Rmu_sc0000258.1_g000005

Protein of unknown function (DUF1279)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000258.1
OG0010849
Physical Location & Seq
Forward (+)
18278 .. 18917
640 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
atggccggtggtcgattccgcgagcttctgaagaagtacggcaaggtcgcgctgggcgtccactgctccgtctccgccgcctcaatcgccggactctacgtcgccatcaagaacaacgtcgatgtggagtccctgatcgacaagcttcccaatctaaaccctcttaaaagctctcatgaagagtctccaaaaccccaaaactaccaaggctccttcttcttccttccttccttttgcgtcaatggccggtggtcgattccgcgagcttctgaagaaaacaacgtcgatgtggagtccctgatcgacaagcttcccaatctaaaccctcttaaaagctctcatgaagagaaacccacctccgaaaatgggccgtcgacggctgtggttgaggagaggaagaggaatcggacggcggagctcgcagccaccagcggcggtgctctggcattggcttttctctgcaacaaggctctgtttccgattcgggttccgattacgctggcggtcacgcctcctttggccaagtttttggccaggaggaggcttatcaagaccggtgtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1279

Protein Analysis

187

Amino Acids

20.35

Weight (kDa)

9.57

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM210A-B_dom PF06916 5 - 171 1.2e-22 FAM210A/B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 374
AccII CGCG 3 cut(s) 21, 50, 262
AciI CCGC 8 cut(s) 19, 75, 78, 260, 413, 432, 435, 503
AcoI YGGCCR 4 cut(s) 3, 244, 519, 531
AcuI CTGAAG 2 cut(s) 50, 291
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 2 cut(s) 366, 540
AgeI ACCGGT 1 cut(s) 554
AhdI GACNNNNNGTC 1 cut(s) 98
AjnI CCWGG 1 cut(s) 533
AluBI AGCT 7 cut(s) 25, 145, 171, 266, 310, 336, 418
AluI AGCT 7 cut(s) 25, 145, 171, 266, 310, 336, 418
Alw21I GWGCWC 2 cut(s) 420, 442
Alw26I GTCTC 2 cut(s) 76, 189
AoxI GGCC 5 cut(s) 3, 244, 368, 519, 531
ApeKI GCWGC 1 cut(s) 422
AsiGI ACCGGT 1 cut(s) 554
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 368
BalI TGGCCA 2 cut(s) 521, 533
BanII GRGCYC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 420, 442
BbvI GCAGC 1 cut(s) 434
BccI CCATC 1 cut(s) 113
BceAI ACGGC 4 cut(s) 55, 355, 393, 426
BciT130I CCWGG 1 cut(s) 535
BcoDI GTCTC 2 cut(s) 76, 189
BisI GCNGC 3 cut(s) 78, 423, 433
BlsI GCNGC 3 cut(s) 79, 424, 434
Bme1390I CCNGG 1 cut(s) 535
BmeRI GACNNNNNGTC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 2 cut(s) 211, 489
BmrFI CCNGG 1 cut(s) 535
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 1 cut(s) 554
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 540
Bse118I RCCGGY 3 cut(s) 5, 246, 554
BseBI CCWGG 1 cut(s) 535
BseDI CCNNGG 1 cut(s) 205
BseLI CCNNNNNNNGG 2 cut(s) 366, 540
BseRI GAGGAG 2 cut(s) 404, 553
BseXI GCAGC 1 cut(s) 434
BseYI CCCAGC 1 cut(s) 52
Bsh1236I CGCG 3 cut(s) 21, 50, 262
BshFI GGCC 5 cut(s) 5, 246, 370, 521, 533
BshTI ACCGGT 1 cut(s) 554
BsiHKAI GWGCWC 2 cut(s) 420, 442
BsiSI CCGG 4 cut(s) 6, 90, 247, 555
BslFI GGGAC 2 cut(s) 115, 280
BslI CCNNNNNNNGG 2 cut(s) 366, 540
BsmAI GTCTC 2 cut(s) 76, 189
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 2 cut(s) 115, 280
BsnI GGCC 5 cut(s) 5, 246, 370, 521, 533
Bsp1286I GDGCHC 2 cut(s) 420, 442
Bsp143I GATC 2 cut(s) 135, 300
BspACI CCGC 8 cut(s) 19, 75, 78, 260, 413, 432, 435, 503
BspANI GGCC 5 cut(s) 5, 246, 370, 521, 533
BspFNI CGCG 3 cut(s) 21, 50, 262
BspHI TCATGA 2 cut(s) 175, 340
BspLI GGNNCC 2 cut(s) 211, 489
BsrFI RCCGGY 3 cut(s) 5, 246, 554
BssAI RCCGGY 3 cut(s) 5, 246, 554
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 2 cut(s) 135, 300
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 535
Bst6I CTCTTC 3 cut(s) 174, 339, 392
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 4 cut(s) 23, 264, 420, 501
BstFNI CGCG 3 cut(s) 21, 50, 262
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 2 cut(s) 138, 303
BstMAI GTCTC 2 cut(s) 76, 189
BstMBI GATC 2 cut(s) 135, 300
BstMWI GCNNNNNNNGC 4 cut(s) 63, 86, 243, 419
BstNI CCWGG 1 cut(s) 535
BstSCI CCNGG 1 cut(s) 533
BstUI CGCG 3 cut(s) 21, 50, 262
BstV1I GCAGC 1 cut(s) 434
BstXI CCANNNNNNTGG 1 cut(s) 529
BsuRI GGCC 5 cut(s) 5, 246, 370, 521, 533
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 1 cut(s) 61
Cac8I GCNNGC 4 cut(s) 23, 264, 420, 501
CciI TCATGA 2 cut(s) 175, 340
CfoI GCGC 1 cut(s) 52
Cfr10I RCCGGY 3 cut(s) 5, 246, 554
Cfr13I GGNCC 1 cut(s) 368
CseI GACGC 2 cut(s) 46, 226
Csp6I GTAC 1 cut(s) 37
CspAI ACCGGT 1 cut(s) 554
CviAII CATG 2 cut(s) 176, 341
CviQI GTAC 1 cut(s) 37
DpnI GATC 2 cut(s) 137, 302
DpnII GATC 2 cut(s) 135, 300
DriI GACNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 4 cut(s) 3, 244, 519, 531
Eam1104I CTCTTC 3 cut(s) 174, 339, 392
Eam1105I GACNNNNNGTC 1 cut(s) 98
EarI CTCTTC 3 cut(s) 174, 339, 392
EciI GGCGGA 2 cut(s) 64, 428
Ecl136II GAGCTC 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 420
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 2 cut(s) 50, 291
EcoICRI GAGCTC 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 205
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 2 cut(s) 179, 344
FaiI YATR 3 cut(s) 177, 342, 562
FaqI GGGAC 2 cut(s) 115, 280
FatI CATG 2 cut(s) 175, 340
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 3 cut(s) 78, 423, 433
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 3 cut(s) 78, 423, 433
GlaI GCGC 1 cut(s) 51
GluI GCNGC 3 cut(s) 78, 423, 433
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 5 cut(s) 5, 246, 370, 521, 533
HapII CCGG 4 cut(s) 6, 90, 247, 555
HgaI GACGC 2 cut(s) 46, 226
HhaI GCGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 2 cut(s) 179, 344
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HindIII AAGCTT 2 cut(s) 143, 308
HinfI GANTC 8 cut(s) 15, 93, 128, 182, 256, 293, 403, 481
HpaII CCGG 4 cut(s) 6, 90, 247, 555
Hpy166II GTNNAC 2 cut(s) 61, 375
Hpy188I TCNGA 6 cut(s) 30, 271, 361, 408, 480, 492
Hpy188III TCNNGA 4 cut(s) 109, 176, 341, 550
Hpy8I GTNNAC 2 cut(s) 61, 375
Hpy99I CGWCG 5 cut(s) 104, 122, 287, 376, 379
HpyAV CCTTC 3 cut(s) 223, 233, 237
HpyCH4IV ACGT 3 cut(s) 99, 117, 282
HpyCH4V TGCA 1 cut(s) 462
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 86, 243, 419
HpySE526I ACGT 3 cut(s) 99, 117, 282
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 2 cut(s) 179, 344
HspAI GCGC 1 cut(s) 50
Kzo9I GATC 2 cut(s) 135, 300
LmnI GCTCC 3 cut(s) 71, 215, 415
Lsp1109I GCAGC 1 cut(s) 434
MaeII ACGT 3 cut(s) 99, 117, 282
MaeIII GTNAC 1 cut(s) 505
MalI GATC 2 cut(s) 137, 302
MboI GATC 2 cut(s) 135, 300
MboII GAAGA 7 cut(s) 43, 191, 208, 211, 284, 356, 409
MhlI GDGCHC 2 cut(s) 420, 442
MlsI TGGCCA 2 cut(s) 521, 533
MluNI TGGCCA 2 cut(s) 521, 533
MlyI GAGTC 4 cut(s) 87, 137, 191, 302
Mox20I TGGCCA 2 cut(s) 521, 533
MscI TGGCCA 2 cut(s) 521, 533
MseI TTAA 2 cut(s) 165, 330
Msp20I TGGCCA 2 cut(s) 521, 533
MspA1I CMGCKG 1 cut(s) 432
MspI CCGG 4 cut(s) 6, 90, 247, 555
MspR9I CCNGG 1 cut(s) 535
MvaI CCWGG 1 cut(s) 535
MvnI CGCG 3 cut(s) 21, 50, 262
MwoI GCNNNNNNNGC 4 cut(s) 63, 86, 243, 419
NdeII GATC 2 cut(s) 135, 300
NlaIII CATG 2 cut(s) 179, 344
NlaIV GGNNCC 2 cut(s) 211, 489
NmuCI GTSAC 1 cut(s) 505
PagI TCATGA 2 cut(s) 175, 340
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 4 cut(s) 15, 256, 403, 481
PinAI ACCGGT 1 cut(s) 554
PkrI GCNGC 3 cut(s) 79, 424, 434
PleI GAGTC 4 cut(s) 87, 136, 190, 301
PpsI GAGTC 4 cut(s) 87, 136, 190, 301
Psp124BI GAGCTC 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 533
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 1 cut(s) 533
PspN4I GGNNCC 2 cut(s) 211, 489
PspPI GGNCC 1 cut(s) 368
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
SacI GAGCTC 1 cut(s) 420
SalI GTCGAC 1 cut(s) 373
SaqAI TTAA 2 cut(s) 165, 330
SatI GCNGC 3 cut(s) 78, 423, 433
Sau3AI GATC 2 cut(s) 135, 300
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 4 cut(s) 87, 137, 191, 302
ScrFI CCNGG 1 cut(s) 535
SduI GDGCHC 2 cut(s) 420, 442
SgrDI CGTCGACG 1 cut(s) 373
SsiI CCGC 8 cut(s) 19, 75, 78, 260, 413, 432, 435, 503
SstI GAGCTC 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 533
StyI CCWWGG 1 cut(s) 205
TaiI ACGT 3 cut(s) 102, 120, 285
TaqI TCGA 7 cut(s) 13, 120, 138, 254, 285, 303, 374
TauI GCSGC 2 cut(s) 80, 435
TfiI GAWTC 4 cut(s) 15, 256, 403, 481
Tru1I TTAA 2 cut(s) 165, 330
Tru9I TTAA 2 cut(s) 165, 330
TscAI CASTG 1 cut(s) 68
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 2 cut(s) 192, 357
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 1 cut(s) 68
XmiI GTMKAC 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.