Rmu_sc0000354.1_g000004

L-tryptophan--pyruvate aminotransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000354.1
OG0007287
Physical Location & Seq
Forward (+)
16595 .. 19061
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000354.1_g000004.1.cds

Sequence Viewer

Length: 1344 bp
atgcaccctctcaacactgagctatatataatagagaagcatgaacgaggagaactaatactgagagcattgtcaatccaaaatcaacctattcgtgttcttagagaaggagacaaaatgtgcggcaccactgatcagaaccctaaccctgcaacccctaacaaaactccgtcagctaatggcaatggcacaacaaggtctagcgtttcacctgacactgtcctcaatctggaacagggtgacccaacaatgttcgaaccgtactggaaaaagatgggggacaagggtacgatggtgatctccggcagccagtggatgagctatgtcagcgacttcaccaacatctgcttttttatggagccagaatttgagggtgcggttagaagacttcatcggacggttgggaatgctgtggtggacggccgtcacattgtggtggggacaggctccacgcagctctaccaggcggcgctgtatgctctcacttctcctggtggggcggagccgatcagtgtcgtgtctgccgctccttactactcgtcatatccagacgagacagactatctccgttcagggctttacagatgggcaggtgatgcgtatgcctttgataagaagggacatggagattacatagaggtggtgaacactccaaacaacccagatggtactaatcggaaagccgtggtgaaatacagaccagatgatcaagttcaagggaagcttattcatgaccttgcctactattggcctcaatacactgccattactgctcctctcgatgaagacattatgtacttcacattttcaaaaagcacgggacatgctggttcccgtattggatgggctgtcgtgagggacaaagagattgctaagaagatggctaaattcgtagagctgagtacacttggtgtgtccaaagtttctcagcaccgagctgcaaagttaatggaagtgatttgtgacggctatcaaaattgcaaggccaaaaactctgagctcttctttgagcactgccagaacctcatggctcaaaggtgggagaggctgagagaagtggtcgagcgcagtgaaattttcagtctgcccaagtatcccaaagagtactgcctcttttctggagaattcaatgaaccacatccagcatttgcgtggttgaagtgcaaggaagacatagacttggagaagtttataagaggatgcatcaaagtgcaaggaagaaccgggaggaagtttgggagtgatcaaaagtatttaaggcttagcatgctgagcaaggatgaggttttcgaccagttcttggagagattgtcaactattaaagccattagtaatgggtattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004021 GO:0004838 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006725 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0008483 GO:0008793 GO:0009058 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009607 GO:0009617 GO:0009628 GO:0009629 GO:0009630 GO:0009639 GO:0009641 GO:0009653 GO:0009683 GO:0009684 GO:0009719 GO:0009723 GO:0009725 GO:0009791 GO:0009850 GO:0009851 GO:0009888 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009987 GO:0010015 GO:0010033 GO:0010073 GO:0010074 GO:0010078 GO:0010087 GO:0010326 GO:0010817 GO:0016053 GO:0016740 GO:0016769 GO:0018130 GO:0019438 GO:0019752 GO:0019827 GO:0019842 GO:0022414 GO:0022622 GO:0030170 GO:0032501 GO:0032502 GO:0032787 GO:0034641 GO:0034754 GO:0036094 GO:0042221 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043436 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0046483 GO:0047312 GO:0047635 GO:0048037 GO:0048364 GO:0048366 GO:0048367 GO:0048438 GO:0048467 GO:0048507 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050048 GO:0050362 GO:0050662 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0070279 GO:0070529 GO:0070546 GO:0070547 GO:0070548 GO:0071704 GO:0072330 GO:0080097 GO:0080098 GO:0080099 GO:0080100 GO:0080130 GO:0090567 GO:0097159 GO:0098542 GO:0098727 GO:0099402 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
Alliinase_C

Protein Analysis

447

Amino Acids

50.62

Weight (kDa)

7.13

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1193
AarI CACCTGC 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 125
AccB7I CCANNNNNTGG 1 cut(s) 1300
AccBSI CCGCTC 1 cut(s) 527
AciI CCGC 5 cut(s) 123, 377, 467, 500, 525
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 4 cut(s) 365, 887, 1074, 1124
AdeI CACNNNGTG 2 cut(s) 433, 911
AfaI GTAC 6 cut(s) 263, 289, 670, 797, 904, 1106
AfiI CCNNNNNNNGG 3 cut(s) 229, 747, 1300
AgsI TTSAA 4 cut(s) 716, 810, 1129, 1159
AjnI CCWGG 2 cut(s) 462, 490
AluBI AGCT 8 cut(s) 22, 176, 321, 457, 724, 898, 938, 1000
AluI AGCT 8 cut(s) 22, 176, 321, 457, 724, 898, 938, 1000
Alw21I GWGCWC 2 cut(s) 1002, 1014
Alw26I GTCTC 2 cut(s) 105, 548
AoxI GGCC 3 cut(s) 421, 749, 984
ApeKI GCWGC 3 cut(s) 306, 454, 938
ApoI RAATTY 4 cut(s) 365, 887, 1074, 1124
AspLEI GCGC 2 cut(s) 472, 1068
AsuC2I CCSGG 1 cut(s) 1225
AsuHPI GGTGA 7 cut(s) 201, 251, 307, 328, 605, 655, 700
AsuII TTCGAA 1 cut(s) 255
BaeI ACNNNNGTAYC 2 cut(s) 279, 312
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 1002
BbsI GAAGAC 3 cut(s) 391, 792, 1176
Bbv12I GWGCWC 2 cut(s) 1002, 1014
BbvI GCAGC 3 cut(s) 318, 466, 925
BccI CCATC 6 cut(s) 268, 286, 579, 659, 837, 874
BceAI ACGGC 4 cut(s) 408, 436, 668, 982
BciT130I CCWGG 2 cut(s) 464, 492
BciVI GTATCC 1 cut(s) 1104
BclI TGATCA 3 cut(s) 133, 706, 1243
BcnI CCSGG 1 cut(s) 1225
BcoDI GTCTC 2 cut(s) 105, 548
BfaI CTAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 581
BfuI GTATCC 1 cut(s) 1104
BisI GCNGC 6 cut(s) 124, 307, 455, 468, 525, 939
BlpI GCTNAGC 2 cut(s) 1262, 1271
BlsI GCNGC 6 cut(s) 125, 308, 456, 469, 526, 940
BmcAI AGTACT 1 cut(s) 1106
Bme1390I CCNGG 3 cut(s) 464, 492, 1225
BmiI GGNNCC 5 cut(s) 127, 360, 448, 504, 832
BmrFI CCNGG 3 cut(s) 464, 492, 1225
BmsI GCATC 3 cut(s) 586, 1190, 1212
BoxI GACNNNNGTC 1 cut(s) 423
BpiI GAAGAC 3 cut(s) 391, 792, 1176
BpmI CTGGAG 1 cut(s) 1140
Bpu1102I GCTNAGC 2 cut(s) 1262, 1271
Bpu14I TTCGAA 1 cut(s) 255
BpuMI CCSGG 1 cut(s) 1225
BsaBI GATNNNNATC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 684
BsaXI ACNNNNNCTCC 2 cut(s) 1295, 1325
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 747, 1300
Bse1I ACTGG 3 cut(s) 269, 310, 1294
Bse3DI GCAATG 1 cut(s) 190
Bse8I GATNNNNATC 1 cut(s) 296
BseBI CCWGG 2 cut(s) 464, 492
BseDI CCNNGG 1 cut(s) 684
BseGI GGATG 5 cut(s) 321, 848, 1138, 1205, 1285
BseJI GATNNNNATC 1 cut(s) 296
BseLI CCNNNNNNNGG 3 cut(s) 229, 747, 1300
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 7 cut(s) 9, 53, 890, 941, 987, 1040, 1262
BseNI ACTGG 3 cut(s) 269, 310, 1294
BseRI GAGGAG 2 cut(s) 63, 765
BseX3I CGGCCG 1 cut(s) 421
BseXI GCAGC 3 cut(s) 318, 466, 925
Bsh1285I CGRYCG 1 cut(s) 424
BshFI GGCC 3 cut(s) 423, 751, 986
BshNI GGYRCC 1 cut(s) 125
BsiEI CGRYCG 1 cut(s) 424
BsiHKAI GWGCWC 2 cut(s) 1002, 1014
BsiSI CCGG 2 cut(s) 303, 1224
BslFI GGGAC 5 cut(s) 293, 454, 633, 834, 872
BslI CCNNNNNNNGG 3 cut(s) 229, 747, 1300
BsmAI GTCTC 2 cut(s) 105, 548
BsmFI GGGAC 5 cut(s) 293, 454, 633, 834, 872
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 3 cut(s) 423, 751, 986
Bsp119I TTCGAA 1 cut(s) 255
Bsp1286I GDGCHC 2 cut(s) 1002, 1014
Bsp143I GATC 5 cut(s) 133, 297, 507, 706, 1243
Bsp1720I GCTNAGC 2 cut(s) 1262, 1271
BspACI CCGC 5 cut(s) 123, 377, 467, 500, 525
BspANI GGCC 3 cut(s) 423, 751, 986
BspCNI CTCAG 7 cut(s) 10, 54, 891, 940, 988, 1041, 1263
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 5 cut(s) 127, 360, 448, 504, 832
BspMI ACCTGC 1 cut(s) 581
BspQI GCTCTTC 1 cut(s) 1007
BspT104I TTCGAA 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 125
BsrBI CCGCTC 1 cut(s) 527
BsrDI GCAATG 1 cut(s) 190
BsrI ACTGG 3 cut(s) 269, 310, 1294
BssECI CCNNGG 1 cut(s) 684
BssMI GATC 5 cut(s) 133, 297, 507, 706, 1243
Bst2UI CCWGG 2 cut(s) 464, 492
Bst4CI ACNGT 3 cut(s) 220, 261, 400
Bst6I CTCTTC 1 cut(s) 1007
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 1268
BstDSI CCRYGG 1 cut(s) 684
BstEII GGTNACC 1 cut(s) 239
BstF5I GGATG 5 cut(s) 321, 848, 1138, 1205, 1285
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 2 cut(s) 472, 1068
BstKTI GATC 5 cut(s) 136, 300, 510, 709, 1246
BstMAI GTCTC 2 cut(s) 105, 548
BstMBI GATC 5 cut(s) 133, 297, 507, 706, 1243
BstMCI CGRYCG 1 cut(s) 424
BstMWI GCNNNNNNNGC 6 cut(s) 327, 476, 596, 770, 1267, 1272
BstNI CCWGG 2 cut(s) 464, 492
BstNSI RCATGY 2 cut(s) 827, 1270
BstPAI GACNNNNGTC 1 cut(s) 423
BstPI GGTNACC 1 cut(s) 239
BstSCI CCNGG 3 cut(s) 462, 490, 1223
BstV1I GCAGC 3 cut(s) 318, 466, 925
BstV2I GAAGAC 3 cut(s) 391, 792, 1176
BstZI CGGCCG 1 cut(s) 421
BsuI GTATCC 1 cut(s) 1104
BsuRI GGCC 3 cut(s) 423, 751, 986
BtgI CCRYGG 1 cut(s) 684
BtsCI GGATG 5 cut(s) 321, 848, 1138, 1205, 1285
BtsI GCAGTG 3 cut(s) 759, 1012, 1075
BtsIMutI CAGTG 8 cut(s) 15, 129, 216, 317, 517, 759, 1012, 1075
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 1 cut(s) 1268
CciI TCATGA 1 cut(s) 730
CfoI GCGC 2 cut(s) 472, 1068
Csp6I GTAC 6 cut(s) 262, 288, 669, 796, 903, 1105
CviAII CATG 6 cut(s) 41, 623, 731, 824, 1027, 1267
CviQI GTAC 6 cut(s) 262, 288, 669, 796, 903, 1105
DpnI GATC 5 cut(s) 135, 299, 509, 708, 1245
DpnII GATC 5 cut(s) 133, 297, 507, 706, 1243
DraIII CACNNNGTG 2 cut(s) 433, 911
EaeI YGGCCR 1 cut(s) 421
EagI CGGCCG 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 1007
EarI CTCTTC 1 cut(s) 1007
EciI GGCGGA 1 cut(s) 515
Ecl136II GAGCTC 1 cut(s) 1000
EclXI CGGCCG 1 cut(s) 421
Eco24I GRGCYC 1 cut(s) 1002
Eco52I CGGCCG 1 cut(s) 421
Eco53kI GAGCTC 1 cut(s) 1000
Eco91I GGTNACC 1 cut(s) 239
EcoICRI GAGCTC 1 cut(s) 1000
EcoO65I GGTNACC 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 1124
EcoRII CCWGG 2 cut(s) 462, 490
EcoT22I ATGCAT 1 cut(s) 1205
EcoT38I GRGCYC 1 cut(s) 1002
FaeI CATG 6 cut(s) 44, 626, 734, 827, 1030, 1270
FalI AAGNNNNNCTT 2 cut(s) 708, 740
FaqI GGGAC 5 cut(s) 293, 454, 633, 834, 872
FatI CATG 6 cut(s) 40, 622, 730, 823, 1026, 1266
FbaI TGATCA 3 cut(s) 133, 706, 1243
Fnu4HI GCNGC 6 cut(s) 124, 307, 455, 468, 525, 939
FokI GGATG 5 cut(s) 328, 855, 1125, 1212, 1292
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 6 cut(s) 124, 307, 455, 468, 525, 939
FspBI CTAG 1 cut(s) 201
GlaI GCGC 2 cut(s) 471, 1067
GluI GCNGC 6 cut(s) 124, 307, 455, 468, 525, 939
GsuI CTGGAG 1 cut(s) 1140
HaeII RGCGCY 1 cut(s) 473
HaeIII GGCC 3 cut(s) 423, 751, 986
HapII CCGG 2 cut(s) 303, 1224
HhaI GCGC 2 cut(s) 472, 1068
Hin1II CATG 6 cut(s) 44, 626, 734, 827, 1030, 1270
Hin6I GCGC 2 cut(s) 470, 1066
HinP1I GCGC 2 cut(s) 470, 1066
HincII GTYRAC 1 cut(s) 1314
HindII GTYRAC 1 cut(s) 1314
HindIII AAGCTT 1 cut(s) 722
HpaII CCGG 2 cut(s) 303, 1224
HphI GGTGA 7 cut(s) 201, 251, 307, 328, 605, 655, 700
Hpy166II GTNNAC 4 cut(s) 418, 646, 905, 1314
Hpy188I TCNGA 4 cut(s) 138, 396, 678, 997
Hpy188III TCNNGA 6 cut(s) 230, 548, 731, 779, 853, 1119
Hpy8I GTNNAC 4 cut(s) 418, 646, 905, 1314
HpyAV CCTTC 2 cut(s) 101, 610
HpyCH4III ACNGT 3 cut(s) 220, 261, 400
HpyCH4V TGCA 7 cut(s) 4, 152, 941, 981, 1164, 1203, 1213
HpyF10VI GCNNNNNNNGC 6 cut(s) 327, 476, 596, 770, 1267, 1272
Hsp92II CATG 6 cut(s) 44, 626, 734, 827, 1030, 1270
HspAI GCGC 2 cut(s) 470, 1066
Ksp22I TGATCA 3 cut(s) 133, 706, 1243
Kzo9I GATC 5 cut(s) 133, 297, 507, 706, 1243
LguI GCTCTTC 1 cut(s) 1007
LmnI GCTCC 5 cut(s) 358, 452, 502, 532, 778
Lsp1109I GCAGC 3 cut(s) 318, 466, 925
LweI GCATC 3 cut(s) 586, 1190, 1212
MaeI CTAG 1 cut(s) 201
MaeIII GTNAC 3 cut(s) 239, 425, 962
MalI GATC 5 cut(s) 135, 299, 509, 708, 1245
MbiI CCGCTC 1 cut(s) 527
MboI GATC 5 cut(s) 133, 297, 507, 706, 1243
MboII GAAGA 6 cut(s) 396, 797, 889, 994, 1181, 1230
MhlI GDGCHC 2 cut(s) 1002, 1014
MluCI AATT 5 cut(s) 365, 887, 976, 1074, 1124
Mph1103I ATGCAT 1 cut(s) 1205
MseI TTAA 4 cut(s) 947, 1256, 1320, 1342
MslI CAYNNNNRTG 4 cut(s) 434, 638, 1150, 1208
MspI CCGG 2 cut(s) 303, 1224
MspR9I CCNGG 3 cut(s) 464, 492, 1225
Mva1269I GAATGC 1 cut(s) 412
MvaI CCWGG 2 cut(s) 464, 492
MwoI GCNNNNNNNGC 6 cut(s) 327, 476, 596, 770, 1267, 1272
NciI CCSGG 1 cut(s) 1225
NdeII GATC 5 cut(s) 133, 297, 507, 706, 1243
NlaIII CATG 6 cut(s) 44, 626, 734, 827, 1030, 1270
NlaIV GGNNCC 5 cut(s) 127, 360, 448, 504, 832
NmuCI GTSAC 3 cut(s) 239, 425, 962
NsiI ATGCAT 1 cut(s) 1205
NspI RCATGY 2 cut(s) 827, 1270
NspV TTCGAA 1 cut(s) 255
PaeI GCATGC 1 cut(s) 1270
PagI TCATGA 1 cut(s) 730
PaqCI CACCTGC 1 cut(s) 581
PciSI GCTCTTC 1 cut(s) 1007
PctI GAATGC 1 cut(s) 412
PflFI GACNNNGTC 1 cut(s) 218
PflMI CCANNNNNTGG 1 cut(s) 1300
PkrI GCNGC 6 cut(s) 125, 308, 456, 469, 526, 940
PshAI GACNNNNGTC 1 cut(s) 423
PsiI TTATAA 1 cut(s) 1193
Psp124BI GAGCTC 1 cut(s) 1002
Psp6I CCWGG 2 cut(s) 462, 490
PspEI GGTNACC 1 cut(s) 239
PspGI CCWGG 2 cut(s) 462, 490
PspN4I GGNNCC 5 cut(s) 127, 360, 448, 504, 832
PsyI GACNNNGTC 1 cut(s) 218
RsaI GTAC 6 cut(s) 263, 289, 670, 797, 904, 1106
RsaNI GTAC 6 cut(s) 262, 288, 669, 796, 903, 1105
RseI CAYNNNNRTG 4 cut(s) 434, 638, 1150, 1208
SacI GAGCTC 1 cut(s) 1002
SapI GCTCTTC 1 cut(s) 1007
SaqAI TTAA 4 cut(s) 947, 1256, 1320, 1342
SatI GCNGC 6 cut(s) 124, 307, 455, 468, 525, 939
Sau3AI GATC 5 cut(s) 133, 297, 507, 706, 1243
ScaI AGTACT 1 cut(s) 1106
ScrFI CCNGG 3 cut(s) 464, 492, 1225
SduI GDGCHC 2 cut(s) 1002, 1014
SfaNI GCATC 3 cut(s) 586, 1190, 1212
SfuI TTCGAA 1 cut(s) 255
SmiMI CAYNNNNRTG 4 cut(s) 434, 638, 1150, 1208
SphI GCATGC 1 cut(s) 1270
Sse9I AATT 5 cut(s) 365, 887, 976, 1074, 1124
SsiI CCGC 5 cut(s) 123, 377, 467, 500, 525
SspMI CTAG 1 cut(s) 201
SstI GAGCTC 1 cut(s) 1002
StyD4I CCNGG 3 cut(s) 462, 490, 1223
TaaI ACNGT 3 cut(s) 220, 261, 400
TaqI TCGA 4 cut(s) 255, 780, 1062, 1290
TasI AATT 5 cut(s) 365, 887, 976, 1074, 1124
TatI WGTACW 3 cut(s) 795, 902, 1104
TauI GCSGC 3 cut(s) 126, 470, 527
Tru1I TTAA 4 cut(s) 947, 1256, 1320, 1342
Tru9I TTAA 4 cut(s) 947, 1256, 1320, 1342
TscAI CASTG 8 cut(s) 22, 136, 223, 317, 517, 766, 1019, 1075
TseFI GTSAC 3 cut(s) 239, 425, 962
TseI GCWGC 3 cut(s) 306, 454, 938
Tsp45I GTSAC 3 cut(s) 239, 425, 962
TspDTI ATGAA 5 cut(s) 57, 380, 719, 798, 1146
TspGWI ACGGA 2 cut(s) 159, 557
TspRI CASTG 8 cut(s) 22, 136, 223, 317, 517, 766, 1019, 1075
Tth111I GACNNNGTC 1 cut(s) 218
Van91I CCANNNNNTGG 1 cut(s) 1300
XapI RAATTY 4 cut(s) 365, 887, 1074, 1124
XceI RCATGY 2 cut(s) 827, 1270
XcmI CCANNNNNNNNNTGG 1 cut(s) 1149
XspI CTAG 1 cut(s) 201
ZrmI AGTACT 1 cut(s) 1106
Zsp2I ATGCAT 1 cut(s) 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.