Rmu_sc0001354.1_g000021

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001354.1
OG0005281
Physical Location & Seq
Forward (+)
79241 .. 81485
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1533 bp
atgactgggcttgtcatggtgacgaggggaggagggtgcggtggtaatttgaaaggggtcaaaggagggtcaacgaaggaggaggagcagaaccagctctctctggtggactttctcttgtctgctataaggaaatccatggtggcttgccgggttgtctcttcaacaccaacattaattcattcctacattctagagagagaaagagatgactgggcttgtaatggtgacgaggggaggagggtgcggtggaaatccatggtggcttgccgggttgagcaaggtgaggaaatgatctcggccgtccagaacttggacatcgggtggcccaccaatgtccggcacattacgcatgtcacattcgaccgcttcaatgggtttctgggacttcctgttgagtttgaggttgagattcctggtcgtgttccgagtgctagtgcaagtgtgtttggagtctcagtggattcaatgcagctgtccttcgattcaagagggaatagtgtgcctacaattctgcttctaatgcaggagaggttatatgcacatggaggtttgaaggctgaaggaatatttcgtataaacccagagaatggccaagaggagattgtgagaggccaattgaacaggggcattgtgccggatgacgttgatgttcattgtctgtctggtctgattaaggcctggtttcgagagcttccttcaggagtgctagatggactatcccccgaacaagttcttcagtgtgagacagaagaggactgcgttgagcttgtgaagcagttaaagccaactgaggctgcattgctcaattgggctattgatctgatggccgatgttgttgaggaagaagaattcaacaagatgaatgcaagaaacatcgctatggtttttgccccaaacatgactcagatgtctgatccattaacggctttgatgcatgcggttcaagtaatgaatttgcttaagaccctgatcacgaaaacactaaatgagcgtgaagagactgaaagcggatcatattcacagtccccaatgtcatcttgttcatctgagggtcaaacagatgactttgatagtcagcacgaggttgaaagcagctccgcgttaatcaagacttcaccaacatcagattatgatgaaaatggcagcagcagcaacaacagtgaagatgaatctgaagtcaagttactgaatgagatagaagaatgcttacgacaattggatgagaataaactttcaacaaatagcttggtaatgttgggtgaactaacatgcgagttgcaaggtgagtctttcagtcctcagtgctgctctaatgtggagtccgccacatcctttactgatagcaaaaatagggattctggctcgagtacaagtgacggggaagaagaagacactgcgaaatctatcatagctgtggcacaggactctgatattaaaaaccatacaagcataaatgaaatggagatggtggacagatctgcggagtctgttttgcctatgccattgactgcatctactggatttgtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

510

Amino Acids

55.92

Weight (kDa)

4.47

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 169 - 303 3.6e-19 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 910
AccB7I CCANNNNNTGG 2 cut(s) 313, 590
AccII CGCG 1 cut(s) 1103
AciI CCGC 8 cut(s) 39, 247, 367, 941, 1011, 1101, 1326, 1484
AclWI GGATC 2 cut(s) 911, 1021
AcoI YGGCCR 3 cut(s) 300, 592, 828
AcsI RAATTY 2 cut(s) 851, 955
AcuI CTGAAG 4 cut(s) 582, 684, 722, 1197
AfaI GTAC 1 cut(s) 1372
AfiI CCNNNNNNNGG 3 cut(s) 313, 339, 590
AflII CTTAAG 1 cut(s) 962
AjnI CCWGG 2 cut(s) 415, 680
AluBI AGCT 7 cut(s) 97, 475, 694, 769, 1098, 1248, 1415
AluI AGCT 7 cut(s) 97, 475, 694, 769, 1098, 1248, 1415
Alw26I GTCTC 4 cut(s) 163, 460, 740, 995
AlwI GGATC 2 cut(s) 911, 1021
Ama87I CYCGRG 1 cut(s) 1366
AoxI GGCC 6 cut(s) 300, 326, 592, 613, 678, 828
ApeKI GCWGC 7 cut(s) 472, 797, 1095, 1146, 1149, 1152, 1308
ApoI RAATTY 2 cut(s) 851, 955
AseI ATTAAT 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 732
AspS9I GGNCC 1 cut(s) 327
AsuC2I CCSGG 2 cut(s) 152, 272
AsuHPI GGTGA 6 cut(s) 31, 239, 296, 1110, 1274, 1298
AvaI CYCGRG 1 cut(s) 1366
BalI TGGCCA 1 cut(s) 594
BarI GAAGNNNNNNTAC 4 cut(s) 1170, 1194, 1202, 1226
BauI CACGAG 1 cut(s) 1082
BbsI GAAGAC 1 cut(s) 1398
BbvI GCAGC 7 cut(s) 484, 784, 1107, 1158, 1161, 1164, 1295
BccI CCATC 3 cut(s) 707, 820, 1462
BceAI ACGGC 2 cut(s) 287, 942
BciT130I CCWGG 2 cut(s) 417, 682
BclI TGATCA 1 cut(s) 972
BcnI CCSGG 2 cut(s) 152, 272
BcoDI GTCTC 4 cut(s) 163, 460, 740, 995
BfaI CTAG 3 cut(s) 194, 435, 710
BfrI CTTAAG 1 cut(s) 962
BglII AGATCT 1 cut(s) 1478
BisI GCNGC 7 cut(s) 473, 798, 1096, 1147, 1150, 1153, 1309
BlsI GCNGC 7 cut(s) 474, 799, 1097, 1148, 1151, 1154, 1310
Bme1390I CCNGG 4 cut(s) 152, 272, 417, 682
BmeT110I CYCGRG 1 cut(s) 1366
BmgT120I GGNCC 1 cut(s) 327
BmrFI CCNGG 4 cut(s) 152, 272, 417, 682
BmrI ACTGGG 2 cut(s) 15, 223
BmsI GCATC 2 cut(s) 924, 1523
BmuI ACTGGG 2 cut(s) 15, 223
BpiI GAAGAC 1 cut(s) 1398
BpuMI CCSGG 2 cut(s) 152, 272
BsaJI CCNNGG 2 cut(s) 138, 258
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 339, 590
Bse1I ACTGG 3 cut(s) 10, 218, 1525
Bse3DI GCAATG 1 cut(s) 800
BseBI CCWGG 2 cut(s) 417, 682
BseDI CCNNGG 2 cut(s) 138, 258
BseGI GGATG 3 cut(s) 646, 1228, 1331
BseLI CCNNNNNNNGG 3 cut(s) 313, 339, 590
BseMI GCAATG 1 cut(s) 800
BseMII CTCAG 5 cut(s) 471, 783, 920, 1041, 1316
BseNI ACTGG 3 cut(s) 10, 218, 1525
BseRI GAGGAG 5 cut(s) 45, 95, 98, 253, 614
BseX3I CGGCCG 1 cut(s) 300
BseXI GCAGC 7 cut(s) 484, 784, 1107, 1158, 1161, 1164, 1295
Bsh1236I CGCG 1 cut(s) 1103
Bsh1285I CGRYCG 2 cut(s) 303, 367
BshFI GGCC 6 cut(s) 302, 328, 594, 615, 680, 830
BsiEI CGRYCG 2 cut(s) 303, 367
BsiHKCI CYCGRG 1 cut(s) 1366
BsiSI CCGG 4 cut(s) 151, 271, 340, 638
BslFI GGGAC 2 cut(s) 399, 1012
BslI CCNNNNNNNGG 3 cut(s) 313, 339, 590
BsmAI GTCTC 4 cut(s) 163, 460, 740, 995
BsmFI GGGAC 2 cut(s) 399, 1012
BsmI GAATGC 2 cut(s) 871, 1211
BsnI GGCC 6 cut(s) 302, 328, 594, 615, 680, 830
BsoBI CYCGRG 1 cut(s) 1366
Bsp143I GATC 6 cut(s) 294, 820, 916, 972, 1013, 1478
Bsp19I CCATGG 2 cut(s) 138, 258
BspACI CCGC 8 cut(s) 39, 247, 367, 941, 1011, 1101, 1326, 1484
BspANI GGCC 6 cut(s) 302, 328, 594, 615, 680, 830
BspCNI CTCAG 5 cut(s) 470, 784, 919, 1042, 1315
BspFNI CGCG 1 cut(s) 1103
BspPI GGATC 2 cut(s) 911, 1021
BspTI CTTAAG 1 cut(s) 962
BsrDI GCAATG 1 cut(s) 800
BsrI ACTGG 3 cut(s) 10, 218, 1525
BssECI CCNNGG 2 cut(s) 138, 258
BssMI GATC 6 cut(s) 294, 820, 916, 972, 1013, 1478
BssSI CACGAG 1 cut(s) 1082
BssT1I CCWWGG 2 cut(s) 138, 258
Bst2BI CACGAG 1 cut(s) 1082
Bst2UI CCWGG 2 cut(s) 417, 682
Bst4CI ACNGT 2 cut(s) 1026, 1163
Bst6I CTCTTC 3 cut(s) 166, 747, 993
BstAFI CTTAAG 1 cut(s) 962
BstC8I GCNNGC 3 cut(s) 148, 268, 939
BstDEI CTNAG 5 cut(s) 457, 792, 906, 1050, 1302
BstDSI CCRYGG 2 cut(s) 138, 258
BstF5I GGATG 3 cut(s) 646, 1228, 1331
BstFNI CGCG 1 cut(s) 1103
BstKTI GATC 6 cut(s) 297, 823, 919, 975, 1016, 1481
BstMAI GTCTC 4 cut(s) 163, 460, 740, 995
BstMBI GATC 6 cut(s) 294, 820, 916, 972, 1013, 1478
BstMCI CGRYCG 2 cut(s) 303, 367
BstMWI GCNNNNNNNGC 6 cut(s) 94, 349, 523, 775, 784, 1152
BstNI CCWGG 2 cut(s) 417, 682
BstNSI RCATGY 3 cut(s) 356, 941, 1275
BstSCI CCNGG 4 cut(s) 150, 270, 415, 680
BstUI CGCG 1 cut(s) 1103
BstV1I GCAGC 7 cut(s) 484, 784, 1107, 1158, 1161, 1164, 1295
BstV2I GAAGAC 1 cut(s) 1398
BstX2I RGATCY 1 cut(s) 1478
BstYI RGATCY 1 cut(s) 1478
BstZI CGGCCG 1 cut(s) 300
BsuRI GGCC 6 cut(s) 302, 328, 594, 615, 680, 830
BtgI CCRYGG 2 cut(s) 138, 258
BtgZI GCGATG 1 cut(s) 862
BtsCI GGATG 3 cut(s) 646, 1228, 1331
BtsI GCAGTG 1 cut(s) 1395
BtsIMutI CAGTG 5 cut(s) 465, 746, 1168, 1310, 1395
Cac8I GCNNGC 3 cut(s) 148, 268, 939
Cfr13I GGNCC 1 cut(s) 327
Csp6I GTAC 1 cut(s) 1371
CviAII CATG 8 cut(s) 16, 139, 259, 353, 545, 901, 938, 1272
CviQI GTAC 1 cut(s) 1371
DdeI CTNAG 5 cut(s) 457, 792, 906, 1050, 1302
DpnI GATC 6 cut(s) 296, 822, 918, 974, 1015, 1480
DpnII GATC 6 cut(s) 294, 820, 916, 972, 1013, 1478
DrdI GACNNNNNNGTC 1 cut(s) 910
DseDI GACNNNNNNGTC 1 cut(s) 910
EaeI YGGCCR 3 cut(s) 300, 592, 828
EagI CGGCCG 1 cut(s) 300
Eam1104I CTCTTC 3 cut(s) 166, 747, 993
EarI CTCTTC 3 cut(s) 166, 747, 993
EciI GGCGGA 1 cut(s) 1315
EclXI CGGCCG 1 cut(s) 300
Eco130I CCWWGG 2 cut(s) 138, 258
Eco147I AGGCCT 1 cut(s) 680
Eco52I CGGCCG 1 cut(s) 300
Eco57I CTGAAG 4 cut(s) 582, 684, 722, 1197
Eco88I CYCGRG 1 cut(s) 1366
EcoRI GAATTC 1 cut(s) 851
EcoRII CCWGG 2 cut(s) 415, 680
EcoT14I CCWWGG 2 cut(s) 138, 258
EcoT22I ATGCAT 1 cut(s) 939
ErhI CCWWGG 2 cut(s) 138, 258
FaeI CATG 8 cut(s) 19, 142, 262, 356, 548, 904, 941, 1275
FaqI GGGAC 2 cut(s) 399, 1012
FatI CATG 8 cut(s) 15, 138, 258, 352, 544, 900, 937, 1271
FbaI TGATCA 1 cut(s) 972
Fnu4HI GCNGC 7 cut(s) 473, 798, 1096, 1147, 1150, 1153, 1309
FokI GGATG 3 cut(s) 653, 1235, 1318
Fsp4HI GCNGC 7 cut(s) 473, 798, 1096, 1147, 1150, 1153, 1309
FspBI CTAG 3 cut(s) 194, 435, 710
GluI GCNGC 7 cut(s) 473, 798, 1096, 1147, 1150, 1153, 1309
HaeIII GGCC 6 cut(s) 302, 328, 594, 615, 680, 830
HapII CCGG 4 cut(s) 151, 271, 340, 638
Hin1II CATG 8 cut(s) 19, 142, 262, 356, 548, 904, 941, 1275
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HpaII CCGG 4 cut(s) 151, 271, 340, 638
HphI GGTGA 6 cut(s) 31, 239, 296, 1110, 1274, 1298
Hpy166II GTNNAC 4 cut(s) 72, 109, 1265, 1474
Hpy188I TCNGA 9 cut(s) 429, 672, 825, 909, 916, 1051, 1129, 1177, 1432
Hpy188III TCNNGA 7 cut(s) 194, 307, 489, 689, 702, 976, 1111
Hpy8I GTNNAC 4 cut(s) 72, 109, 1265, 1474
HpyAV CCTTC 5 cut(s) 70, 490, 550, 557, 708
HpyCH4III ACNGT 2 cut(s) 1026, 1163
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 9 cut(s) 440, 472, 526, 542, 800, 869, 937, 1282, 1514
HpyF10VI GCNNNNNNNGC 6 cut(s) 94, 349, 523, 775, 784, 1152
HpyF3I CTNAG 5 cut(s) 457, 792, 906, 1050, 1302
HpySE526I ACGT 1 cut(s) 645
Hsp92II CATG 8 cut(s) 19, 142, 262, 356, 548, 904, 941, 1275
Ksp22I TGATCA 1 cut(s) 972
Kzo9I GATC 6 cut(s) 294, 820, 916, 972, 1013, 1478
LmnI GCTCC 2 cut(s) 85, 1103
Lsp1109I GCAGC 7 cut(s) 484, 784, 1107, 1158, 1161, 1164, 1295
LweI GCATC 2 cut(s) 924, 1523
MaeI CTAG 3 cut(s) 194, 435, 710
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 5 cut(s) 19, 227, 355, 1185, 1376
MalI GATC 6 cut(s) 296, 822, 918, 974, 1015, 1480
MboI GATC 6 cut(s) 294, 820, 916, 972, 1013, 1478
MfeI CAATTG 3 cut(s) 617, 808, 1217
MflI RGATCY 1 cut(s) 1478
MlsI TGGCCA 1 cut(s) 594
MluCI AATT 8 cut(s) 46, 177, 510, 617, 808, 851, 955, 1217
MluNI TGGCCA 1 cut(s) 594
MlyI GAGTC 6 cut(s) 462, 898, 1298, 1331, 1421, 1496
Mox20I TGGCCA 1 cut(s) 594
Mph1103I ATGCAT 1 cut(s) 939
MroXI GAANNNNTTC 1 cut(s) 732
MscI TGGCCA 1 cut(s) 594
MseI TTAA 7 cut(s) 176, 675, 782, 923, 963, 1106, 1437
MslI CAYNNNNRTG 2 cut(s) 881, 1415
Msp20I TGGCCA 1 cut(s) 594
MspA1I CMGCKG 1 cut(s) 475
MspCI CTTAAG 1 cut(s) 962
MspI CCGG 4 cut(s) 151, 271, 340, 638
MspR9I CCNGG 4 cut(s) 152, 272, 417, 682
MunI CAATTG 3 cut(s) 617, 808, 1217
Mva1269I GAATGC 2 cut(s) 871, 1211
MvaI CCWGG 2 cut(s) 417, 682
MvnI CGCG 1 cut(s) 1103
MwoI GCNNNNNNNGC 6 cut(s) 94, 349, 523, 775, 784, 1152
NciI CCSGG 2 cut(s) 152, 272
NcoI CCATGG 2 cut(s) 138, 258
NdeII GATC 6 cut(s) 294, 820, 916, 972, 1013, 1478
NlaIII CATG 8 cut(s) 19, 142, 262, 356, 548, 904, 941, 1275
NmeAIII GCCGAG 1 cut(s) 278
NmuCI GTSAC 4 cut(s) 19, 227, 355, 1376
NsiI ATGCAT 1 cut(s) 939
NspI RCATGY 3 cut(s) 356, 941, 1275
PaeI GCATGC 1 cut(s) 941
PaeR7I CTCGAG 1 cut(s) 1366
PceI AGGCCT 1 cut(s) 680
PctI GAATGC 2 cut(s) 871, 1211
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 5 cut(s) 412, 464, 485, 1172, 1358
PflMI CCANNNNNTGG 2 cut(s) 313, 590
PkrI GCNGC 7 cut(s) 474, 799, 1097, 1148, 1151, 1154, 1310
PleI GAGTC 6 cut(s) 461, 898, 1297, 1330, 1421, 1495
PpsI GAGTC 6 cut(s) 461, 898, 1297, 1330, 1421, 1495
PshBI ATTAAT 1 cut(s) 176
Psp6I CCWGG 2 cut(s) 415, 680
PspGI CCWGG 2 cut(s) 415, 680
PspPI GGNCC 1 cut(s) 327
PspXI VCTCGAGB 1 cut(s) 1366
PsuI RGATCY 1 cut(s) 1478
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 1 cut(s) 1372
RsaNI GTAC 1 cut(s) 1371
RseI CAYNNNNRTG 2 cut(s) 881, 1415
SaqAI TTAA 7 cut(s) 176, 675, 782, 923, 963, 1106, 1437
SatI GCNGC 7 cut(s) 473, 798, 1096, 1147, 1150, 1153, 1309
Sau3AI GATC 6 cut(s) 294, 820, 916, 972, 1013, 1478
Sau96I GGNCC 1 cut(s) 327
SchI GAGTC 6 cut(s) 462, 898, 1298, 1331, 1421, 1496
ScrFI CCNGG 4 cut(s) 152, 272, 417, 682
SfaNI GCATC 2 cut(s) 924, 1523
Sfr274I CTCGAG 1 cut(s) 1366
SlaI CTCGAG 1 cut(s) 1366
SmiMI CAYNNNNRTG 2 cut(s) 881, 1415
SmlI CTYRAG 2 cut(s) 962, 1366
SmoI CTYRAG 2 cut(s) 962, 1366
SphI GCATGC 1 cut(s) 941
Sse9I AATT 8 cut(s) 46, 177, 510, 617, 808, 851, 955, 1217
SseBI AGGCCT 1 cut(s) 680
SsiI CCGC 8 cut(s) 39, 247, 367, 941, 1011, 1101, 1326, 1484
SspI AATATT 1 cut(s) 570
SspMI CTAG 3 cut(s) 194, 435, 710
StuI AGGCCT 1 cut(s) 680
StyD4I CCNGG 4 cut(s) 150, 270, 415, 680
StyI CCWWGG 2 cut(s) 138, 258
TaaI ACNGT 2 cut(s) 1026, 1163
TaiI ACGT 1 cut(s) 648
TaqI TCGA 4 cut(s) 363, 483, 688, 1367
TasI AATT 8 cut(s) 46, 177, 510, 617, 808, 851, 955, 1217
TatI WGTACW 1 cut(s) 1370
TfiI GAWTC 5 cut(s) 412, 464, 485, 1172, 1358
Tru1I TTAA 7 cut(s) 176, 675, 782, 923, 963, 1106, 1437
Tru9I TTAA 7 cut(s) 176, 675, 782, 923, 963, 1106, 1437
TscAI CASTG 5 cut(s) 465, 746, 1168, 1310, 1402
TseFI GTSAC 4 cut(s) 19, 227, 355, 1376
TseI GCWGC 7 cut(s) 472, 797, 1095, 1146, 1149, 1152, 1308
Tsp45I GTSAC 4 cut(s) 19, 227, 355, 1376
TspDTI ATGAA 8 cut(s) 170, 644, 878, 968, 1035, 1152, 1185, 1473
TspRI CASTG 5 cut(s) 465, 746, 1168, 1310, 1402
Van91I CCANNNNNTGG 2 cut(s) 313, 590
Vha464I CTTAAG 1 cut(s) 962
VspI ATTAAT 1 cut(s) 176
XapI RAATTY 2 cut(s) 851, 955
XbaI TCTAGA 1 cut(s) 193
XceI RCATGY 3 cut(s) 356, 941, 1275
XhoI CTCGAG 1 cut(s) 1366
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 3 cut(s) 194, 435, 710
Zsp2I ATGCAT 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.