Rh5DG211000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014815
Physical Location & Seq
Forward (+)
23638369 .. 23638782
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG211000.1

Sequence Viewer

Length: 414 bp
ATGCAGAGCGTCGAATCTTACGAGCACGGCCATGGCCGACGCTCCTCTACCCTCCATCACCGCACGCGTATTTGCTTGCCTTTGTTGGTGGCGCATAGGTGGAGGCCTCATGGCTTCCTTATCTCTGGAGGTGATCGTCGTTTTGCGGCGAAAGCAATCCTGTACCTCTCATTGACAATTTCACTTTGCTTCCACCTGATTTCGGTGATACTTGCCGAAATGTGGATGCAGATCATGTTGATGATTGCTCCAAGAGGCTTGTGGGATCCAGGTCCAAGTAATCTTGCGGTGACTTTCACTGGTAGTACTGGGATTGCCTTGTTAATACTGGTATCAGTGGTGATGTCGGGCGGGGGATCTGGAGTTTCACTATTGCTGGCGTCTTTGTCGGAATTGCAGATGTCTCCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.95

Weight (kDa)

9.38

Isoelectric Point (pI)

55.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 67
AciI CCGC 4 cut(s) 61, 146, 287, 351
AclWI GGATC 3 cut(s) 260, 273, 364
AcoI YGGCCR 2 cut(s) 28, 34
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 2 cut(s) 164, 307
AfiI CCNNNNNNNGG 2 cut(s) 202, 222
AflIII ACRYGT 1 cut(s) 65
AjnI CCWGG 1 cut(s) 268
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 3 cut(s) 260, 273, 364
AoxI GGCC 3 cut(s) 28, 34, 104
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 5 cut(s) 50, 143, 217, 301, 352
AvaII GGWCC 1 cut(s) 272
BamHI GGATCC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 27
BccI CCATC 1 cut(s) 63
BceAI ACGGC 1 cut(s) 43
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 408
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
BmcAI AGTACT 1 cut(s) 307
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 270
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 216
BmuI ACTGGG 1 cut(s) 318
BpmI CTGGAG 3 cut(s) 147, 381, 390
BsaBI GATNNNNATC 1 cut(s) 230
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 222
Bse1I ACTGG 3 cut(s) 304, 313, 333
Bse8I GATNNNNATC 1 cut(s) 230
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 1 cut(s) 231
BseJI GATNNNNATC 1 cut(s) 230
BseLI CCNNNNNNNGG 2 cut(s) 202, 222
BseNI ACTGG 3 cut(s) 304, 313, 333
BseRI GAGGAG 1 cut(s) 34
Bsh1236I CGCG 1 cut(s) 67
BshFI GGCC 3 cut(s) 30, 36, 106
BsiHKAI GWGCWC 1 cut(s) 27
BslI CCNNNNNNNGG 2 cut(s) 202, 222
BsmAI GTCTC 1 cut(s) 408
BsnI GGCC 3 cut(s) 30, 36, 106
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 4 cut(s) 133, 231, 265, 356
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 4 cut(s) 61, 146, 287, 351
BspANI GGCC 3 cut(s) 30, 36, 106
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 3 cut(s) 260, 273, 364
BsrI ACTGG 3 cut(s) 304, 313, 333
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 4 cut(s) 133, 231, 265, 356
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 380
BstC8I GCNNGC 3 cut(s) 65, 77, 378
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 1 cut(s) 231
BstFNI CGCG 1 cut(s) 67
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 4 cut(s) 136, 234, 268, 359
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 4 cut(s) 133, 231, 265, 356
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 268
BstUI CGCG 1 cut(s) 67
BstX2I RGATCY 2 cut(s) 265, 356
BstYI RGATCY 2 cut(s) 265, 356
BsuRI GGCC 3 cut(s) 30, 36, 106
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 297, 342
Cac8I GCNNGC 3 cut(s) 65, 77, 378
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 272
CseI GACGC 2 cut(s) 48, 369
Csp6I GTAC 2 cut(s) 163, 306
CviAII CATG 3 cut(s) 32, 110, 235
CviJI RGCY 5 cut(s) 30, 36, 106, 114, 258
CviKI_1 RGCY 5 cut(s) 30, 36, 106, 114, 258
CviQI GTAC 2 cut(s) 163, 306
DpnI GATC 4 cut(s) 135, 233, 267, 358
DpnII GATC 4 cut(s) 133, 231, 265, 356
EaeI YGGCCR 2 cut(s) 28, 34
Eco130I CCWWGG 1 cut(s) 31
Eco147I AGGCCT 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 3 cut(s) 35, 113, 238
FaiI YATR 4 cut(s) 33, 96, 111, 236
FatI CATG 3 cut(s) 31, 109, 234
FauI CCCGC 1 cut(s) 344
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 238
Fsp4HI GCNGC 1 cut(s) 147
GlaI GCGC 1 cut(s) 93
GluI GCNGC 1 cut(s) 147
GsuI CTGGAG 3 cut(s) 147, 381, 390
HaeIII GGCC 3 cut(s) 30, 36, 106
HgaI GACGC 2 cut(s) 48, 369
HhaI GCGC 1 cut(s) 94
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 3 cut(s) 35, 113, 238
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HinfI GANTC 1 cut(s) 14
HphI GGTGA 5 cut(s) 50, 143, 217, 301, 352
Hpy188I TCNGA 1 cut(s) 391
Hpy188III TCNNGA 3 cut(s) 126, 360, 407
Hpy99I CGWCG 3 cut(s) 14, 42, 141
HpyCH4V TGCA 3 cut(s) 4, 229, 397
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 3 cut(s) 35, 113, 238
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 4 cut(s) 133, 231, 265, 356
LmnI GCTCC 2 cut(s) 47, 253
LweI GCATC 1 cut(s) 216
MaeIII GTNAC 1 cut(s) 289
MalI GATC 4 cut(s) 135, 233, 267, 358
MboI GATC 4 cut(s) 133, 231, 265, 356
MflI RGATCY 2 cut(s) 265, 356
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 2 cut(s) 177, 392
MluI ACGCGT 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 7 cut(s) 55, 62, 96, 117, 122, 176, 248
MseI TTAA 1 cut(s) 323
MslI CAYNNNNRTG 2 cut(s) 30, 239
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 1 cut(s) 152
NcoI CCATGG 1 cut(s) 31
NdeII GATC 4 cut(s) 133, 231, 265, 356
NlaIII CATG 3 cut(s) 35, 113, 238
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 289
PceI AGGCCT 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 272
PsuI RGATCY 2 cut(s) 265, 356
RsaI GTAC 2 cut(s) 164, 307
RsaNI GTAC 2 cut(s) 163, 306
RseI CAYNNNNRTG 2 cut(s) 30, 239
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 4 cut(s) 133, 231, 265, 356
Sau96I GGNCC 1 cut(s) 272
ScaI AGTACT 1 cut(s) 307
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 27
SetI ASST 5 cut(s) 101, 133, 168, 198, 274
SfaNI GCATC 1 cut(s) 216
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 2 cut(s) 30, 239
Sse9I AATT 2 cut(s) 177, 392
SseBI AGGCCT 1 cut(s) 106
SsiI CCGC 4 cut(s) 61, 146, 287, 351
StuI AGGCCT 1 cut(s) 106
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 1 cut(s) 31
TaqI TCGA 1 cut(s) 12
TasI AATT 2 cut(s) 177, 392
TatI WGTACW 1 cut(s) 305
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 2 cut(s) 304, 342
TseFI GTSAC 1 cut(s) 289
Tsp45I GTSAC 1 cut(s) 289
TspRI CASTG 2 cut(s) 304, 342
VpaK11BI GGWCC 1 cut(s) 272
XcmI CCANNNNNNNNNTGG 1 cut(s) 258
ZrmI AGTACT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.