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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 134 |
| AccI | GTMKAC | 1 cut(s) | 126 |
| AciI | CCGC | 4 cut(s) | 11, 45, 132, 215 |
| AcsI | RAATTY | 2 cut(s) | 83, 149 |
| AgsI | TTSAA | 2 cut(s) | 119, 244 |
| AluBI | AGCT | 2 cut(s) | 22, 69 |
| AluI | AGCT | 2 cut(s) | 22, 69 |
| Alw26I | GTCTC | 1 cut(s) | 97 |
| AoxI | GGCC | 3 cut(s) | 41, 105, 138 |
| ApeKI | GCWGC | 1 cut(s) | 69 |
| ApoI | RAATTY | 2 cut(s) | 83, 149 |
| AspS9I | GGNCC | 1 cut(s) | 42 |
| AsuHPI | GGTGA | 1 cut(s) | 25 |
| BbvI | GCAGC | 1 cut(s) | 56 |
| BccI | CCATC | 2 cut(s) | 234, 298 |
| BceAI | ACGGC | 2 cut(s) | 51, 151 |
| BcoDI | GTCTC | 1 cut(s) | 97 |
| BfaI | CTAG | 1 cut(s) | 272 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 35 |
| BisI | GCNGC | 1 cut(s) | 70 |
| BlsI | GCNGC | 1 cut(s) | 71 |
| BmgT120I | GGNCC | 1 cut(s) | 42 |
| BpmI | CTGGAG | 1 cut(s) | 122 |
| BseMII | CTCAG | 2 cut(s) | 14, 63 |
| BseXI | GCAGC | 1 cut(s) | 56 |
| BseYI | CCCAGC | 1 cut(s) | 211 |
| BsgI | GTGCAG | 1 cut(s) | 55 |
| BshFI | GGCC | 3 cut(s) | 43, 107, 140 |
| BsiSI | CCGG | 1 cut(s) | 137 |
| BslFI | GGGAC | 1 cut(s) | 193 |
| BsmAI | GTCTC | 1 cut(s) | 97 |
| BsmFI | GGGAC | 1 cut(s) | 193 |
| BsnI | GGCC | 3 cut(s) | 43, 107, 140 |
| BspACI | CCGC | 4 cut(s) | 11, 45, 132, 215 |
| BspANI | GGCC | 3 cut(s) | 43, 107, 140 |
| BspCNI | CTCAG | 2 cut(s) | 15, 62 |
| BsrBI | CCGCTC | 1 cut(s) | 134 |
| Bst4CI | ACNGT | 1 cut(s) | 124 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 180 |
| BstC8I | GCNNGC | 3 cut(s) | 45, 67, 142 |
| BstDEI | CTNAG | 2 cut(s) | 23, 49 |
| BstMAI | GTCTC | 1 cut(s) | 97 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 35, 44, 180 |
| BstV1I | GCAGC | 1 cut(s) | 56 |
| BsuRI | GGCC | 3 cut(s) | 43, 107, 140 |
| Cac8I | GCNNGC | 3 cut(s) | 45, 67, 142 |
| Cfr13I | GGNCC | 1 cut(s) | 42 |
| CviAII | CATG | 1 cut(s) | 31 |
| CviJI | RGCY | 6 cut(s) | 22, 43, 69, 107, 140, 166 |
| CviKI_1 | RGCY | 6 cut(s) | 22, 43, 69, 107, 140, 166 |
| DdeI | CTNAG | 2 cut(s) | 23, 49 |
| FaeI | CATG | 1 cut(s) | 34 |
| FaiI | YATR | 2 cut(s) | 32, 56 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 167, 199, 236, 268 |
| FaqI | GGGAC | 1 cut(s) | 193 |
| FatI | CATG | 1 cut(s) | 30 |
| FauI | CCCGC | 1 cut(s) | 52 |
| FblI | GTMKAC | 1 cut(s) | 126 |
| Fnu4HI | GCNGC | 1 cut(s) | 70 |
| Fsp4HI | GCNGC | 1 cut(s) | 70 |
| FspBI | CTAG | 1 cut(s) | 272 |
| GluI | GCNGC | 1 cut(s) | 70 |
| GsaI | CCCAGC | 1 cut(s) | 215 |
| GsuI | CTGGAG | 1 cut(s) | 122 |
| HaeIII | GGCC | 3 cut(s) | 43, 107, 140 |
| HapII | CCGG | 1 cut(s) | 137 |
| Hin1II | CATG | 1 cut(s) | 34 |
| HinfI | GANTC | 3 cut(s) | 112, 206, 240 |
| HpaII | CCGG | 1 cut(s) | 137 |
| HphI | GGTGA | 1 cut(s) | 25 |
| Hpy166II | GTNNAC | 1 cut(s) | 127 |
| Hpy188I | TCNGA | 3 cut(s) | 155, 196, 231 |
| Hpy188III | TCNNGA | 2 cut(s) | 101, 116 |
| Hpy8I | GTNNAC | 1 cut(s) | 127 |
| HpyCH4III | ACNGT | 1 cut(s) | 124 |
| HpyCH4V | TGCA | 5 cut(s) | 72, 144, 264, 300, 307 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 35, 44, 180 |
| HpyF3I | CTNAG | 2 cut(s) | 23, 49 |
| Hsp92II | CATG | 1 cut(s) | 34 |
| LmnI | GCTCC | 1 cut(s) | 139 |
| LpnPI | CCDG | 5 cut(s) | 47, 86, 150, 154, 225 |
| Lsp1109I | GCAGC | 1 cut(s) | 56 |
| MaeI | CTAG | 1 cut(s) | 272 |
| MbiI | CCGCTC | 1 cut(s) | 134 |
| MluCI | AATT | 4 cut(s) | 83, 149, 234, 295 |
| MlyI | GAGTC | 2 cut(s) | 121, 215 |
| MnlI | CCTC | 3 cut(s) | 9, 58, 97 |
| MspA1I | CMGCKG | 1 cut(s) | 215 |
| MspI | CCGG | 1 cut(s) | 137 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 35, 44, 180 |
| NlaIII | CATG | 1 cut(s) | 34 |
| PfeI | GAWTC | 1 cut(s) | 240 |
| PkrI | GCNGC | 1 cut(s) | 71 |
| PleI | GAGTC | 2 cut(s) | 120, 214 |
| PpsI | GAGTC | 2 cut(s) | 120, 214 |
| PspFI | CCCAGC | 1 cut(s) | 211 |
| PspPI | GGNCC | 1 cut(s) | 42 |
| SatI | GCNGC | 1 cut(s) | 70 |
| Sau96I | GGNCC | 1 cut(s) | 42 |
| SchI | GAGTC | 2 cut(s) | 121, 215 |
| SetI | ASST | 2 cut(s) | 24, 71 |
| Sse9I | AATT | 4 cut(s) | 83, 149, 234, 295 |
| SsiI | CCGC | 4 cut(s) | 11, 45, 132, 215 |
| SspMI | CTAG | 1 cut(s) | 272 |
| TaaI | ACNGT | 1 cut(s) | 124 |
| TasI | AATT | 4 cut(s) | 83, 149, 234, 295 |
| TfiI | GAWTC | 1 cut(s) | 240 |
| TseI | GCWGC | 1 cut(s) | 69 |
| TspDTI | ATGAA | 1 cut(s) | 326 |
| TspGWI | ACGGA | 1 cut(s) | 211 |
| XapI | RAATTY | 2 cut(s) | 83, 149 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 233 |
| XmiI | GTMKAC | 1 cut(s) | 126 |
| XspI | CTAG | 1 cut(s) | 272 |