Rmu_co8431381.1_g000001

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8431381.1
OG0008890
Physical Location & Seq
Forward (+)
1 .. 670
670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8431381.1_g000001.1.cds

Sequence Viewer

Length: 542 bp
actgtactctggcccgagaaaagtatagagagcactgggaactcaatggcacaattggcggtggtcacccagaagcggggtttgaatatgcagcggactacggaatcaaccgggagatcaattatccattcatcgggcgggaccaaccatgcaacgccaaattggaaagatatccttatacaaagataagtggttacttgggcatacctccaaatgatgaaaatcttatgaagaaatttgtggctagccaaccgattgcagccgtaatggaaatcactaggacattccatcgttacatagatggtgtataccaagatgaatttggggaatgtggatggttaccaataaaacaccgtagctatcacgctgttacagttgttgggtacgggacagatgaagatgacatggactactggttagtgaagaattcttggggcccgtattggggtgaagatggatattttcgaatgcaaagaaatgtgtatcggcgaggtggtttatgtggcatcgcatcacggctttccttcccgttcattctctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04210 ko04612 ko04621 ko04924 map04140 map04142 map04210 map04612 map04621 map04924
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

179

Amino Acids

20.72

Weight (kDa)

5.97

Isoelectric Point (pI)

27.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 308
AciI CCGC 4 cut(s) 59, 76, 94, 138
AcsI RAATTY 3 cut(s) 235, 319, 426
AfaI GTAC 2 cut(s) 6, 385
AfiI CCNNNNNNNGG 5 cut(s) 75, 76, 133, 444, 445
AgsI TTSAA 1 cut(s) 85
AluBI AGCT 1 cut(s) 359
AluI AGCT 1 cut(s) 359
Alw21I GWGCWC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 2 cut(s) 11, 435
ApaI GGGCCC 1 cut(s) 439
ApeKI GCWGC 2 cut(s) 91, 259
ApoI RAATTY 3 cut(s) 235, 319, 426
AspS9I GGNCC 4 cut(s) 12, 141, 435, 436
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 2 cut(s) 58, 460
AsuII TTCGAA 1 cut(s) 465
AsuNHI GCTAGC 1 cut(s) 244
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 439
BanII GRGCYC 1 cut(s) 439
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 103, 271
BccI CCATC 4 cut(s) 295, 296, 329, 448
BceAI ACGGC 2 cut(s) 247, 532
BcnI CCSGG 1 cut(s) 112
BfaI CTAG 3 cut(s) 245, 278, 540
BisI GCNGC 2 cut(s) 92, 260
BlsI GCNGC 2 cut(s) 93, 261
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 141
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 4 cut(s) 12, 141, 435, 436
BmiI GGNNCC 3 cut(s) 142, 436, 437
BmrFI CCNGG 1 cut(s) 112
BmrI ACTGGG 1 cut(s) 45
BmsI GCATC 2 cut(s) 515, 520
BmtI GCTAGC 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 45
Bpu14I TTCGAA 1 cut(s) 465
BpuMI CCSGG 1 cut(s) 112
BsaBI GATNNNNATC 1 cut(s) 221
Bsc4I CCNNNNNNNGG 5 cut(s) 75, 76, 133, 444, 445
Bse1I ACTGG 2 cut(s) 40, 418
Bse8I GATNNNNATC 1 cut(s) 221
BseGI GGATG 1 cut(s) 340
BseJI GATNNNNATC 1 cut(s) 221
BseLI CCNNNNNNNGG 5 cut(s) 75, 76, 133, 444, 445
BseNI ACTGG 2 cut(s) 40, 418
BseSI GKGCMC 1 cut(s) 439
BseXI GCAGC 2 cut(s) 103, 271
BshFI GGCC 2 cut(s) 13, 437
BsiHKAI GWGCWC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 2 cut(s) 154, 402
BslI CCNNNNNNNGG 5 cut(s) 75, 76, 133, 444, 445
BsmFI GGGAC 2 cut(s) 154, 402
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 2 cut(s) 13, 437
BsoBI CYCGRG 1 cut(s) 14
Bsp119I TTCGAA 1 cut(s) 465
Bsp120I GGGCCC 1 cut(s) 435
Bsp1286I GDGCHC 2 cut(s) 35, 439
Bsp143I GATC 1 cut(s) 116
BspACI CCGC 4 cut(s) 59, 76, 94, 138
BspANI GGCC 2 cut(s) 13, 437
BspLI GGNNCC 3 cut(s) 142, 436, 437
BspOI GCTAGC 1 cut(s) 248
BspT104I TTCGAA 1 cut(s) 465
BsrI ACTGG 2 cut(s) 40, 418
BssMI GATC 1 cut(s) 116
BssNAI GTATAC 1 cut(s) 309
Bst1107I GTATAC 1 cut(s) 309
Bst4CI ACNGT 3 cut(s) 4, 355, 375
BstBI TTCGAA 1 cut(s) 465
BstC8I GCNNGC 1 cut(s) 246
BstEII GGTNACC 2 cut(s) 64, 338
BstF5I GGATG 1 cut(s) 340
BstKTI GATC 1 cut(s) 119
BstMBI GATC 1 cut(s) 116
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstPI GGTNACC 2 cut(s) 64, 338
BstSCI CCNGG 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 439
BstV1I GCAGC 2 cut(s) 103, 271
BstZ17I GTATAC 1 cut(s) 309
BsuRI GGCC 2 cut(s) 13, 437
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 246
Cfr13I GGNCC 4 cut(s) 12, 141, 435, 436
Csp6I GTAC 2 cut(s) 5, 384
CviAII CATG 2 cut(s) 149, 405
CviJI RGCY 7 cut(s) 13, 244, 248, 262, 359, 437, 519
CviKI_1 RGCY 7 cut(s) 13, 244, 248, 262, 359, 437, 519
CviQI GTAC 2 cut(s) 5, 384
DpnI GATC 1 cut(s) 118
DpnII GATC 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 439
Eco32I GATATC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 14
Eco91I GGTNACC 2 cut(s) 64, 338
EcoO109I RGGNCCY 1 cut(s) 435
EcoO65I GGTNACC 2 cut(s) 64, 338
EcoRI GAATTC 1 cut(s) 426
EcoRV GATATC 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 439
FaeI CATG 2 cut(s) 152, 408
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 2 cut(s) 154, 402
FatI CATG 2 cut(s) 148, 404
FauI CCCGC 2 cut(s) 69, 131
FblI GTMKAC 1 cut(s) 308
Fnu4HI GCNGC 2 cut(s) 92, 260
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 2 cut(s) 92, 260
FspBI CTAG 3 cut(s) 245, 278, 540
GluI GCNGC 2 cut(s) 92, 260
HaeIII GGCC 2 cut(s) 13, 437
HapII CCGG 1 cut(s) 111
Hin1II CATG 2 cut(s) 152, 408
HinfI GANTC 1 cut(s) 104
HpaII CCGG 1 cut(s) 111
HphI GGTGA 2 cut(s) 58, 460
Hpy166II GTNNAC 1 cut(s) 309
Hpy8I GTNNAC 1 cut(s) 309
HpyAV CCTTC 1 cut(s) 534
HpyCH4III ACNGT 3 cut(s) 4, 355, 375
HpyCH4V TGCA 4 cut(s) 91, 152, 259, 471
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
Hsp92II CATG 2 cut(s) 152, 408
Kzo9I GATC 1 cut(s) 116
LpnPI CCDG 4 cut(s) 21, 83, 124, 399
Lsp1109I GCAGC 2 cut(s) 103, 271
LweI GCATC 2 cut(s) 515, 520
MaeI CTAG 3 cut(s) 245, 278, 540
MaeIII GTNAC 5 cut(s) 64, 193, 292, 338, 369
MalI GATC 1 cut(s) 118
MboI GATC 1 cut(s) 116
MboII GAAGA 4 cut(s) 243, 409, 435, 463
MfeI CAATTG 1 cut(s) 53
MhlI GDGCHC 2 cut(s) 35, 439
MluCI AATT 6 cut(s) 53, 120, 160, 235, 319, 426
MnlI CCTC 2 cut(s) 218, 484
MspA1I CMGCKG 1 cut(s) 94
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 1 cut(s) 112
MunI CAATTG 1 cut(s) 53
Mva1269I GAATGC 1 cut(s) 473
MwoI GCNNNNNNNGC 1 cut(s) 56
NciI CCSGG 1 cut(s) 112
NdeII GATC 1 cut(s) 116
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 2 cut(s) 152, 408
NlaIV GGNNCC 3 cut(s) 142, 436, 437
NmuCI GTSAC 1 cut(s) 64
NspV TTCGAA 1 cut(s) 465
PctI GAATGC 1 cut(s) 473
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 2 cut(s) 93, 261
PspEI GGTNACC 2 cut(s) 64, 338
PspN4I GGNNCC 3 cut(s) 142, 436, 437
PspOMI GGGCCC 1 cut(s) 435
PspPI GGNCC 4 cut(s) 12, 141, 435, 436
RsaI GTAC 2 cut(s) 6, 385
RsaNI GTAC 2 cut(s) 5, 384
SatI GCNGC 2 cut(s) 92, 260
Sau3AI GATC 1 cut(s) 116
Sau96I GGNCC 4 cut(s) 12, 141, 435, 436
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 2 cut(s) 35, 439
SetI ASST 3 cut(s) 210, 361, 495
SfaNI GCATC 2 cut(s) 515, 520
SfuI TTCGAA 1 cut(s) 465
SinI GGWCC 1 cut(s) 141
Sse9I AATT 6 cut(s) 53, 120, 160, 235, 319, 426
SsiI CCGC 4 cut(s) 59, 76, 94, 138
SspMI CTAG 3 cut(s) 245, 278, 540
StyD4I CCNGG 1 cut(s) 110
TaaI ACNGT 3 cut(s) 4, 355, 375
TaqI TCGA 1 cut(s) 465
TasI AATT 6 cut(s) 53, 120, 160, 235, 319, 426
TatI WGTACW 1 cut(s) 4
TfiI GAWTC 1 cut(s) 104
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 2 cut(s) 91, 259
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 6 cut(s) 120, 233, 244, 332, 410, 522
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 141
XapI RAATTY 3 cut(s) 235, 319, 426
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 308
XspI CTAG 3 cut(s) 245, 278, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.