Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 212 |
| AccI | GTMKAC | 1 cut(s) | 204 |
| AciI | CCGC | 4 cut(s) | 27, 61, 108, 210 |
| AcoI | YGGCCR | 1 cut(s) | 112 |
| AcsI | RAATTY | 3 cut(s) | 4, 161, 227 |
| AluBI | AGCT | 1 cut(s) | 85 |
| AluI | AGCT | 1 cut(s) | 85 |
| Alw26I | GTCTC | 1 cut(s) | 175 |
| AoxI | GGCC | 3 cut(s) | 57, 112, 183 |
| ApeKI | GCWGC | 1 cut(s) | 85 |
| ApoI | RAATTY | 3 cut(s) | 4, 161, 227 |
| AspS9I | GGNCC | 1 cut(s) | 58 |
| AsuHPI | GGTGA | 1 cut(s) | 41 |
| BbvI | GCAGC | 1 cut(s) | 72 |
| BccI | CCATC | 1 cut(s) | 262 |
| BcoDI | GTCTC | 1 cut(s) | 175 |
| BglII | AGATCT | 1 cut(s) | 175 |
| BisI | GCNGC | 2 cut(s) | 86, 108 |
| BlsI | GCNGC | 2 cut(s) | 87, 109 |
| BmgT120I | GGNCC | 1 cut(s) | 58 |
| BpmI | CTGGAG | 2 cut(s) | 198, 200 |
| Bse118I | RCCGGY | 1 cut(s) | 110 |
| BseMII | CTCAG | 2 cut(s) | 168, 186 |
| BseXI | GCAGC | 1 cut(s) | 72 |
| BsgI | GTGCAG | 1 cut(s) | 71 |
| BshFI | GGCC | 3 cut(s) | 59, 114, 185 |
| BsiSI | CCGG | 2 cut(s) | 78, 111 |
| BsmAI | GTCTC | 1 cut(s) | 175 |
| BsnI | GGCC | 3 cut(s) | 59, 114, 185 |
| Bsp143I | GATC | 1 cut(s) | 175 |
| BspACI | CCGC | 4 cut(s) | 27, 61, 108, 210 |
| BspANI | GGCC | 3 cut(s) | 59, 114, 185 |
| BspCNI | CTCAG | 2 cut(s) | 167, 185 |
| BsrBI | CCGCTC | 1 cut(s) | 212 |
| BsrFI | RCCGGY | 1 cut(s) | 110 |
| BssAI | RCCGGY | 1 cut(s) | 110 |
| BssMI | GATC | 1 cut(s) | 175 |
| Bst4CI | ACNGT | 1 cut(s) | 202 |
| BstC8I | GCNNGC | 4 cut(s) | 61, 83, 112, 220 |
| BstDEI | CTNAG | 2 cut(s) | 154, 172 |
| BstKTI | GATC | 1 cut(s) | 178 |
| BstMAI | GTCTC | 1 cut(s) | 175 |
| BstMBI | GATC | 1 cut(s) | 175 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 51, 60 |
| BstV1I | GCAGC | 1 cut(s) | 72 |
| BstX2I | RGATCY | 1 cut(s) | 175 |
| BstYI | RGATCY | 1 cut(s) | 175 |
| BsuRI | GGCC | 3 cut(s) | 59, 114, 185 |
| Cac8I | GCNNGC | 4 cut(s) | 61, 83, 112, 220 |
| Cfr10I | RCCGGY | 1 cut(s) | 110 |
| Cfr13I | GGNCC | 1 cut(s) | 58 |
| CviAII | CATG | 1 cut(s) | 55 |
| CviJI | RGCY | 6 cut(s) | 38, 59, 85, 114, 185, 218 |
| CviKI_1 | RGCY | 6 cut(s) | 38, 59, 85, 114, 185, 218 |
| DdeI | CTNAG | 2 cut(s) | 154, 172 |
| DpnI | GATC | 1 cut(s) | 177 |
| DpnII | GATC | 1 cut(s) | 175 |
| EaeI | YGGCCR | 1 cut(s) | 112 |
| FaeI | CATG | 1 cut(s) | 58 |
| FaiI | YATR | 4 cut(s) | 10, 56, 72, 117 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 233, 265 |
| FatI | CATG | 1 cut(s) | 54 |
| FauI | CCCGC | 1 cut(s) | 68 |
| FblI | GTMKAC | 1 cut(s) | 204 |
| Fnu4HI | GCNGC | 2 cut(s) | 86, 108 |
| Fsp4HI | GCNGC | 2 cut(s) | 86, 108 |
| GluI | GCNGC | 2 cut(s) | 86, 108 |
| GsuI | CTGGAG | 2 cut(s) | 198, 200 |
| HaeIII | GGCC | 3 cut(s) | 59, 114, 185 |
| HapII | CCGG | 2 cut(s) | 78, 111 |
| Hin1II | CATG | 1 cut(s) | 58 |
| HinfI | GANTC | 2 cut(s) | 190, 272 |
| HpaII | CCGG | 2 cut(s) | 78, 111 |
| HphI | GGTGA | 1 cut(s) | 41 |
| Hpy166II | GTNNAC | 1 cut(s) | 205 |
| Hpy188I | TCNGA | 4 cut(s) | 148, 175, 233, 262 |
| Hpy188III | TCNNGA | 3 cut(s) | 100, 179, 194 |
| Hpy8I | GTNNAC | 1 cut(s) | 205 |
| Hpy99I | CGWCG | 1 cut(s) | 266 |
| HpyAV | CCTTC | 1 cut(s) | 88 |
| HpyCH4III | ACNGT | 1 cut(s) | 202 |
| HpyCH4V | TGCA | 2 cut(s) | 88, 222 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 51, 60 |
| HpyF3I | CTNAG | 2 cut(s) | 154, 172 |
| Hsp92II | CATG | 1 cut(s) | 58 |
| KroI | GCCGGC | 1 cut(s) | 110 |
| KroNI | GCCGGC | 1 cut(s) | 112 |
| Kzo9I | GATC | 1 cut(s) | 175 |
| LmnI | GCTCC | 1 cut(s) | 217 |
| LpnPI | CCDG | 7 cut(s) | 85, 91, 124, 141, 164, 228, 232 |
| Lsp1109I | GCAGC | 1 cut(s) | 72 |
| MalI | GATC | 1 cut(s) | 177 |
| MbiI | CCGCTC | 1 cut(s) | 212 |
| MboI | GATC | 1 cut(s) | 175 |
| MflI | RGATCY | 1 cut(s) | 175 |
| MluCI | AATT | 3 cut(s) | 4, 161, 227 |
| MlyI | GAGTC | 2 cut(s) | 199, 281 |
| MnlI | CCTC | 3 cut(s) | 25, 74, 175 |
| MroNI | GCCGGC | 1 cut(s) | 110 |
| MspI | CCGG | 2 cut(s) | 78, 111 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 51, 60 |
| NaeI | GCCGGC | 1 cut(s) | 112 |
| NdeII | GATC | 1 cut(s) | 175 |
| NgoMIV | GCCGGC | 1 cut(s) | 110 |
| NlaIII | CATG | 1 cut(s) | 58 |
| NmeAIII | GCCGAG | 1 cut(s) | 64 |
| PdiI | GCCGGC | 1 cut(s) | 112 |
| PkrI | GCNGC | 2 cut(s) | 87, 109 |
| PleI | GAGTC | 2 cut(s) | 198, 280 |
| PpsI | GAGTC | 2 cut(s) | 198, 280 |
| PspPI | GGNCC | 1 cut(s) | 58 |
| PsuI | RGATCY | 1 cut(s) | 175 |
| SatI | GCNGC | 2 cut(s) | 86, 108 |
| Sau3AI | GATC | 1 cut(s) | 175 |
| Sau96I | GGNCC | 1 cut(s) | 58 |
| SchI | GAGTC | 2 cut(s) | 199, 281 |
| SetI | ASST | 3 cut(s) | 87, 99, 160 |
| Sse9I | AATT | 3 cut(s) | 4, 161, 227 |
| SsiI | CCGC | 4 cut(s) | 27, 61, 108, 210 |
| TaaI | ACNGT | 1 cut(s) | 202 |
| TasI | AATT | 3 cut(s) | 4, 161, 227 |
| TauI | GCSGC | 1 cut(s) | 110 |
| TseI | GCWGC | 1 cut(s) | 85 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 56 |
| XapI | RAATTY | 3 cut(s) | 4, 161, 227 |
| XmiI | GTMKAC | 1 cut(s) | 204 |