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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 218 |
| AciI | CCGC | 5 cut(s) | 11, 92, 207, 216, 299 |
| AclWI | GGATC | 1 cut(s) | 136 |
| AcoI | YGGCCR | 1 cut(s) | 96 |
| AcsI | RAATTY | 2 cut(s) | 167, 233 |
| AgsI | TTSAA | 1 cut(s) | 328 |
| AluBI | AGCT | 1 cut(s) | 69 |
| AluI | AGCT | 1 cut(s) | 69 |
| Alw26I | GTCTC | 2 cut(s) | 119, 181 |
| AlwI | GGATC | 1 cut(s) | 136 |
| AoxI | GGCC | 4 cut(s) | 41, 96, 189, 222 |
| ApeKI | GCWGC | 1 cut(s) | 69 |
| ApoI | RAATTY | 2 cut(s) | 167, 233 |
| AspS9I | GGNCC | 1 cut(s) | 42 |
| AsuHPI | GGTGA | 1 cut(s) | 25 |
| BbvI | GCAGC | 1 cut(s) | 56 |
| BccI | CCATC | 3 cut(s) | 280, 318, 382 |
| BceAI | ACGGC | 2 cut(s) | 51, 235 |
| BcoDI | GTCTC | 2 cut(s) | 119, 181 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 35 |
| BisI | GCNGC | 2 cut(s) | 70, 92 |
| BlsI | GCNGC | 2 cut(s) | 71, 93 |
| BmgT120I | GGNCC | 1 cut(s) | 42 |
| BmiI | GGNNCC | 1 cut(s) | 44 |
| Bse118I | RCCGGY | 1 cut(s) | 94 |
| BseMII | CTCAG | 1 cut(s) | 174 |
| BseXI | GCAGC | 1 cut(s) | 56 |
| BseYI | CCCAGC | 1 cut(s) | 295 |
| BsgI | GTGCAG | 1 cut(s) | 55 |
| BshFI | GGCC | 4 cut(s) | 43, 98, 191, 224 |
| BsiSI | CCGG | 2 cut(s) | 95, 221 |
| BslFI | GGGAC | 1 cut(s) | 277 |
| BsmAI | GTCTC | 2 cut(s) | 119, 181 |
| BsmFI | GGGAC | 1 cut(s) | 277 |
| BsnI | GGCC | 4 cut(s) | 43, 98, 191, 224 |
| Bsp143I | GATC | 1 cut(s) | 141 |
| BspACI | CCGC | 5 cut(s) | 11, 92, 207, 216, 299 |
| BspANI | GGCC | 4 cut(s) | 43, 98, 191, 224 |
| BspCNI | CTCAG | 1 cut(s) | 173 |
| BspLI | GGNNCC | 1 cut(s) | 44 |
| BspPI | GGATC | 1 cut(s) | 136 |
| BsrBI | CCGCTC | 1 cut(s) | 218 |
| BsrFI | RCCGGY | 1 cut(s) | 94 |
| BssAI | RCCGGY | 1 cut(s) | 94 |
| BssMI | GATC | 1 cut(s) | 141 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 264 |
| BstC8I | GCNNGC | 3 cut(s) | 67, 96, 226 |
| BstDEI | CTNAG | 2 cut(s) | 160, 356 |
| BstKTI | GATC | 1 cut(s) | 144 |
| BstMAI | GTCTC | 2 cut(s) | 119, 181 |
| BstMBI | GATC | 1 cut(s) | 141 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 35, 215, 264 |
| BstV1I | GCAGC | 1 cut(s) | 56 |
| BsuRI | GGCC | 4 cut(s) | 43, 98, 191, 224 |
| Cac8I | GCNNGC | 3 cut(s) | 67, 96, 226 |
| Cfr10I | RCCGGY | 1 cut(s) | 94 |
| Cfr13I | GGNCC | 1 cut(s) | 42 |
| CviJI | RGCY | 7 cut(s) | 22, 43, 69, 98, 191, 224, 250 |
| CviKI_1 | RGCY | 7 cut(s) | 22, 43, 69, 98, 191, 224, 250 |
| DdeI | CTNAG | 2 cut(s) | 160, 356 |
| DpnI | GATC | 1 cut(s) | 143 |
| DpnII | GATC | 1 cut(s) | 141 |
| EaeI | YGGCCR | 1 cut(s) | 96 |
| FaiI | YATR | 3 cut(s) | 56, 101, 389 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 251, 283, 320, 352 |
| FaqI | GGGAC | 1 cut(s) | 277 |
| Fnu4HI | GCNGC | 2 cut(s) | 70, 92 |
| Fsp4HI | GCNGC | 2 cut(s) | 70, 92 |
| GluI | GCNGC | 2 cut(s) | 70, 92 |
| GsaI | CCCAGC | 1 cut(s) | 299 |
| HaeIII | GGCC | 4 cut(s) | 43, 98, 191, 224 |
| HapII | CCGG | 2 cut(s) | 95, 221 |
| HinfI | GANTC | 3 cut(s) | 196, 290, 324 |
| HpaII | CCGG | 2 cut(s) | 95, 221 |
| HphI | GGTGA | 1 cut(s) | 25 |
| Hpy188I | TCNGA | 4 cut(s) | 154, 239, 280, 315 |
| Hpy188III | TCNNGA | 3 cut(s) | 84, 185, 200 |
| Hpy99I | CGWCG | 2 cut(s) | 37, 284 |
| HpyAV | CCTTC | 1 cut(s) | 72 |
| HpyCH4V | TGCA | 4 cut(s) | 72, 228, 348, 384 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 35, 215, 264 |
| HpyF3I | CTNAG | 2 cut(s) | 160, 356 |
| KroI | GCCGGC | 1 cut(s) | 94 |
| KroNI | GCCGGC | 1 cut(s) | 96 |
| Kzo9I | GATC | 1 cut(s) | 141 |
| LmnI | GCTCC | 1 cut(s) | 223 |
| LpnPI | CCDG | 7 cut(s) | 47, 69, 108, 147, 234, 238, 309 |
| Lsp1109I | GCAGC | 1 cut(s) | 56 |
| MalI | GATC | 1 cut(s) | 143 |
| MbiI | CCGCTC | 1 cut(s) | 218 |
| MboI | GATC | 1 cut(s) | 141 |
| MluCI | AATT | 4 cut(s) | 167, 233, 318, 379 |
| MlyI | GAGTC | 2 cut(s) | 205, 299 |
| MmeI | TCCRAC | 1 cut(s) | 303 |
| MnlI | CCTC | 4 cut(s) | 9, 58, 156, 181 |
| MroNI | GCCGGC | 1 cut(s) | 94 |
| MspA1I | CMGCKG | 1 cut(s) | 299 |
| MspI | CCGG | 2 cut(s) | 95, 221 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 35, 215, 264 |
| NaeI | GCCGGC | 1 cut(s) | 96 |
| NdeII | GATC | 1 cut(s) | 141 |
| NgoMIV | GCCGGC | 1 cut(s) | 94 |
| NlaIV | GGNNCC | 1 cut(s) | 44 |
| NmeAIII | GCCGAG | 1 cut(s) | 48 |
| PdiI | GCCGGC | 1 cut(s) | 96 |
| PfeI | GAWTC | 1 cut(s) | 324 |
| PkrI | GCNGC | 2 cut(s) | 71, 93 |
| PleI | GAGTC | 2 cut(s) | 204, 298 |
| PpsI | GAGTC | 2 cut(s) | 204, 298 |
| PspFI | CCCAGC | 1 cut(s) | 295 |
| PspN4I | GGNNCC | 1 cut(s) | 44 |
| PspPI | GGNCC | 1 cut(s) | 42 |
| SatI | GCNGC | 2 cut(s) | 70, 92 |
| Sau3AI | GATC | 1 cut(s) | 141 |
| Sau96I | GGNCC | 1 cut(s) | 42 |
| SchI | GAGTC | 2 cut(s) | 205, 299 |
| SetI | ASST | 3 cut(s) | 71, 83, 166 |
| Sse9I | AATT | 4 cut(s) | 167, 233, 318, 379 |
| SsiI | CCGC | 5 cut(s) | 11, 92, 207, 216, 299 |
| TasI | AATT | 4 cut(s) | 167, 233, 318, 379 |
| TauI | GCSGC | 1 cut(s) | 94 |
| TfiI | GAWTC | 1 cut(s) | 324 |
| TseI | GCWGC | 1 cut(s) | 69 |
| TspDTI | ATGAA | 1 cut(s) | 410 |
| TspGWI | ACGGA | 2 cut(s) | 40, 295 |
| XapI | RAATTY | 2 cut(s) | 167, 233 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 317 |