCATGC	2 cut(s)	1469, 1526
PagI	TCATGA	1 cut(s)	1645
PasI	CCCWGGG	1 cut(s)	922
PciSI	GCTCTTC	2 cut(s)	770, 1018
PdmI	GAANNNNTTC	1 cut(s)	1641
PfeI	GAWTC	4 cut(s)	980, 1384, 1405, 1829
PflFI	GACNNNGTC	1 cut(s)	164
PfoI	TCCNGGA	1 cut(s)	286
PkrI	GCNGC	3 cut(s)	83, 100, 1371
PleI	GAGTC	5 cut(s)	57, 214, 911, 1909, 2181
PpsI	GAGTC	5 cut(s)	57, 214, 911, 1909, 2181
Psp1406I	AACGTT	1 cut(s)	2009
Psp6I	CCWGG	3 cut(s)	286, 379, 921
PspGI	CCWGG	3 cut(s)	286, 379, 921
PspN4I	GGNNCC	7 cut(s)	495, 567, 642, 665, 1363, 1908, 1929
PspPI	GGNCC	5 cut(s)	472, 641, 1099, 1181, 1574
PsuI	RGATCY	4 cut(s)	91, 126, 493, 2089
PsyI	GACNNNGTC	1 cut(s)	164
PvuII	CAGCTG	1 cut(s)	647
RruI	TCGCGA	1 cut(s)	52
RsaI	GTAC	3 cut(s)	616, 1816, 2026
RsaNI	GTAC	3 cut(s)	615, 1815, 2025
RseI	CAYNNNNRTG	2 cut(s)	480, 1772
SapI	GCTCTTC	2 cut(s)	770, 1018
SatI	GCNGC	3 cut(s)	82, 99, 1370
Sau3AI	GATC	9 cut(s)	75, 91, 126, 493, 837, 948, 1456, 1513, 2089
Sau96I	GGNCC	5 cut(s)	472, 641, 1099, 1181, 1574
ScaI	AGTACT	1 cut(s)	2026
SchI	GAGTC	5 cut(s)	57, 215, 911, 1909, 2182
ScrFI	CCNGG	3 cut(s)	288, 381, 923
SduI	GDGCHC	2 cut(s)	1711, 2166
SexAI	ACCWGGT	1 cut(s)	379
SfaNI	GCATC	4 cut(s)	1430, 1678, 1700, 1764
SfcI	CTRYAG	1 cut(s)	1125
SinI	GGWCC	2 cut(s)	472, 641
SmiMI	CAYNNNNRTG	2 cut(s)	480, 1772
SmlI	CTYRAG	1 cut(s)	531
SmoI	CTYRAG	1 cut(s)	531
SphI	GCATGC	2 cut(s)	1469, 1526
SsiI	CCGC	3 cut(s)	144, 2020, 2076
SspMI	CTAG	2 cut(s)	351, 2079
StyD4I	CCNGG	3 cut(s)	286, 379, 921
StyI	CCWWGG	1 cut(s)	2000
TaaI	ACNGT	6 cut(s)	164, 316, 600, 966, 1042, 1144
TaiI	ACGT	2 cut(s)	18, 2012
TaqI	TCGA	3 cut(s)	368, 1271, 2253
TatI	WGTACW	2 cut(s)	1814, 2024
TfiI	GAWTC	4 cut(s)	980, 1384, 1405, 1829
TscAI	CASTG	4 cut(s)	319, 861, 1711, 2211
TseFI	GTSAC	4 cut(s)	16, 166, 1036, 1598
TseI	GCWGC	3 cut(s)	81, 98, 1369
Tsp45I	GTSAC	4 cut(s)	16, 166, 1036, 1598
TspDTI	ATGAA	9 cut(s)	228, 774, 1077, 1263, 1289, 1634, 1751, 1785, 2243
TspGWI	ACGGA	1 cut(s)	447
TspRI	CASTG	4 cut(s)	319, 861, 1711, 2211
Tth111I	GACNNNGTC	1 cut(s)	164
VneI	GTGCAC	1 cut(s)	1707
VpaK11BI	GGWCC	2 cut(s)	472, 641
XapI	RAATTY	2 cut(s)	587, 706
XceI	RCATGY	2 cut(s)	1469, 1526
XmnI	GAANNNNTTC	1 cut(s)	1641
XspI	CTAG	2 cut(s)	351, 2079
ZrmI	AGTACT	1 cut(s)	2026
Zsp2I	ATGCAT	2 cut(s)	1624, 1693

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.