Rh4DG324600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014815
Physical Location & Seq
Forward (+)
54936721 .. 54937173
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG324600.1

Sequence Viewer

Length: 453 bp
ATGCAGAGCGTCGAGTCTTACGAGCACGGCCATGGCCGCCACTCCTCTACCCTCCATCATCGCACGCGTATTTGCTTGCCTTTGTTGGTGGCGCATAGGTGGAGGCCTCATGGCTTCCTTATCTCTGGAGGTGATCGTCGTTTTGCGGCGAAAGCAATCCTGTACCTCTCCTTGACAATTTCACTTTGCTTCCACCTGATTTCGGTGATACTTGCCGAAATGTGGATGCAGATCATGTTGGTTATTGCTCCAAGAGGCTTGTGGGATCCAGGTCGAAGTAATCTTGCGGTGACTTTCACTGGTAGTACTGGGATTGCCTTGTTAATACTGGAATTAGTAGTGATGTCGGGCGGGGGATCTGGAGTTTCGCTATTGTTGGCGTCTTTGCCGGAATTGCAGATGTCTCCAGAACAGGAGGCGGTCAGACGTGGGATCTGGATGTGCTGGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.61

Weight (kDa)

9.1

Isoelectric Point (pI)

56.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 67
AciI CCGC 5 cut(s) 37, 146, 287, 351, 419
AclWI GGATC 4 cut(s) 260, 273, 364, 440
AcoI YGGCCR 2 cut(s) 28, 34
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 2 cut(s) 164, 307
AfiI CCNNNNNNNGG 2 cut(s) 202, 222
AflIII ACRYGT 1 cut(s) 65
AjiI CACGTC 1 cut(s) 428
AjnI CCWGG 1 cut(s) 268
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 4 cut(s) 260, 273, 364, 440
AoxI GGCC 3 cut(s) 28, 34, 104
AspLEI GCGC 1 cut(s) 94
AsuHPI GGTGA 3 cut(s) 143, 217, 301
BamHI GGATCC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 27
BccI CCATC 1 cut(s) 63
BceAI ACGGC 1 cut(s) 43
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 408
BisI GCNGC 2 cut(s) 37, 147
BlsI GCNGC 2 cut(s) 38, 148
BmcAI AGTACT 1 cut(s) 307
Bme1390I CCNGG 1 cut(s) 270
BmgBI CACGTC 1 cut(s) 428
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 270
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 216
BmuI ACTGGG 1 cut(s) 318
BpmI CTGGAG 3 cut(s) 147, 381, 390
BsaBI GATNNNNATC 1 cut(s) 230
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 222
Bse1I ACTGG 3 cut(s) 304, 313, 333
Bse8I GATNNNNATC 1 cut(s) 230
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 2 cut(s) 231, 444
BseJI GATNNNNATC 1 cut(s) 230
BseLI CCNNNNNNNGG 2 cut(s) 202, 222
BseNI ACTGG 3 cut(s) 304, 313, 333
BseRI GAGGAG 1 cut(s) 34
BseYI CCCAGC 1 cut(s) 444
Bsh1236I CGCG 1 cut(s) 67
BshFI GGCC 3 cut(s) 30, 36, 106
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 1 cut(s) 389
BslI CCNNNNNNNGG 2 cut(s) 202, 222
BsmAI GTCTC 1 cut(s) 408
BsnI GGCC 3 cut(s) 30, 36, 106
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 5 cut(s) 133, 231, 265, 356, 432
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 5 cut(s) 37, 146, 287, 351, 419
BspANI GGCC 3 cut(s) 30, 36, 106
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 4 cut(s) 260, 273, 364, 440
BsrI ACTGG 3 cut(s) 304, 313, 333
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 5 cut(s) 133, 231, 265, 356, 432
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 380
BstC8I GCNNGC 2 cut(s) 65, 77
BstDEI CTNAG 1 cut(s) 450
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 2 cut(s) 231, 444
BstFNI CGCG 1 cut(s) 67
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 5 cut(s) 136, 234, 268, 359, 435
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 5 cut(s) 133, 231, 265, 356, 432
BstMWI GCNNNNNNNGC 3 cut(s) 36, 152, 394
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 268
BstUI CGCG 1 cut(s) 67
BstX2I RGATCY 3 cut(s) 265, 356, 432
BstYI RGATCY 3 cut(s) 265, 356, 432
BsuRI GGCC 3 cut(s) 30, 36, 106
BtgI CCRYGG 1 cut(s) 31
BtgZI GCGATG 1 cut(s) 44
BtrI CACGTC 1 cut(s) 428
BtsCI GGATG 2 cut(s) 231, 444
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 2 cut(s) 65, 77
CfoI GCGC 1 cut(s) 94
CseI GACGC 1 cut(s) 369
Csp6I GTAC 2 cut(s) 163, 306
CviAII CATG 3 cut(s) 32, 110, 235
CviJI RGCY 6 cut(s) 30, 36, 106, 114, 258, 449
CviKI_1 RGCY 6 cut(s) 30, 36, 106, 114, 258, 449
CviQI GTAC 2 cut(s) 163, 306
DdeI CTNAG 1 cut(s) 450
DpnI GATC 5 cut(s) 135, 233, 267, 358, 434
DpnII GATC 5 cut(s) 133, 231, 265, 356, 432
EaeI YGGCCR 2 cut(s) 28, 34
Eco130I CCWWGG 1 cut(s) 31
Eco147I AGGCCT 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 3 cut(s) 35, 113, 238
FaiI YATR 4 cut(s) 33, 96, 111, 236
FatI CATG 3 cut(s) 31, 109, 234
FauI CCCGC 1 cut(s) 344
Fnu4HI GCNGC 2 cut(s) 37, 147
FokI GGATG 1 cut(s) 238
Fsp4HI GCNGC 2 cut(s) 37, 147
GlaI GCGC 1 cut(s) 93
GluI GCNGC 2 cut(s) 37, 147
GsaI CCCAGC 1 cut(s) 448
GsuI CTGGAG 3 cut(s) 147, 381, 390
HaeIII GGCC 3 cut(s) 30, 36, 106
HapII CCGG 1 cut(s) 389
HgaI GACGC 1 cut(s) 369
HhaI GCGC 1 cut(s) 94
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 3 cut(s) 35, 113, 238
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HinfI GANTC 1 cut(s) 14
HpaII CCGG 1 cut(s) 389
HphI GGTGA 3 cut(s) 143, 217, 301
Hpy188I TCNGA 1 cut(s) 425
Hpy188III TCNNGA 4 cut(s) 126, 360, 407, 436
Hpy99I CGWCG 2 cut(s) 14, 141
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 3 cut(s) 4, 229, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 152, 394
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 1 cut(s) 427
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 3 cut(s) 35, 113, 238
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 5 cut(s) 133, 231, 265, 356, 432
LmnI GCTCC 1 cut(s) 253
LweI GCATC 1 cut(s) 216
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 1 cut(s) 289
MalI GATC 5 cut(s) 135, 233, 267, 358, 434
MboI GATC 5 cut(s) 133, 231, 265, 356, 432
MflI RGATCY 3 cut(s) 265, 356, 432
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 3 cut(s) 177, 332, 392
MluI ACGCGT 1 cut(s) 65
MlyI GAGTC 1 cut(s) 23
MnlI CCTC 8 cut(s) 55, 62, 96, 117, 122, 176, 248, 409
MseI TTAA 1 cut(s) 323
MslI CAYNNNNRTG 1 cut(s) 30
MspI CCGG 1 cut(s) 389
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 3 cut(s) 36, 152, 394
NcoI CCATGG 1 cut(s) 31
NdeII GATC 5 cut(s) 133, 231, 265, 356, 432
NlaIII CATG 3 cut(s) 35, 113, 238
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 289
PceI AGGCCT 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PkrI GCNGC 2 cut(s) 38, 148
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Psp6I CCWGG 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 1 cut(s) 267
PsuI RGATCY 3 cut(s) 265, 356, 432
RsaI GTAC 2 cut(s) 164, 307
RsaNI GTAC 2 cut(s) 163, 306
RseI CAYNNNNRTG 1 cut(s) 30
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 2 cut(s) 37, 147
Sau3AI GATC 5 cut(s) 133, 231, 265, 356, 432
ScaI AGTACT 1 cut(s) 307
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 27
SetI ASST 6 cut(s) 101, 133, 168, 198, 274, 430
SfaNI GCATC 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 30
Sse9I AATT 3 cut(s) 177, 332, 392
SseBI AGGCCT 1 cut(s) 106
SsiI CCGC 5 cut(s) 37, 146, 287, 351, 419
StuI AGGCCT 1 cut(s) 106
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 1 cut(s) 31
TaiI ACGT 1 cut(s) 430
TaqI TCGA 2 cut(s) 12, 274
TasI AATT 3 cut(s) 177, 332, 392
TatI WGTACW 1 cut(s) 305
TauI GCSGC 2 cut(s) 39, 149
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 289
Tsp45I GTSAC 1 cut(s) 289
TspRI CASTG 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 258
ZrmI AGTACT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.