Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 204 |
| AciI | CCGC | 3 cut(s) | 27, 108, 293 |
| AclWI | GGATC | 1 cut(s) | 130 |
| AcoI | YGGCCR | 1 cut(s) | 112 |
| AcsI | RAATTY | 2 cut(s) | 161, 227 |
| AgsI | TTSAA | 2 cut(s) | 232, 322 |
| AluBI | AGCT | 1 cut(s) | 85 |
| AluI | AGCT | 1 cut(s) | 85 |
| Alw26I | GTCTC | 2 cut(s) | 129, 175 |
| AlwI | GGATC | 1 cut(s) | 130 |
| AoxI | GGCC | 4 cut(s) | 57, 112, 183, 216 |
| ApeKI | GCWGC | 1 cut(s) | 85 |
| ApoI | RAATTY | 2 cut(s) | 161, 227 |
| AsuC2I | CCSGG | 1 cut(s) | 290 |
| AsuHPI | GGTGA | 2 cut(s) | 41, 206 |
| BbvI | GCAGC | 1 cut(s) | 72 |
| BccI | CCATC | 2 cut(s) | 274, 376 |
| BceAI | ACGGC | 1 cut(s) | 67 |
| BcnI | CCSGG | 1 cut(s) | 290 |
| BcoDI | GTCTC | 2 cut(s) | 129, 175 |
| BfaI | CTAG | 1 cut(s) | 350 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 51 |
| BglII | AGATCT | 1 cut(s) | 175 |
| BisI | GCNGC | 2 cut(s) | 86, 108 |
| BlsI | GCNGC | 2 cut(s) | 87, 109 |
| Bme1390I | CCNGG | 1 cut(s) | 290 |
| BmrFI | CCNGG | 1 cut(s) | 290 |
| BpmI | CTGGAG | 1 cut(s) | 200 |
| BpuMI | CCSGG | 1 cut(s) | 290 |
| BsaJI | CCNNGG | 1 cut(s) | 288 |
| Bse118I | RCCGGY | 1 cut(s) | 110 |
| BseDI | CCNNGG | 1 cut(s) | 288 |
| BseMII | CTCAG | 2 cut(s) | 168, 186 |
| BseXI | GCAGC | 1 cut(s) | 72 |
| BsgI | GTGCAG | 1 cut(s) | 71 |
| BshFI | GGCC | 4 cut(s) | 59, 114, 185, 218 |
| BsiSI | CCGG | 3 cut(s) | 78, 111, 290 |
| BslFI | GGGAC | 1 cut(s) | 271 |
| BsmAI | GTCTC | 2 cut(s) | 129, 175 |
| BsmFI | GGGAC | 1 cut(s) | 271 |
| BsnI | GGCC | 4 cut(s) | 59, 114, 185, 218 |
| Bsp143I | GATC | 2 cut(s) | 135, 175 |
| BspACI | CCGC | 3 cut(s) | 27, 108, 293 |
| BspANI | GGCC | 4 cut(s) | 59, 114, 185, 218 |
| BspCNI | CTCAG | 2 cut(s) | 167, 185 |
| BspPI | GGATC | 1 cut(s) | 130 |
| BsrFI | RCCGGY | 1 cut(s) | 110 |
| BssAI | RCCGGY | 1 cut(s) | 110 |
| BssECI | CCNNGG | 1 cut(s) | 288 |
| BssMI | GATC | 2 cut(s) | 135, 175 |
| Bst4CI | ACNGT | 3 cut(s) | 202, 211, 246 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 258 |
| BstC8I | GCNNGC | 3 cut(s) | 83, 112, 220 |
| BstDEI | CTNAG | 2 cut(s) | 154, 172 |
| BstKTI | GATC | 2 cut(s) | 138, 178 |
| BstMAI | GTCTC | 2 cut(s) | 129, 175 |
| BstMBI | GATC | 2 cut(s) | 135, 175 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 51, 258 |
| BstSCI | CCNGG | 1 cut(s) | 288 |
| BstV1I | GCAGC | 1 cut(s) | 72 |
| BstX2I | RGATCY | 1 cut(s) | 175 |
| BstYI | RGATCY | 1 cut(s) | 175 |
| BsuRI | GGCC | 4 cut(s) | 59, 114, 185, 218 |
| Cac8I | GCNNGC | 3 cut(s) | 83, 112, 220 |
| Cfr10I | RCCGGY | 1 cut(s) | 110 |
| CviJI | RGCY | 6 cut(s) | 38, 59, 85, 114, 185, 218 |
| CviKI_1 | RGCY | 6 cut(s) | 38, 59, 85, 114, 185, 218 |
| DdeI | CTNAG | 2 cut(s) | 154, 172 |
| DpnI | GATC | 2 cut(s) | 137, 177 |
| DpnII | GATC | 2 cut(s) | 135, 175 |
| EaeI | YGGCCR | 1 cut(s) | 112 |
| FaiI | YATR | 3 cut(s) | 10, 72, 117 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 245, 277, 314, 346 |
| FaqI | GGGAC | 1 cut(s) | 271 |
| FblI | GTMKAC | 1 cut(s) | 204 |
| Fnu4HI | GCNGC | 2 cut(s) | 86, 108 |
| Fsp4HI | GCNGC | 2 cut(s) | 86, 108 |
| FspBI | CTAG | 1 cut(s) | 350 |
| GluI | GCNGC | 2 cut(s) | 86, 108 |
| GsuI | CTGGAG | 1 cut(s) | 200 |
| HaeIII | GGCC | 4 cut(s) | 59, 114, 185, 218 |
| HapII | CCGG | 3 cut(s) | 78, 111, 290 |
| HinfI | GANTC | 2 cut(s) | 284, 318 |
| HpaII | CCGG | 3 cut(s) | 78, 111, 290 |
| HphI | GGTGA | 2 cut(s) | 41, 206 |
| Hpy166II | GTNNAC | 1 cut(s) | 205 |
| Hpy188I | TCNGA | 5 cut(s) | 135, 148, 175, 274, 373 |
| Hpy188III | TCNNGA | 2 cut(s) | 100, 179 |
| Hpy8I | GTNNAC | 1 cut(s) | 205 |
| Hpy99I | CGWCG | 2 cut(s) | 53, 278 |
| HpyAV | CCTTC | 2 cut(s) | 70, 88 |
| HpyCH4III | ACNGT | 3 cut(s) | 202, 211, 246 |
| HpyCH4V | TGCA | 5 cut(s) | 88, 222, 342, 378, 385 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 51, 258 |
| HpyF3I | CTNAG | 2 cut(s) | 154, 172 |
| KroI | GCCGGC | 1 cut(s) | 110 |
| KroNI | GCCGGC | 1 cut(s) | 112 |
| Kzo9I | GATC | 2 cut(s) | 135, 175 |
| LpnPI | CCDG | 9 cut(s) | 85, 91, 124, 141, 164, 200, 232, 303, 317 |
| Lsp1109I | GCAGC | 1 cut(s) | 72 |
| MaeI | CTAG | 1 cut(s) | 350 |
| MalI | GATC | 2 cut(s) | 137, 177 |
| MboI | GATC | 2 cut(s) | 135, 175 |
| MflI | RGATCY | 1 cut(s) | 175 |
| MluCI | AATT | 3 cut(s) | 161, 227, 312 |
| MlyI | GAGTC | 1 cut(s) | 293 |
| MmeI | TCCRAC | 1 cut(s) | 297 |
| MnlI | CCTC | 3 cut(s) | 25, 150, 175 |
| MroNI | GCCGGC | 1 cut(s) | 110 |
| MspI | CCGG | 3 cut(s) | 78, 111, 290 |
| MspR9I | CCNGG | 1 cut(s) | 290 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 51, 258 |
| NaeI | GCCGGC | 1 cut(s) | 112 |
| NciI | CCSGG | 1 cut(s) | 290 |
| NdeII | GATC | 2 cut(s) | 135, 175 |
| NgoMIV | GCCGGC | 1 cut(s) | 110 |
| NmeAIII | GCCGAG | 1 cut(s) | 64 |
| PdiI | GCCGGC | 1 cut(s) | 112 |
| PfeI | GAWTC | 1 cut(s) | 318 |
| PkrI | GCNGC | 2 cut(s) | 87, 109 |
| PleI | GAGTC | 1 cut(s) | 292 |
| PpsI | GAGTC | 1 cut(s) | 292 |
| PsuI | RGATCY | 1 cut(s) | 175 |
| SatI | GCNGC | 2 cut(s) | 86, 108 |
| Sau3AI | GATC | 2 cut(s) | 135, 175 |
| SchI | GAGTC | 1 cut(s) | 293 |
| ScrFI | CCNGG | 1 cut(s) | 290 |
| SetI | ASST | 3 cut(s) | 87, 99, 160 |
| Sse9I | AATT | 3 cut(s) | 161, 227, 312 |
| SsiI | CCGC | 3 cut(s) | 27, 108, 293 |
| SspMI | CTAG | 1 cut(s) | 350 |
| StyD4I | CCNGG | 1 cut(s) | 288 |
| TaaI | ACNGT | 3 cut(s) | 202, 211, 246 |
| TasI | AATT | 3 cut(s) | 161, 227, 312 |
| TauI | GCSGC | 1 cut(s) | 110 |
| TfiI | GAWTC | 1 cut(s) | 318 |
| TseI | GCWGC | 1 cut(s) | 85 |
| TspDTI | ATGAA | 2 cut(s) | 17, 404 |
| TspGWI | ACGGA | 1 cut(s) | 289 |
| XapI | RAATTY | 2 cut(s) | 161, 227 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 311 |
| XmiI | GTMKAC | 1 cut(s) | 204 |
| XspI | CTAG | 1 cut(s) | 350 |