Rmu_sc0000665.1_g000016

Transcriptional corepressor LEUNIG-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000665.1
OG0008825
Physical Location & Seq
Reverse (-)
80582 .. 83146
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ctgctccgatcccctcttctctctcctctcggccgagagcctctgaggaatgatatggaccggtttgtggatgacggatctttagaggataattttgaatcattcttatcacttgatgatgctgaccctaggggtagagttgctcggcgttcagatgtcagcagcagaggtttcactttttcggaagtcggggttattcctgcaagttcaagtgaagtcgagtgctgtcacttctcagcagatgggaaaacacttgccactggtgggcatgatcgaaaggctgtattgtggtgcacagagtccttggctgtaaagtctacgcttgaagagcattctcagtggataacggatgttcgattcagtcctagtatgtcaaggctagctacatcttccgctgacaaaactgtcagggtttgggatgttgataatcctggctattcacttcatacttttatgggacattctacaactgttatgtcacttgacttccaccctactaaagaggatcttctgtgctcttgtgataacaacagtgagataagatactggagtatcaagaatggtagttgtattggagtttccaagggtggtgcaactcagatgaggtttcaacctcgtcttggaaggatgcttgctgctgcaggtgatactcttgtatccataattgatgtagagaccgcatgttgcacgcttaaattactgggtcataagaaccttgtcaattctgtgtgctgggatccttctggtgagtatctagcctgtgtgagtgatgactcggttagagtatgggcagttggctccagtagcaaatgcgaatgcctttacgagttaagctgttctgacaacaaatttcagacatgcgtcttccatcctacttatccatcattgttggtaatcggctgttatgagacattggaactttggaacatgagcgagaacaagacaatgaccttgcctgcacatgacaagctagtatcttctttggcggcatcatgttctactggcatgttagcttcagctagccatgataagtgcgtgaagctatggaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

350

Amino Acids

38.44

Weight (kDa)

5.49

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 58 - 192 3e-12 G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd PF24782 60 - 263 8e-16 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 62 - 184 1.9e-13 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 62 - 122 4.1e-08 THOC3 beta-propeller domain
EIF3I PF24805 64 - 143 2.3e-06 EIF3I
WD40_WDHD1_1st PF24817 64 - 143 5.6e-11 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 69 - 192 9.3e-26 WDR5 beta-propeller domain
WD40_Prp19 PF24814 69 - 263 7.9e-31 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 70 - 185 9.2e-23 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 70 - 189 4.1e-19 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML PF23409 71 - 190 6.3e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 73 - 184 2.3e-11 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 73 - 183 1.6e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 76 - 143 3.1e-07 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 101 - 192 8.1e-14 CDC20/Fizzy WD40 domain
WDR55 PF24796 105 - 350 2.4e-13 WDR55
WD40 PF00400 106 - 140 1.5e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 111 - 350 1.8e-40 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 122 - 232 2.6e-08 WDR90/POC16, second beta-propeller
EIF3I PF24805 153 - 349 1.8e-07 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 171 - 274 2.7e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 180 - 308 1.2e-10 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 196 - 349 4.1e-25 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 199 - 350 1.2e-14 WDHD1 first WD40 domain
WD40_Prp19 PF24814 199 - 349 2.1e-15 Prp19 WD40 domain
WD40_Gbeta PF25391 201 - 349 7.9e-07 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 214 - 316 3.8e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 229 - 263 7.9e-08 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 241 - 350 2.3e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 260 - 349 6.6e-06 WDR3 first beta-propeller domain
WD40 PF00400 315 - 349 6.2e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 632
AasI GACNNNNNNGTC 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 632
AccI GTMKAC 1 cut(s) 317
AciI CCGC 3 cut(s) 393, 678, 986
AclWI GGATC 5 cut(s) 3, 85, 513, 731, 744
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 1 cut(s) 848
AcuI CTGAAG 1 cut(s) 999
AfiI CCNNNNNNNGG 3 cut(s) 67, 264, 620
AgeI ACCGGT 1 cut(s) 60
AgsI TTSAA 4 cut(s) 98, 210, 326, 611
AjnI CCWGG 1 cut(s) 430
AluBI AGCT 6 cut(s) 383, 834, 970, 1013, 1019, 1042
AluI AGCT 6 cut(s) 383, 834, 970, 1013, 1019, 1042
Alw21I GWGCWC 2 cut(s) 296, 518
Alw26I GTCTC 2 cut(s) 668, 902
Alw44I GTGCAC 1 cut(s) 292
AlwI GGATC 5 cut(s) 3, 85, 513, 731, 744
AoxI GGCC 1 cut(s) 31
ApaLI GTGCAC 1 cut(s) 292
ApeKI GCWGC 3 cut(s) 162, 635, 638
ApoI RAATTY 1 cut(s) 848
AsiGI ACCGGT 1 cut(s) 60
AspA2I CCTAGG 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 656, 758
AsuNHI GCTAGC 2 cut(s) 379, 1019
AvaII GGWCC 1 cut(s) 58
AvrII CCTAGG 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 296
BaeI ACNNNNGTAYC 8 cut(s) 535, 535, 568, 568, 639, 639, 672, 672
BamHI GGATCC 1 cut(s) 736
BbsI GAAGAC 1 cut(s) 856
Bbv12I GWGCWC 2 cut(s) 296, 518
BbvI GCAGC 3 cut(s) 174, 622, 625
BccI CCATC 3 cut(s) 236, 876, 889
BciT130I CCWGG 1 cut(s) 432
BciVI GTATCC 1 cut(s) 667
BcoDI GTCTC 2 cut(s) 668, 902
BfaI CTAG 6 cut(s) 129, 366, 380, 755, 971, 1020
BfmI CTRYAG 1 cut(s) 639
BfuAI ACCTGC 1 cut(s) 632
BfuI GTATCC 1 cut(s) 667
BisI GCNGC 4 cut(s) 163, 636, 639, 987
BlnI CCTAGG 1 cut(s) 128
BlsI GCNGC 4 cut(s) 164, 637, 640, 988
Bme1390I CCNGG 1 cut(s) 432
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 58
BmiI GGNNCC 2 cut(s) 738, 799
BmrFI CCNGG 1 cut(s) 432
BmrI ACTGGG 1 cut(s) 710
BmsI GCATC 3 cut(s) 109, 618, 998
BmtI GCTAGC 2 cut(s) 383, 1023
BmuI ACTGGG 1 cut(s) 710
BoxI GACNNNNGTC 1 cut(s) 860
BpiI GAAGAC 1 cut(s) 856
BpmI CTGGAG 2 cut(s) 568, 784
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 3 cut(s) 128, 303, 582
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 264, 620
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 5 cut(s) 265, 551, 705, 801, 1006
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 3 cut(s) 128, 303, 582
BseGI GGATG 5 cut(s) 76, 355, 424, 633, 868
BseLI CCNNNNNNNGG 3 cut(s) 67, 264, 620
BseMII CTCAG 4 cut(s) 35, 249, 350, 611
BseNI ACTGG 5 cut(s) 265, 551, 705, 801, 1006
BseRI GAGGAG 1 cut(s) 15
BseSI GKGCMC 1 cut(s) 296
BseX3I CGGCCG 1 cut(s) 31
BseXI GCAGC 3 cut(s) 174, 622, 625
BseYI CCCAGC 1 cut(s) 732
BsgI GTGCAG 1 cut(s) 942
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 1 cut(s) 33
BshTI ACCGGT 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 2 cut(s) 296, 518
BsiSI CCGG 1 cut(s) 61
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 3 cut(s) 67, 264, 620
BsmAI GTCTC 2 cut(s) 668, 902
BsmFI GGGAC 1 cut(s) 471
BsmI GAATGC 2 cut(s) 331, 821
BsnI GGCC 1 cut(s) 33
Bso31I GGTCTC 1 cut(s) 668
Bsp1286I GDGCHC 2 cut(s) 296, 518
Bsp143I GATC 5 cut(s) 8, 77, 271, 505, 736
BspACI CCGC 3 cut(s) 393, 678, 986
BspANI GGCC 1 cut(s) 33
BspCNI CTCAG 4 cut(s) 36, 248, 349, 610
BspLI GGNNCC 2 cut(s) 738, 799
BspMAI CTGCAG 1 cut(s) 643
BspMI ACCTGC 1 cut(s) 632
BspOI GCTAGC 2 cut(s) 383, 1023
BspPI GGATC 5 cut(s) 3, 85, 513, 731, 744
BspQI GCTCTTC 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 668
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 5 cut(s) 265, 551, 705, 801, 1006
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 3 cut(s) 128, 303, 582
BssMI GATC 5 cut(s) 8, 77, 271, 505, 736
BssT1I CCWWGG 3 cut(s) 128, 303, 582
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 3 cut(s) 406, 472, 533
Bst6I CTCTTC 2 cut(s) 21, 321
BstC8I GCNNGC 5 cut(s) 381, 633, 689, 957, 1021
BstDEI CTNAG 4 cut(s) 44, 235, 336, 597
BstF5I GGATG 5 cut(s) 76, 355, 424, 633, 868
BstKTI GATC 5 cut(s) 11, 80, 274, 508, 739
BstMAI GTCTC 2 cut(s) 668, 902
BstMBI GATC 5 cut(s) 8, 77, 271, 505, 736
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 2 cut(s) 328, 804
BstNI CCWGG 1 cut(s) 432
BstNSI RCATGY 3 cut(s) 684, 861, 1009
BstPAI GACNNNNGTC 1 cut(s) 860
BstSCI CCNGG 1 cut(s) 430
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 296
BstV1I GCAGC 3 cut(s) 174, 622, 625
BstV2I GAAGAC 1 cut(s) 856
BstX2I RGATCY 3 cut(s) 77, 505, 736
BstYI RGATCY 3 cut(s) 77, 505, 736
BstZI CGGCCG 1 cut(s) 31
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 1 cut(s) 33
BtsCI GGATG 5 cut(s) 76, 355, 424, 633, 868
BtsIMutI CAGTG 3 cut(s) 258, 344, 538
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 5 cut(s) 381, 633, 689, 957, 1021
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 1 cut(s) 58
CseI GACGC 1 cut(s) 850
CspAI ACCGGT 1 cut(s) 60
CviAII CATG 8 cut(s) 269, 681, 858, 928, 962, 993, 1006, 1025
DdeI CTNAG 4 cut(s) 44, 235, 336, 597
DpnI GATC 5 cut(s) 10, 79, 273, 507, 738
DpnII GATC 5 cut(s) 8, 77, 271, 505, 736
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
Eam1104I CTCTTC 2 cut(s) 21, 321
EarI CTCTTC 2 cut(s) 21, 321
EclXI CGGCCG 1 cut(s) 31
Eco130I CCWWGG 3 cut(s) 128, 303, 582
Eco31I GGTCTC 1 cut(s) 668
Eco47I GGWCC 1 cut(s) 58
Eco52I CGGCCG 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 999
EcoRII CCWGG 1 cut(s) 430
EcoT14I CCWWGG 3 cut(s) 128, 303, 582
ErhI CCWWGG 3 cut(s) 128, 303, 582
FaeI CATG 8 cut(s) 272, 684, 861, 931, 965, 996, 1009, 1028
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 1 cut(s) 471
FatI CATG 8 cut(s) 268, 680, 857, 927, 961, 992, 1005, 1024
FblI GTMKAC 1 cut(s) 317
Fnu4HI GCNGC 4 cut(s) 163, 636, 639, 987
FokI GGATG 5 cut(s) 83, 362, 431, 640, 855
Fsp4HI GCNGC 4 cut(s) 163, 636, 639, 987
FspBI CTAG 6 cut(s) 129, 366, 380, 755, 971, 1020
GluI GCNGC 4 cut(s) 163, 636, 639, 987
GsaI CCCAGC 1 cut(s) 736
GsuI CTGGAG 2 cut(s) 568, 784
HaeIII GGCC 1 cut(s) 33
HapII CCGG 1 cut(s) 61
HgaI GACGC 1 cut(s) 850
Hin1II CATG 8 cut(s) 272, 684, 861, 931, 965, 996, 1009, 1028
HinfI GANTC 4 cut(s) 98, 299, 357, 773
HpaII CCGG 1 cut(s) 61
HphI GGTGA 2 cut(s) 656, 758
Hpy166II GTNNAC 2 cut(s) 294, 318
Hpy188I TCNGA 7 cut(s) 8, 45, 154, 184, 600, 841, 855
Hpy188III TCNNGA 1 cut(s) 556
Hpy8I GTNNAC 2 cut(s) 294, 318
HpyAV CCTTC 2 cut(s) 618, 750
HpyCH4III ACNGT 3 cut(s) 406, 472, 533
HpyCH4V TGCA 6 cut(s) 203, 294, 593, 641, 687, 959
HpyF10VI GCNNNNNNNGC 2 cut(s) 328, 804
HpyF3I CTNAG 4 cut(s) 44, 235, 336, 597
Hsp92II CATG 8 cut(s) 272, 684, 861, 931, 965, 996, 1009, 1028
Kzo9I GATC 5 cut(s) 8, 77, 271, 505, 736
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 2 cut(s) 9, 803
Lsp1109I GCAGC 3 cut(s) 174, 622, 625
LweI GCATC 3 cut(s) 109, 618, 998
MaeI CTAG 6 cut(s) 129, 366, 380, 755, 971, 1020
MaeIII GTNAC 2 cut(s) 227, 477
MalI GATC 5 cut(s) 10, 79, 273, 507, 738
MboI GATC 5 cut(s) 8, 77, 271, 505, 736
MboII GAAGA 6 cut(s) 8, 338, 381, 500, 856, 969
MflI RGATCY 3 cut(s) 77, 505, 736
MhlI GDGCHC 2 cut(s) 296, 518
MluCI AATT 5 cut(s) 91, 663, 695, 721, 848
MlyI GAGTC 2 cut(s) 308, 767
MnlI CCTC 9 cut(s) 24, 36, 39, 51, 79, 161, 496, 597, 624
MseI TTAA 2 cut(s) 693, 830
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 1 cut(s) 61
MspR9I CCNGG 1 cut(s) 432
Mva1269I GAATGC 2 cut(s) 331, 821
MvaI CCWGG 1 cut(s) 432
MwoI GCNNNNNNNGC 2 cut(s) 328, 804
NdeII GATC 5 cut(s) 8, 77, 271, 505, 736
NheI GCTAGC 2 cut(s) 379, 1019
NlaIII CATG 8 cut(s) 272, 684, 861, 931, 965, 996, 1009, 1028
NlaIV GGNNCC 2 cut(s) 738, 799
NmeAIII GCCGAG 3 cut(s) 9, 59, 124
NmuCI GTSAC 2 cut(s) 227, 477
NspI RCATGY 3 cut(s) 684, 861, 1009
PaqCI CACCTGC 1 cut(s) 632
PciSI GCTCTTC 1 cut(s) 321
PctI GAATGC 2 cut(s) 331, 821
PfeI GAWTC 2 cut(s) 98, 357
PinAI ACCGGT 1 cut(s) 60
PkrI GCNGC 4 cut(s) 164, 637, 640, 988
PleI GAGTC 2 cut(s) 307, 767
PpsI GAGTC 2 cut(s) 307, 767
PshAI GACNNNNGTC 1 cut(s) 860
Psp6I CCWGG 1 cut(s) 430
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 2 cut(s) 738, 799
PspPI GGNCC 1 cut(s) 58
PstI CTGCAG 1 cut(s) 643
PsuI RGATCY 3 cut(s) 77, 505, 736
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 2 cut(s) 693, 830
SatI GCNGC 4 cut(s) 163, 636, 639, 987
Sau3AI GATC 5 cut(s) 8, 77, 271, 505, 736
Sau96I GGNCC 1 cut(s) 58
SchI GAGTC 2 cut(s) 308, 767
ScrFI CCNGG 1 cut(s) 432
SduI GDGCHC 2 cut(s) 296, 518
SfaNI GCATC 3 cut(s) 109, 618, 998
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 1 cut(s) 58
Sse9I AATT 5 cut(s) 91, 663, 695, 721, 848
SsiI CCGC 3 cut(s) 393, 678, 986
SspMI CTAG 6 cut(s) 129, 366, 380, 755, 971, 1020
StyD4I CCNGG 1 cut(s) 430
StyI CCWWGG 3 cut(s) 128, 303, 582
TaaI ACNGT 3 cut(s) 406, 472, 533
TaqI TCGA 3 cut(s) 219, 274, 355
TasI AATT 5 cut(s) 91, 663, 695, 721, 848
TauI GCSGC 1 cut(s) 989
TfiI GAWTC 2 cut(s) 98, 357
Tru1I TTAA 2 cut(s) 693, 830
Tru9I TTAA 2 cut(s) 693, 830
TscAI CASTG 3 cut(s) 265, 344, 538
TseFI GTSAC 2 cut(s) 227, 477
TseI GCWGC 3 cut(s) 162, 635, 638
Tsp45I GTSAC 2 cut(s) 227, 477
TspDTI ATGAA 1 cut(s) 434
TspGWI ACGGA 2 cut(s) 90, 362
TspRI CASTG 3 cut(s) 265, 344, 538
VneI GTGCAC 1 cut(s) 292
VpaK11BI GGWCC 1 cut(s) 58
XapI RAATTY 1 cut(s) 848
XceI RCATGY 3 cut(s) 684, 861, 1009
XmaJI CCTAGG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 317
XspI CTAG 6 cut(s) 129, 366, 380, 755, 971, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.