Rmu_ssc0000454.1_g000013

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000454.1
OG0014862
Physical Location & Seq
Reverse (-)
50074 .. 50502
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000454.1_g000013.1.cds

Sequence Viewer

Length: 429 bp
atgcacttatgctgtgatgttagttgcggtgaggaagccgagtgccacgacggcgtggcccgcctccgtcatatcttcggcaagttgcacaagaaggtctggattgccgccggccatattctctctctctctctctctctgatccctctgtctgaaatcttaggtaaattccaagtctcagatctggaggccaagagtcgtgaaaccttctacatcgctctggcctgcaataaattcagagaagcaaagcagtcgagcaagttgtgcttggagaactctgacgagatggagtccctggcagtgtctgtccttcagagaattggattcaagttttgctttgggttttgcattttgctaggaaatgtcatcatcccatctaattgcattttgcagatgaaaaaggaaataaaaatagagaagaagaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

142

Amino Acids

15.95

Weight (kDa)

8.37

Isoelectric Point (pI)

65.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 27, 61, 108
AclWI GGATC 1 cut(s) 136
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 2 cut(s) 167, 233
AcuI CTGAAG 1 cut(s) 296
AgsI TTSAA 1 cut(s) 328
AjnI CCWGG 1 cut(s) 294
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 1 cut(s) 136
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 4 cut(s) 57, 112, 189, 222
ApoI RAATTY 2 cut(s) 167, 233
AspS9I GGNCC 1 cut(s) 58
AsuHPI GGTGA 1 cut(s) 41
BccI CCATC 2 cut(s) 280, 382
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciT130I CCWGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 1 cut(s) 356
BglI GCCNNNNNGGC 1 cut(s) 51
BglII AGATCT 1 cut(s) 181
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 296
BpmI CTGGAG 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 294
Bse118I RCCGGY 1 cut(s) 110
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 369
BseMII CTCAG 1 cut(s) 192
BshFI GGCC 4 cut(s) 59, 114, 191, 224
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 277
BsmAI GTCTC 1 cut(s) 181
BsmFI GGGAC 1 cut(s) 277
BsnI GGCC 4 cut(s) 59, 114, 191, 224
Bsp143I GATC 2 cut(s) 141, 181
BspACI CCGC 3 cut(s) 27, 61, 108
BspANI GGCC 4 cut(s) 59, 114, 191, 224
BspCNI CTCAG 1 cut(s) 191
BspPI GGATC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 2 cut(s) 141, 181
Bst2UI CCWGG 1 cut(s) 296
BstAPI GCANNNNNTGC 1 cut(s) 264
BstC8I GCNNGC 3 cut(s) 61, 112, 226
BstDEI CTNAG 2 cut(s) 160, 178
BstF5I GGATG 1 cut(s) 369
BstKTI GATC 2 cut(s) 144, 184
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 2 cut(s) 141, 181
BstMWI GCNNNNNNNGC 3 cut(s) 51, 60, 264
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 4 cut(s) 59, 114, 191, 224
BtgZI GCGATG 1 cut(s) 199
BtsCI GGATG 1 cut(s) 369
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 3 cut(s) 61, 112, 226
CaiI CAGNNNCTG 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 1 cut(s) 58
CviJI RGCY 5 cut(s) 38, 59, 114, 191, 224
CviKI_1 RGCY 5 cut(s) 38, 59, 114, 191, 224
DdeI CTNAG 2 cut(s) 160, 178
DpnI GATC 2 cut(s) 143, 183
DpnII GATC 2 cut(s) 141, 181
EaeI YGGCCR 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 296
EcoRII CCWGG 1 cut(s) 294
FaiI YATR 3 cut(s) 10, 72, 117
FalI AAGNNNNNCTT 4 cut(s) 251, 283, 320, 352
FaqI GGGAC 1 cut(s) 277
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 356
GluI GCNGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 4 cut(s) 59, 114, 191, 224
HapII CCGG 1 cut(s) 111
HinfI GANTC 3 cut(s) 196, 290, 324
HpaII CCGG 1 cut(s) 111
HphI GGTGA 1 cut(s) 41
Hpy188I TCNGA 6 cut(s) 141, 154, 181, 239, 280, 315
Hpy188III TCNNGA 3 cut(s) 100, 185, 200
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 3 cut(s) 88, 217, 320
HpyCH4V TGCA 6 cut(s) 4, 88, 228, 348, 384, 391
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 60, 264
HpyF3I CTNAG 2 cut(s) 160, 178
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Kzo9I GATC 2 cut(s) 141, 181
LpnPI CCDG 7 cut(s) 85, 124, 170, 206, 238, 281, 308
MaeI CTAG 1 cut(s) 356
MalI GATC 2 cut(s) 143, 183
MboI GATC 2 cut(s) 141, 181
MboII GAAGA 1 cut(s) 67
MflI RGATCY 1 cut(s) 181
MluCI AATT 4 cut(s) 167, 233, 318, 379
MlyI GAGTC 2 cut(s) 205, 299
MnlI CCTC 4 cut(s) 25, 74, 156, 181
MroNI GCCGGC 1 cut(s) 110
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 3 cut(s) 51, 60, 264
NaeI GCCGGC 1 cut(s) 112
NdeII GATC 2 cut(s) 141, 181
NgoMIV GCCGGC 1 cut(s) 110
NmeAIII GCCGAG 1 cut(s) 64
PdiI GCCGGC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 324
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 2 cut(s) 204, 298
PpsI GAGTC 2 cut(s) 204, 298
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspPI GGNCC 1 cut(s) 58
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 1 cut(s) 181
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 2 cut(s) 141, 181
Sau96I GGNCC 1 cut(s) 58
SchI GAGTC 2 cut(s) 205, 299
ScrFI CCNGG 1 cut(s) 296
SetI ASST 3 cut(s) 99, 166, 209
Sse9I AATT 4 cut(s) 167, 233, 318, 379
SsiI CCGC 3 cut(s) 27, 61, 108
SspMI CTAG 1 cut(s) 356
StyD4I CCNGG 1 cut(s) 294
TaqI TCGA 1 cut(s) 254
TasI AATT 4 cut(s) 167, 233, 318, 379
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 1 cut(s) 324
TscAI CASTG 1 cut(s) 306
TspDTI ATGAA 1 cut(s) 410
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 1 cut(s) 306
XapI RAATTY 2 cut(s) 167, 233
XspI CTAG 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.