Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 27, 108 |
| AclWI | GGATC | 1 cut(s) | 136 |
| AcoI | YGGCCR | 1 cut(s) | 112 |
| AcsI | RAATTY | 1 cut(s) | 167 |
| AgsI | TTSAA | 1 cut(s) | 328 |
| AluBI | AGCT | 1 cut(s) | 85 |
| AluI | AGCT | 1 cut(s) | 85 |
| Alw26I | GTCTC | 1 cut(s) | 181 |
| AlwI | GGATC | 1 cut(s) | 136 |
| AoxI | GGCC | 3 cut(s) | 57, 112, 189 |
| ApeKI | GCWGC | 1 cut(s) | 85 |
| ApoI | RAATTY | 1 cut(s) | 167 |
| AspS9I | GGNCC | 1 cut(s) | 58 |
| AsuHPI | GGTGA | 1 cut(s) | 41 |
| BalI | TGGCCA | 1 cut(s) | 114 |
| BbvI | GCAGC | 1 cut(s) | 72 |
| BccI | CCATC | 5 cut(s) | 215, 280, 292, 318, 382 |
| BceAI | ACGGC | 2 cut(s) | 67, 235 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 234, 268 |
| BcoDI | GTCTC | 1 cut(s) | 181 |
| BfaI | CTAG | 1 cut(s) | 356 |
| BglII | AGATCT | 1 cut(s) | 181 |
| BisI | GCNGC | 2 cut(s) | 86, 108 |
| BlsI | GCNGC | 2 cut(s) | 87, 109 |
| BmgT120I | GGNCC | 1 cut(s) | 58 |
| BpmI | CTGGAG | 1 cut(s) | 206 |
| BseGI | GGATG | 1 cut(s) | 369 |
| BseMII | CTCAG | 2 cut(s) | 174, 192 |
| BseXI | GCAGC | 1 cut(s) | 72 |
| BsgI | GTGCAG | 1 cut(s) | 71 |
| BshFI | GGCC | 3 cut(s) | 59, 114, 191 |
| BsiSI | CCGG | 1 cut(s) | 78 |
| BsmAI | GTCTC | 1 cut(s) | 181 |
| BsnI | GGCC | 3 cut(s) | 59, 114, 191 |
| Bsp143I | GATC | 2 cut(s) | 141, 181 |
| BspACI | CCGC | 2 cut(s) | 27, 108 |
| BspANI | GGCC | 3 cut(s) | 59, 114, 191 |
| BspCNI | CTCAG | 2 cut(s) | 173, 191 |
| BspPI | GGATC | 1 cut(s) | 136 |
| BssMI | GATC | 2 cut(s) | 141, 181 |
| Bst4CI | ACNGT | 1 cut(s) | 217 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 264 |
| BstC8I | GCNNGC | 2 cut(s) | 83, 112 |
| BstDEI | CTNAG | 2 cut(s) | 160, 178 |
| BstF5I | GGATG | 1 cut(s) | 369 |
| BstKTI | GATC | 2 cut(s) | 144, 184 |
| BstMAI | GTCTC | 1 cut(s) | 181 |
| BstMBI | GATC | 2 cut(s) | 141, 181 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 264 |
| BstV1I | GCAGC | 1 cut(s) | 72 |
| BstX2I | RGATCY | 1 cut(s) | 181 |
| BstYI | RGATCY | 1 cut(s) | 181 |
| BsuRI | GGCC | 3 cut(s) | 59, 114, 191 |
| BtsCI | GGATG | 1 cut(s) | 369 |
| Cac8I | GCNNGC | 2 cut(s) | 83, 112 |
| Cfr13I | GGNCC | 1 cut(s) | 58 |
| CviJI | RGCY | 6 cut(s) | 38, 59, 85, 114, 191, 250 |
| CviKI_1 | RGCY | 6 cut(s) | 38, 59, 85, 114, 191, 250 |
| DdeI | CTNAG | 2 cut(s) | 160, 178 |
| DpnI | GATC | 2 cut(s) | 143, 183 |
| DpnII | GATC | 2 cut(s) | 141, 181 |
| EaeI | YGGCCR | 1 cut(s) | 112 |
| FaiI | YATR | 5 cut(s) | 10, 72, 100, 117, 409 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 251, 283, 320, 352 |
| Fnu4HI | GCNGC | 2 cut(s) | 86, 108 |
| FokI | GGATG | 1 cut(s) | 356 |
| Fsp4HI | GCNGC | 2 cut(s) | 86, 108 |
| FspBI | CTAG | 1 cut(s) | 356 |
| GluI | GCNGC | 2 cut(s) | 86, 108 |
| GsuI | CTGGAG | 1 cut(s) | 206 |
| HaeIII | GGCC | 3 cut(s) | 59, 114, 191 |
| HapII | CCGG | 1 cut(s) | 78 |
| HinfI | GANTC | 3 cut(s) | 196, 290, 324 |
| HpaII | CCGG | 1 cut(s) | 78 |
| HphI | GGTGA | 1 cut(s) | 41 |
| Hpy188I | TCNGA | 6 cut(s) | 141, 154, 181, 280, 315, 379 |
| Hpy188III | TCNNGA | 2 cut(s) | 47, 185 |
| Hpy99I | CGWCG | 3 cut(s) | 53, 256, 284 |
| HpyAV | CCTTC | 1 cut(s) | 88 |
| HpyCH4III | ACNGT | 1 cut(s) | 217 |
| HpyCH4V | TGCA | 3 cut(s) | 88, 384, 391 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 264 |
| HpyF3I | CTNAG | 2 cut(s) | 160, 178 |
| Kzo9I | GATC | 2 cut(s) | 141, 181 |
| LpnPI | CCDG | 4 cut(s) | 91, 96, 147, 170 |
| Lsp1109I | GCAGC | 1 cut(s) | 72 |
| MaeI | CTAG | 1 cut(s) | 356 |
| MaeIII | GTNAC | 1 cut(s) | 44 |
| MalI | GATC | 2 cut(s) | 143, 183 |
| MboI | GATC | 2 cut(s) | 141, 181 |
| MflI | RGATCY | 1 cut(s) | 181 |
| MlsI | TGGCCA | 1 cut(s) | 114 |
| MluCI | AATT | 3 cut(s) | 167, 233, 318 |
| MluNI | TGGCCA | 1 cut(s) | 114 |
| MlyI | GAGTC | 2 cut(s) | 205, 299 |
| MmeI | TCCRAC | 2 cut(s) | 245, 303 |
| MnlI | CCTC | 5 cut(s) | 25, 74, 156, 181, 304 |
| Mox20I | TGGCCA | 1 cut(s) | 114 |
| MscI | TGGCCA | 1 cut(s) | 114 |
| MseI | TTAA | 1 cut(s) | 236 |
| Msp20I | TGGCCA | 1 cut(s) | 114 |
| MspA1I | CMGCKG | 1 cut(s) | 110 |
| MspI | CCGG | 1 cut(s) | 78 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 264 |
| NdeII | GATC | 2 cut(s) | 141, 181 |
| NmeAIII | GCCGAG | 1 cut(s) | 64 |
| NmuCI | GTSAC | 1 cut(s) | 44 |
| PfeI | GAWTC | 1 cut(s) | 324 |
| PkrI | GCNGC | 2 cut(s) | 87, 109 |
| PleI | GAGTC | 2 cut(s) | 204, 298 |
| PpsI | GAGTC | 2 cut(s) | 204, 298 |
| PspPI | GGNCC | 1 cut(s) | 58 |
| PsuI | RGATCY | 1 cut(s) | 181 |
| SaqAI | TTAA | 1 cut(s) | 236 |
| SatI | GCNGC | 2 cut(s) | 86, 108 |
| Sau3AI | GATC | 2 cut(s) | 141, 181 |
| Sau96I | GGNCC | 1 cut(s) | 58 |
| SchI | GAGTC | 2 cut(s) | 205, 299 |
| SetI | ASST | 5 cut(s) | 66, 87, 99, 166, 227 |
| Sse9I | AATT | 3 cut(s) | 167, 233, 318 |
| SsiI | CCGC | 2 cut(s) | 27, 108 |
| SspMI | CTAG | 1 cut(s) | 356 |
| TaaI | ACNGT | 1 cut(s) | 217 |
| TaqI | TCGA | 2 cut(s) | 254, 296 |
| TasI | AATT | 3 cut(s) | 167, 233, 318 |
| TauI | GCSGC | 1 cut(s) | 110 |
| TfiI | GAWTC | 1 cut(s) | 324 |
| Tru1I | TTAA | 1 cut(s) | 236 |
| Tru9I | TTAA | 1 cut(s) | 236 |
| TseFI | GTSAC | 1 cut(s) | 44 |
| TseI | GCWGC | 1 cut(s) | 85 |
| Tsp45I | GTSAC | 1 cut(s) | 44 |
| TspDTI | ATGAA | 3 cut(s) | 17, 337, 410 |
| TspGWI | ACGGA | 2 cut(s) | 56, 295 |
| XapI | RAATTY | 1 cut(s) | 167 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 317 |
| XspI | CTAG | 1 cut(s) | 356 |