Rmu_sc0001444.1_g000012

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001444.1
OG0005120
Physical Location & Seq
Forward (+)
45400 .. 48960
3561 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1908 bp
atggcgcagaaccttctccgcctccggcgaagcctaagcagcagcctcgtcgcaaatctccaccgttctctatcgtccacctccgcactctcctctgtcccacgctcctcttccccttccccttcctcctcctcctcccccgccgttgttcaatctctccaattccgattccaatctcgatcattcaggtcgtcctccttctcttctgcacggccgccttacggtagcaacaaccaaaatgacgagattggccccgacaccatactcttcgaaggctgcgattacaaccactggcttatcgtcatggatttccccagagataacaagccttctcccgaagagatggtcagaacttatgaggaaacctgtgccagaggcttgaatatcagtgtggaagaggcaaaaaagaaaatttatgcttgtagcacaacaacgtatacaggctttcaggtgttgatgactgaggaagagtctgagaagtttaatggtgtacctggagttatttttgtgttgcccgattcttacattgatccacaaaacaaggagtatggaggagacaaatatgataatgggaaaattacacccagaccgcccccatttcagtatgagaggcagggctcaagataccgtgatcgtaacagatacgagcgacaaggagcaatgccaaatcagcaagggaatccctcatatggccaacagggttctccacaaggtggaggaaactataggacttcacaaaattatccacaccagacttatgccccaccaggacaaggggaacacagaggtcatacaccagttaattctcctggaggaagggacgcatatcagccaggtagaggtccaactccttcatatcagggcaattacaaccaacaggtgcaagggaacagaccacaaggagagcagagtacatatgctcctcctgatcaaggggaatataggggtaatgatgggacttattgttatgggcaagctccaagctctggaactcatggacatggtttgagtgggcttcaaggcccgggaacaggtggatcttatgggcaaggaccgagtggtggatcttatggacaaggaccaagtggtggaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatgggcaaggaccgagtgccccaggttatggtcaaggaccgagtgctggaagttatggccaaggatcgagatctggagcttctggacaaggacctgtatctagtgctcaagtccaaggacccagtgctggctaccctgggcatggagatcagagtttcacccaagaggagccaaggagaatgcaaggagaacaaacaaattatgcatacatgggacagacaggaactgaccaaggaagatattag

Protein Analysis

635

Amino Acids

65.0

Weight (kDa)

7.61

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 95 - 188 5.6e-37 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 437
AciI CCGC 5 cut(s) 19, 84, 141, 215, 590
AclWI GGATC 4 cut(s) 524, 1045, 1072, 1735
AcoI YGGCCR 3 cut(s) 212, 691, 1720
AcsI RAATTY 1 cut(s) 411
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 2 cut(s) 492, 913
AgsI TTSAA 3 cut(s) 152, 382, 1019
AluBI AGCT 3 cut(s) 977, 984, 1742
AluI AGCT 3 cut(s) 977, 984, 1742
Alw21I GWGCWC 1 cut(s) 1771
Alw26I GTCTC 1 cut(s) 549
AlwI GGATC 4 cut(s) 524, 1045, 1072, 1735
AlwNI CAGNNNCTG 1 cut(s) 1889
Ama87I CYCGRG 1 cut(s) 1024
AoxI GGCC 5 cut(s) 212, 250, 691, 1021, 1720
ApeKI GCWGC 3 cut(s) 39, 42, 276
ApoI RAATTY 1 cut(s) 411
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 2 cut(s) 1025, 1026
AsuHPI GGTGA 1 cut(s) 1813
AsuII TTCGAA 1 cut(s) 270
AvaI CYCGRG 1 cut(s) 1024
BalI TGGCCA 2 cut(s) 693, 1722
BanII GRGCYC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 1771
BbvI GCAGC 3 cut(s) 51, 54, 263
BccI CCATC 2 cut(s) 337, 947
BceAI ACGGC 2 cut(s) 128, 227
BcgI CGANNNNNNTGC 4 cut(s) 28, 31, 62, 65
BclI TGATCA 1 cut(s) 928
BcnI CCSGG 2 cut(s) 1025, 1026
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 1 cut(s) 1764
BfmI CTRYAG 1 cut(s) 724
BglII AGATCT 1 cut(s) 1733
BisI GCNGC 4 cut(s) 40, 43, 215, 277
BlsI GCNGC 4 cut(s) 41, 44, 216, 278
BmeT110I CYCGRG 1 cut(s) 1024
BmiI GGNNCC 3 cut(s) 253, 1783, 1833
BmrI ACTGGG 1 cut(s) 1779
BmuI ACTGGG 1 cut(s) 1779
BpmI CTGGAG 3 cut(s) 516, 831, 1758
Bpu10I CCTNAGC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 604, 1755
BpuMI CCSGG 2 cut(s) 1025, 1026
BsaBI GATNNNNATC 2 cut(s) 172, 1732
BsaXI ACNNNNNCTCC 2 cut(s) 904, 934
Bse1I ACTGG 3 cut(s) 296, 797, 1785
Bse3DI GCAATG 1 cut(s) 666
Bse8I GATNNNNATC 2 cut(s) 172, 1732
BseJI GATNNNNATC 2 cut(s) 172, 1732
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 2 cut(s) 453, 465
BseNI ACTGG 3 cut(s) 296, 797, 1785
BseRI GAGGAG 8 cut(s) 82, 97, 118, 121, 124, 567, 912, 1844
BseX3I CGGCCG 1 cut(s) 212
BseXI GCAGC 3 cut(s) 51, 54, 263
BsgI GTGCAG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 215
BshFI GGCC 5 cut(s) 214, 252, 693, 1023, 1722
BsiEI CGRYCG 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 1771
BsiHKCI CYCGRG 1 cut(s) 1024
BsiSI CCGG 2 cut(s) 25, 1025
BslFI GGGAC 4 cut(s) 83, 833, 970, 1890
BsmAI GTCTC 1 cut(s) 549
BsmFI GGGAC 4 cut(s) 83, 833, 970, 1890
BsmI GAATGC 1 cut(s) 1848
BsnI GGCC 5 cut(s) 214, 252, 693, 1023, 1722
BsoBI CYCGRG 1 cut(s) 1024
Bsp119I TTCGAA 1 cut(s) 270
Bsp143I GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1727, 1733, 1810
BspACI CCGC 5 cut(s) 19, 84, 141, 215, 590
BspANI GGCC 5 cut(s) 214, 252, 693, 1023, 1722
BspCNI CTCAG 2 cut(s) 454, 466
BspLI GGNNCC 3 cut(s) 253, 1783, 1833
BspPI GGATC 4 cut(s) 524, 1045, 1072, 1735
BspT104I TTCGAA 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 666
BsrI ACTGG 3 cut(s) 296, 797, 1785
BssMI GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1727, 1733, 1810
BssNAI GTATAC 1 cut(s) 438
BssT1I CCWWGG 4 cut(s) 1723, 1777, 1835, 1894
Bst1107I GTATAC 1 cut(s) 438
Bst4CI ACNGT 3 cut(s) 65, 224, 629
Bst6I CTCTTC 6 cut(s) 115, 208, 272, 333, 390, 462
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 2 cut(s) 975, 1792
BstDEI CTNAG 3 cut(s) 35, 462, 474
BstENI CCTNNNNNAGG 1 cut(s) 930
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 9 cut(s) 182, 532, 634, 931, 1040, 1067, 1730, 1736, 1813
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1727, 1733, 1810
BstMCI CGRYCG 1 cut(s) 215
BstMWI GCNNNNNNNGC 2 cut(s) 39, 670
BstSFI CTRYAG 1 cut(s) 724
BstV1I GCAGC 3 cut(s) 51, 54, 263
BstX2I RGATCY 3 cut(s) 1037, 1064, 1733
BstYI RGATCY 3 cut(s) 1037, 1064, 1733
BstZ17I GTATAC 1 cut(s) 438
BstZI CGGCCG 1 cut(s) 212
BsuRI GGCC 5 cut(s) 214, 252, 693, 1023, 1722
BtsIMutI CAGTG 3 cut(s) 289, 394, 1792
Cac8I GCNNGC 2 cut(s) 975, 1792
CaiI CAGNNNCTG 1 cut(s) 1889
CfoI GCGC 1 cut(s) 7
Cfr9I CCCGGG 1 cut(s) 1024
CseI GACGC 1 cut(s) 830
Csp6I GTAC 2 cut(s) 491, 912
CviAII CATG 5 cut(s) 304, 995, 1001, 1805, 1873
CviQI GTAC 2 cut(s) 491, 912
DdeI CTNAG 3 cut(s) 35, 462, 474
DpnI GATC 9 cut(s) 181, 531, 633, 930, 1039, 1066, 1729, 1735, 1812
DpnII GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1727, 1733, 1810
DraIII CACNNNGTG 1 cut(s) 713
EaeI YGGCCR 3 cut(s) 212, 691, 1720
EagI CGGCCG 1 cut(s) 212
Eam1104I CTCTTC 6 cut(s) 115, 208, 272, 333, 390, 462
EarI CTCTTC 6 cut(s) 115, 208, 272, 333, 390, 462
EciI GGCGGA 1 cut(s) 8
EclXI CGGCCG 1 cut(s) 212
Eco130I CCWWGG 4 cut(s) 1723, 1777, 1835, 1894
Eco24I GRGCYC 1 cut(s) 620
Eco52I CGGCCG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 1024
EcoNI CCTNNNNNAGG 1 cut(s) 930
EcoO109I RGGNCCY 2 cut(s) 1754, 1781
EcoT14I CCWWGG 4 cut(s) 1723, 1777, 1835, 1894
EcoT22I ATGCAT 1 cut(s) 1870
EcoT38I GRGCYC 1 cut(s) 620
ErhI CCWWGG 4 cut(s) 1723, 1777, 1835, 1894
FaeI CATG 5 cut(s) 307, 998, 1004, 1808, 1876
FaqI GGGAC 4 cut(s) 83, 833, 970, 1890
FatI CATG 5 cut(s) 303, 994, 1000, 1804, 1872
FauI CCCGC 1 cut(s) 148
FauNDI CATATG 2 cut(s) 688, 916
FbaI TGATCA 1 cut(s) 928
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 4 cut(s) 40, 43, 215, 277
FriOI GRGCYC 1 cut(s) 620
Fsp4HI GCNGC 4 cut(s) 40, 43, 215, 277
FspBI CTAG 1 cut(s) 1764
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 40, 43, 215, 277
GsuI CTGGAG 3 cut(s) 516, 831, 1758
HaeIII GGCC 5 cut(s) 214, 252, 693, 1023, 1722
HapII CCGG 2 cut(s) 25, 1025
HgaI GACGC 1 cut(s) 830
HhaI GCGC 1 cut(s) 7
Hin1II CATG 5 cut(s) 307, 998, 1004, 1808, 1876
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 4 cut(s) 168, 470, 518, 679
HpaII CCGG 2 cut(s) 25, 1025
HphI GGTGA 1 cut(s) 1813
Hpy166II GTNNAC 3 cut(s) 78, 438, 491
Hpy188I TCNGA 4 cut(s) 167, 350, 475, 1815
Hpy188III TCNNGA 8 cut(s) 177, 335, 621, 926, 987, 1731, 1737, 1746
Hpy8I GTNNAC 3 cut(s) 78, 438, 491
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 8 cut(s) 23, 126, 132, 208, 266, 339, 810, 861
HpyCH4III ACNGT 3 cut(s) 65, 224, 629
HpyCH4IV ACGT 1 cut(s) 434
HpyCH4V TGCA 4 cut(s) 209, 883, 1846, 1868
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 670
HpyF3I CTNAG 3 cut(s) 35, 462, 474
HpySE526I ACGT 1 cut(s) 434
Hsp92II CATG 5 cut(s) 307, 998, 1004, 1808, 1876
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 928
Kzo9I GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1727, 1733, 1810
LmnI GCTCC 6 cut(s) 110, 656, 925, 982, 1739, 1831
Lsp1109I GCAGC 3 cut(s) 51, 54, 263
MaeI CTAG 1 cut(s) 1764
MaeII ACGT 1 cut(s) 434
MaeIII GTNAC 1 cut(s) 635
MalI GATC 9 cut(s) 181, 531, 633, 930, 1039, 1066, 1729, 1735, 1812
MboI GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1727, 1733, 1810
MboII GAAGA 6 cut(s) 102, 195, 259, 350, 407, 479
MflI RGATCY 3 cut(s) 1037, 1064, 1733
MlsI TGGCCA 2 cut(s) 693, 1722
MluCI AATT 7 cut(s) 161, 411, 576, 739, 802, 865, 1861
MluNI TGGCCA 2 cut(s) 693, 1722
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 869
Mox20I TGGCCA 2 cut(s) 693, 1722
Mph1103I ATGCAT 1 cut(s) 1870
MscI TGGCCA 2 cut(s) 693, 1722
MseI TTAA 2 cut(s) 483, 801
MslI CAYNNNNRTG 1 cut(s) 999
Msp20I TGGCCA 2 cut(s) 693, 1722
MspI CCGG 2 cut(s) 25, 1025
Mva1269I GAATGC 1 cut(s) 1848
MwoI GCNNNNNNNGC 2 cut(s) 39, 670
NciI CCSGG 2 cut(s) 1025, 1026
NdeI CATATG 2 cut(s) 688, 916
NdeII GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1727, 1733, 1810
NlaIII CATG 5 cut(s) 307, 998, 1004, 1808, 1876
NlaIV GGNNCC 3 cut(s) 253, 1783, 1833
NsiI ATGCAT 1 cut(s) 1870
NspV TTCGAA 1 cut(s) 270
PasI CCCWGGG 1 cut(s) 1799
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 1848
PfeI GAWTC 3 cut(s) 168, 518, 679
PfoI TCCNGGA 1 cut(s) 808
PkrI GCNGC 4 cut(s) 41, 44, 216, 278
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PpuMI RGGWCCY 2 cut(s) 1754, 1781
Psp5II RGGWCCY 2 cut(s) 1754, 1781
PspN4I GGNNCC 3 cut(s) 253, 1783, 1833
PspPPI RGGWCCY 2 cut(s) 1754, 1781
PstNI CAGNNNCTG 1 cut(s) 1889
PsuI RGATCY 3 cut(s) 1037, 1064, 1733
RsaI GTAC 2 cut(s) 492, 913
RsaNI GTAC 2 cut(s) 491, 912
RseI CAYNNNNRTG 1 cut(s) 999
SaqAI TTAA 2 cut(s) 483, 801
SatI GCNGC 4 cut(s) 40, 43, 215, 277
Sau3AI GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1727, 1733, 1810
SchI GAGTC 1 cut(s) 479
SfcI CTRYAG 1 cut(s) 724
SfuI TTCGAA 1 cut(s) 270
SmaI CCCGGG 1 cut(s) 1026
SmiMI CAYNNNNRTG 1 cut(s) 999
SmlI CTYRAG 2 cut(s) 619, 1770
SmoI CTYRAG 2 cut(s) 619, 1770
Sse9I AATT 7 cut(s) 161, 411, 576, 739, 802, 865, 1861
SsiI CCGC 5 cut(s) 19, 84, 141, 215, 590
SspMI CTAG 1 cut(s) 1764
StyI CCWWGG 4 cut(s) 1723, 1777, 1835, 1894
TaaI ACNGT 3 cut(s) 65, 224, 629
TaiI ACGT 1 cut(s) 437
TaqI TCGA 3 cut(s) 178, 270, 1730
TasI AATT 7 cut(s) 161, 411, 576, 739, 802, 865, 1861
TatI WGTACW 1 cut(s) 911
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 3 cut(s) 168, 518, 679
Tru1I TTAA 2 cut(s) 483, 801
Tru9I TTAA 2 cut(s) 483, 801
TscAI CASTG 3 cut(s) 296, 394, 1792
TseI GCWGC 3 cut(s) 39, 42, 276
TspDTI ATGAA 1 cut(s) 843
TspMI CCCGGG 1 cut(s) 1024
TspRI CASTG 3 cut(s) 296, 394, 1792
XagI CCTNNNNNAGG 1 cut(s) 930
XapI RAATTY 1 cut(s) 411
XmaI CCCGGG 1 cut(s) 1024
XmiI GTMKAC 1 cut(s) 437
XspI CTAG 1 cut(s) 1764
Zsp2I ATGCAT 1 cut(s) 1870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.