Rw4G027890

L-tryptophan--pyruvate aminotransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007287
Physical Location & Seq
Forward (+)
54631695 .. 54634051
2357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027890.1

Sequence Viewer

Length: 1227 bp
ATGTGCGGCACCACTGATCAGAACCCTAACCCTGCAACCCCTAACAAAACACCGTCAGCTAATGGCAATGGCACAACAAGGTCTAGCGTTTCACCTGACACTGTCCTCAATCTGGAACAGGGTGACCCAACAATGTTCGAACCGTACTGGAAAAAGATGGGGGACAAGGGTACGATGGTGATCTCCGGCAGCCAGTGGATGAGCTATGTCAGCGACTTCACCAACATCTGCTTTTTTATGGAGCCAGAATTTGAGGGTGCGGTTAGAAGACTTCATCGGACGGTTGGGAATGCTGTGGTGGACGGCCGTCACATTGTGGTGGGGACAGGCTCCACGCAGCTCTACCAGGCGGCACTGTATGCTCTCACTTCTCCTGGTGGGGCGGAGCCGATCAGTGTCGTGTCTGCCGCTCCTTACTACTCGTCATATCCAGACGAGACAGACTATCTCCGTTCAGGGCTTTACAGATGGGCAGGTGATGCGTATGACTTTGATAAGAAGGGACATGGAGATTACATAGAGGTGGTGAACACTCCAAACAACCCAGATGGTACTAATCGGAAAGCCGTGGTGAAATACAGACCAGATGATCAAGTTCAAGGGAAGCTTATTCATGACCTTGCCTACTATTGGCCTCAATACACTGCCATTACTGCTCCTCTCGATGAAGACATTATGTACTTCACATTTTCAAAAAGCACGGGACATGCTGGTTCCCGTATTGGATGGGCTGTCGTGAAGGACAAAGAGATTGCTAAGAAGATGGCTAAATTCGTAGAGCTGAGTACACTTGGTGTGTCCAAAGTTTCTCAGCACCGAGCTGCAAAGTTAATGGGAGTGATTTGTGACGGCTATCAAAATTGCAAGGCCAAAAACTCTGAGCTCTTCTTTGAGCACTGCCAGAACCTCATGGCTCAAAGGTGGGAGAGGCTGACAGAAGTGGTCGAGCGCAGTGAAATTTTCAGTCTGCCCAAGTATCCCAAAGAGTACTGCCTCTTTTCTGGAGAATTCAATGAACCACATCCAGCATTTGCGTGGTTGAAGTGCAAGGAAGACATAGACTTGGAGAAGTTTATAAGAGGATGCATCAAAGTGCAAGGAAGAACCGGGAGGAAGTTTGGGAGTGATCAAAAGTATTTAAGGCTTAGCATGCTGAGCAAGGATGAGGTTTTCGACCAGTTCTTGGAGAGATTGTCGACTATTAAAGCCATTAGTAATGGGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004021 GO:0004838 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006725 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0008483 GO:0008793 GO:0009058 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009607 GO:0009617 GO:0009628 GO:0009629 GO:0009630 GO:0009639 GO:0009641 GO:0009653 GO:0009683 GO:0009684 GO:0009719 GO:0009723 GO:0009725 GO:0009791 GO:0009850 GO:0009851 GO:0009888 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009987 GO:0010015 GO:0010033 GO:0010073 GO:0010074 GO:0010078 GO:0010087 GO:0010326 GO:0010817 GO:0016053 GO:0016740 GO:0016769 GO:0018130 GO:0019438 GO:0019752 GO:0019827 GO:0019842 GO:0022414 GO:0022622 GO:0030170 GO:0032501 GO:0032502 GO:0032787 GO:0034641 GO:0034754 GO:0036094 GO:0042221 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043436 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0046483 GO:0047312 GO:0047635 GO:0048037 GO:0048364 GO:0048366 GO:0048367 GO:0048438 GO:0048467 GO:0048507 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050048 GO:0050362 GO:0050662 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0070279 GO:0070529 GO:0070546 GO:0070547 GO:0070548 GO:0071704 GO:0072330 GO:0080097 GO:0080098 GO:0080099 GO:0080100 GO:0080130 GO:0090567 GO:0097159 GO:0098542 GO:0098727 GO:0099402 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
Alliinase_C

Protein Analysis

408

Amino Acids

45.9

Weight (kDa)

6.72

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alliinase_C PF04864 37 - 401 8.4e-137 Allinase
Aminotran_1_2 PF00155 81 - 398 3.8e-10 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1076
AarI CACCTGC 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 464
AccB1I GGYRCC 1 cut(s) 8
AccB7I CCANNNNNTGG 1 cut(s) 1183
AccBSI CCGCTC 1 cut(s) 410
AccI GTMKAC 1 cut(s) 1196
AciI CCGC 5 cut(s) 6, 260, 350, 383, 408
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 4 cut(s) 248, 770, 957, 1007
AdeI CACNNNGTG 2 cut(s) 316, 794
AfaI GTAC 6 cut(s) 146, 172, 553, 680, 787, 989
AfiI CCNNNNNNNGG 3 cut(s) 112, 630, 1183
AgsI TTSAA 4 cut(s) 599, 693, 1012, 1042
AjnI CCWGG 2 cut(s) 345, 373
AluBI AGCT 7 cut(s) 59, 204, 340, 607, 781, 821, 883
AluI AGCT 7 cut(s) 59, 204, 340, 607, 781, 821, 883
Alw21I GWGCWC 2 cut(s) 885, 897
Alw26I GTCTC 1 cut(s) 431
AoxI GGCC 3 cut(s) 304, 632, 867
ApeKI GCWGC 3 cut(s) 189, 337, 821
ApoI RAATTY 4 cut(s) 248, 770, 957, 1007
AspLEI GCGC 1 cut(s) 951
AsuC2I CCSGG 1 cut(s) 1108
AsuHPI GGTGA 7 cut(s) 84, 134, 190, 211, 488, 538, 583
AsuII TTCGAA 1 cut(s) 138
BaeI ACNNNNGTAYC 2 cut(s) 162, 195
BanI GGYRCC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 885
BbsI GAAGAC 3 cut(s) 274, 675, 1059
Bbv12I GWGCWC 2 cut(s) 885, 897
BbvI GCAGC 3 cut(s) 201, 349, 808
BccI CCATC 6 cut(s) 151, 169, 462, 542, 720, 757
BceAI ACGGC 4 cut(s) 291, 319, 551, 865
BciT130I CCWGG 2 cut(s) 347, 375
BciVI GTATCC 1 cut(s) 987
BclI TGATCA 3 cut(s) 16, 589, 1126
BcnI CCSGG 1 cut(s) 1108
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 464
BfuI GTATCC 1 cut(s) 987
BisI GCNGC 6 cut(s) 7, 190, 338, 351, 408, 822
BlpI GCTNAGC 2 cut(s) 1145, 1154
BlsI GCNGC 6 cut(s) 8, 191, 339, 352, 409, 823
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 3 cut(s) 347, 375, 1108
BmiI GGNNCC 5 cut(s) 10, 243, 331, 387, 715
BmrFI CCNGG 3 cut(s) 347, 375, 1108
BmsI GCATC 3 cut(s) 469, 1073, 1095
BoxI GACNNNNGTC 1 cut(s) 306
BpiI GAAGAC 3 cut(s) 274, 675, 1059
BpmI CTGGAG 1 cut(s) 1023
Bpu1102I GCTNAGC 2 cut(s) 1145, 1154
Bpu14I TTCGAA 1 cut(s) 138
BpuMI CCSGG 1 cut(s) 1108
BsaBI GATNNNNATC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 567
BsaXI ACNNNNNCTCC 2 cut(s) 1178, 1208
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 630, 1183
Bse1I ACTGG 3 cut(s) 152, 193, 1177
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 2 cut(s) 347, 375
BseDI CCNNGG 1 cut(s) 567
BseGI GGATG 5 cut(s) 204, 731, 1021, 1088, 1168
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 3 cut(s) 112, 630, 1183
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 4 cut(s) 773, 824, 870, 1145
BseNI ACTGG 3 cut(s) 152, 193, 1177
BseRI GAGGAG 1 cut(s) 648
BseX3I CGGCCG 1 cut(s) 304
BseXI GCAGC 3 cut(s) 201, 349, 808
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 3 cut(s) 306, 634, 869
BshNI GGYRCC 1 cut(s) 8
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 2 cut(s) 885, 897
BsiSI CCGG 2 cut(s) 186, 1107
BslFI GGGAC 4 cut(s) 176, 337, 516, 717
BslI CCNNNNNNNGG 3 cut(s) 112, 630, 1183
BsmAI GTCTC 1 cut(s) 431
BsmFI GGGAC 4 cut(s) 176, 337, 516, 717
BsmI GAATGC 1 cut(s) 295
BsnI GGCC 3 cut(s) 306, 634, 869
Bsp119I TTCGAA 1 cut(s) 138
Bsp1286I GDGCHC 2 cut(s) 885, 897
Bsp143I GATC 5 cut(s) 16, 180, 390, 589, 1126
Bsp1720I GCTNAGC 2 cut(s) 1145, 1154
BspACI CCGC 5 cut(s) 6, 260, 350, 383, 408
BspANI GGCC 3 cut(s) 306, 634, 869
BspCNI CTCAG 4 cut(s) 774, 823, 871, 1146
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 5 cut(s) 10, 243, 331, 387, 715
BspMI ACCTGC 1 cut(s) 464
BspQI GCTCTTC 1 cut(s) 890
BspT104I TTCGAA 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 8
BsrBI CCGCTC 1 cut(s) 410
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 3 cut(s) 152, 193, 1177
BssECI CCNNGG 1 cut(s) 567
BssMI GATC 5 cut(s) 16, 180, 390, 589, 1126
Bst2UI CCWGG 2 cut(s) 347, 375
Bst4CI ACNGT 5 cut(s) 54, 103, 144, 283, 357
Bst6I CTCTTC 1 cut(s) 890
BstAPI GCANNNNNTGC 2 cut(s) 359, 479
BstBI TTCGAA 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 1151
BstDEI CTNAG 6 cut(s) 756, 782, 810, 879, 1145, 1154
BstDSI CCRYGG 1 cut(s) 567
BstEII GGTNACC 1 cut(s) 122
BstF5I GGATG 5 cut(s) 204, 731, 1021, 1088, 1168
BstHHI GCGC 1 cut(s) 951
BstKTI GATC 5 cut(s) 19, 183, 393, 592, 1129
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 5 cut(s) 16, 180, 390, 589, 1126
BstMCI CGRYCG 1 cut(s) 307
BstMWI GCNNNNNNNGC 6 cut(s) 210, 359, 479, 653, 1150, 1155
BstNI CCWGG 2 cut(s) 347, 375
BstNSI RCATGY 2 cut(s) 710, 1153
BstPAI GACNNNNGTC 1 cut(s) 306
BstPI GGTNACC 1 cut(s) 122
BstSCI CCNGG 3 cut(s) 345, 373, 1106
BstV1I GCAGC 3 cut(s) 201, 349, 808
BstV2I GAAGAC 3 cut(s) 274, 675, 1059
BstZI CGGCCG 1 cut(s) 304
BsuI GTATCC 1 cut(s) 987
BsuRI GGCC 3 cut(s) 306, 634, 869
BtgI CCRYGG 1 cut(s) 567
BtsCI GGATG 5 cut(s) 204, 731, 1021, 1088, 1168
BtsI GCAGTG 3 cut(s) 642, 895, 958
BtsIMutI CAGTG 8 cut(s) 12, 99, 200, 353, 400, 642, 895, 958
BveI ACCTGC 1 cut(s) 464
Cac8I GCNNGC 1 cut(s) 1151
CciI TCATGA 1 cut(s) 613
CfoI GCGC 1 cut(s) 951
Csp6I GTAC 6 cut(s) 145, 171, 552, 679, 786, 988
CviAII CATG 5 cut(s) 506, 614, 707, 910, 1150
CviQI GTAC 6 cut(s) 145, 171, 552, 679, 786, 988
DdeI CTNAG 6 cut(s) 756, 782, 810, 879, 1145, 1154
DpnI GATC 5 cut(s) 18, 182, 392, 591, 1128
DpnII GATC 5 cut(s) 16, 180, 390, 589, 1126
DraIII CACNNNGTG 2 cut(s) 316, 794
EaeI YGGCCR 1 cut(s) 304
EagI CGGCCG 1 cut(s) 304
Eam1104I CTCTTC 1 cut(s) 890
EarI CTCTTC 1 cut(s) 890
EciI GGCGGA 1 cut(s) 398
Ecl136II GAGCTC 1 cut(s) 883
EclXI CGGCCG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 885
Eco52I CGGCCG 1 cut(s) 304
Eco53kI GAGCTC 1 cut(s) 883
Eco91I GGTNACC 1 cut(s) 122
EcoICRI GAGCTC 1 cut(s) 883
EcoO65I GGTNACC 1 cut(s) 122
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 2 cut(s) 345, 373
EcoT22I ATGCAT 1 cut(s) 1088
EcoT38I GRGCYC 1 cut(s) 885
FaeI CATG 5 cut(s) 509, 617, 710, 913, 1153
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 4 cut(s) 176, 337, 516, 717
FatI CATG 5 cut(s) 505, 613, 706, 909, 1149
FbaI TGATCA 3 cut(s) 16, 589, 1126
FblI GTMKAC 1 cut(s) 1196
Fnu4HI GCNGC 6 cut(s) 7, 190, 338, 351, 408, 822
FokI GGATG 5 cut(s) 211, 738, 1008, 1095, 1175
FriOI GRGCYC 1 cut(s) 885
Fsp4HI GCNGC 6 cut(s) 7, 190, 338, 351, 408, 822
FspBI CTAG 1 cut(s) 84
GlaI GCGC 1 cut(s) 950
GluI GCNGC 6 cut(s) 7, 190, 338, 351, 408, 822
GsuI CTGGAG 1 cut(s) 1023
HaeIII GGCC 3 cut(s) 306, 634, 869
HapII CCGG 2 cut(s) 186, 1107
HhaI GCGC 1 cut(s) 951
Hin1II CATG 5 cut(s) 509, 617, 710, 913, 1153
Hin6I GCGC 1 cut(s) 949
HinP1I GCGC 1 cut(s) 949
HincII GTYRAC 1 cut(s) 1197
HindII GTYRAC 1 cut(s) 1197
HindIII AAGCTT 1 cut(s) 605
HpaII CCGG 2 cut(s) 186, 1107
HphI GGTGA 7 cut(s) 84, 134, 190, 211, 488, 538, 583
Hpy166II GTNNAC 4 cut(s) 301, 529, 788, 1197
Hpy188I TCNGA 4 cut(s) 21, 279, 561, 880
Hpy188III TCNNGA 6 cut(s) 113, 431, 614, 662, 736, 1002
Hpy8I GTNNAC 4 cut(s) 301, 529, 788, 1197
HpyAV CCTTC 2 cut(s) 493, 733
HpyCH4III ACNGT 5 cut(s) 54, 103, 144, 283, 357
HpyCH4V TGCA 6 cut(s) 35, 824, 864, 1047, 1086, 1096
HpyF10VI GCNNNNNNNGC 6 cut(s) 210, 359, 479, 653, 1150, 1155
HpyF3I CTNAG 6 cut(s) 756, 782, 810, 879, 1145, 1154
Hsp92II CATG 5 cut(s) 509, 617, 710, 913, 1153
HspAI GCGC 1 cut(s) 949
Ksp22I TGATCA 3 cut(s) 16, 589, 1126
Kzo9I GATC 5 cut(s) 16, 180, 390, 589, 1126
LguI GCTCTTC 1 cut(s) 890
LmnI GCTCC 5 cut(s) 241, 335, 385, 415, 661
Lsp1109I GCAGC 3 cut(s) 201, 349, 808
LweI GCATC 3 cut(s) 469, 1073, 1095
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 3 cut(s) 122, 308, 845
MalI GATC 5 cut(s) 18, 182, 392, 591, 1128
MbiI CCGCTC 1 cut(s) 410
MboI GATC 5 cut(s) 16, 180, 390, 589, 1126
MboII GAAGA 6 cut(s) 279, 680, 772, 877, 1064, 1113
MhlI GDGCHC 2 cut(s) 885, 897
MluCI AATT 5 cut(s) 248, 770, 859, 957, 1007
Mph1103I ATGCAT 1 cut(s) 1088
MseI TTAA 4 cut(s) 830, 1139, 1203, 1225
MslI CAYNNNNRTG 4 cut(s) 317, 521, 1033, 1091
MspI CCGG 2 cut(s) 186, 1107
MspR9I CCNGG 3 cut(s) 347, 375, 1108
Mva1269I GAATGC 1 cut(s) 295
MvaI CCWGG 2 cut(s) 347, 375
MwoI GCNNNNNNNGC 6 cut(s) 210, 359, 479, 653, 1150, 1155
NciI CCSGG 1 cut(s) 1108
NdeII GATC 5 cut(s) 16, 180, 390, 589, 1126
NlaIII CATG 5 cut(s) 509, 617, 710, 913, 1153
NlaIV GGNNCC 5 cut(s) 10, 243, 331, 387, 715
NmuCI GTSAC 3 cut(s) 122, 308, 845
NsiI ATGCAT 1 cut(s) 1088
NspI RCATGY 2 cut(s) 710, 1153
NspV TTCGAA 1 cut(s) 138
PaeI GCATGC 1 cut(s) 1153
PagI TCATGA 1 cut(s) 613
PaqCI CACCTGC 1 cut(s) 464
PciSI GCTCTTC 1 cut(s) 890
PctI GAATGC 1 cut(s) 295
PflFI GACNNNGTC 1 cut(s) 101
PflMI CCANNNNNTGG 1 cut(s) 1183
PkrI GCNGC 6 cut(s) 8, 191, 339, 352, 409, 823
PshAI GACNNNNGTC 1 cut(s) 306
PsiI TTATAA 1 cut(s) 1076
Psp124BI GAGCTC 1 cut(s) 885
Psp6I CCWGG 2 cut(s) 345, 373
PspEI GGTNACC 1 cut(s) 122
PspGI CCWGG 2 cut(s) 345, 373
PspN4I GGNNCC 5 cut(s) 10, 243, 331, 387, 715
PsyI GACNNNGTC 1 cut(s) 101
RsaI GTAC 6 cut(s) 146, 172, 553, 680, 787, 989
RsaNI GTAC 6 cut(s) 145, 171, 552, 679, 786, 988
RseI CAYNNNNRTG 4 cut(s) 317, 521, 1033, 1091
SacI GAGCTC 1 cut(s) 885
SalI GTCGAC 1 cut(s) 1195
SapI GCTCTTC 1 cut(s) 890
SaqAI TTAA 4 cut(s) 830, 1139, 1203, 1225
SatI GCNGC 6 cut(s) 7, 190, 338, 351, 408, 822
Sau3AI GATC 5 cut(s) 16, 180, 390, 589, 1126
ScaI AGTACT 1 cut(s) 989
ScrFI CCNGG 3 cut(s) 347, 375, 1108
SduI GDGCHC 2 cut(s) 885, 897
SfaNI GCATC 3 cut(s) 469, 1073, 1095
SfuI TTCGAA 1 cut(s) 138
SmiMI CAYNNNNRTG 4 cut(s) 317, 521, 1033, 1091
SphI GCATGC 1 cut(s) 1153
Sse9I AATT 5 cut(s) 248, 770, 859, 957, 1007
SsiI CCGC 5 cut(s) 6, 260, 350, 383, 408
SspMI CTAG 1 cut(s) 84
SstI GAGCTC 1 cut(s) 885
StyD4I CCNGG 3 cut(s) 345, 373, 1106
TaaI ACNGT 5 cut(s) 54, 103, 144, 283, 357
TaqI TCGA 5 cut(s) 138, 663, 945, 1173, 1196
TasI AATT 5 cut(s) 248, 770, 859, 957, 1007
TatI WGTACW 3 cut(s) 678, 785, 987
TauI GCSGC 3 cut(s) 9, 353, 410
Tru1I TTAA 4 cut(s) 830, 1139, 1203, 1225
Tru9I TTAA 4 cut(s) 830, 1139, 1203, 1225
TscAI CASTG 8 cut(s) 19, 106, 200, 360, 400, 649, 902, 958
TseFI GTSAC 3 cut(s) 122, 308, 845
TseI GCWGC 3 cut(s) 189, 337, 821
Tsp45I GTSAC 3 cut(s) 122, 308, 845
TspDTI ATGAA 4 cut(s) 263, 602, 681, 1029
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 8 cut(s) 19, 106, 200, 360, 400, 649, 902, 958
Tth111I GACNNNGTC 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 1183
XapI RAATTY 4 cut(s) 248, 770, 957, 1007
XceI RCATGY 2 cut(s) 710, 1153
XcmI CCANNNNNNNNNTGG 1 cut(s) 1032
XmiI GTMKAC 1 cut(s) 1196
XspI CTAG 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 989
Zsp2I ATGCAT 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.