Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 224 |
| AccI | GTMKAC | 1 cut(s) | 216 |
| AciI | CCGC | 3 cut(s) | 70, 222, 227 |
| AcsI | RAATTY | 3 cut(s) | 5, 173, 239 |
| AgsI | TTSAA | 1 cut(s) | 334 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 171, 203 |
| AluBI | AGCT | 2 cut(s) | 47, 94 |
| AluI | AGCT | 2 cut(s) | 47, 94 |
| Alw26I | GTCTC | 2 cut(s) | 141, 187 |
| AoxI | GGCC | 2 cut(s) | 66, 195 |
| ApeKI | GCWGC | 1 cut(s) | 94 |
| ApoI | RAATTY | 3 cut(s) | 5, 173, 239 |
| AspS9I | GGNCC | 1 cut(s) | 67 |
| AsuHPI | GGTGA | 1 cut(s) | 50 |
| BbvI | GCAGC | 1 cut(s) | 81 |
| BccI | CCATC | 5 cut(s) | 53, 84, 286, 324, 388 |
| BceAI | ACGGC | 2 cut(s) | 197, 241 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 240, 274 |
| BcoDI | GTCTC | 2 cut(s) | 141, 187 |
| BfaI | CTAG | 1 cut(s) | 362 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 60 |
| BisI | GCNGC | 1 cut(s) | 95 |
| BlsI | GCNGC | 1 cut(s) | 96 |
| BmgT120I | GGNCC | 1 cut(s) | 67 |
| BpmI | CTGGAG | 1 cut(s) | 212 |
| BseGI | GGATG | 1 cut(s) | 375 |
| BseMII | CTCAG | 3 cut(s) | 39, 167, 180 |
| BseXI | GCAGC | 1 cut(s) | 81 |
| BsgI | GTGCAG | 1 cut(s) | 80 |
| BshFI | GGCC | 2 cut(s) | 68, 197 |
| BsmAI | GTCTC | 2 cut(s) | 141, 187 |
| BsnI | GGCC | 2 cut(s) | 68, 197 |
| BspACI | CCGC | 3 cut(s) | 70, 222, 227 |
| BspANI | GGCC | 2 cut(s) | 68, 197 |
| BspCNI | CTCAG | 3 cut(s) | 40, 166, 179 |
| BspHI | TCATGA | 1 cut(s) | 9 |
| BsrBI | CCGCTC | 1 cut(s) | 224 |
| Bst4CI | ACNGT | 1 cut(s) | 307 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 270 |
| BstC8I | GCNNGC | 3 cut(s) | 70, 92, 118 |
| BstDEI | CTNAG | 4 cut(s) | 48, 153, 166, 274 |
| BstF5I | GGATG | 1 cut(s) | 375 |
| BstMAI | GTCTC | 2 cut(s) | 141, 187 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 60, 69, 270 |
| BstV1I | GCAGC | 1 cut(s) | 81 |
| BsuRI | GGCC | 2 cut(s) | 68, 197 |
| BtsCI | GGATG | 1 cut(s) | 375 |
| Cac8I | GCNNGC | 3 cut(s) | 70, 92, 118 |
| CciI | TCATGA | 1 cut(s) | 9 |
| Cfr13I | GGNCC | 1 cut(s) | 67 |
| CviAII | CATG | 1 cut(s) | 10 |
| CviJI | RGCY | 7 cut(s) | 47, 68, 94, 120, 197, 212, 256 |
| CviKI_1 | RGCY | 7 cut(s) | 47, 68, 94, 120, 197, 212, 256 |
| DdeI | CTNAG | 4 cut(s) | 48, 153, 166, 274 |
| EciI | GGCGGA | 1 cut(s) | 216 |
| FaeI | CATG | 1 cut(s) | 13 |
| FaiI | YATR | 5 cut(s) | 11, 19, 24, 81, 123 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 257, 289, 326, 358 |
| FatI | CATG | 1 cut(s) | 9 |
| FauI | CCCGC | 1 cut(s) | 77 |
| FblI | GTMKAC | 1 cut(s) | 216 |
| Fnu4HI | GCNGC | 1 cut(s) | 95 |
| FokI | GGATG | 1 cut(s) | 362 |
| Fsp4HI | GCNGC | 1 cut(s) | 95 |
| FspBI | CTAG | 1 cut(s) | 362 |
| GluI | GCNGC | 1 cut(s) | 95 |
| GsuI | CTGGAG | 1 cut(s) | 212 |
| HaeIII | GGCC | 2 cut(s) | 68, 197 |
| Hin1II | CATG | 1 cut(s) | 13 |
| HinfI | GANTC | 2 cut(s) | 296, 330 |
| HphI | GGTGA | 1 cut(s) | 50 |
| Hpy166II | GTNNAC | 1 cut(s) | 217 |
| Hpy188I | TCNGA | 6 cut(s) | 160, 169, 245, 286, 321, 385 |
| Hpy188III | TCNNGA | 3 cut(s) | 10, 109, 191 |
| Hpy8I | GTNNAC | 1 cut(s) | 217 |
| Hpy99I | CGWCG | 3 cut(s) | 262, 290, 307 |
| HpyAV | CCTTC | 1 cut(s) | 97 |
| HpyCH4III | ACNGT | 1 cut(s) | 307 |
| HpyCH4V | TGCA | 4 cut(s) | 97, 234, 390, 397 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 60, 69, 270 |
| HpyF3I | CTNAG | 4 cut(s) | 48, 153, 166, 274 |
| Hsp92II | CATG | 1 cut(s) | 13 |
| LmnI | GCTCC | 1 cut(s) | 229 |
| LpnPI | CCDG | 5 cut(s) | 72, 94, 130, 176, 244 |
| Lsp1109I | GCAGC | 1 cut(s) | 81 |
| MaeI | CTAG | 1 cut(s) | 362 |
| MbiI | CCGCTC | 1 cut(s) | 224 |
| MluCI | AATT | 4 cut(s) | 5, 173, 239, 324 |
| MlyI | GAGTC | 1 cut(s) | 305 |
| MmeI | TCCRAC | 1 cut(s) | 309 |
| MnlI | CCTC | 5 cut(s) | 34, 83, 162, 187, 310 |
| MseI | TTAA | 1 cut(s) | 351 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 60, 69, 270 |
| NlaIII | CATG | 1 cut(s) | 13 |
| PagI | TCATGA | 1 cut(s) | 9 |
| PfeI | GAWTC | 1 cut(s) | 330 |
| PkrI | GCNGC | 1 cut(s) | 96 |
| PleI | GAGTC | 1 cut(s) | 304 |
| PpsI | GAGTC | 1 cut(s) | 304 |
| PspPI | GGNCC | 1 cut(s) | 67 |
| SaqAI | TTAA | 1 cut(s) | 351 |
| SatI | GCNGC | 1 cut(s) | 95 |
| Sau96I | GGNCC | 1 cut(s) | 67 |
| SchI | GAGTC | 1 cut(s) | 305 |
| SetI | ASST | 3 cut(s) | 49, 96, 108 |
| Sse9I | AATT | 4 cut(s) | 5, 173, 239, 324 |
| SsiI | CCGC | 3 cut(s) | 70, 222, 227 |
| SspMI | CTAG | 1 cut(s) | 362 |
| TaaI | ACNGT | 1 cut(s) | 307 |
| TaqI | TCGA | 2 cut(s) | 260, 302 |
| TasI | AATT | 4 cut(s) | 5, 173, 239, 324 |
| TfiI | GAWTC | 1 cut(s) | 330 |
| Tru1I | TTAA | 1 cut(s) | 351 |
| Tru9I | TTAA | 1 cut(s) | 351 |
| TseI | GCWGC | 1 cut(s) | 94 |
| TspDTI | ATGAA | 3 cut(s) | 17, 26, 416 |
| XapI | RAATTY | 3 cut(s) | 5, 173, 239 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 83, 323 |
| XmiI | GTMKAC | 1 cut(s) | 216 |
| XspI | CTAG | 1 cut(s) | 362 |