Rmu_sc0000159.1_g000014

F-box WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000159.1
OG0003058
Physical Location & Seq
Forward (+)
28586 .. 30851
2266 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1584 bp
atgctcttgaccatgacgtcctttccgcaatcttctctttgcttgactgctgccaagacctggtcagttgttccttggtttgcaaaacctggaggaccgccatcgccaaatgagatggccggtgtgactcatagacttgcattgcttcaaagccgtcggattaatgttgatcactggaagggtcactctgctaatattgatcaatgccgaaacaaggatgggttgcttctcactggcagcagcaggaagaagttcatgcgtctgtggtctctgaaagaaagccgtaaatgtgtgtataaatttcaaggagaatatcgagttccagtagactatggccctttggttgattttgactttgatgagagcaagattgttggcttggtcggctcaaagaatagtcgaatatgcatatggaggcgcagtgggatgactaggatattaaacccctttaagtgcaaggtagccaatgttctcaaattcttagaccctgaggtgccagtgacatgcctagggttgagtgatgaaggaagtcggttgtccattcttgctggtggtggttcttctatcacagtttccactgtacttttacctgatcatgagtttgcagcaaaattgcatatagcaggcgagataaagactttgagttacaacccttgcggcaatgataggacagtgtttgctggatcaagcaatggatatgtgtacggttgggacctgaggacatgtagacttttgtgggaaacgagagtgagtcggggtgctgtgtgttcaatgcagcaccggaggaatgatgcatcaactttggttgtgggtggaatggatggtgtgcttcatctagtggatcagaacacagggaaagtggttactaaaattgtcttggacaccagctactgtgtactgccaagttctaaacctgcttcaagtacaagtggtggttgttatcaaaacatggtaattcagagattgagtggaaaaaggatttcacctgatgccgacattgtcagcattcctgcacctcccatcacctgcttggcagtggggatgaagaagctggttcatcaaaacgtggctctttcaatgtcggatctgattatcataagatttatgaatctgggtcccaaccatctctgtgataactcattgttctcacattattgctcctgcagtttggatgttgataacactaatgctctccccaagacagtgcattttctttgggtggctttttcactctggacaatctggaaggcaagaagtgaagttgtctatggacatccgtctcctaaacctgttggtaaagcgcacagaattggagcagctgttgcagagtttcttggcagttccttagtggctccatatacaggtgtcccccaagctgatatcggttctgaaattggagacttggtgtgccccaaaccctgctggaatttgataaagatgagttctaatgctttggctgatttgcttgccaggcaagccgaaaggaagatggttcttggaaattggataatgcaccctccatctgattttctcaaagttctgaggagtaatggattgcctgcacctccttctggtgtgatttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
F-box F-box-like WD40

Protein Analysis

527

Amino Acids

57.61

Weight (kDa)

9.29

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ACSF4 PF13570 196 - 292 5.4e-07 Beta-alanine-activating enzyme, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1034
AasI GACNNNNNNGTC 1 cut(s) 16
AatII GACGTC 1 cut(s) 20
Acc36I ACCTGC 2 cut(s) 922, 1034
AccB1I GGYRCC 1 cut(s) 493
AccB7I CCANNNNNTGG 1 cut(s) 60
AccI GTMKAC 2 cut(s) 327, 727
AciI CCGC 3 cut(s) 26, 98, 657
AclWI GGATC 3 cut(s) 691, 849, 1092
AcoI YGGCCR 1 cut(s) 117
AcsI RAATTY 3 cut(s) 299, 476, 1426
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 4 cut(s) 582, 704, 897, 925
AfiI CCNNNNNNNGG 4 cut(s) 60, 214, 1361, 1571
AflIII ACRYGT 1 cut(s) 722
AgsI TTSAA 5 cut(s) 149, 305, 771, 921, 1077
AjnI CCWGG 3 cut(s) 59, 88, 1469
AluBI AGCT 4 cut(s) 888, 1051, 1319, 1376
AluI AGCT 4 cut(s) 888, 1051, 1319, 1376
Alw26I GTCTC 3 cut(s) 273, 1284, 1392
AlwI GGATC 3 cut(s) 691, 849, 1092
AlwNI CAGNNNCTG 1 cut(s) 891
AoxI GGCC 2 cut(s) 117, 334
ApeKI GCWGC 6 cut(s) 50, 237, 240, 605, 775, 1316
ApoI RAATTY 3 cut(s) 299, 476, 1426
AseI ATTAAT 1 cut(s) 162
Asp700I GAANNNNTTC 1 cut(s) 251
AspA2I CCTAGG 1 cut(s) 508
AspLEI GCGC 2 cut(s) 420, 1303
AspS9I GGNCC 4 cut(s) 95, 335, 712, 1115
AsuHPI GGTGA 2 cut(s) 975, 1015
AvaII GGWCC 3 cut(s) 95, 712, 1115
AvrII CCTAGG 1 cut(s) 508
AxyI CCTNAGG 2 cut(s) 489, 716
BaeGI GKGCMC 1 cut(s) 1412
BanI GGYRCC 1 cut(s) 493
BbvI GCAGC 6 cut(s) 37, 249, 252, 617, 787, 1328
BccI CCATC 8 cut(s) 109, 109, 212, 815, 1028, 1131, 1483, 1528
BceAI ACGGC 2 cut(s) 138, 267
BciT130I CCWGG 3 cut(s) 61, 90, 1471
BclI TGATCA 3 cut(s) 169, 199, 592
BcoDI GTCTC 3 cut(s) 273, 1284, 1392
BfaI CTAG 3 cut(s) 432, 509, 836
BfmI CTRYAG 1 cut(s) 1162
BfuAI ACCTGC 2 cut(s) 922, 1034
BisI GCNGC 7 cut(s) 51, 238, 241, 606, 658, 776, 1317
BlnI CCTAGG 1 cut(s) 508
BlsI GCNGC 7 cut(s) 52, 239, 242, 607, 659, 777, 1318
Bme1390I CCNGG 3 cut(s) 61, 90, 1471
Bme18I GGWCC 3 cut(s) 95, 712, 1115
BmgT120I GGNCC 4 cut(s) 95, 335, 712, 1115
BmiI GGNNCC 5 cut(s) 495, 713, 1116, 1117, 1353
BmrFI CCNGG 3 cut(s) 61, 90, 1471
BmsI GCATC 3 cut(s) 781, 803, 979
BoxI GACNNNNGTC 1 cut(s) 1276
BpmI CTGGAG 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 74, 508
BsaWI WCCGGW 1 cut(s) 780
BsaXI ACNNNNNCTCC 6 cut(s) 406, 436, 1305, 1335, 1389, 1419
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 214, 1361, 1571
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 4 cut(s) 179, 238, 323, 497
Bse21I CCTNAGG 2 cut(s) 489, 716
Bse3DI GCAATG 3 cut(s) 140, 667, 697
BseBI CCWGG 3 cut(s) 61, 90, 1471
BseDI CCNNGG 2 cut(s) 74, 508
BseGI GGATG 6 cut(s) 223, 432, 826, 1047, 1177, 1273
BseLI CCNNNNNNNGG 4 cut(s) 60, 214, 1361, 1571
BseMI GCAATG 3 cut(s) 140, 667, 697
BseMII CTCAG 3 cut(s) 480, 707, 1532
BseNI ACTGG 4 cut(s) 179, 238, 323, 497
BseRI GAGGAG 1 cut(s) 1558
BseSI GKGCMC 1 cut(s) 1412
BseXI GCAGC 6 cut(s) 37, 249, 252, 617, 787, 1328
BsgI GTGCAG 2 cut(s) 996, 1545
BshFI GGCC 2 cut(s) 119, 336
BshNI GGYRCC 1 cut(s) 493
BsiSI CCGG 2 cut(s) 120, 781
BslFI GGGAC 3 cut(s) 725, 1101, 1352
BslI CCNNNNNNNGG 4 cut(s) 60, 214, 1361, 1571
BsmAI GTCTC 3 cut(s) 273, 1284, 1392
BsmBI CGTCTC 1 cut(s) 1284
BsmFI GGGAC 3 cut(s) 725, 1101, 1352
BsmI GAATGC 1 cut(s) 1005
BsnI GGCC 2 cut(s) 119, 336
Bso31I GGTCTC 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 1412
Bsp143I GATC 6 cut(s) 169, 199, 592, 683, 841, 1084
BspACI CCGC 3 cut(s) 26, 98, 657
BspANI GGCC 2 cut(s) 119, 336
BspCNI CTCAG 3 cut(s) 481, 708, 1533
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 5 cut(s) 495, 713, 1116, 1117, 1353
BspMAI CTGCAG 1 cut(s) 1166
BspMI ACCTGC 2 cut(s) 922, 1034
BspPI GGATC 3 cut(s) 691, 849, 1092
BspT107I GGYRCC 1 cut(s) 493
BspTNI GGTCTC 1 cut(s) 273
BsrDI GCAATG 3 cut(s) 140, 667, 697
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 4 cut(s) 179, 238, 323, 497
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 74, 508
BssMI GATC 6 cut(s) 169, 199, 592, 683, 841, 1084
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 2 cut(s) 74, 508
Bst2UI CCWGG 3 cut(s) 61, 90, 1471
Bst4CI ACNGT 6 cut(s) 571, 580, 673, 707, 893, 1204
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 1322
BstC8I GCNNGC 4 cut(s) 625, 1467, 1476, 1560
BstDEI CTNAG 5 cut(s) 481, 489, 716, 1345, 1541
BstF5I GGATG 6 cut(s) 223, 432, 826, 1047, 1177, 1273
BstHHI GCGC 2 cut(s) 420, 1303
BstKTI GATC 6 cut(s) 172, 202, 595, 686, 844, 1087
BstMAI GTCTC 3 cut(s) 273, 1284, 1392
BstMBI GATC 6 cut(s) 169, 199, 592, 683, 841, 1084
BstMWI GCNNNNNNNGC 4 cut(s) 384, 1322, 1471, 1475
BstNI CCWGG 3 cut(s) 61, 90, 1471
BstNSI RCATGY 2 cut(s) 507, 726
BstPAI GACNNNNGTC 1 cut(s) 1276
BstSCI CCNGG 3 cut(s) 59, 88, 1469
BstSFI CTRYAG 1 cut(s) 1162
BstSLI GKGCMC 1 cut(s) 1412
BstV1I GCAGC 6 cut(s) 37, 249, 252, 617, 787, 1328
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
Bsu36I CCTNAGG 2 cut(s) 489, 716
BsuRI GGCC 2 cut(s) 119, 336
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 6 cut(s) 223, 432, 826, 1047, 1177, 1273
BtsI GCAGTG 2 cut(s) 427, 1041
BtsIMutI CAGTG 8 cut(s) 172, 231, 427, 504, 576, 678, 1041, 1209
BveI ACCTGC 2 cut(s) 922, 1034
Cac8I GCNNGC 4 cut(s) 625, 1467, 1476, 1560
CaiI CAGNNNCTG 1 cut(s) 891
CciI TCATGA 1 cut(s) 595
CfoI GCGC 2 cut(s) 420, 1303
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 4 cut(s) 95, 335, 712, 1115
CseI GACGC 1 cut(s) 248
CsiI ACCWGGT 1 cut(s) 59
Csp6I GTAC 4 cut(s) 581, 703, 896, 924
CviAII CATG 6 cut(s) 13, 256, 504, 596, 723, 949
CviQI GTAC 4 cut(s) 581, 703, 896, 924
DdeI CTNAG 5 cut(s) 481, 489, 716, 1345, 1541
DpnI GATC 6 cut(s) 171, 201, 594, 685, 843, 1086
DpnII GATC 6 cut(s) 169, 199, 592, 683, 841, 1084
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 1 cut(s) 117
Eco130I CCWWGG 2 cut(s) 74, 508
Eco31I GGTCTC 1 cut(s) 273
Eco32I GATATC 1 cut(s) 1381
Eco47I GGWCC 3 cut(s) 95, 712, 1115
Eco81I CCTNAGG 2 cut(s) 489, 716
EcoO109I RGGNCCY 2 cut(s) 712, 1115
EcoRII CCWGG 3 cut(s) 59, 88, 1469
EcoRV GATATC 1 cut(s) 1381
EcoT14I CCWWGG 2 cut(s) 74, 508
EcoT22I ATGCAT 2 cut(s) 410, 796
ErhI CCWWGG 2 cut(s) 74, 508
Esp3I CGTCTC 1 cut(s) 1284
FaeI CATG 6 cut(s) 16, 259, 507, 599, 726, 952
FaqI GGGAC 3 cut(s) 725, 1101, 1352
FatI CATG 6 cut(s) 12, 255, 503, 595, 722, 948
FauNDI CATATG 1 cut(s) 410
FbaI TGATCA 3 cut(s) 169, 199, 592
FblI GTMKAC 2 cut(s) 327, 727
Fnu4HI GCNGC 7 cut(s) 51, 238, 241, 606, 658, 776, 1317
FokI GGATG 6 cut(s) 230, 439, 833, 1054, 1184, 1260
Fsp4HI GCNGC 7 cut(s) 51, 238, 241, 606, 658, 776, 1317
FspBI CTAG 3 cut(s) 432, 509, 836
GlaI GCGC 2 cut(s) 419, 1302
GluI GCNGC 7 cut(s) 51, 238, 241, 606, 658, 776, 1317
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 2 cut(s) 119, 336
HapII CCGG 2 cut(s) 120, 781
HgaI GACGC 1 cut(s) 248
HhaI GCGC 2 cut(s) 420, 1303
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 6 cut(s) 16, 259, 507, 599, 726, 952
Hin6I GCGC 2 cut(s) 418, 1301
HinP1I GCGC 2 cut(s) 418, 1301
HinfI GANTC 3 cut(s) 127, 751, 1108
HpaII CCGG 2 cut(s) 120, 781
HphI GGTGA 2 cut(s) 975, 1015
Hpy166II GTNNAC 4 cut(s) 328, 703, 728, 896
Hpy188I TCNGA 9 cut(s) 159, 273, 846, 960, 1084, 1089, 1390, 1525, 1542
Hpy188III TCNNGA 4 cut(s) 7, 596, 1234, 1243
Hpy8I GTNNAC 4 cut(s) 328, 703, 728, 896
Hpy99I CGWCG 1 cut(s) 159
HpyAV CCTTC 4 cut(s) 172, 518, 1240, 1578
HpyCH4III ACNGT 6 cut(s) 571, 580, 673, 707, 893, 1204
HpyCH4IV ACGT 2 cut(s) 17, 1065
HpyF10VI GCNNNNNNNGC 4 cut(s) 384, 1322, 1471, 1475
HpyF3I CTNAG 5 cut(s) 481, 489, 716, 1345, 1541
HpySE526I ACGT 2 cut(s) 17, 1065
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 6 cut(s) 16, 259, 507, 599, 726, 952
HspAI GCGC 2 cut(s) 418, 1301
KflI GGGWCCC 1 cut(s) 1115
Ksp22I TGATCA 3 cut(s) 169, 199, 592
Kzo9I GATC 6 cut(s) 169, 199, 592, 683, 841, 1084
LmnI GCTCC 3 cut(s) 1163, 1313, 1357
Lsp1109I GCAGC 6 cut(s) 37, 249, 252, 617, 787, 1328
LweI GCATC 3 cut(s) 781, 803, 979
MabI ACCWGGT 1 cut(s) 59
MaeI CTAG 3 cut(s) 432, 509, 836
MaeII ACGT 2 cut(s) 17, 1065
MaeIII GTNAC 5 cut(s) 124, 182, 499, 644, 862
MalI GATC 6 cut(s) 171, 201, 594, 685, 843, 1086
MboI GATC 6 cut(s) 169, 199, 592, 683, 841, 1084
MboII GAAGA 5 cut(s) 24, 259, 552, 1057, 1498
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 1 cut(s) 1412
MluCI AATT 9 cut(s) 299, 476, 611, 870, 954, 1308, 1392, 1426, 1501
MlyI GAGTC 2 cut(s) 121, 760
MmeI TCCRAC 2 cut(s) 137, 1062
MnlI CCTC 9 cut(s) 86, 408, 484, 711, 777, 1026, 1527, 1536, 1575
Mph1103I ATGCAT 2 cut(s) 410, 796
MroXI GAANNNNTTC 1 cut(s) 251
MseI TTAA 3 cut(s) 162, 440, 450
MslI CAYNNNNRTG 1 cut(s) 1128
MspA1I CMGCKG 1 cut(s) 1319
MspI CCGG 2 cut(s) 120, 781
MspR9I CCNGG 3 cut(s) 61, 90, 1471
Mva1269I GAATGC 1 cut(s) 1005
MvaI CCWGG 3 cut(s) 61, 90, 1471
MwoI GCNNNNNNNGC 4 cut(s) 384, 1322, 1471, 1475
NdeI CATATG 1 cut(s) 410
NdeII GATC 6 cut(s) 169, 199, 592, 683, 841, 1084
NlaIII CATG 6 cut(s) 16, 259, 507, 599, 726, 952
NlaIV GGNNCC 5 cut(s) 495, 713, 1116, 1117, 1353
NmuCI GTSAC 3 cut(s) 124, 182, 499
NsiI ATGCAT 2 cut(s) 410, 796
NspI RCATGY 2 cut(s) 507, 726
PagI TCATGA 1 cut(s) 595
PaqCI CACCTGC 1 cut(s) 1034
PciI ACATGT 1 cut(s) 722
PctI GAATGC 1 cut(s) 1005
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 1108
PflFI GACNNNGTC 2 cut(s) 61, 998
PflMI CCANNNNNTGG 1 cut(s) 60
PkrI GCNGC 7 cut(s) 52, 239, 242, 607, 659, 777, 1318
PleI GAGTC 2 cut(s) 121, 759
PpsI GAGTC 2 cut(s) 121, 759
PpuMI RGGWCCY 2 cut(s) 712, 1115
PscI ACATGT 1 cut(s) 722
PshAI GACNNNNGTC 1 cut(s) 1276
PshBI ATTAAT 1 cut(s) 162
Psp5II RGGWCCY 2 cut(s) 712, 1115
Psp6I CCWGG 3 cut(s) 59, 88, 1469
PspGI CCWGG 3 cut(s) 59, 88, 1469
PspN4I GGNNCC 5 cut(s) 495, 713, 1116, 1117, 1353
PspPI GGNCC 4 cut(s) 95, 335, 712, 1115
PspPPI RGGWCCY 2 cut(s) 712, 1115
PsrI GAACNNNNNNTAC 2 cut(s) 453, 485
PstI CTGCAG 1 cut(s) 1166
PstNI CAGNNNCTG 1 cut(s) 891
PsuI RGATCY 1 cut(s) 1084
PsyI GACNNNGTC 2 cut(s) 61, 998
PvuII CAGCTG 1 cut(s) 1319
RsaI GTAC 4 cut(s) 582, 704, 897, 925
RsaNI GTAC 4 cut(s) 581, 703, 896, 924
RseI CAYNNNNRTG 1 cut(s) 1128
SaqAI TTAA 3 cut(s) 162, 440, 450
SatI GCNGC 7 cut(s) 51, 238, 241, 606, 658, 776, 1317
Sau3AI GATC 6 cut(s) 169, 199, 592, 683, 841, 1084
Sau96I GGNCC 4 cut(s) 95, 335, 712, 1115
SchI GAGTC 2 cut(s) 121, 760
ScrFI CCNGG 3 cut(s) 61, 90, 1471
SduI GDGCHC 1 cut(s) 1412
SexAI ACCWGGT 1 cut(s) 59
SfaNI GCATC 3 cut(s) 781, 803, 979
SfcI CTRYAG 1 cut(s) 1162
SinI GGWCC 3 cut(s) 95, 712, 1115
SmiMI CAYNNNNRTG 1 cut(s) 1128
Sse9I AATT 9 cut(s) 299, 476, 611, 870, 954, 1308, 1392, 1426, 1501
SsiI CCGC 3 cut(s) 26, 98, 657
SspI AATATT 1 cut(s) 196
SspMI CTAG 3 cut(s) 432, 509, 836
StyD4I CCNGG 3 cut(s) 59, 88, 1469
StyI CCWWGG 2 cut(s) 74, 508
TaaI ACNGT 6 cut(s) 571, 580, 673, 707, 893, 1204
TaiI ACGT 2 cut(s) 20, 1068
TaqI TCGA 2 cut(s) 316, 400
TasI AATT 9 cut(s) 299, 476, 611, 870, 954, 1308, 1392, 1426, 1501
TatI WGTACW 3 cut(s) 580, 895, 923
TauI GCSGC 1 cut(s) 660
TfiI GAWTC 1 cut(s) 1108
Tru1I TTAA 3 cut(s) 162, 440, 450
Tru9I TTAA 3 cut(s) 162, 440, 450
TscAI CASTG 8 cut(s) 179, 238, 427, 504, 583, 678, 1041, 1209
TseFI GTSAC 3 cut(s) 124, 182, 499
TseI GCWGC 6 cut(s) 50, 237, 240, 605, 775, 1316
Tsp45I GTSAC 3 cut(s) 124, 182, 499
TspDTI ATGAA 6 cut(s) 244, 537, 821, 1046, 1058, 1121
TspGWI ACGGA 1 cut(s) 1266
TspRI CASTG 8 cut(s) 179, 238, 427, 504, 583, 678, 1041, 1209
Tth111I GACNNNGTC 2 cut(s) 61, 998
Van91I CCANNNNNTGG 1 cut(s) 60
VpaK11BI GGWCC 3 cut(s) 95, 712, 1115
VspI ATTAAT 1 cut(s) 162
XapI RAATTY 3 cut(s) 299, 476, 1426
XceI RCATGY 2 cut(s) 507, 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 1027
XmaJI CCTAGG 1 cut(s) 508
XmiI GTMKAC 2 cut(s) 327, 727
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 3 cut(s) 432, 509, 836
ZraI GACGTC 1 cut(s) 18
Zsp2I ATGCAT 2 cut(s) 410, 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.