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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 200 |
| AccI | GTMKAC | 1 cut(s) | 192 |
| AciI | CCGC | 4 cut(s) | 11, 45, 92, 198 |
| AclWI | GGATC | 1 cut(s) | 118 |
| AcoI | YGGCCR | 1 cut(s) | 96 |
| AcsI | RAATTY | 2 cut(s) | 149, 215 |
| AgeI | ACCGGT | 1 cut(s) | 280 |
| AgsI | TTSAA | 2 cut(s) | 253, 310 |
| AluBI | AGCT | 2 cut(s) | 22, 69 |
| AluI | AGCT | 2 cut(s) | 22, 69 |
| Alw26I | GTCTC | 1 cut(s) | 163 |
| AlwI | GGATC | 1 cut(s) | 118 |
| AoxI | GGCC | 4 cut(s) | 41, 96, 171, 204 |
| ApeKI | GCWGC | 1 cut(s) | 69 |
| ApoI | RAATTY | 2 cut(s) | 149, 215 |
| AsiGI | ACCGGT | 1 cut(s) | 280 |
| AspS9I | GGNCC | 1 cut(s) | 42 |
| AsuHPI | GGTGA | 1 cut(s) | 25 |
| BbvI | GCAGC | 1 cut(s) | 56 |
| BccI | CCATC | 2 cut(s) | 300, 364 |
| BceAI | ACGGC | 1 cut(s) | 51 |
| BcoDI | GTCTC | 1 cut(s) | 163 |
| BfaI | CTAG | 1 cut(s) | 338 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 35 |
| BisI | GCNGC | 2 cut(s) | 70, 92 |
| BlsI | GCNGC | 2 cut(s) | 71, 93 |
| BmgT120I | GGNCC | 1 cut(s) | 42 |
| BpmI | CTGGAG | 1 cut(s) | 188 |
| BsaWI | WCCGGW | 1 cut(s) | 280 |
| Bse118I | RCCGGY | 2 cut(s) | 94, 280 |
| BseMII | CTCAG | 2 cut(s) | 14, 156 |
| BseXI | GCAGC | 1 cut(s) | 56 |
| BsgI | GTGCAG | 1 cut(s) | 55 |
| BshFI | GGCC | 4 cut(s) | 43, 98, 173, 206 |
| BshTI | ACCGGT | 1 cut(s) | 280 |
| BsiSI | CCGG | 3 cut(s) | 95, 203, 281 |
| BslFI | GGGAC | 1 cut(s) | 259 |
| BsmAI | GTCTC | 1 cut(s) | 163 |
| BsmFI | GGGAC | 1 cut(s) | 259 |
| BsnI | GGCC | 4 cut(s) | 43, 98, 173, 206 |
| Bsp143I | GATC | 1 cut(s) | 123 |
| BspACI | CCGC | 4 cut(s) | 11, 45, 92, 198 |
| BspANI | GGCC | 4 cut(s) | 43, 98, 173, 206 |
| BspCNI | CTCAG | 2 cut(s) | 15, 155 |
| BspPI | GGATC | 1 cut(s) | 118 |
| BsrBI | CCGCTC | 1 cut(s) | 200 |
| BsrFI | RCCGGY | 2 cut(s) | 94, 280 |
| BssAI | RCCGGY | 2 cut(s) | 94, 280 |
| BssMI | GATC | 1 cut(s) | 123 |
| Bst4CI | ACNGT | 1 cut(s) | 190 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 246 |
| BstC8I | GCNNGC | 4 cut(s) | 45, 67, 96, 208 |
| BstDEI | CTNAG | 2 cut(s) | 23, 142 |
| BstKTI | GATC | 1 cut(s) | 126 |
| BstMAI | GTCTC | 1 cut(s) | 163 |
| BstMBI | GATC | 1 cut(s) | 123 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 35, 44, 246 |
| BstV1I | GCAGC | 1 cut(s) | 56 |
| BsuRI | GGCC | 4 cut(s) | 43, 98, 173, 206 |
| Cac8I | GCNNGC | 4 cut(s) | 45, 67, 96, 208 |
| Cfr10I | RCCGGY | 2 cut(s) | 94, 280 |
| Cfr13I | GGNCC | 1 cut(s) | 42 |
| CspAI | ACCGGT | 1 cut(s) | 280 |
| CviJI | RGCY | 6 cut(s) | 22, 43, 69, 98, 173, 206 |
| CviKI_1 | RGCY | 6 cut(s) | 22, 43, 69, 98, 173, 206 |
| DdeI | CTNAG | 2 cut(s) | 23, 142 |
| DpnI | GATC | 1 cut(s) | 125 |
| DpnII | GATC | 1 cut(s) | 123 |
| EaeI | YGGCCR | 1 cut(s) | 96 |
| FaiI | YATR | 2 cut(s) | 56, 101 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 233, 265, 302, 334 |
| FaqI | GGGAC | 1 cut(s) | 259 |
| FauI | CCCGC | 1 cut(s) | 52 |
| FblI | GTMKAC | 1 cut(s) | 192 |
| Fnu4HI | GCNGC | 2 cut(s) | 70, 92 |
| Fsp4HI | GCNGC | 2 cut(s) | 70, 92 |
| FspBI | CTAG | 1 cut(s) | 338 |
| GluI | GCNGC | 2 cut(s) | 70, 92 |
| GsuI | CTGGAG | 1 cut(s) | 188 |
| HaeIII | GGCC | 4 cut(s) | 43, 98, 173, 206 |
| HapII | CCGG | 3 cut(s) | 95, 203, 281 |
| HinfI | GANTC | 3 cut(s) | 178, 272, 306 |
| HpaII | CCGG | 3 cut(s) | 95, 203, 281 |
| HphI | GGTGA | 1 cut(s) | 25 |
| Hpy166II | GTNNAC | 1 cut(s) | 193 |
| Hpy188I | TCNGA | 5 cut(s) | 123, 136, 221, 262, 297 |
| Hpy188III | TCNNGA | 3 cut(s) | 84, 167, 182 |
| Hpy8I | GTNNAC | 1 cut(s) | 193 |
| Hpy99I | CGWCG | 2 cut(s) | 37, 266 |
| HpyAV | CCTTC | 1 cut(s) | 72 |
| HpyCH4III | ACNGT | 1 cut(s) | 190 |
| HpyCH4V | TGCA | 5 cut(s) | 72, 210, 330, 366, 373 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 35, 44, 246 |
| HpyF3I | CTNAG | 2 cut(s) | 23, 142 |
| KroI | GCCGGC | 1 cut(s) | 94 |
| KroNI | GCCGGC | 1 cut(s) | 96 |
| Kzo9I | GATC | 1 cut(s) | 123 |
| LmnI | GCTCC | 1 cut(s) | 205 |
| LpnPI | CCDG | 8 cut(s) | 47, 69, 108, 129, 152, 216, 220, 294 |
| Lsp1109I | GCAGC | 1 cut(s) | 56 |
| MaeI | CTAG | 1 cut(s) | 338 |
| MalI | GATC | 1 cut(s) | 125 |
| MbiI | CCGCTC | 1 cut(s) | 200 |
| MboI | GATC | 1 cut(s) | 123 |
| MboII | GAAGA | 1 cut(s) | 265 |
| MluCI | AATT | 4 cut(s) | 149, 215, 300, 361 |
| MlyI | GAGTC | 2 cut(s) | 187, 281 |
| MmeI | TCCRAC | 1 cut(s) | 285 |
| MnlI | CCTC | 3 cut(s) | 9, 58, 163 |
| MroNI | GCCGGC | 1 cut(s) | 94 |
| MspI | CCGG | 3 cut(s) | 95, 203, 281 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 35, 44, 246 |
| NaeI | GCCGGC | 1 cut(s) | 96 |
| NdeII | GATC | 1 cut(s) | 123 |
| NgoMIV | GCCGGC | 1 cut(s) | 94 |
| PdiI | GCCGGC | 1 cut(s) | 96 |
| PfeI | GAWTC | 1 cut(s) | 306 |
| PinAI | ACCGGT | 1 cut(s) | 280 |
| PkrI | GCNGC | 2 cut(s) | 71, 93 |
| PleI | GAGTC | 2 cut(s) | 186, 280 |
| PpsI | GAGTC | 2 cut(s) | 186, 280 |
| PspPI | GGNCC | 1 cut(s) | 42 |
| SatI | GCNGC | 2 cut(s) | 70, 92 |
| Sau3AI | GATC | 1 cut(s) | 123 |
| Sau96I | GGNCC | 1 cut(s) | 42 |
| SchI | GAGTC | 2 cut(s) | 187, 281 |
| SetI | ASST | 4 cut(s) | 24, 71, 83, 148 |
| SgrAI | CRCCGGYG | 1 cut(s) | 280 |
| Sse9I | AATT | 4 cut(s) | 149, 215, 300, 361 |
| SsiI | CCGC | 4 cut(s) | 11, 45, 92, 198 |
| SspMI | CTAG | 1 cut(s) | 338 |
| TaaI | ACNGT | 1 cut(s) | 190 |
| TasI | AATT | 4 cut(s) | 149, 215, 300, 361 |
| TauI | GCSGC | 1 cut(s) | 94 |
| TfiI | GAWTC | 1 cut(s) | 306 |
| TseI | GCWGC | 1 cut(s) | 69 |
| TspDTI | ATGAA | 1 cut(s) | 392 |
| TspGWI | ACGGA | 2 cut(s) | 40, 277 |
| XapI | RAATTY | 2 cut(s) | 149, 215 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 299 |
| XmiI | GTMKAC | 1 cut(s) | 192 |
| XspI | CTAG | 1 cut(s) | 338 |