Rmu_sc0000838.1_g000011

peptidase inhibitor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000838.1
OG0008544
Physical Location & Seq
Reverse (-)
62537 .. 65751
3215 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
atggccaagcaaaccagcgaagaaaccagcagcaccaccatagagaaaatagccatagccggcggcttagtctccaacccggtgattggttggtccctctacactctcaagaccaccgggtgtggcctgcctcccggacccggtggatccatcggtgcacttgaaggtgtcagctacctagctgtgctgggaatagtgggttggtctttgtacaccaagaccaaaacaggctcgggtctgcccaatggaccatttggtttgttcagcagggctccattccaggacccctcccaactgatcagtgctttggatgaagatgaagatgatgataatcagactagcagacaaggtgacgatggcaataagctattgtggctggacagggtgaccggaagggtttgcacgtcgatttctgcaaaggccatggctataacagggattgaggatcggcgatactggaactggattgctactgaagaatcaaggagtcaaaaagtggaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

17.84

Weight (kDa)

4.81

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 133 - 166 1.1e-06 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 63
AclWI GGATC 3 cut(s) 141, 154, 453
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 495
AdeI CACNNNGTG 1 cut(s) 120
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 2 cut(s) 86, 140
AgsI TTSAA 1 cut(s) 164
AjiI CACGTC 1 cut(s) 405
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 184, 216
AluBI AGCT 3 cut(s) 174, 182, 367
AluI AGCT 3 cut(s) 174, 182, 367
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 1 cut(s) 76
Alw44I GTGCAC 1 cut(s) 156
AlwI GGATC 3 cut(s) 141, 154, 453
Ama87I CYCGRG 1 cut(s) 232
AoxI GGCC 3 cut(s) 3, 124, 420
ApaLI GTGCAC 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 30
AspS9I GGNCC 4 cut(s) 93, 137, 248, 283
AsuC2I CCSGG 4 cut(s) 80, 118, 135, 141
AsuHPI GGTGA 3 cut(s) 94, 362, 397
AvaI CYCGRG 1 cut(s) 232
AvaII GGWCC 4 cut(s) 93, 137, 248, 283
BaeGI GKGCMC 1 cut(s) 160
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 1 cut(s) 42
BccI CCATC 2 cut(s) 158, 350
BciT130I CCWGG 1 cut(s) 281
BclI TGATCA 1 cut(s) 297
BcnI CCSGG 4 cut(s) 80, 118, 135, 141
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 2 cut(s) 179, 339
BisI GCNGC 2 cut(s) 31, 64
BlsI GCNGC 2 cut(s) 32, 65
Bme1390I CCNGG 5 cut(s) 80, 118, 135, 141, 281
Bme18I GGWCC 4 cut(s) 93, 137, 248, 283
BmeT110I CYCGRG 1 cut(s) 232
BmgBI CACGTC 1 cut(s) 405
BmgT120I GGNCC 4 cut(s) 93, 137, 248, 283
BmiI GGNNCC 5 cut(s) 95, 139, 148, 273, 285
BmrFI CCNGG 5 cut(s) 80, 118, 135, 141, 281
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 4 cut(s) 80, 118, 135, 141
BsaBI GATNNNNATC 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 423
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 140
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 2 cut(s) 461, 467
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 1 cut(s) 316
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 2 cut(s) 86, 140
BseNI ACTGG 2 cut(s) 461, 467
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 1 cut(s) 42
BseYI CCCAGC 1 cut(s) 187
BshFI GGCC 3 cut(s) 5, 126, 422
BsiHKAI GWGCWC 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 6 cut(s) 60, 80, 117, 135, 141, 390
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 86, 140
BsmAI GTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 3 cut(s) 5, 126, 422
BsoBI CYCGRG 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 160, 274
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 3 cut(s) 146, 297, 445
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 1 cut(s) 63
BspANI GGCC 3 cut(s) 5, 126, 422
BspLI GGNNCC 5 cut(s) 95, 139, 148, 273, 285
BspPI GGATC 3 cut(s) 141, 154, 453
BsrFI RCCGGY 1 cut(s) 59
BsrGI TGTACA 1 cut(s) 210
BsrI ACTGG 2 cut(s) 461, 467
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 3 cut(s) 146, 297, 445
BssT1I CCWWGG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 281
BstAUI TGTACA 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 61, 128
BstDEI CTNAG 1 cut(s) 67
BstDSI CCRYGG 1 cut(s) 423
BstEII GGTNACC 1 cut(s) 385
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 3 cut(s) 149, 300, 448
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 3 cut(s) 146, 297, 445
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstNI CCWGG 1 cut(s) 281
BstPI GGTNACC 1 cut(s) 385
BstSCI CCNGG 5 cut(s) 78, 116, 133, 139, 279
BstSLI GKGCMC 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 3 cut(s) 5, 126, 422
BtgI CCRYGG 1 cut(s) 423
BtrI CACGTC 1 cut(s) 405
BtsCI GGATG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 307
Cac8I GCNNGC 2 cut(s) 61, 128
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 4 cut(s) 93, 137, 248, 283
Csp6I GTAC 1 cut(s) 211
CviAII CATG 1 cut(s) 424
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 1 cut(s) 67
DpnI GATC 3 cut(s) 148, 299, 447
DpnII GATC 3 cut(s) 146, 297, 445
DraIII CACNNNGTG 1 cut(s) 120
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 274
Eco47I GGWCC 4 cut(s) 93, 137, 248, 283
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 232
Eco91I GGTNACC 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 283
EcoO65I GGTNACC 1 cut(s) 385
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 1 cut(s) 427
FaiI YATR 4 cut(s) 41, 56, 425, 431
FaqI GGGAC 1 cut(s) 79
FatI CATG 1 cut(s) 423
FbaI TGATCA 1 cut(s) 297
Fnu4HI GCNGC 2 cut(s) 31, 64
FokI GGATG 1 cut(s) 323
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 2 cut(s) 31, 64
FspBI CTAG 2 cut(s) 179, 339
GluI GCNGC 2 cut(s) 31, 64
GsaI CCCAGC 1 cut(s) 191
HaeIII GGCC 3 cut(s) 5, 126, 422
HapII CCGG 6 cut(s) 60, 80, 117, 135, 141, 390
Hin1II CATG 1 cut(s) 427
HinfI GANTC 2 cut(s) 479, 487
HpaII CCGG 6 cut(s) 60, 80, 117, 135, 141, 390
HphI GGTGA 3 cut(s) 94, 362, 397
Hpy166II GTNNAC 2 cut(s) 158, 213
Hpy188I TCNGA 1 cut(s) 336
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 2 cut(s) 158, 213
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 2 cut(s) 158, 387
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 3 cut(s) 158, 402, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
HpyF3I CTNAG 1 cut(s) 67
HpySE526I ACGT 1 cut(s) 404
Hsp92II CATG 1 cut(s) 427
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 3 cut(s) 146, 297, 445
LmnI GCTCC 1 cut(s) 277
Lsp1109I GCAGC 1 cut(s) 42
MaeI CTAG 2 cut(s) 179, 339
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 2 cut(s) 350, 385
MalI GATC 3 cut(s) 148, 299, 447
MboI GATC 3 cut(s) 146, 297, 445
MboII GAAGA 4 cut(s) 32, 326, 332, 488
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 2 cut(s) 160, 274
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 496
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 4 cut(s) 107, 141, 298, 436
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 59
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 6 cut(s) 60, 80, 117, 135, 141, 390
MspR9I CCNGG 5 cut(s) 80, 118, 135, 141, 281
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 1 cut(s) 373
NaeI GCCGGC 1 cut(s) 61
NciI CCSGG 4 cut(s) 80, 118, 135, 141
NcoI CCATGG 1 cut(s) 423
NdeII GATC 3 cut(s) 146, 297, 445
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 1 cut(s) 427
NlaIV GGNNCC 5 cut(s) 95, 139, 148, 273, 285
NmuCI GTSAC 2 cut(s) 350, 385
PdiI GCCGGC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 479
PfoI TCCNGGA 2 cut(s) 133, 279
PkrI GCNGC 2 cut(s) 32, 65
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
PpuMI RGGWCCY 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 279
PspEI GGTNACC 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 5 cut(s) 95, 139, 148, 273, 285
PspPI GGNCC 4 cut(s) 93, 137, 248, 283
PspPPI RGGWCCY 1 cut(s) 283
PsuI RGATCY 1 cut(s) 146
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
SatI GCNGC 2 cut(s) 31, 64
Sau3AI GATC 3 cut(s) 146, 297, 445
Sau96I GGNCC 4 cut(s) 93, 137, 248, 283
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 5 cut(s) 80, 118, 135, 141, 281
SduI GDGCHC 2 cut(s) 160, 274
SetI ASST 7 cut(s) 169, 176, 180, 184, 352, 369, 407
SinI GGWCC 4 cut(s) 93, 137, 248, 283
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SsiI CCGC 1 cut(s) 63
SspMI CTAG 2 cut(s) 179, 339
StyD4I CCNGG 5 cut(s) 78, 116, 133, 139, 279
StyI CCWWGG 1 cut(s) 423
TaiI ACGT 1 cut(s) 407
TaqI TCGA 1 cut(s) 407
TatI WGTACW 1 cut(s) 210
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 1 cut(s) 479
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 2 cut(s) 350, 385
TseI GCWGC 1 cut(s) 30
Tsp45I GTSAC 2 cut(s) 350, 385
TspDTI ATGAA 2 cut(s) 327, 333
TspRI CASTG 1 cut(s) 307
VneI GTGCAC 1 cut(s) 156
VpaK11BI GGWCC 4 cut(s) 93, 137, 248, 283
XspI CTAG 2 cut(s) 179, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.