Rmu_sc0012554.1_g000006

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012554.1
OG0014862
Physical Location & Seq
Forward (+)
22870 .. 25982
3113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012554.1_g000006.1.cds

Sequence Viewer

Length: 741 bp
atgaacttatgctgtgatgttagttgcggtgaggaagctgagtgccacgacggcgtggcccgcctccgtcatatctctggcaagctgcacaaaaaggtctggattgtcgcccgccatattctctgtctctgtctctctctgtctctctctctgatccctttatctgaaatctcaggtaaattccaagtctcaaatctggaggccaagagtcttgaaaccgtctacaccgctccggcctgcaataaattcagagaagcaaagccgttgaacaagttgtgcctggagaactccgacgagatagagtccccagcgcttgctgggcgtgcgcaggtggggtggcgctgggatgagcagacgttaggttgcgcaagacgtgggagggggactaggagcgggtggaggctagatcaaaggctgggaggctccggtgaggaggccggaatgcaggcggttgggatcagtgctgcagatgactccgatgaaggggaggtgggtgctgcagttccttatgggctggcggggacgaaagacgatctgacgccggaatccggtcaacagtccagtagccagattccggggaccggttgcaggattccggtcattggtagccggattctggtcacgggtcaccggatttcagtcatcggtcaccggagtccggtgaccggtcccaggagtccgaggccggccactggtcaccggagcacagtaaggtggagggtgacttttctctctaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

246

Amino Acids

26.45

Weight (kDa)

8.72

Isoelectric Point (pI)

61.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 319
Acc16I TGCGCA 2 cut(s) 327, 367
Acc36I ACCTGC 1 cut(s) 319
AccBSI CCGCTC 2 cut(s) 230, 393
AccI GTMKAC 1 cut(s) 222
AciI CCGC 7 cut(s) 27, 61, 112, 228, 393, 449, 518
AclWI GGATC 2 cut(s) 148, 464
AcoI YGGCCR 1 cut(s) 687
AcsI RAATTY 2 cut(s) 179, 245
AcyI GRCGYC 1 cut(s) 539
AfeI AGCGCT 1 cut(s) 312
AgeI ACCGGT 2 cut(s) 581, 665
AgsI TTSAA 2 cut(s) 215, 268
AjiI CACGTC 1 cut(s) 374
AjnI CCWGG 2 cut(s) 279, 671
AluBI AGCT 2 cut(s) 38, 85
AluI AGCT 2 cut(s) 38, 85
Alw21I GWGCWC 1 cut(s) 707
Alw26I GTCTC 4 cut(s) 131, 137, 147, 193
AlwI GGATC 2 cut(s) 148, 464
Aor51HI AGCGCT 1 cut(s) 312
AoxI GGCC 6 cut(s) 57, 201, 234, 435, 683, 687
ApeKI GCWGC 3 cut(s) 85, 464, 497
ApoI RAATTY 2 cut(s) 179, 245
AsiGI ACCGGT 2 cut(s) 581, 665
AspLEI GCGC 4 cut(s) 313, 328, 342, 368
AspS9I GGNCC 3 cut(s) 58, 579, 668
AsuC2I CCSGG 1 cut(s) 576
AsuHPI GGTGA 7 cut(s) 41, 440, 620, 641, 673, 689, 733
AvaII GGWCC 2 cut(s) 579, 668
Bbv12I GWGCWC 1 cut(s) 707
BbvI GCAGC 3 cut(s) 72, 451, 484
BceAI ACGGC 2 cut(s) 67, 247
BciT130I CCWGG 2 cut(s) 281, 673
BcnI CCSGG 1 cut(s) 576
BcoDI GTCTC 4 cut(s) 131, 137, 147, 193
BfaI CTAG 2 cut(s) 387, 404
BfmI CTRYAG 2 cut(s) 465, 498
BfoI RGCGCY 2 cut(s) 314, 343
BfuAI ACCTGC 1 cut(s) 319
BglI GCCNNNNNGGC 1 cut(s) 51
BisI GCNGC 3 cut(s) 86, 465, 498
BlsI GCNGC 3 cut(s) 87, 466, 499
Bme1390I CCNGG 3 cut(s) 281, 576, 673
Bme18I GGWCC 2 cut(s) 579, 668
BmgBI CACGTC 1 cut(s) 374
BmgT120I GGNCC 3 cut(s) 58, 579, 668
BmiI GGNNCC 3 cut(s) 424, 580, 670
BmrFI CCNGG 3 cut(s) 281, 576, 673
BpmI CTGGAG 2 cut(s) 218, 302
BpuMI CCSGG 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 539
BsaJI CCNNGG 3 cut(s) 575, 671, 680
BsaWI WCCGGW 9 cut(s) 425, 548, 581, 595, 630, 651, 658, 665, 699
Bse118I RCCGGY 3 cut(s) 581, 665, 685
Bse1I ACTGG 2 cut(s) 561, 697
BseBI CCWGG 2 cut(s) 281, 673
BseDI CCNNGG 3 cut(s) 575, 671, 680
BseGI GGATG 1 cut(s) 352
BseMII CTCAG 2 cut(s) 30, 186
BseNI ACTGG 2 cut(s) 561, 697
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 3 cut(s) 72, 451, 484
BseYI CCCAGC 4 cut(s) 307, 317, 342, 415
BsgI GTGCAG 1 cut(s) 71
BshFI GGCC 6 cut(s) 59, 203, 236, 437, 685, 689
BshTI ACCGGT 2 cut(s) 581, 665
BsiHKAI GWGCWC 1 cut(s) 707
BslFI GGGAC 5 cut(s) 289, 397, 535, 592, 654
BsmAI GTCTC 4 cut(s) 131, 137, 147, 193
BsmFI GGGAC 5 cut(s) 289, 397, 535, 592, 654
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 6 cut(s) 59, 203, 236, 437, 685, 689
Bsp1286I GDGCHC 1 cut(s) 707
Bsp143I GATC 4 cut(s) 153, 406, 456, 532
BspACI CCGC 7 cut(s) 27, 61, 112, 228, 393, 449, 518
BspANI GGCC 6 cut(s) 59, 203, 236, 437, 685, 689
BspCNI CTCAG 2 cut(s) 31, 185
BspLI GGNNCC 3 cut(s) 424, 580, 670
BspMAI CTGCAG 2 cut(s) 469, 502
BspMI ACCTGC 1 cut(s) 319
BspPI GGATC 2 cut(s) 148, 464
BsrBI CCGCTC 2 cut(s) 230, 393
BsrFI RCCGGY 3 cut(s) 581, 665, 685
BsrI ACTGG 2 cut(s) 561, 697
BssAI RCCGGY 3 cut(s) 581, 665, 685
BssECI CCNNGG 3 cut(s) 575, 671, 680
BssMI GATC 4 cut(s) 153, 406, 456, 532
BssNI GRCGYC 1 cut(s) 539
Bst2UI CCWGG 2 cut(s) 281, 673
Bst4CI ACNGT 3 cut(s) 220, 558, 709
BstACI GRCGYC 1 cut(s) 539
BstC8I GCNNGC 9 cut(s) 61, 83, 112, 238, 315, 324, 447, 516, 687
BstDEI CTNAG 3 cut(s) 39, 172, 735
BstEII GGTNACC 4 cut(s) 626, 647, 661, 695
BstF5I GGATG 1 cut(s) 352
BstH2I RGCGCY 2 cut(s) 314, 343
BstHHI GCGC 4 cut(s) 313, 328, 342, 368
BstKTI GATC 4 cut(s) 156, 409, 459, 535
BstMAI GTCTC 4 cut(s) 131, 137, 147, 193
BstMBI GATC 4 cut(s) 153, 406, 456, 532
BstMWI GCNNNNNNNGC 4 cut(s) 51, 60, 319, 323
BstNI CCWGG 2 cut(s) 281, 673
BstPI GGTNACC 4 cut(s) 626, 647, 661, 695
BstSCI CCNGG 3 cut(s) 279, 574, 671
BstSFI CTRYAG 2 cut(s) 465, 498
BstV1I GCAGC 3 cut(s) 72, 451, 484
BsuRI GGCC 6 cut(s) 59, 203, 236, 437, 685, 689
BtrI CACGTC 1 cut(s) 374
BtsCI GGATG 1 cut(s) 352
BtsIMutI CAGTG 2 cut(s) 466, 690
BveI ACCTGC 1 cut(s) 319
Cac8I GCNNGC 9 cut(s) 61, 83, 112, 238, 315, 324, 447, 516, 687
CfoI GCGC 4 cut(s) 313, 328, 342, 368
Cfr10I RCCGGY 3 cut(s) 581, 665, 685
Cfr13I GGNCC 3 cut(s) 58, 579, 668
CseI GACGC 1 cut(s) 547
CspAI ACCGGT 2 cut(s) 581, 665
DdeI CTNAG 3 cut(s) 39, 172, 735
DpnI GATC 4 cut(s) 155, 408, 458, 534
DpnII GATC 4 cut(s) 153, 406, 456, 532
EaeI YGGCCR 1 cut(s) 687
Eco47I GGWCC 2 cut(s) 579, 668
Eco47III AGCGCT 1 cut(s) 312
Eco91I GGTNACC 4 cut(s) 626, 647, 661, 695
EcoO65I GGTNACC 4 cut(s) 626, 647, 661, 695
EcoRII CCWGG 2 cut(s) 279, 671
FaiI YATR 4 cut(s) 10, 72, 117, 510
FaqI GGGAC 5 cut(s) 289, 397, 535, 592, 654
FauI CCCGC 4 cut(s) 68, 119, 386, 511
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 3 cut(s) 86, 465, 498
FokI GGATG 1 cut(s) 359
FseI GGCCGGCC 1 cut(s) 689
Fsp4HI GCNGC 3 cut(s) 86, 465, 498
FspBI CTAG 2 cut(s) 387, 404
FspI TGCGCA 2 cut(s) 327, 367
GlaI GCGC 4 cut(s) 312, 327, 341, 367
GluI GCNGC 3 cut(s) 86, 465, 498
GsaI CCCAGC 4 cut(s) 311, 321, 346, 419
GsuI CTGGAG 2 cut(s) 218, 302
HaeII RGCGCY 2 cut(s) 314, 343
HaeIII GGCC 6 cut(s) 59, 203, 236, 437, 685, 689
HgaI GACGC 1 cut(s) 547
HhaI GCGC 4 cut(s) 313, 328, 342, 368
Hin1I GRCGYC 1 cut(s) 539
Hin6I GCGC 4 cut(s) 311, 326, 340, 366
HinP1I GCGC 4 cut(s) 311, 326, 340, 366
HincII GTYRAC 1 cut(s) 554
HindII GTYRAC 1 cut(s) 554
HinfI GANTC 9 cut(s) 208, 302, 473, 545, 571, 592, 613, 655, 676
HphI GGTGA 7 cut(s) 41, 440, 620, 641, 673, 689, 733
Hpy166II GTNNAC 2 cut(s) 223, 554
Hpy188I TCNGA 7 cut(s) 153, 166, 251, 292, 478, 537, 681
Hpy188III TCNNGA 3 cut(s) 100, 197, 212
Hpy8I GTNNAC 2 cut(s) 223, 554
Hpy99I CGWCG 2 cut(s) 53, 296
HpyAV CCTTC 1 cut(s) 476
HpyCH4III ACNGT 3 cut(s) 220, 558, 709
HpyCH4IV ACGT 2 cut(s) 356, 373
HpyCH4V TGCA 6 cut(s) 88, 240, 445, 467, 500, 588
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 60, 319, 323
HpyF3I CTNAG 3 cut(s) 39, 172, 735
HpySE526I ACGT 2 cut(s) 356, 373
Hsp92I GRCGYC 1 cut(s) 539
HspAI GCGC 4 cut(s) 311, 326, 340, 366
KroI GCCGGC 1 cut(s) 685
KroNI GCCGGC 1 cut(s) 687
Kzo9I GATC 4 cut(s) 153, 406, 456, 532
LmnI GCTCC 4 cut(s) 235, 390, 428, 702
Lsp1109I GCAGC 3 cut(s) 72, 451, 484
MaeI CTAG 2 cut(s) 387, 404
MaeII ACGT 2 cut(s) 356, 373
MaeIII GTNAC 6 cut(s) 619, 626, 647, 661, 695, 721
MalI GATC 4 cut(s) 155, 408, 458, 534
MbiI CCGCTC 2 cut(s) 230, 393
MboI GATC 4 cut(s) 153, 406, 456, 532
MhlI GDGCHC 1 cut(s) 707
MluCI AATT 2 cut(s) 179, 245
MlyI GAGTC 5 cut(s) 217, 311, 467, 664, 685
MmeI TCCRAC 1 cut(s) 315
MroNI GCCGGC 1 cut(s) 685
MspR9I CCNGG 3 cut(s) 281, 576, 673
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 2 cut(s) 281, 673
MwoI GCNNNNNNNGC 4 cut(s) 51, 60, 319, 323
NaeI GCCGGC 1 cut(s) 687
NciI CCSGG 1 cut(s) 576
NdeII GATC 4 cut(s) 153, 406, 456, 532
NgoMIV GCCGGC 1 cut(s) 685
NlaIV GGNNCC 3 cut(s) 424, 580, 670
NmuCI GTSAC 6 cut(s) 619, 626, 647, 661, 695, 721
NsbI TGCGCA 2 cut(s) 327, 367
PaqCI CACCTGC 1 cut(s) 319
PctI GAATGC 1 cut(s) 447
PdiI GCCGGC 1 cut(s) 687
PfeI GAWTC 4 cut(s) 545, 571, 592, 613
PinAI ACCGGT 2 cut(s) 581, 665
PkrI GCNGC 3 cut(s) 87, 466, 499
PleI GAGTC 5 cut(s) 216, 310, 467, 663, 684
PpsI GAGTC 5 cut(s) 216, 310, 467, 663, 684
Psp6I CCWGG 2 cut(s) 279, 671
PspEI GGTNACC 4 cut(s) 626, 647, 661, 695
PspFI CCCAGC 4 cut(s) 307, 317, 342, 415
PspGI CCWGG 2 cut(s) 279, 671
PspN4I GGNNCC 3 cut(s) 424, 580, 670
PspPI GGNCC 3 cut(s) 58, 579, 668
PstI CTGCAG 2 cut(s) 469, 502
RigI GGCCGGCC 1 cut(s) 689
SatI GCNGC 3 cut(s) 86, 465, 498
Sau3AI GATC 4 cut(s) 153, 406, 456, 532
Sau96I GGNCC 3 cut(s) 58, 579, 668
SchI GAGTC 5 cut(s) 217, 311, 467, 664, 685
ScrFI CCNGG 3 cut(s) 281, 576, 673
SduI GDGCHC 1 cut(s) 707
SfcI CTRYAG 2 cut(s) 465, 498
SinI GGWCC 2 cut(s) 579, 668
Sse9I AATT 2 cut(s) 179, 245
SsiI CCGC 7 cut(s) 27, 61, 112, 228, 393, 449, 518
SspMI CTAG 2 cut(s) 387, 404
StyD4I CCNGG 3 cut(s) 279, 574, 671
TaaI ACNGT 3 cut(s) 220, 558, 709
TaiI ACGT 2 cut(s) 359, 376
TaqII GACCGA 1 cut(s) 635
TasI AATT 2 cut(s) 179, 245
TfiI GAWTC 4 cut(s) 545, 571, 592, 613
TscAI CASTG 2 cut(s) 466, 697
TseFI GTSAC 6 cut(s) 619, 626, 647, 661, 695, 721
TseI GCWGC 3 cut(s) 85, 464, 497
Tsp45I GTSAC 6 cut(s) 619, 626, 647, 661, 695, 721
TspDTI ATGAA 2 cut(s) 17, 495
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 2 cut(s) 466, 697
VpaK11BI GGWCC 2 cut(s) 579, 668
XapI RAATTY 2 cut(s) 179, 245
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 2 cut(s) 387, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.