Rmu_sc0000258.1_g000086

Protein POLLEN DEFECTIVE IN GUIDANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000258.1
OG0014815
Physical Location & Seq
Forward (+)
389097 .. 395402
6306 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
atgcagagcgtcgagccaaccaagcacgaccatggtctccgcttccctactctccatcatcgcaggcaattttgcttgccttcgttggaggaggcctcgcggcttccttgtccttcgagatgtttgactttttcagtcaaaagcaaccccttcctttctctccttccatctgatttcgatgttggctattcttgccgagatgtggatgccggtcttgttcggtataattgctccaagacttttgtgggatccaggggtagtggcgtggattctagttggcttctgggttggagaactccggtggctgcgctggatcgagacgaccttgatgcagacggcgccgacgtcgctggtttggctgacgacattagtgttgggcagcggtcgatggcagccattgggaagccagcattgagttggtgtgctgggtgcccagatcaaacctggctaaggagttgggctgctgctgggcccaaattagggatcatatgttgggcttcacttctccaggaatctggatctaagacccgggaggataagggcaaaagaaatctctttgctttcttttttcggttggctgtggtagcaatggctggactcgcagcagaaggtctgaagtatgacaaagtgattggtcaatctgctgatctcttcactcttcagaacgctggtgtggtattcgtctatgaaatgattatcgatatcgtaaagcacttgttcattgctaagttcaatgacataaagccctttgcatactctgagtttcttgaaggacctatgcaagtagacgttcaaacaggatcaagagctttacctactttcatcaactatactcatgatggtatagagctacatgtatag

Protein Analysis

286

Amino Acids

31.41

Weight (kDa)

6.16

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF747 PF05346 186 - 257 1.2e-10 Eukaryotic membrane protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccB1I GGYRCC 2 cut(s) 338, 429
AccI GTMKAC 1 cut(s) 786
AccII CGCG 1 cut(s) 100
AciI CCGC 3 cut(s) 40, 100, 382
AclWI GGATC 6 cut(s) 243, 256, 321, 491, 526, 808
AcuI CTGAAG 2 cut(s) 635, 644
AcyI GRCGYC 2 cut(s) 339, 345
AfiI CCNNNNNNNGG 4 cut(s) 202, 450, 479, 480
AflIII ACRYGT 1 cut(s) 853
AgsI TTSAA 3 cut(s) 733, 770, 794
AjnI CCWGG 3 cut(s) 251, 443, 507
AluBI AGCT 2 cut(s) 809, 850
AluI AGCT 2 cut(s) 809, 850
Alw26I GTCTC 2 cut(s) 41, 312
AlwI GGATC 6 cut(s) 243, 256, 321, 491, 526, 808
Ama87I CYCGRG 1 cut(s) 528
AoxI GGCC 2 cut(s) 93, 470
ApaI GGGCCC 1 cut(s) 474
ApeKI GCWGC 6 cut(s) 305, 379, 392, 461, 464, 602
AspLEI GCGC 2 cut(s) 310, 341
AspS9I GGNCC 3 cut(s) 470, 471, 773
AsuC2I CCSGG 2 cut(s) 529, 530
AvaI CYCGRG 1 cut(s) 528
AvaII GGWCC 1 cut(s) 773
BaeGI GKGCMC 2 cut(s) 434, 474
BamHI GGATCC 1 cut(s) 248
BanI GGYRCC 2 cut(s) 338, 429
BanII GRGCYC 1 cut(s) 474
BbvI GCAGC 6 cut(s) 292, 391, 404, 448, 451, 614
BccI CCATC 4 cut(s) 63, 175, 382, 833
BceAI ACGGC 1 cut(s) 352
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 3 cut(s) 253, 445, 509
BcnI CCSGG 2 cut(s) 529, 530
BcoDI GTCTC 2 cut(s) 41, 312
BfaI CTAG 1 cut(s) 273
BfoI RGCGCY 1 cut(s) 342
BisI GCNGC 7 cut(s) 101, 306, 380, 393, 462, 465, 603
BlsI GCNGC 7 cut(s) 102, 307, 381, 394, 463, 466, 604
Bme1390I CCNGG 5 cut(s) 253, 445, 509, 529, 530
Bme18I GGWCC 1 cut(s) 773
BmeT110I CYCGRG 1 cut(s) 528
BmgT120I GGNCC 3 cut(s) 470, 471, 773
BmiI GGNNCC 4 cut(s) 250, 340, 431, 472
BmrFI CCNGG 5 cut(s) 253, 445, 509, 529, 530
BmsI GCATC 2 cut(s) 196, 319
BoxI GACNNNNGTC 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 80, 112
BpmI CTGGAG 1 cut(s) 491
Bpu10I CCTNAGC 1 cut(s) 449
BpuMI CCSGG 2 cut(s) 529, 530
Bsa29I ATCGAT 1 cut(s) 699
BsaHI GRCGYC 2 cut(s) 339, 345
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 3 cut(s) 31, 252, 528
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 450, 479, 480
Bse118I RCCGGY 1 cut(s) 209
Bse3DI GCAATG 2 cut(s) 594, 720
BseBI CCWGG 3 cut(s) 253, 445, 509
BseCI ATCGAT 1 cut(s) 699
BseDI CCNNGG 3 cut(s) 31, 252, 528
BseGI GGATG 1 cut(s) 211
BseLI CCNNNNNNNGG 4 cut(s) 202, 450, 479, 480
BseMI GCAATG 2 cut(s) 594, 720
BseMII CTCAG 1 cut(s) 750
BseRI GAGGAG 1 cut(s) 104
BseSI GKGCMC 2 cut(s) 434, 474
BseXI GCAGC 6 cut(s) 292, 391, 404, 448, 451, 614
BseYI CCCAGC 2 cut(s) 425, 467
Bsh1236I CGCG 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 386
BshFI GGCC 2 cut(s) 95, 472
BshNI GGYRCC 2 cut(s) 338, 429
BshVI ATCGAT 1 cut(s) 699
BsiEI CGRYCG 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 528
BsiSI CCGG 3 cut(s) 210, 299, 529
BslI CCNNNNNNNGG 4 cut(s) 202, 450, 479, 480
BsmAI GTCTC 2 cut(s) 41, 312
BsmBI CGTCTC 1 cut(s) 312
BsnI GGCC 2 cut(s) 95, 472
Bso31I GGTCTC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 528
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 2 cut(s) 434, 474
Bsp143I GATC 7 cut(s) 248, 313, 436, 483, 518, 646, 800
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 3 cut(s) 40, 100, 382
BspANI GGCC 2 cut(s) 95, 472
BspCNI CTCAG 1 cut(s) 751
BspDI ATCGAT 1 cut(s) 699
BspFNI CGCG 1 cut(s) 100
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 4 cut(s) 250, 340, 431, 472
BspPI GGATC 6 cut(s) 243, 256, 321, 491, 526, 808
BspT107I GGYRCC 2 cut(s) 338, 429
BspTNI GGTCTC 1 cut(s) 41
BsrDI GCAATG 2 cut(s) 594, 720
BsrFI RCCGGY 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 3 cut(s) 31, 252, 528
BssMI GATC 7 cut(s) 248, 313, 436, 483, 518, 646, 800
BssNI GRCGYC 2 cut(s) 339, 345
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 3 cut(s) 253, 445, 509
Bst6I CTCTTC 2 cut(s) 656, 663
BstACI GRCGYC 2 cut(s) 339, 345
BstC8I GCNNGC 3 cut(s) 65, 77, 408
BstDEI CTNAG 4 cut(s) 449, 522, 726, 759
BstDSI CCRYGG 1 cut(s) 31
BstENI CCTNNNNNAGG 1 cut(s) 448
BstF5I GGATG 1 cut(s) 211
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 2 cut(s) 310, 341
BstKTI GATC 7 cut(s) 251, 316, 439, 486, 521, 649, 803
BstMAI GTCTC 2 cut(s) 41, 312
BstMBI GATC 7 cut(s) 248, 313, 436, 483, 518, 646, 800
BstMCI CGRYCG 1 cut(s) 386
BstMWI GCNNNNNNNGC 7 cut(s) 22, 192, 338, 347, 356, 584, 599
BstNI CCWGG 3 cut(s) 253, 445, 509
BstNSI RCATGY 1 cut(s) 857
BstPAI GACNNNNGTC 1 cut(s) 33
BstSCI CCNGG 5 cut(s) 251, 443, 507, 527, 528
BstSLI GKGCMC 2 cut(s) 434, 474
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 6 cut(s) 292, 391, 404, 448, 451, 614
BstX2I RGATCY 2 cut(s) 248, 518
BstXI CCANNNNNNTGG 1 cut(s) 515
BstYI RGATCY 2 cut(s) 248, 518
Bsu15I ATCGAT 1 cut(s) 699
BsuRI GGCC 2 cut(s) 95, 472
BsuTUI ATCGAT 1 cut(s) 699
BtgI CCRYGG 1 cut(s) 31
BtgZI GCGATG 1 cut(s) 44
BtsCI GGATG 1 cut(s) 211
Cac8I GCNNGC 3 cut(s) 65, 77, 408
CciI TCATGA 1 cut(s) 835
CfoI GCGC 2 cut(s) 310, 341
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 3 cut(s) 470, 471, 773
Cfr9I CCCGGG 1 cut(s) 528
ClaI ATCGAT 1 cut(s) 699
CviAII CATG 3 cut(s) 32, 836, 854
DdeI CTNAG 4 cut(s) 449, 522, 726, 759
DinI GGCGCC 1 cut(s) 340
DpnI GATC 7 cut(s) 250, 315, 438, 485, 520, 648, 802
DpnII GATC 7 cut(s) 248, 313, 436, 483, 518, 646, 800
Eam1104I CTCTTC 2 cut(s) 656, 663
EarI CTCTTC 2 cut(s) 656, 663
Eco130I CCWWGG 1 cut(s) 31
Eco147I AGGCCT 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 474
Eco31I GGTCTC 1 cut(s) 41
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 1 cut(s) 773
Eco57I CTGAAG 2 cut(s) 635, 644
Eco88I CYCGRG 1 cut(s) 528
EcoNI CCTNNNNNAGG 1 cut(s) 448
EcoO109I RGGNCCY 1 cut(s) 773
EcoRII CCWGG 3 cut(s) 251, 443, 507
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 474
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 3 cut(s) 35, 839, 857
FatI CATG 3 cut(s) 31, 835, 853
FauNDI CATATG 1 cut(s) 488
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 7 cut(s) 101, 306, 380, 393, 462, 465, 603
FokI GGATG 1 cut(s) 218
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 7 cut(s) 101, 306, 380, 393, 462, 465, 603
FspBI CTAG 1 cut(s) 273
GlaI GCGC 2 cut(s) 309, 340
GluI GCNGC 7 cut(s) 101, 306, 380, 393, 462, 465, 603
GsaI CCCAGC 2 cut(s) 429, 471
GsuI CTGGAG 1 cut(s) 491
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 2 cut(s) 95, 472
HapII CCGG 3 cut(s) 210, 299, 529
HhaI GCGC 2 cut(s) 310, 341
Hin1I GRCGYC 2 cut(s) 339, 345
Hin1II CATG 3 cut(s) 35, 839, 857
Hin6I GCGC 2 cut(s) 308, 339
HinP1I GCGC 2 cut(s) 308, 339
HinfI GANTC 3 cut(s) 269, 512, 597
HpaII CCGG 3 cut(s) 210, 299, 529
Hpy166II GTNNAC 1 cut(s) 787
Hpy188I TCNGA 4 cut(s) 172, 615, 663, 760
Hpy188III TCNNGA 6 cut(s) 117, 317, 516, 767, 804, 836
Hpy8I GTNNAC 1 cut(s) 787
Hpy99I CGWCG 3 cut(s) 14, 347, 350
HpyAV CCTTC 6 cut(s) 90, 123, 160, 173, 602, 764
HpyCH4IV ACGT 2 cut(s) 345, 789
HpyCH4V TGCA 4 cut(s) 4, 332, 752, 781
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 192, 338, 347, 356, 584, 599
HpyF3I CTNAG 4 cut(s) 449, 522, 726, 759
HpySE526I ACGT 2 cut(s) 345, 789
Hsp92I GRCGYC 2 cut(s) 339, 345
Hsp92II CATG 3 cut(s) 35, 839, 857
HspAI GCGC 2 cut(s) 308, 339
KasI GGCGCC 1 cut(s) 338
Kzo9I GATC 7 cut(s) 248, 313, 436, 483, 518, 646, 800
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 6 cut(s) 292, 391, 404, 448, 451, 614
LweI GCATC 2 cut(s) 196, 319
MaeI CTAG 1 cut(s) 273
MaeII ACGT 2 cut(s) 345, 789
MalI GATC 7 cut(s) 250, 315, 438, 485, 520, 648, 802
MboI GATC 7 cut(s) 248, 313, 436, 483, 518, 646, 800
MboII GAAGA 2 cut(s) 643, 650
MflI RGATCY 2 cut(s) 248, 518
MhlI GDGCHC 2 cut(s) 434, 474
MluCI AATT 3 cut(s) 68, 226, 476
Mly113I GGCGCC 1 cut(s) 339
MlyI GAGTC 1 cut(s) 591
MmeI TCCRAC 2 cut(s) 66, 269
MnlI CCTC 4 cut(s) 82, 85, 106, 526
MslI CAYNNNNRTG 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 382
MspI CCGG 3 cut(s) 210, 299, 529
MspR9I CCNGG 5 cut(s) 253, 445, 509, 529, 530
MvaI CCWGG 3 cut(s) 253, 445, 509
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 7 cut(s) 22, 192, 338, 347, 356, 584, 599
NarI GGCGCC 1 cut(s) 339
NciI CCSGG 2 cut(s) 529, 530
NcoI CCATGG 1 cut(s) 31
NdeI CATATG 1 cut(s) 488
NdeII GATC 7 cut(s) 248, 313, 436, 483, 518, 646, 800
NlaIII CATG 3 cut(s) 35, 839, 857
NlaIV GGNNCC 4 cut(s) 250, 340, 431, 472
NmeAIII GCCGAG 1 cut(s) 221
NspI RCATGY 1 cut(s) 857
PagI TCATGA 1 cut(s) 835
PceI AGGCCT 1 cut(s) 95
PciI ACATGT 1 cut(s) 853
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 2 cut(s) 269, 512
PfoI TCCNGGA 1 cut(s) 507
PkrI GCNGC 7 cut(s) 102, 307, 381, 394, 463, 466, 604
PleI GAGTC 1 cut(s) 591
PluTI GGCGCC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 591
PpuMI RGGWCCY 1 cut(s) 773
PscI ACATGT 1 cut(s) 853
PshAI GACNNNNGTC 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 773
Psp6I CCWGG 3 cut(s) 251, 443, 507
PspFI CCCAGC 2 cut(s) 425, 467
PspGI CCWGG 3 cut(s) 251, 443, 507
PspN4I GGNNCC 4 cut(s) 250, 340, 431, 472
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 3 cut(s) 470, 471, 773
PspPPI RGGWCCY 1 cut(s) 773
PsuI RGATCY 2 cut(s) 248, 518
RseI CAYNNNNRTG 1 cut(s) 30
SatI GCNGC 7 cut(s) 101, 306, 380, 393, 462, 465, 603
Sau3AI GATC 7 cut(s) 248, 313, 436, 483, 518, 646, 800
Sau96I GGNCC 3 cut(s) 470, 471, 773
SchI GAGTC 1 cut(s) 591
ScrFI CCNGG 5 cut(s) 253, 445, 509, 529, 530
SduI GDGCHC 2 cut(s) 434, 474
SetI ASST 9 cut(s) 327, 348, 446, 613, 778, 792, 811, 817, 852
SfaNI GCATC 2 cut(s) 196, 319
SfoI GGCGCC 1 cut(s) 340
SinI GGWCC 1 cut(s) 773
SmaI CCCGGG 1 cut(s) 530
SmiMI CAYNNNNRTG 1 cut(s) 30
Sse9I AATT 3 cut(s) 68, 226, 476
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 3 cut(s) 40, 100, 382
SspDI GGCGCC 1 cut(s) 338
SspMI CTAG 1 cut(s) 273
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 5 cut(s) 251, 443, 507, 527, 528
StyI CCWWGG 1 cut(s) 31
TaiI ACGT 2 cut(s) 348, 792
TaqI TCGA 6 cut(s) 12, 116, 177, 316, 386, 699
TasI AATT 3 cut(s) 68, 226, 476
TauI GCSGC 1 cut(s) 103
TfiI GAWTC 2 cut(s) 269, 512
TseI GCWGC 6 cut(s) 305, 379, 392, 461, 464, 602
TspDTI ATGAA 3 cut(s) 702, 709, 811
TspMI CCCGGG 1 cut(s) 528
VpaK11BI GGWCC 1 cut(s) 773
XagI CCTNNNNNAGG 1 cut(s) 448
XceI RCATGY 1 cut(s) 857
XcmI CCANNNNNNNNNTGG 3 cut(s) 241, 414, 441
XmaI CCCGGG 1 cut(s) 528
XmiI GTMKAC 1 cut(s) 786
XspI CTAG 1 cut(s) 273
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.