Rh4AG109300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014815
Physical Location & Seq
Reverse (-)
23694254 .. 23695578
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG109300.1

Sequence Viewer

Length: 465 bp
ATGCGTATGTCAAACTCCACGAGCATGGCCACGGCCGCCGCTCCTCTACGCTCCATCATCGCATGCAATTTTGCTTGTCCTTGTTGGTGGCTCATAAGAGGAAGCCTCTCGACTTTCTTGTCTTTTAGGGTGTTCAATATTTTGTGGCGAAAGCAATCTGTAACCTTTCTCAGCAAATTCATTTTGTTTCCATCTGATTTGGGTGTTGCTTATTCTTGCCGAGATGTGGATGCGGGTCACGTTAGGTATTATTGCCCAAAGAGATCTGTAGAATTCAAGTCCAAGCAAGGTTGTAGTTGTGGAATCTTTGGCTTCTGGGTTGGAGAACTCGAGGGCGCTTGGCTTCTGGGCATGGTTATGGTAATGCTTGGTGATGCGTCTCCCTTTGGATTTGCAGGGAGGGCGATGGTCGTAATGGTGGCGATGTCGACAGCCTTGGGGTACCTTAGTCATTCTGGACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

16.94

Weight (kDa)

9.27

Isoelectric Point (pI)

55.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 441
AccBSI CCGCTC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 428
AciI CCGC 3 cut(s) 36, 39, 233
AcoI YGGCCR 2 cut(s) 27, 33
AcsI RAATTY 2 cut(s) 176, 272
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 226
AgsI TTSAA 2 cut(s) 136, 277
Alw26I GTCTC 1 cut(s) 384
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 2 cut(s) 27, 33
ApoI RAATTY 2 cut(s) 176, 272
Asp718I GGTACC 1 cut(s) 441
AspLEI GCGC 1 cut(s) 338
AspS9I GGNCC 1 cut(s) 458
AsuHPI GGTGA 1 cut(s) 383
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 1 cut(s) 458
BaeI ACNNNNGTAYC 1 cut(s) 433
BalI TGGCCA 1 cut(s) 29
BanI GGYRCC 1 cut(s) 441
BauI CACGAG 1 cut(s) 19
BccI CCATC 3 cut(s) 62, 199, 400
BceAI ACGGC 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 384
BfmI CTRYAG 1 cut(s) 267
BfoI RGCGCY 1 cut(s) 339
BglII AGATCT 1 cut(s) 263
BisI GCNGC 2 cut(s) 36, 39
BlsI GCNGC 2 cut(s) 37, 40
Bme18I GGWCC 1 cut(s) 458
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 1 cut(s) 443
BmsI GCATC 2 cut(s) 220, 364
BplI GAGNNNNNCTC 2 cut(s) 90, 122
BsaJI CCNNGG 2 cut(s) 30, 435
Bsc4I CCNNNNNNNGG 1 cut(s) 226
BseDI CCNNGG 2 cut(s) 30, 435
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMII CTCAG 1 cut(s) 184
BseRI GAGGAG 1 cut(s) 33
BseX3I CGGCCG 1 cut(s) 33
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 29, 35
BshNI GGYRCC 1 cut(s) 441
BsiEI CGRYCG 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 329
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 1 cut(s) 384
BsmBI CGTCTC 1 cut(s) 384
BsnI GGCC 2 cut(s) 29, 35
BsoBI CYCGRG 1 cut(s) 329
Bsp143I GATC 1 cut(s) 263
BspACI CCGC 3 cut(s) 36, 39, 233
BspANI GGCC 2 cut(s) 29, 35
BspCNI CTCAG 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 441
BsrBI CCGCTC 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 30, 435
BssMI GATC 1 cut(s) 263
BssSI CACGAG 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 19
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 2 cut(s) 170, 446
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 235
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 1 cut(s) 338
BstKTI GATC 1 cut(s) 266
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 1 cut(s) 263
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 2 cut(s) 35, 401
BstNSI RCATGY 1 cut(s) 66
BstSFI CTRYAG 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 263
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 1 cut(s) 263
BstZI CGGCCG 1 cut(s) 33
BsuRI GGCC 2 cut(s) 29, 35
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 3 cut(s) 43, 419, 437
BtsCI GGATG 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 64
CfoI GCGC 1 cut(s) 338
Cfr13I GGNCC 1 cut(s) 458
CseI GACGC 1 cut(s) 366
Csp6I GTAC 1 cut(s) 442
CviAII CATG 3 cut(s) 25, 63, 352
CviJI RGCY 7 cut(s) 29, 35, 91, 105, 312, 343, 434
CviKI_1 RGCY 7 cut(s) 29, 35, 91, 105, 312, 343, 434
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 2 cut(s) 170, 446
DpnI GATC 1 cut(s) 265
DpnII GATC 1 cut(s) 263
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 2 cut(s) 27, 33
EagI CGGCCG 1 cut(s) 33
EclXI CGGCCG 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 458
Eco52I CGGCCG 1 cut(s) 33
Eco88I CYCGRG 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 272
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 384
FaeI CATG 3 cut(s) 28, 66, 355
FaiI YATR 6 cut(s) 8, 26, 64, 95, 353, 359
FatI CATG 3 cut(s) 24, 62, 351
FauI CCCGC 1 cut(s) 226
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 2 cut(s) 36, 39
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 2 cut(s) 36, 39
GlaI GCGC 1 cut(s) 337
GluI GCNGC 2 cut(s) 36, 39
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 2 cut(s) 29, 35
HgaI GACGC 1 cut(s) 366
HhaI GCGC 1 cut(s) 338
Hin1II CATG 3 cut(s) 28, 66, 355
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HincII GTYRAC 1 cut(s) 429
HindII GTYRAC 1 cut(s) 429
HinfI GANTC 1 cut(s) 303
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 429
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 2 cut(s) 109, 456
Hpy8I GTNNAC 1 cut(s) 429
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 66, 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 401
HpyF3I CTNAG 2 cut(s) 170, 446
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 3 cut(s) 28, 66, 355
HspAI GCGC 1 cut(s) 336
KpnI GGTACC 1 cut(s) 445
Kzo9I GATC 1 cut(s) 263
LmnI GCTCC 2 cut(s) 46, 56
LpnPI CCDG 4 cut(s) 301, 332, 381, 441
LweI GCATC 2 cut(s) 220, 364
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 2 cut(s) 160, 236
MalI GATC 1 cut(s) 265
MbiI CCGCTC 1 cut(s) 41
MboI GATC 1 cut(s) 263
MflI RGATCY 1 cut(s) 263
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 3 cut(s) 67, 176, 272
MluNI TGGCCA 1 cut(s) 29
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 5 cut(s) 54, 92, 116, 325, 393
Mox20I TGGCCA 1 cut(s) 29
MscI TGGCCA 1 cut(s) 29
MseI TTAA 1 cut(s) 463
MslI CAYNNNNRTG 2 cut(s) 23, 356
Msp20I TGGCCA 1 cut(s) 29
MwoI GCNNNNNNNGC 2 cut(s) 35, 401
NdeII GATC 1 cut(s) 263
NlaIII CATG 3 cut(s) 28, 66, 355
NlaIV GGNNCC 1 cut(s) 443
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 236
NspI RCATGY 1 cut(s) 66
PaeI GCATGC 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 329
PfeI GAWTC 1 cut(s) 303
PkrI GCNGC 2 cut(s) 37, 40
PspN4I GGNNCC 1 cut(s) 443
PspPI GGNCC 1 cut(s) 458
PspXI VCTCGAGB 1 cut(s) 329
PsuI RGATCY 1 cut(s) 263
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 2 cut(s) 23, 356
SalI GTCGAC 1 cut(s) 427
SaqAI TTAA 1 cut(s) 463
SatI GCNGC 2 cut(s) 36, 39
Sau3AI GATC 1 cut(s) 263
Sau96I GGNCC 1 cut(s) 458
SetI ASST 6 cut(s) 167, 243, 248, 292, 447, 463
SfaNI GCATC 2 cut(s) 220, 364
SfcI CTRYAG 1 cut(s) 267
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 1 cut(s) 458
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 2 cut(s) 23, 356
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SphI GCATGC 1 cut(s) 66
Sse9I AATT 3 cut(s) 67, 176, 272
SsiI CCGC 3 cut(s) 36, 39, 233
SspI AATATT 1 cut(s) 139
StyI CCWWGG 1 cut(s) 435
TaiI ACGT 1 cut(s) 243
TaqI TCGA 3 cut(s) 110, 330, 428
TasI AATT 3 cut(s) 67, 176, 272
TauI GCSGC 2 cut(s) 38, 41
TfiI GAWTC 1 cut(s) 303
Tru1I TTAA 1 cut(s) 463
Tru9I TTAA 1 cut(s) 463
TseFI GTSAC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 458
XapI RAATTY 2 cut(s) 176, 272
XceI RCATGY 1 cut(s) 66
XhoI CTCGAG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.