Rroxscaffold_4G00292860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006950
Physical Location & Seq
Reverse (-)
13059849 .. 13060253
405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00292860.1

Sequence Viewer

Length: 405 bp
ATGGTTCTCGTGTCCCCCTTTTTCAATCCAACTGCTTTCGCCGTGCGGCATAAGAATTATGAAGTAGTCTCGGCCGCTGCGGAGCCTGATTCTAACGATCGTAACGAAGTAGCCTCGGCCGATGCTGAGCCGGGTCCTGTTGATTATTATGATAATATCATGGTAAAAGACGAAAGCAGCAGAAATACTGGATCAAAACGTCTGAAGGTCGACGACGGTTGTCCATCGTCGGTATGCGATATGACAAAACCATGCAAGATTGGCTGTATTTGCGTTCCCTTTGTTCCGTTTCCTATTGGAACCTGCGTTGGTATCTGTTGTCAAATTAATCCACCTCCAGGTGCACCGCCTCTACCACCATCATCACCGTCACCATCGCCGTCACTGTCACCAGTGAAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

134

Amino Acids

14.19

Weight (kDa)

4.98

Isoelectric Point (pI)

67.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 311
AccI GTMKAC 1 cut(s) 210
AciI CCGC 4 cut(s) 46, 75, 80, 347
AclWI GGATC 1 cut(s) 199
AcoI YGGCCR 2 cut(s) 72, 117
AcsI RAATTY 1 cut(s) 398
AcuI CTGAAG 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 338
AgsI TTSAA 1 cut(s) 25
AjnI CCWGG 1 cut(s) 337
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 1 cut(s) 73
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 1 cut(s) 199
AoxI GGCC 2 cut(s) 72, 117
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 77, 177
ApoI RAATTY 1 cut(s) 398
AseI ATTAAT 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 134
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 3 cut(s) 357, 363, 381
AvaII GGWCC 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 346
BauI CACGAG 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 64, 189
BccI CCATC 3 cut(s) 232, 367, 382
BceAI ACGGC 2 cut(s) 26, 364
BciT130I CCWGG 1 cut(s) 339
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 311
BisI GCNGC 4 cut(s) 47, 75, 78, 178
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 4 cut(s) 48, 76, 79, 179
Bme1390I CCNGG 2 cut(s) 132, 339
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 3 cut(s) 84, 135, 301
BmrFI CCNGG 2 cut(s) 132, 339
BmsI GCATC 1 cut(s) 112
BoxI GACNNNNGTC 1 cut(s) 219
BpmI CTGGAG 1 cut(s) 321
Bpu1102I GCTNAGC 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 114
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 2 cut(s) 193, 392
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 2 cut(s) 193, 392
BseSI GKGCMC 1 cut(s) 346
BseX3I CGGCCG 2 cut(s) 72, 117
BseXI GCAGC 2 cut(s) 64, 189
Bsh1285I CGRYCG 3 cut(s) 75, 100, 120
BshFI GGCC 2 cut(s) 74, 119
BsiEI CGRYCG 3 cut(s) 75, 100, 120
BsiHKAI GWGCWC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 2 cut(s) 74, 119
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 2 cut(s) 97, 191
Bsp1720I GCTNAGC 1 cut(s) 126
BspACI CCGC 4 cut(s) 46, 75, 80, 347
BspANI GGCC 2 cut(s) 74, 119
BspCNI CTCAG 1 cut(s) 118
BspLI GGNNCC 3 cut(s) 84, 135, 301
BspMI ACCTGC 1 cut(s) 311
BspPI GGATC 1 cut(s) 199
BsrI ACTGG 2 cut(s) 193, 392
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 2 cut(s) 97, 191
BssSI CACGAG 1 cut(s) 8
Bst2BI CACGAG 1 cut(s) 8
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 3 cut(s) 218, 369, 387
BstDEI CTNAG 1 cut(s) 126
BstKTI GATC 2 cut(s) 100, 194
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 2 cut(s) 97, 191
BstMCI CGRYCG 3 cut(s) 75, 100, 120
BstMWI GCNNNNNNNGC 2 cut(s) 261, 270
BstNI CCWGG 1 cut(s) 339
BstPAI GACNNNNGTC 1 cut(s) 219
BstSCI CCNGG 2 cut(s) 130, 337
BstSLI GKGCMC 1 cut(s) 346
BstV1I GCAGC 2 cut(s) 64, 189
BstZI CGGCCG 2 cut(s) 72, 117
BsuRI GGCC 2 cut(s) 74, 119
BtgZI GCGATG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 383, 399
BveI ACCTGC 1 cut(s) 311
Cfr13I GGNCC 1 cut(s) 134
CviAII CATG 2 cut(s) 160, 252
CviJI RGCY 6 cut(s) 74, 85, 113, 119, 130, 264
CviKI_1 RGCY 6 cut(s) 74, 85, 113, 119, 130, 264
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 99, 193
DpnII GATC 2 cut(s) 97, 191
EaeI YGGCCR 2 cut(s) 72, 117
EagI CGGCCG 2 cut(s) 72, 117
EclXI CGGCCG 2 cut(s) 72, 117
Eco47I GGWCC 1 cut(s) 134
Eco52I CGGCCG 2 cut(s) 72, 117
Eco57I CTGAAG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 337
FaeI CATG 2 cut(s) 163, 255
FaiI YATR 7 cut(s) 51, 60, 150, 161, 235, 242, 253
FatI CATG 2 cut(s) 159, 251
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 4 cut(s) 47, 75, 78, 178
Fsp4HI GCNGC 4 cut(s) 47, 75, 78, 178
GluI GCNGC 4 cut(s) 47, 75, 78, 178
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 2 cut(s) 74, 119
HapII CCGG 1 cut(s) 131
Hin1II CATG 2 cut(s) 163, 255
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 1 cut(s) 89
HpaII CCGG 1 cut(s) 131
HphI GGTGA 3 cut(s) 357, 363, 381
Hpy166II GTNNAC 2 cut(s) 211, 344
Hpy188I TCNGA 1 cut(s) 204
Hpy8I GTNNAC 2 cut(s) 211, 344
Hpy99I CGWCG 3 cut(s) 215, 218, 232
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 3 cut(s) 218, 369, 387
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 2 cut(s) 255, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 270
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 199
Hsp92II CATG 2 cut(s) 163, 255
Kzo9I GATC 2 cut(s) 97, 191
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 7 cut(s) 99, 144, 150, 174, 316, 324, 351
Lsp1109I GCAGC 2 cut(s) 64, 189
LweI GCATC 1 cut(s) 112
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 4 cut(s) 101, 369, 381, 387
MalI GATC 2 cut(s) 99, 193
MboI GATC 2 cut(s) 97, 191
MhlI GDGCHC 1 cut(s) 346
MluCI AATT 3 cut(s) 55, 324, 398
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 3 cut(s) 124, 345, 360
MseI TTAA 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 2 cut(s) 132, 339
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 261, 270
NciI CCSGG 1 cut(s) 132
NdeII GATC 2 cut(s) 97, 191
NlaIII CATG 2 cut(s) 163, 255
NlaIV GGNNCC 3 cut(s) 84, 135, 301
NmeAIII GCCGAG 2 cut(s) 50, 95
NmuCI GTSAC 3 cut(s) 369, 381, 387
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 4 cut(s) 48, 76, 79, 179
Ple19I CGATCG 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 134
PshAI GACNNNNGTC 1 cut(s) 219
PshBI ATTAAT 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 3 cut(s) 84, 135, 301
PspPI GGNCC 1 cut(s) 134
PspPPI RGGWCCY 1 cut(s) 134
PvuI CGATCG 1 cut(s) 100
SalI GTCGAC 1 cut(s) 209
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 4 cut(s) 47, 75, 78, 178
Sau3AI GATC 2 cut(s) 97, 191
Sau96I GGNCC 1 cut(s) 134
ScrFI CCNGG 2 cut(s) 132, 339
SduI GDGCHC 1 cut(s) 346
SetI ASST 5 cut(s) 202, 210, 305, 337, 343
SfaNI GCATC 1 cut(s) 112
SinI GGWCC 1 cut(s) 134
Sse9I AATT 3 cut(s) 55, 324, 398
SsiI CCGC 4 cut(s) 46, 75, 80, 347
StyD4I CCNGG 2 cut(s) 130, 337
TaaI ACNGT 3 cut(s) 218, 369, 387
TaiI ACGT 1 cut(s) 202
TaqI TCGA 1 cut(s) 210
TasI AATT 3 cut(s) 55, 324, 398
TauI GCSGC 2 cut(s) 49, 77
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 2 cut(s) 390, 399
TseFI GTSAC 3 cut(s) 369, 381, 387
TseI GCWGC 2 cut(s) 77, 177
Tsp45I GTSAC 3 cut(s) 369, 381, 387
TspDTI ATGAA 1 cut(s) 75
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 2 cut(s) 390, 399
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 134
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.