RLG00000027535

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006950
Physical Location & Seq
Reverse (-)
12482850 .. 12483340
491 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027535

Sequence Viewer

Length: 405 bp
ATGGAGACTAATAAGTTCAGTTTGGTGGTGGTAGTGCTCACCTTTTTCACTCCAACTGCCTTCGCCGTGTGGCATACAAATTATGAAGTAGCCTCGGCAGCTGCAGAGCCTGCAGAATCTTATGATCATATTGAAGTAGCCTTGGCCGCGGCTAAGCCTGACCTTGACGAGAGTATTGAAATAGCCTCGGCCGCTGCTGAGCCTGCGGATCCTGTGGATTATTATGATAATATTATGCCCTTGCAAGATGGGTTGTATTTGCGTTCCGTTTGGAATGCTTCCGATTGGGCTCTGCATTGGTCTCTGTCATTGCAATATTACTCCACCTCCAGGTCCACAGCCACAGCCGCCTTCAGTCTCACTATCACCGTCACCATCACCGTCACCACTGGTGTTTTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.58

Weight (kDa)

4.15

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 149
AciI CCGC 5 cut(s) 147, 149, 192, 206, 348
AclWI GGATC 2 cut(s) 203, 216
AcoI YGGCCR 2 cut(s) 144, 189
AcuI CTGAAG 1 cut(s) 337
AfiI CCNNNNNNNGG 1 cut(s) 330
AgsI TTSAA 2 cut(s) 134, 179
AjnI CCWGG 1 cut(s) 329
AluBI AGCT 1 cut(s) 101
AluI AGCT 1 cut(s) 101
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 2 cut(s) 306, 362
AlwI GGATC 2 cut(s) 203, 216
AlwNI CAGNNNCTG 1 cut(s) 110
AoxI GGCC 2 cut(s) 144, 189
ApeKI GCWGC 3 cut(s) 98, 101, 194
AspS9I GGNCC 1 cut(s) 333
AsuHPI GGTGA 5 cut(s) 31, 358, 364, 370, 376
AvaII GGWCC 1 cut(s) 333
BamHI GGATCC 1 cut(s) 208
BanII GRGCYC 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 88, 110, 181
BccI CCATC 2 cut(s) 242, 383
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 331
BclI TGATCA 1 cut(s) 124
BcoDI GTCTC 2 cut(s) 306, 362
BfmI CTRYAG 2 cut(s) 102, 111
BisI GCNGC 7 cut(s) 99, 102, 147, 150, 192, 195, 348
BlpI GCTNAGC 2 cut(s) 153, 198
BlsI GCNGC 7 cut(s) 100, 103, 148, 151, 193, 196, 349
Bme1390I CCNGG 1 cut(s) 331
Bme18I GGWCC 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 1 cut(s) 210
BmrFI CCNGG 1 cut(s) 331
BpmI CTGGAG 1 cut(s) 313
Bpu1102I GCTNAGC 2 cut(s) 153, 198
BsaI GGTCTC 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 93, 141, 147, 186
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 330
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 308
BseBI CCWGG 1 cut(s) 331
BseDI CCNNGG 4 cut(s) 93, 141, 147, 186
BseLI CCNNNNNNNGG 1 cut(s) 330
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 394
BseX3I CGGCCG 1 cut(s) 189
BseXI GCAGC 3 cut(s) 88, 110, 181
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 2 cut(s) 146, 191
BsiEI CGRYCG 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 330
BsmAI GTCTC 2 cut(s) 306, 362
BsmI GAATGC 1 cut(s) 280
BsnI GGCC 2 cut(s) 146, 191
Bso31I GGTCTC 1 cut(s) 306
Bsp1286I GDGCHC 2 cut(s) 39, 292
Bsp143I GATC 2 cut(s) 124, 208
Bsp1720I GCTNAGC 2 cut(s) 153, 198
BspACI CCGC 5 cut(s) 147, 149, 192, 206, 348
BspANI GGCC 2 cut(s) 146, 191
BspCNI CTCAG 1 cut(s) 190
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 1 cut(s) 210
BspMAI CTGCAG 2 cut(s) 106, 115
BspPI GGATC 2 cut(s) 203, 216
BspTNI GGTCTC 1 cut(s) 306
BsrDI GCAATG 1 cut(s) 308
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 4 cut(s) 93, 141, 147, 186
BssMI GATC 2 cut(s) 124, 208
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 2 cut(s) 370, 382
BstAPI GCANNNNNTGC 1 cut(s) 110
BstC8I GCNNGC 2 cut(s) 111, 204
BstDEI CTNAG 2 cut(s) 153, 198
BstDSI CCRYGG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 149
BstKTI GATC 2 cut(s) 127, 211
BstMAI GTCTC 2 cut(s) 306, 362
BstMBI GATC 2 cut(s) 124, 208
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 6 cut(s) 98, 110, 146, 191, 203, 347
BstNI CCWGG 1 cut(s) 331
BstSCI CCNGG 1 cut(s) 329
BstSFI CTRYAG 2 cut(s) 102, 111
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 3 cut(s) 88, 110, 181
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BstZI CGGCCG 1 cut(s) 189
BsuRI GGCC 2 cut(s) 146, 191
BtgI CCRYGG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 387
Cac8I GCNNGC 2 cut(s) 111, 204
CaiI CAGNNNCTG 1 cut(s) 110
Cfr13I GGNCC 1 cut(s) 333
Cfr42I CCGCGG 1 cut(s) 150
DdeI CTNAG 2 cut(s) 153, 198
DpnI GATC 2 cut(s) 126, 210
DpnII GATC 2 cut(s) 124, 208
EaeI YGGCCR 2 cut(s) 144, 189
EagI CGGCCG 1 cut(s) 189
EclXI CGGCCG 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 333
Eco52I CGGCCG 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 141
FaiI YATR 7 cut(s) 75, 84, 123, 129, 225, 236, 403
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 7 cut(s) 99, 102, 147, 150, 192, 195, 348
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 7 cut(s) 99, 102, 147, 150, 192, 195, 348
GluI GCNGC 7 cut(s) 99, 102, 147, 150, 192, 195, 348
GsuI CTGGAG 1 cut(s) 313
HaeIII GGCC 2 cut(s) 146, 191
HinfI GANTC 1 cut(s) 116
HphI GGTGA 5 cut(s) 31, 358, 364, 370, 376
Hpy166II GTNNAC 1 cut(s) 336
Hpy188I TCNGA 1 cut(s) 283
Hpy8I GTNNAC 1 cut(s) 336
HpyAV CCTTC 2 cut(s) 70, 361
HpyCH4III ACNGT 2 cut(s) 370, 382
HpyCH4V TGCA 5 cut(s) 104, 113, 244, 295, 313
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 110, 146, 191, 203, 347
HpyF3I CTNAG 2 cut(s) 153, 198
Ksp22I TGATCA 1 cut(s) 124
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 2 cut(s) 124, 208
LpnPI CCDG 7 cut(s) 123, 171, 216, 225, 316, 343, 375
Lsp1109I GCAGC 3 cut(s) 88, 110, 181
MaeIII GTNAC 2 cut(s) 370, 382
MalI GATC 2 cut(s) 126, 210
MboI GATC 2 cut(s) 124, 208
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 2 cut(s) 39, 292
MluCI AATT 1 cut(s) 79
MmeI TCCRAC 1 cut(s) 77
MnlI CCTC 3 cut(s) 103, 196, 337
MspA1I CMGCKG 3 cut(s) 101, 149, 194
MspR9I CCNGG 1 cut(s) 331
Mva1269I GAATGC 1 cut(s) 280
MvaI CCWGG 1 cut(s) 331
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 6 cut(s) 98, 110, 146, 191, 203, 347
NdeII GATC 2 cut(s) 124, 208
NlaIV GGNNCC 1 cut(s) 210
NmeAIII GCCGAG 2 cut(s) 74, 167
NmuCI GTSAC 2 cut(s) 370, 382
PctI GAATGC 1 cut(s) 280
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 7 cut(s) 100, 103, 148, 151, 193, 196, 349
Psp6I CCWGG 1 cut(s) 329
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 1 cut(s) 333
PstI CTGCAG 2 cut(s) 106, 115
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 101
SacII CCGCGG 1 cut(s) 150
SatI GCNGC 7 cut(s) 99, 102, 147, 150, 192, 195, 348
Sau3AI GATC 2 cut(s) 124, 208
Sau96I GGNCC 1 cut(s) 333
ScrFI CCNGG 1 cut(s) 331
SduI GDGCHC 2 cut(s) 39, 292
SetI ASST 5 cut(s) 44, 103, 165, 329, 335
SfcI CTRYAG 2 cut(s) 102, 111
Sfr303I CCGCGG 1 cut(s) 150
SgrBI CCGCGG 1 cut(s) 150
SinI GGWCC 1 cut(s) 333
Sse9I AATT 1 cut(s) 79
SsiI CCGC 5 cut(s) 147, 149, 192, 206, 348
SspI AATATT 2 cut(s) 232, 317
StyD4I CCNGG 1 cut(s) 329
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 370, 382
TasI AATT 1 cut(s) 79
TauI GCSGC 4 cut(s) 149, 152, 194, 350
TfiI GAWTC 1 cut(s) 116
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 2 cut(s) 370, 382
TseI GCWGC 3 cut(s) 98, 101, 194
Tsp45I GTSAC 2 cut(s) 370, 382
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.