Rmu_sc0000820.1_g000045

Protein BPS1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000820.1
OG0002831
Physical Location & Seq
Forward (+)
187992 .. 189245
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1254 bp
atgcatgaaagagtacttgaaatgttctatgctatccagccagtagttcgggaaaagccatctctcaaatgcattagtacttgtttaaatctactgattgaagcgaatcaggttgatttggcccaacagtttctcatgcatttgaagaagagtcttaacttgaagctgaatacatgcattgtcaacatcttggttaagcaccattgcacaaatggggatcttgaatctgccttcgaggttgtgaaggaaatgaaaaagtctaagctttcctatcctaatttgattacttactcgactctaattgatggtttatgtcaaagtggaagactgacagaagcaatggacctgtttgaggaaatgatctcaaaggatcagatattgcctgatgtgctaacgtacaacatgttgattaatcgtttttgccgtggttggaaagttaatagggcaaagatgatattggattttttgaagagcaaaggatgcaacccaaatgtattcaattactcagctctgatgaatggattctgtaaggaggaacgtaatgacataaaaactctcatagctgagattgatcttcccgtgagtgcttgggatgagaagtggattgatgtgtacttggacatcagtgtaaagttgctcgatatatgcattgtcttcagctctgagatcgcacacttaaaccaggggcatttgtttcttcagtttgtgttgcataatttgaattcagcctcttcgaagcaatttattcgtgcccgttcttcaattgatgactggaagaatcatattgcttcaaagaaccccagggttgagaactgtagcaccattttggacaagcttgtggaatcactggatctaccaaaggttaagaactcagcaaaagggaaacttttaatgtgtgctatgtatggagtgaaggtgttgacagtatctgtttgtagtgtctttatcgcagccttttctggttctgcaagcaagttgttagatttgaatgttgccgagacatatatgtgggcacaagcatttaatgacttacagagtaatgtaaatggggaaattagaaatgtactttctagtggtagagttacggtactgaaagagctggaagcagttgatggtattgtgaagcacttgtatcctatgatccaagatgacgtggcccttgcaaaagaagaagcattcaagaattctacttcagatttagaaaggaatgcacagaatctgatgggaaatgtagaagggcaggttgtgaggtga

Protein Analysis

417

Amino Acids

47.08

Weight (kDa)

6.76

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 51 - 102 9.3e-07 Pentatricopeptide repeat domain
PPR_long PF17177 51 - 123 6.4e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 53 - 85 7.7e-06 PPR repeat
PPR_2 PF13041 57 - 106 2.4e-13 PPR repeat family
PPR_2 PF13041 92 - 142 1.6e-15 PPR repeat family
PPR_1 PF12854 92 - 120 2.8e-13 PPR repeat
PPR_3 PF13812 92 - 140 2.6e-06 Pentatricopeptide repeat domain
PPR PF01535 95 - 123 1.6e-09 PPR repeat
PPR_3 PF13812 117 - 175 5.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 126 - 154 3.8e-06 PPR repeat
PPR_2 PF13041 128 - 177 5.4e-13 PPR repeat family
PPR_1 PF12854 159 - 182 8.3e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1231
AclWI GGATC 4 cut(s) 225, 378, 858, 1135
AcsI RAATTY 2 cut(s) 721, 1183
AcuI CTGAAG 3 cut(s) 640, 683, 1176
AfaI GTAC 6 cut(s) 15, 79, 398, 614, 1065, 1089
AfiI CCNNNNNNNGG 1 cut(s) 352
AflIII ACRYGT 1 cut(s) 402
AjiI CACGTC 1 cut(s) 1153
AjnI CCWGG 2 cut(s) 681, 800
AluBI AGCT 7 cut(s) 166, 265, 509, 563, 660, 835, 1099
AluI AGCT 7 cut(s) 166, 265, 509, 563, 660, 835, 1099
Alw26I GTCTC 1 cut(s) 992
AlwI GGATC 4 cut(s) 225, 378, 858, 1135
AlwNI CAGNNNCTG 2 cut(s) 929, 1219
AoxI GGCC 2 cut(s) 120, 1155
ApeKI GCWGC 1 cut(s) 950
ApoI RAATTY 2 cut(s) 721, 1183
AseI ATTAAT 1 cut(s) 411
Asp700I GAANNNNTTC 1 cut(s) 521
AspS9I GGNCC 3 cut(s) 121, 343, 1156
AsuII TTCGAA 1 cut(s) 734
AvaII GGWCC 1 cut(s) 343
BaeGI GKGCMC 2 cut(s) 754, 1015
BbsI GAAGAC 2 cut(s) 331, 646
BbvI GCAGC 1 cut(s) 962
BccI CCATC 4 cut(s) 67, 299, 1106, 1216
BceAI ACGGC 1 cut(s) 408
BcgI CGANNNNNNTGC 2 cut(s) 728, 762
BciT130I CCWGG 2 cut(s) 683, 802
BciVI GTATCC 1 cut(s) 1143
BcoDI GTCTC 1 cut(s) 992
BfaI CTAG 1 cut(s) 1071
BfmI CTRYAG 1 cut(s) 814
BfuAI ACCTGC 1 cut(s) 1231
BfuI GTATCC 1 cut(s) 1143
BisI GCNGC 1 cut(s) 951
BlsI GCNGC 1 cut(s) 952
BmcAI AGTACT 2 cut(s) 15, 79
Bme1390I CCNGG 2 cut(s) 683, 802
Bme18I GGWCC 1 cut(s) 343
BmgBI CACGTC 1 cut(s) 1153
BmgT120I GGNCC 3 cut(s) 121, 343, 1156
BmrFI CCNGG 2 cut(s) 683, 802
BmsI GCATC 1 cut(s) 470
BpiI GAAGAC 2 cut(s) 331, 646
Bpu14I TTCGAA 1 cut(s) 734
BsaJI CCNNGG 4 cut(s) 424, 682, 800, 801
BsaXI ACNNNNNCTCC 2 cut(s) 900, 930
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse1I ACTGG 3 cut(s) 41, 776, 852
Bse3DI GCAATG 2 cut(s) 202, 345
BseBI CCWGG 2 cut(s) 683, 802
BseDI CCNNGG 4 cut(s) 424, 682, 800, 801
BseGI GGATG 2 cut(s) 485, 598
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 2 cut(s) 202, 345
BseMII CTCAG 4 cut(s) 519, 555, 654, 885
BseNI ACTGG 3 cut(s) 41, 776, 852
BseSI GKGCMC 2 cut(s) 754, 1015
BseXI GCAGC 1 cut(s) 962
BshFI GGCC 2 cut(s) 122, 1157
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 1 cut(s) 992
BsmI GAATGC 2 cut(s) 1175, 1213
BsnI GGCC 2 cut(s) 122, 1157
Bsp119I TTCGAA 1 cut(s) 734
Bsp1286I GDGCHC 2 cut(s) 754, 1015
Bsp143I GATC 7 cut(s) 217, 360, 370, 571, 666, 850, 1140
BspANI GGCC 2 cut(s) 122, 1157
BspCNI CTCAG 4 cut(s) 518, 556, 655, 884
BspMI ACCTGC 1 cut(s) 1231
BspPI GGATC 4 cut(s) 225, 378, 858, 1135
BspQI GCTCTTC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 734
BsrDI GCAATG 2 cut(s) 202, 345
BsrI ACTGG 3 cut(s) 41, 776, 852
BssECI CCNNGG 4 cut(s) 424, 682, 800, 801
BssMI GATC 7 cut(s) 217, 360, 370, 571, 666, 850, 1140
Bst2UI CCWGG 2 cut(s) 683, 802
Bst4CI ACNGT 4 cut(s) 129, 815, 925, 1087
Bst6I CTCTTC 3 cut(s) 143, 464, 736
BstAPI GCANNNNNTGC 1 cut(s) 480
BstBI TTCGAA 1 cut(s) 734
BstC8I GCNNGC 1 cut(s) 970
BstDEI CTNAG 5 cut(s) 261, 505, 564, 663, 871
BstDSI CCRYGG 1 cut(s) 424
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 485, 598
BstKTI GATC 7 cut(s) 220, 363, 373, 574, 669, 853, 1143
BstMAI GTCTC 1 cut(s) 992
BstMBI GATC 7 cut(s) 217, 360, 370, 571, 666, 850, 1140
BstMWI GCNNNNNNNGC 2 cut(s) 388, 480
BstNI CCWGG 2 cut(s) 683, 802
BstNSI RCATGY 2 cut(s) 177, 406
BstSCI CCNGG 2 cut(s) 681, 800
BstSFI CTRYAG 1 cut(s) 814
BstSLI GKGCMC 2 cut(s) 754, 1015
BstV1I GCAGC 1 cut(s) 962
BstV2I GAAGAC 2 cut(s) 331, 646
BstX2I RGATCY 2 cut(s) 217, 850
BstYI RGATCY 2 cut(s) 217, 850
BsuI GTATCC 1 cut(s) 1143
BsuRI GGCC 2 cut(s) 122, 1157
BtgI CCRYGG 1 cut(s) 424
BtrI CACGTC 1 cut(s) 1153
BtsCI GGATG 2 cut(s) 485, 598
BtsIMutI CAGTG 2 cut(s) 631, 845
BveI ACCTGC 1 cut(s) 1231
Cac8I GCNNGC 1 cut(s) 970
CaiI CAGNNNCTG 2 cut(s) 929, 1219
Cfr13I GGNCC 3 cut(s) 121, 343, 1156
Csp6I GTAC 6 cut(s) 14, 78, 397, 613, 1064, 1088
CviAII CATG 4 cut(s) 5, 136, 174, 403
CviQI GTAC 6 cut(s) 14, 78, 397, 613, 1064, 1088
DdeI CTNAG 5 cut(s) 261, 505, 564, 663, 871
DpnI GATC 7 cut(s) 219, 362, 372, 573, 668, 852, 1142
DpnII GATC 7 cut(s) 217, 360, 370, 571, 666, 850, 1140
DraI TTTAAA 1 cut(s) 87
Eam1104I CTCTTC 3 cut(s) 143, 464, 736
EarI CTCTTC 3 cut(s) 143, 464, 736
Eco47I GGWCC 1 cut(s) 343
Eco57I CTGAAG 3 cut(s) 640, 683, 1176
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoRI GAATTC 2 cut(s) 721, 1183
EcoRII CCWGG 2 cut(s) 681, 800
EcoT22I ATGCAT 5 cut(s) 6, 74, 141, 179, 650
FaeI CATG 4 cut(s) 8, 139, 177, 406
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FatI CATG 4 cut(s) 4, 135, 173, 402
Fnu4HI GCNGC 1 cut(s) 951
FokI GGATG 2 cut(s) 492, 605
Fsp4HI GCNGC 1 cut(s) 951
FspBI CTAG 1 cut(s) 1071
GluI GCNGC 1 cut(s) 951
HaeIII GGCC 2 cut(s) 122, 1157
Hin1II CATG 4 cut(s) 8, 139, 177, 406
HincII GTYRAC 2 cut(s) 184, 921
HindII GTYRAC 2 cut(s) 184, 921
HindIII AAGCTT 2 cut(s) 263, 833
HinfI GANTC 8 cut(s) 106, 151, 224, 295, 522, 778, 842, 1216
Hpy166II GTNNAC 3 cut(s) 184, 613, 921
Hpy188I TCNGA 5 cut(s) 375, 513, 664, 1195, 1221
Hpy188III TCNNGA 3 cut(s) 50, 221, 1180
Hpy8I GTNNAC 3 cut(s) 184, 613, 921
HpyAV CCTTC 4 cut(s) 238, 241, 907, 1229
HpyCH4III ACNGT 4 cut(s) 129, 815, 925, 1087
HpyCH4IV ACGT 3 cut(s) 395, 538, 1152
HpyF10VI GCNNNNNNNGC 2 cut(s) 388, 480
HpyF3I CTNAG 5 cut(s) 261, 505, 564, 663, 871
HpySE526I ACGT 3 cut(s) 395, 538, 1152
Hsp92II CATG 4 cut(s) 8, 139, 177, 406
Kzo9I GATC 7 cut(s) 217, 360, 370, 571, 666, 850, 1140
LguI GCTCTTC 1 cut(s) 464
Lsp1109I GCAGC 1 cut(s) 962
LweI GCATC 1 cut(s) 470
MaeI CTAG 1 cut(s) 1071
MaeII ACGT 3 cut(s) 395, 538, 1152
MaeIII GTNAC 1 cut(s) 1081
MalI GATC 7 cut(s) 219, 362, 372, 573, 668, 852, 1142
MboI GATC 7 cut(s) 217, 360, 370, 571, 666, 850, 1140
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 2 cut(s) 217, 850
MhlI GDGCHC 2 cut(s) 754, 1015
MluCI AATT 9 cut(s) 277, 300, 499, 715, 721, 740, 762, 1053, 1183
MlyI GAGTC 2 cut(s) 160, 289
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 5 cut(s) 229, 346, 526, 739, 1242
Mph1103I ATGCAT 5 cut(s) 6, 74, 141, 179, 650
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 9 cut(s) 86, 156, 195, 411, 438, 677, 864, 890, 1023
MslI CAYNNNNRTG 1 cut(s) 1006
MspR9I CCNGG 2 cut(s) 683, 802
MunI CAATTG 1 cut(s) 762
Mva1269I GAATGC 2 cut(s) 1175, 1213
MvaI CCWGG 2 cut(s) 683, 802
MwoI GCNNNNNNNGC 2 cut(s) 388, 480
NdeII GATC 7 cut(s) 217, 360, 370, 571, 666, 850, 1140
NlaIII CATG 4 cut(s) 8, 139, 177, 406
NmeAIII GCCGAG 1 cut(s) 1021
NsiI ATGCAT 5 cut(s) 6, 74, 141, 179, 650
NspI RCATGY 2 cut(s) 177, 406
NspV TTCGAA 1 cut(s) 734
PasI CCCWGGG 1 cut(s) 801
PciI ACATGT 1 cut(s) 402
PciSI GCTCTTC 1 cut(s) 464
PcsI WCGNNNNNNNCGW 1 cut(s) 421
PctI GAATGC 2 cut(s) 1175, 1213
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 6 cut(s) 106, 224, 522, 778, 842, 1216
PkrI GCNGC 1 cut(s) 952
PleI GAGTC 2 cut(s) 159, 289
PpsI GAGTC 2 cut(s) 159, 289
PscI ACATGT 1 cut(s) 402
PshBI ATTAAT 1 cut(s) 411
Psp6I CCWGG 2 cut(s) 681, 800
PspGI CCWGG 2 cut(s) 681, 800
PspPI GGNCC 3 cut(s) 121, 343, 1156
PstNI CAGNNNCTG 2 cut(s) 929, 1219
PsuI RGATCY 2 cut(s) 217, 850
RsaI GTAC 6 cut(s) 15, 79, 398, 614, 1065, 1089
RsaNI GTAC 6 cut(s) 14, 78, 397, 613, 1064, 1088
RseI CAYNNNNRTG 1 cut(s) 1006
SapI GCTCTTC 1 cut(s) 464
SaqAI TTAA 9 cut(s) 86, 156, 195, 411, 438, 677, 864, 890, 1023
SatI GCNGC 1 cut(s) 951
Sau3AI GATC 7 cut(s) 217, 360, 370, 571, 666, 850, 1140
Sau96I GGNCC 3 cut(s) 121, 343, 1156
ScaI AGTACT 2 cut(s) 15, 79
SchI GAGTC 2 cut(s) 160, 289
ScrFI CCNGG 2 cut(s) 683, 802
SduI GDGCHC 2 cut(s) 754, 1015
SfaNI GCATC 1 cut(s) 470
SfcI CTRYAG 1 cut(s) 814
SfuI TTCGAA 1 cut(s) 734
SinI GGWCC 1 cut(s) 343
SmiMI CAYNNNNRTG 1 cut(s) 1006
Sse9I AATT 9 cut(s) 277, 300, 499, 715, 721, 740, 762, 1053, 1183
SspMI CTAG 1 cut(s) 1071
StyD4I CCNGG 2 cut(s) 681, 800
TaaI ACNGT 4 cut(s) 129, 815, 925, 1087
TaiI ACGT 3 cut(s) 398, 541, 1155
TaqI TCGA 4 cut(s) 234, 293, 639, 734
TasI AATT 9 cut(s) 277, 300, 499, 715, 721, 740, 762, 1053, 1183
TatI WGTACW 4 cut(s) 13, 77, 612, 1063
TfiI GAWTC 6 cut(s) 106, 224, 522, 778, 842, 1216
Tru1I TTAA 9 cut(s) 86, 156, 195, 411, 438, 677, 864, 890, 1023
Tru9I TTAA 9 cut(s) 86, 156, 195, 411, 438, 677, 864, 890, 1023
TscAI CASTG 2 cut(s) 631, 852
TseI GCWGC 1 cut(s) 950
TspDTI ATGAA 3 cut(s) 21, 266, 530
TspRI CASTG 2 cut(s) 631, 852
VpaK11BI GGWCC 1 cut(s) 343
VspI ATTAAT 1 cut(s) 411
XagI CCTNNNNNAGG 1 cut(s) 350
XapI RAATTY 2 cut(s) 721, 1183
XceI RCATGY 2 cut(s) 177, 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 209
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 1 cut(s) 1071
ZrmI AGTACT 2 cut(s) 15, 79
Zsp2I ATGCAT 5 cut(s) 6, 74, 141, 179, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.