Rmu_sc0001767.1_g000005

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001767.1
OG0005383
Physical Location & Seq
Reverse (-)
16714 .. 25764
9051 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2712 bp
atggtggtgcgcggaggtgtcaattaccctgcagtggagttggttgatttcactaagtgggagtttgatccggtgggtggtgtcgagatctattttgttctcgatgcaactcatggttcccaacgacaacgaattcgtggtaccgatcggcagtctctgctcaataccattcggttttgccctaaatttcagacaacaaaacgctgccatcgcattcaatacatttgccgaacaagtgatctacatgcaagtagagcacaaagtttgctgatggcttatcagaataatattaatccaaatctatctgaagatgacgaggaacctttagttttcaaacggtctgcagcatctaaacaaaatcagtcaaacacagacacaaagaaattatcgtctcagaggtgtgatggacaaccacgaagagtctctgacgtacactcttccaatggtgtaaactctagtgttcaaaagggtaagatggctccatctacaaaggcatcaccagggaaatctcccacagcgatcccaaaagagttatcagaacaaaacaaggcgatctctattaaagaggaaaatattcctattgaacaccctgctgaagacaacagtgattctgacgatgaaaaaccgataaattccagattgaaggctaaaataaacaatgtcaaaaaggaagattcagatgatgaagatgataaacccttatcgacaagggttaataagtccaatgtgggaacctcaggctgtaagccaggtgacgctaaagaaaggaagccattggttcagaagaatggttcaagcacaatagataaagggaaaagctcttctttggtgtcaggtaagaggcctctagataaggcgaatcatgctgagcagtcttcaattaaaaagccaaaaattgcagagtcttcaacatctataaagagtaagcaggtgtcggccaaaacagagctgaaggcagatgatgatgacatgacactttcccagagaatgaagaaagccacatctgctaataaatcatctctgacgaagaaaactgtaactaaagtcagttcatcctcaatccagaaaaagatccagaaaggcaaaaaaacagataagttttcaaagtattctaagtcaacaagacaaggacctagctctaatgatgggcagaaaaaatggactactttagagcacaatggtgtcattttcccacctccttacaagcctcacggggtaaagatgctttacaatggcaagccagtgaacttgactcctgaacaagaggaggttgcaacaatgtatgcagtgatgatagatacagaatatgtgcagaaaaaaactttcagagacaacttctggaatgattggcgtaagttgcttgggaagaaccatgtaattcagaaattggatgcttgtgatttcaaaccaatatacgactggtatcagaaagaaaaggagaagaagaaacaaatgagtacagaagagaagaaagctgtcaaggaagagaaattaaaacaagaggagaagtatatgtgggctgttgttgatggtgtaaaagagaaggttggaaacttcagagttgaaccacctggattattccgaggccgtggcgagcatccgaagtcaggaaagctgaagaggcggattagtccaagtgaagttacaataaatataggaaaggatgccgaggttcccgaatgccccattcctggtgaaaggtggaaagaagtaaggcatgacaatactgttacatggttggctttctggaatgatccaatcaatccaaaggagttcaaatatgtatttcttgcagctagtagtgccttgaaggggcaaagcgacaaggagaaatatgagaaagcaaggaagttgaaggactacataggagatattagggctgcatacactagggattttacgagtagagatcctgcaaagcggcaaatagctgttgctacatatctcattgacaaacttgctctgagggctggaaatgagaaggatgatgatgaagctgatactgttggttgctgcacattaaaagtagagaacgtaaaagcaattccccccaactcattggagtttaacttccttgggaaaaaggacagtgatgatctctttgacaagttagataccagtaaattgaatgcacatctaaaggaactgatgcctggtcttactgcaaaagtgttccgtacatacaatgcatctatcacattggataatgaattgtaccaggaaactgcagagggagatgtattgaagaaaaaagttttttatgatacagcaaacaagaaggttgctattatctgtaatcatcagcgggctgtgtcaaagtctcatgaatcccaaatggacaagctaaaggaaaagcttagtgaccttcagagtgttttaaaagagctaaagacagatttggacagggcaagaaatggaaagcccccactgaaagatgctgatggaaagcagaagagaaatttgaccccggaagcgttacagaagaagatagctgatgccaataaaaagattgataaagtaaaactggatatgcaaaccaaagacgacatgaaaacaattgcattgggaacatcaaaaatcaactatcttgatcctagaatcaccgttgcatggtgcaaacgacatgaagtccccattgagaagatattccagaaggctctcctggcaaagtttgcttgggcaatggatgttgatcctgacttcagattctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

903

Amino Acids

102.11

Weight (kDa)

9.44

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 385 - 597 4.2e-95 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 600 - 695 5.3e-40 Eukaryotic DNA topoisomerase I, catalytic core
Topoisom_I PF01028 694 - 799 2.3e-37 Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc PF14370 834 - 903 5.8e-35 C-terminal topoisomerase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 919
Acc36I ACCTGC 1 cut(s) 919
Acc65I GGTACC 1 cut(s) 140
AccB1I GGYRCC 1 cut(s) 140
AccII CGCG 1 cut(s) 12
AciI CCGC 4 cut(s) 12, 1624, 1921, 2305
AclWI GGATC 7 cut(s) 62, 514, 1066, 1748, 1904, 2585, 2687
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 4 cut(s) 132, 185, 631, 2458
AcuI CTGAAG 7 cut(s) 327, 615, 971, 1540, 1637, 2351, 2686
AdeI CACNNNGTG 1 cut(s) 57
AfaI GTAC 5 cut(s) 142, 432, 1459, 2179, 2216
AfiI CCNNNNNNNGG 6 cut(s) 34, 77, 1607, 1691, 1812, 2610
AhdI GACNNNNNGTC 1 cut(s) 2627
AjnI CCWGG 7 cut(s) 499, 748, 1570, 1690, 2152, 2217, 2661
AleI CACNNNNGTG 1 cut(s) 1179
Alw21I GWGCWC 2 cut(s) 259, 1176
Alw26I GTCTC 5 cut(s) 159, 396, 427, 1323, 2325
AlwI GGATC 7 cut(s) 62, 514, 1066, 1748, 1904, 2585, 2687
AlwNI CAGNNNCTG 2 cut(s) 157, 2709
AoxI GGCC 3 cut(s) 842, 936, 1585
ApeKI GCWGC 5 cut(s) 204, 344, 1793, 1880, 2013
ApoI RAATTY 4 cut(s) 132, 185, 631, 2458
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
AseI ATTAAT 1 cut(s) 291
Asp700I GAANNNNTTC 2 cut(s) 573, 2645
Asp718I GGTACC 1 cut(s) 140
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 1 cut(s) 1130
AsuC2I CCSGG 1 cut(s) 2468
AsuHPI GGTGA 4 cut(s) 489, 764, 1706, 2593
AvaII GGWCC 1 cut(s) 1130
AxyI CCTNAGG 1 cut(s) 736
BanI GGYRCC 1 cut(s) 140
BbsI GAAGAC 3 cut(s) 603, 867, 897
Bbv12I GWGCWC 2 cut(s) 259, 1176
BbvI GCAGC 5 cut(s) 191, 356, 1805, 1867, 2000
BccI CCATC 8 cut(s) 216, 265, 398, 469, 490, 1139, 1523, 2435
BceAI ACGGC 1 cut(s) 1572
BciT130I CCWGG 7 cut(s) 501, 750, 1572, 1692, 2154, 2219, 2663
BcnI CCSGG 1 cut(s) 2468
BcoDI GTCTC 5 cut(s) 159, 396, 427, 1323, 2325
BfaI CTAG 6 cut(s) 456, 848, 1134, 1797, 1890, 2595
BfmI CTRYAG 3 cut(s) 30, 342, 2226
BfuAI ACCTGC 1 cut(s) 919
BglII AGATCT 1 cut(s) 87
BisI GCNGC 6 cut(s) 205, 345, 1794, 1881, 1922, 2014
BlpI GCTNAGC 1 cut(s) 867
BlsI GCNGC 6 cut(s) 206, 346, 1795, 1882, 1923, 2015
Bme1390I CCNGG 8 cut(s) 501, 750, 1572, 1692, 2154, 2219, 2468, 2663
Bme18I GGWCC 1 cut(s) 1130
BmeRI GACNNNNNGTC 1 cut(s) 2627
BmgT120I GGNCC 1 cut(s) 1130
BmiI GGNNCC 6 cut(s) 118, 142, 321, 480, 733, 1674
BmrFI CCNGG 8 cut(s) 501, 750, 1572, 1692, 2154, 2219, 2468, 2663
BpiI GAAGAC 3 cut(s) 603, 867, 897
Bpu1102I GCTNAGC 1 cut(s) 867
BpuMI CCSGG 1 cut(s) 2468
BsaBI GATNNNNATC 1 cut(s) 2691
BsaJI CCNNGG 6 cut(s) 500, 1582, 1588, 1668, 2074, 2466
BsaWI WCCGGW 1 cut(s) 70
Bsc4I CCNNNNNNNGG 6 cut(s) 34, 77, 1607, 1691, 1812, 2610
Bse1I ACTGG 4 cut(s) 1241, 1424, 2118, 2529
Bse21I CCTNAGG 1 cut(s) 736
Bse3DI GCAATG 1 cut(s) 2688
Bse8I GATNNNNATC 1 cut(s) 2691
BseBI CCWGG 7 cut(s) 501, 750, 1572, 1692, 2154, 2219, 2663
BseDI CCNNGG 6 cut(s) 500, 1582, 1588, 1668, 2074, 2466
BseGI GGATG 6 cut(s) 1052, 1396, 1597, 1669, 1990, 2692
BseJI GATNNNNATC 1 cut(s) 2691
BseLI CCNNNNNNNGG 6 cut(s) 34, 77, 1607, 1691, 1812, 2610
BseMI GCAATG 1 cut(s) 2688
BseMII CTCAG 4 cut(s) 407, 750, 858, 1955
BseNI ACTGG 4 cut(s) 1241, 1424, 2118, 2529
BseRI GAGGAG 2 cut(s) 1280, 1517
BseXI GCAGC 5 cut(s) 191, 356, 1805, 1867, 2000
BsgI GTGCAG 2 cut(s) 1331, 1999
Bsh1236I CGCG 1 cut(s) 12
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 3 cut(s) 844, 938, 1587
BshNI GGYRCC 1 cut(s) 140
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 2 cut(s) 259, 1176
BsiSI CCGG 2 cut(s) 71, 2468
BslFI GGGAC 1 cut(s) 2615
BslI CCNNNNNNNGG 6 cut(s) 34, 77, 1607, 1691, 1812, 2610
BsmAI GTCTC 5 cut(s) 159, 396, 427, 1323, 2325
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 1 cut(s) 2615
BsmI GAATGC 3 cut(s) 213, 1685, 2134
BsnI GGCC 3 cut(s) 844, 938, 1587
Bsp1286I GDGCHC 2 cut(s) 259, 1176
Bsp1720I GCTNAGC 1 cut(s) 867
BspACI CCGC 4 cut(s) 12, 1624, 1921, 2305
BspANI GGCC 3 cut(s) 844, 938, 1587
BspCNI CTCAG 4 cut(s) 406, 749, 859, 1956
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 1 cut(s) 2323
BspLI GGNNCC 6 cut(s) 118, 142, 321, 480, 733, 1674
BspMAI CTGCAG 3 cut(s) 34, 346, 2230
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 7 cut(s) 62, 514, 1066, 1748, 1904, 2585, 2687
BspQI GCTCTTC 1 cut(s) 826
BspT107I GGYRCC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 2688
BsrI ACTGG 4 cut(s) 1241, 1424, 2118, 2529
BssECI CCNNGG 6 cut(s) 500, 1582, 1588, 1668, 2074, 2466
BssT1I CCWWGG 1 cut(s) 2074
Bst2UI CCWGG 7 cut(s) 501, 750, 1572, 1692, 2154, 2219, 2663
Bst4CI ACNGT 7 cut(s) 339, 605, 1036, 1729, 2005, 2090, 2605
Bst6I CTCTTC 7 cut(s) 412, 442, 826, 1458, 1479, 1613, 2447
BstAPI GCANNNNNTGC 2 cut(s) 157, 2672
BstC8I GCNNGC 3 cut(s) 1238, 1595, 2307
BstDEI CTNAG 7 cut(s) 54, 393, 736, 867, 1113, 1964, 2357
BstDSI CCRYGG 1 cut(s) 1588
BstF5I GGATG 6 cut(s) 1052, 1396, 1597, 1669, 1990, 2692
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 12
BstMAI GTCTC 5 cut(s) 159, 396, 427, 1323, 2325
BstMCI CGRYCG 1 cut(s) 148
BstNI CCWGG 7 cut(s) 501, 750, 1572, 1692, 2154, 2219, 2663
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 8 cut(s) 499, 748, 1570, 1690, 2152, 2217, 2466, 2661
BstSFI CTRYAG 3 cut(s) 30, 342, 2226
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 5 cut(s) 191, 356, 1805, 1867, 2000
BstV2I GAAGAC 3 cut(s) 603, 867, 897
BstX2I RGATCY 3 cut(s) 87, 1071, 1909
BstXI CCANNNNNNTGG 1 cut(s) 2059
BstYI RGATCY 3 cut(s) 87, 1071, 1909
Bsu36I CCTNAGG 1 cut(s) 736
BsuRI GGCC 3 cut(s) 844, 938, 1587
BtgI CCRYGG 1 cut(s) 1588
BtgZI GCGATG 1 cut(s) 194
BtsCI GGATG 6 cut(s) 1052, 1396, 1597, 1669, 1990, 2692
BtsI GCAGTG 2 cut(s) 39, 1293
BtsIMutI CAGTG 6 cut(s) 39, 610, 1248, 1293, 2095, 2426
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 3 cut(s) 1238, 1595, 2307
CaiI CAGNNNCTG 2 cut(s) 157, 2709
CciI TCATGA 1 cut(s) 2323
CfoI GCGC 1 cut(s) 12
Cfr13I GGNCC 1 cut(s) 1130
CseI GACGC 1 cut(s) 764
Csp6I GTAC 5 cut(s) 141, 431, 1458, 2178, 2215
CviQI GTAC 5 cut(s) 141, 431, 1458, 2178, 2215
DdeI CTNAG 7 cut(s) 54, 393, 736, 867, 1113, 1964, 2357
DraI TTTAAA 1 cut(s) 2379
DraIII CACNNNGTG 1 cut(s) 57
DriI GACNNNNNGTC 1 cut(s) 2627
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 7 cut(s) 412, 442, 826, 1458, 1479, 1613, 2447
Eam1105I GACNNNNNGTC 1 cut(s) 2627
EarI CTCTTC 7 cut(s) 412, 442, 826, 1458, 1479, 1613, 2447
EciI GGCGGA 1 cut(s) 1639
Eco130I CCWWGG 1 cut(s) 2074
Eco147I AGGCCT 1 cut(s) 844
Eco47I GGWCC 1 cut(s) 1130
Eco57I CTGAAG 7 cut(s) 327, 615, 971, 1540, 1637, 2351, 2686
Eco81I CCTNAGG 1 cut(s) 736
EcoO109I RGGNCCY 1 cut(s) 1130
EcoRI GAATTC 1 cut(s) 132
EcoRII CCWGG 7 cut(s) 499, 748, 1570, 1690, 2152, 2217, 2661
EcoT14I CCWWGG 1 cut(s) 2074
EcoT22I ATGCAT 1 cut(s) 2191
ErhI CCWWGG 1 cut(s) 2074
Esp3I CGTCTC 1 cut(s) 396
FalI AAGNNNNNCTT 2 cut(s) 808, 840
FaqI GGGAC 1 cut(s) 2615
FauI CCCGC 1 cut(s) 2298
Fnu4HI GCNGC 6 cut(s) 205, 345, 1794, 1881, 1922, 2014
FokI GGATG 6 cut(s) 1039, 1403, 1584, 1676, 1997, 2699
Fsp4HI GCNGC 6 cut(s) 205, 345, 1794, 1881, 1922, 2014
FspBI CTAG 6 cut(s) 456, 848, 1134, 1797, 1890, 2595
GlaI GCGC 1 cut(s) 11
GluI GCNGC 6 cut(s) 205, 345, 1794, 1881, 1922, 2014
HaeIII GGCC 3 cut(s) 844, 938, 1587
HapII CCGG 2 cut(s) 71, 2468
HgaI GACGC 1 cut(s) 764
HhaI GCGC 1 cut(s) 12
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HincII GTYRAC 1 cut(s) 1119
HindII GTYRAC 1 cut(s) 1119
HindIII AAGCTT 1 cut(s) 2354
HinfI GANTC 9 cut(s) 420, 608, 674, 859, 902, 1252, 2327, 2598, 2706
HpaII CCGG 2 cut(s) 71, 2468
HphI GGTGA 4 cut(s) 489, 764, 1706, 2593
Hpy166II GTNNAC 4 cut(s) 433, 451, 1119, 1246
Hpy8I GTNNAC 4 cut(s) 433, 451, 1119, 1246
HpyAV CCTTC 9 cut(s) 637, 946, 1537, 1804, 1849, 1975, 2272, 2375, 2647
HpyCH4III ACNGT 7 cut(s) 339, 605, 1036, 1729, 2005, 2090, 2605
HpyCH4IV ACGT 2 cut(s) 429, 2034
HpyF3I CTNAG 7 cut(s) 54, 393, 736, 867, 1113, 1964, 2357
HpySE526I ACGT 2 cut(s) 429, 2034
HspAI GCGC 1 cut(s) 10
KpnI GGTACC 1 cut(s) 144
LguI GCTCTTC 1 cut(s) 826
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 5 cut(s) 191, 356, 1805, 1867, 2000
MaeI CTAG 6 cut(s) 456, 848, 1134, 1797, 1890, 2595
MaeII ACGT 2 cut(s) 429, 2034
MaeIII GTNAC 6 cut(s) 752, 1036, 1642, 1729, 2360, 2475
MfeI CAATTG 1 cut(s) 2556
MflI RGATCY 3 cut(s) 87, 1071, 1909
MhlI GDGCHC 2 cut(s) 259, 1176
MlyI GAGTC 3 cut(s) 429, 911, 1246
MmeI TCCRAC 1 cut(s) 1528
Mph1103I ATGCAT 1 cut(s) 2191
MroXI GAANNNNTTC 2 cut(s) 573, 2645
MseI TTAA 8 cut(s) 291, 561, 714, 882, 1493, 2021, 2067, 2378
MslI CAYNNNNRTG 1 cut(s) 1179
MspA1I CMGCKG 1 cut(s) 2305
MspI CCGG 2 cut(s) 71, 2468
MspR9I CCNGG 8 cut(s) 501, 750, 1572, 1692, 2154, 2219, 2468, 2663
MunI CAATTG 1 cut(s) 2556
Mva1269I GAATGC 3 cut(s) 213, 1685, 2134
MvaI CCWGG 7 cut(s) 501, 750, 1572, 1692, 2154, 2219, 2663
MvnI CGCG 1 cut(s) 12
NciI CCSGG 1 cut(s) 2468
NlaIV GGNNCC 6 cut(s) 118, 142, 321, 480, 733, 1674
NmeAIII GCCGAG 1 cut(s) 1693
NmuCI GTSAC 2 cut(s) 752, 2360
NsiI ATGCAT 1 cut(s) 2191
NspI RCATGY 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 1179
PagI TCATGA 1 cut(s) 2323
PaqCI CACCTGC 1 cut(s) 919
PceI AGGCCT 1 cut(s) 844
PciSI GCTCTTC 1 cut(s) 826
PctI GAATGC 3 cut(s) 213, 1685, 2134
PdmI GAANNNNTTC 2 cut(s) 573, 2645
PfeI GAWTC 6 cut(s) 608, 674, 859, 2327, 2598, 2706
PkrI GCNGC 6 cut(s) 206, 346, 1795, 1882, 1923, 2015
Ple19I CGATCG 1 cut(s) 148
PleI GAGTC 3 cut(s) 428, 910, 1246
PpsI GAGTC 3 cut(s) 428, 910, 1246
PpuMI RGGWCCY 1 cut(s) 1130
PshBI ATTAAT 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 1130
Psp6I CCWGG 7 cut(s) 499, 748, 1570, 1690, 2152, 2217, 2661
PspGI CCWGG 7 cut(s) 499, 748, 1570, 1690, 2152, 2217, 2661
PspN4I GGNNCC 6 cut(s) 118, 142, 321, 480, 733, 1674
PspPI GGNCC 1 cut(s) 1130
PspPPI RGGWCCY 1 cut(s) 1130
PstI CTGCAG 3 cut(s) 34, 346, 2230
PstNI CAGNNNCTG 2 cut(s) 157, 2709
PsuI RGATCY 3 cut(s) 87, 1071, 1909
PvuI CGATCG 1 cut(s) 148
RsaI GTAC 5 cut(s) 142, 432, 1459, 2179, 2216
RsaNI GTAC 5 cut(s) 141, 431, 1458, 2178, 2215
RseI CAYNNNNRTG 1 cut(s) 1179
SapI GCTCTTC 1 cut(s) 826
SaqAI TTAA 8 cut(s) 291, 561, 714, 882, 1493, 2021, 2067, 2378
SatI GCNGC 6 cut(s) 205, 345, 1794, 1881, 1922, 2014
Sau96I GGNCC 1 cut(s) 1130
SchI GAGTC 3 cut(s) 429, 911, 1246
ScrFI CCNGG 8 cut(s) 501, 750, 1572, 1692, 2154, 2219, 2468, 2663
SduI GDGCHC 2 cut(s) 259, 1176
SfcI CTRYAG 3 cut(s) 30, 342, 2226
SinI GGWCC 1 cut(s) 1130
SmiMI CAYNNNNRTG 1 cut(s) 1179
SseBI AGGCCT 1 cut(s) 844
SsiI CCGC 4 cut(s) 12, 1624, 1921, 2305
SspI AATATT 2 cut(s) 289, 574
SspMI CTAG 6 cut(s) 456, 848, 1134, 1797, 1890, 2595
StuI AGGCCT 1 cut(s) 844
StyD4I CCNGG 8 cut(s) 499, 748, 1570, 1690, 2152, 2217, 2466, 2661
StyI CCWWGG 1 cut(s) 2074
TaaI ACNGT 7 cut(s) 339, 605, 1036, 1729, 2005, 2090, 2605
TaiI ACGT 2 cut(s) 432, 2037
TaqI TCGA 3 cut(s) 84, 102, 704
TatI WGTACW 1 cut(s) 1457
TauI GCSGC 1 cut(s) 1924
TfiI GAWTC 6 cut(s) 608, 674, 859, 2327, 2598, 2706
Tru1I TTAA 8 cut(s) 291, 561, 714, 882, 1493, 2021, 2067, 2378
Tru9I TTAA 8 cut(s) 291, 561, 714, 882, 1493, 2021, 2067, 2378
TscAI CASTG 6 cut(s) 39, 610, 1248, 1293, 2095, 2433
TseFI GTSAC 2 cut(s) 752, 2360
TseI GCWGC 5 cut(s) 204, 344, 1793, 1880, 2013
Tsp45I GTSAC 2 cut(s) 752, 2360
TspDTI ATGAA 9 cut(s) 633, 699, 1004, 1041, 2007, 2223, 2340, 2564, 2640
TspGWI ACGGA 1 cut(s) 2165
TspRI CASTG 6 cut(s) 39, 610, 1248, 1293, 2095, 2433
VpaK11BI GGWCC 1 cut(s) 1130
VspI ATTAAT 1 cut(s) 291
XapI RAATTY 4 cut(s) 132, 185, 631, 2458
XbaI TCTAGA 1 cut(s) 847
XceI RCATGY 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 1416
XmnI GAANNNNTTC 2 cut(s) 573, 2645
XspI CTAG 6 cut(s) 456, 848, 1134, 1797, 1890, 2595
Zsp2I ATGCAT 1 cut(s) 2191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.