Rmu_sc0001884.1_g000033

REF SRPP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001884.1
OG0002647
Physical Location & Seq
Reverse (-)
129767 .. 134303
4537 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1521 bp
atggcggaggaggtggaagagcagcagcggctcaagtacctgcaatttgttcaagtggctgcacttcacgccgcggtttgtttctcaaatgcttatggttatgccaaggagaaatccggtccactgaggcccggcgttgagtcagcggagggagctgtgaagactgttgttggacctgtgtatgacacgtaccaggctctccccgccaacctcctggctttcgttgatggcaaggtcgacgaatctgtgaccaggctggcttcatcccaagcaatgtcttttgctcagaaagcgccagaggcggctcgagctgctgcatcggagtttcagcaaaggagcacaagcatggctaaatcggtatactccaagtacgaaccgaaagctgagcaatgtgctgtttgtgcatggagaaagctgaatgagctacctgtggtaccgcaggtggctgagattgttgtaccgactgctgcatattgttccgaaaagtacaaccacagtgtccgttgcactgcggagaaggggtacagggtggcctcctatttgcctttggttccaacagaaaaaattgctaaggtttttggagggaatgtgcctgaacccgttcgcccaaatactacttcgggtagaaaacacaggttcaattatggagctgctcaatgccctttcaaattgaatgaaactgacccgagctcctcggtgatgacagcagcgttgccattcggcgatcatcaacaatatatcatcgagcaaattccagtccggtctaagtctccgccggaccgtacaccgctaggagacaccgtaggcaaaacctccgccagcaacaaatttctggccaaaacctccaccactagccggacaacctccactgcttggacaaacctccactccactacccaaacttctgctacccatactaagcccaaacagatgtggacaaagaaaccccgagcttcctctcacgccttcgctgcaaccacgagctctaacgctcatgccttcgcggcgaccccgagattcttctcacgtctttgtggcaccccgaccttctcgctcaggccttcgcggcagcccgagctttccgctcacgccttcgcggcaacctggagcttcccgctcaagagcttccactcacgtcttcgccacaacctcgagcttccagctcacgagcttccgctcacgcctttgaggcacccccgagcttccgctcacaccttggcaacaacccccgagctttctctcacgccttcccggcaacccccgagctcccactcaggccttcctggcaacccccgagcttctcgctcacgtcttcctggcaaccctcgagcttccctctcacgccttcccgacaacccggagcttccgcccaggccttcccgacaaccctcgagctttccgctcacgccttcccggcaacccttgagctacccgctcataccttcccggcacccccagctttcacgctcttaccttccggcattccccagctttacactcacatcccctcgacatcattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
REF

Protein Analysis

506

Amino Acids

55.37

Weight (kDa)

9.97

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 6 - 194 1.3e-72 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 430
Acc36I ACCTGC 2 cut(s) 48, 430
Acc65I GGTACC 1 cut(s) 433
AccB1I GGYRCC 4 cut(s) 433, 1037, 1191, 1449
AccB7I CCANNNNNTGG 1 cut(s) 873
AccBSI CCGCTC 6 cut(s) 1085, 1117, 1177, 1208, 1402, 1435
AccI GTMKAC 2 cut(s) 237, 360
AccII CGCG 4 cut(s) 74, 1004, 1066, 1097
AcoI YGGCCR 1 cut(s) 834
AcsI RAATTY 2 cut(s) 750, 827
AfaI GTAC 8 cut(s) 38, 191, 371, 435, 459, 488, 524, 784
AfiI CCNNNNNNNGG 2 cut(s) 855, 873
AflIII ACRYGT 1 cut(s) 186
AgsI TTSAA 4 cut(s) 53, 640, 667, 673
AjiI CACGTC 3 cut(s) 1028, 1136, 1310
AjnI CCWGG 7 cut(s) 192, 213, 251, 1103, 1282, 1315, 1370
AjuI GAANNNNNNNTTGG 2 cut(s) 601, 633
Alw21I GWGCWC 4 cut(s) 341, 692, 986, 1268
Alw26I GTCTC 2 cut(s) 774, 789
AoxI GGCC 6 cut(s) 128, 531, 834, 1058, 1276, 1373
ApoI RAATTY 2 cut(s) 750, 827
Asp700I GAANNNNTTC 1 cut(s) 600
Asp718I GGTACC 1 cut(s) 433
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 4 cut(s) 119, 129, 173, 778
AsuC2I CCSGG 5 cut(s) 132, 1252, 1358, 1414, 1447
AsuHPI GGTGA 1 cut(s) 709
AvaII GGWCC 3 cut(s) 119, 173, 778
BalI TGGCCA 1 cut(s) 836
BanI GGYRCC 4 cut(s) 433, 1037, 1191, 1449
BanII GRGCYC 3 cut(s) 692, 986, 1268
BauI CACGAG 2 cut(s) 979, 1166
BbsI GAAGAC 3 cut(s) 167, 1130, 1304
Bbv12I GWGCWC 4 cut(s) 341, 692, 986, 1268
BccI CCATC 1 cut(s) 221
BciT130I CCWGG 7 cut(s) 194, 215, 253, 1105, 1284, 1317, 1372
BcnI CCSGG 5 cut(s) 132, 1252, 1358, 1414, 1447
BcoDI GTCTC 2 cut(s) 774, 789
BfaI CTAG 2 cut(s) 791, 852
BfoI RGCGCY 1 cut(s) 296
BfuAI ACCTGC 2 cut(s) 48, 430
BglI GCCNNNNNGGC 7 cut(s) 1004, 1066, 1097, 1189, 1252, 1284, 1414
BlpI GCTNAGC 1 cut(s) 384
Bme18I GGWCC 3 cut(s) 119, 173, 778
BmgBI CACGTC 3 cut(s) 1028, 1136, 1310
BmgT120I GGNCC 4 cut(s) 119, 129, 173, 778
BmiI GGNNCC 5 cut(s) 435, 552, 1039, 1193, 1451
BmsI GCATC 1 cut(s) 326
BpiI GAAGAC 3 cut(s) 167, 1130, 1304
BplI GAGNNNNNCTC 2 cut(s) 1320, 1352
BpmI CTGGAG 1 cut(s) 1126
Bpu10I CCTNAGC 2 cut(s) 570, 1055
Bpu1102I GCTNAGC 1 cut(s) 384
BpuEI CTTGAG 3 cut(s) 17, 1103, 1444
BpuMI CCSGG 5 cut(s) 132, 1252, 1358, 1414, 1447
BsaAI YACGTR 1 cut(s) 189
BsaJI CCNNGG 5 cut(s) 72, 105, 693, 1215, 1370
BsaWI WCCGGW 2 cut(s) 116, 759
Bsc4I CCNNNNNNNGG 2 cut(s) 855, 873
Bse1I ACTGG 1 cut(s) 755
Bse3DI GCAATG 2 cut(s) 279, 395
BseBI CCWGG 7 cut(s) 194, 215, 253, 1105, 1284, 1317, 1372
BseDI CCNNGG 5 cut(s) 72, 105, 693, 1215, 1370
BseGI GGATG 2 cut(s) 263, 1502
BseLI CCNNNNNNNGG 2 cut(s) 855, 873
BseMI GCAATG 2 cut(s) 279, 395
BseMII CTCAG 6 cut(s) 116, 299, 375, 438, 1069, 1287
BseNI ACTGG 1 cut(s) 755
BseRI GAGGAG 2 cut(s) 23, 682
BseYI CCCAGC 2 cut(s) 1455, 1487
BsgI GTGCAG 1 cut(s) 45
Bsh1236I CGCG 4 cut(s) 74, 1004, 1066, 1097
BshFI GGCC 6 cut(s) 130, 533, 836, 1060, 1278, 1375
BshNI GGYRCC 4 cut(s) 433, 1037, 1191, 1449
BsiHKAI GWGCWC 4 cut(s) 341, 692, 986, 1268
BslI CCNNNNNNNGG 2 cut(s) 855, 873
BsmAI GTCTC 2 cut(s) 774, 789
BsmI GAATGC 1 cut(s) 1481
BsnI GGCC 6 cut(s) 130, 533, 836, 1060, 1278, 1375
Bsp1286I GDGCHC 4 cut(s) 341, 692, 986, 1268
Bsp143I GATC 1 cut(s) 724
Bsp1720I GCTNAGC 1 cut(s) 384
BspANI GGCC 6 cut(s) 130, 533, 836, 1060, 1278, 1375
BspCNI CTCAG 6 cut(s) 117, 298, 376, 439, 1068, 1286
BspFNI CGCG 4 cut(s) 74, 1004, 1066, 1097
BspLI GGNNCC 5 cut(s) 435, 552, 1039, 1193, 1451
BspMI ACCTGC 2 cut(s) 48, 430
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 4 cut(s) 433, 1037, 1191, 1449
BsrBI CCGCTC 6 cut(s) 1085, 1117, 1177, 1208, 1402, 1435
BsrDI GCAATG 2 cut(s) 279, 395
BsrI ACTGG 1 cut(s) 755
BssECI CCNNGG 5 cut(s) 72, 105, 693, 1215, 1370
BssMI GATC 1 cut(s) 724
BssNAI GTATAC 1 cut(s) 361
BssSI CACGAG 2 cut(s) 979, 1166
BssT1I CCWWGG 2 cut(s) 105, 1215
Bst1107I GTATAC 1 cut(s) 361
Bst2BI CACGAG 2 cut(s) 979, 1166
Bst2UI CCWGG 7 cut(s) 194, 215, 253, 1105, 1284, 1317, 1372
Bst4CI ACNGT 4 cut(s) 166, 497, 782, 802
Bst6I CTCTTC 1 cut(s) 12
BstBAI YACGTR 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 258, 820
BstDEI CTNAG 9 cut(s) 125, 285, 384, 447, 570, 765, 918, 1055, 1273
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 263, 1502
BstFNI CGCG 4 cut(s) 74, 1004, 1066, 1097
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 1 cut(s) 727
BstMAI GTCTC 2 cut(s) 774, 789
BstMBI GATC 1 cut(s) 724
BstNI CCWGG 7 cut(s) 194, 215, 253, 1105, 1284, 1317, 1372
BstUI CGCG 4 cut(s) 74, 1004, 1066, 1097
BstV2I GAAGAC 3 cut(s) 167, 1130, 1304
BstXI CCANNNNNNTGG 1 cut(s) 214
BstZ17I GTATAC 1 cut(s) 361
BsuRI GGCC 6 cut(s) 130, 533, 836, 1060, 1278, 1375
BtgI CCRYGG 1 cut(s) 72
BtrI CACGTC 3 cut(s) 1028, 1136, 1310
BtsCI GGATG 2 cut(s) 263, 1502
BtsI GCAGTG 2 cut(s) 507, 867
BtsIMutI CAGTG 4 cut(s) 122, 502, 507, 867
BveI ACCTGC 2 cut(s) 48, 430
Cac8I GCNNGC 2 cut(s) 258, 820
CfoI GCGC 1 cut(s) 295
Cfr13I GGNCC 4 cut(s) 119, 129, 173, 778
Cfr42I CCGCGG 1 cut(s) 75
CpoI CGGWCCG 1 cut(s) 778
Csp6I GTAC 8 cut(s) 37, 190, 370, 434, 458, 487, 523, 783
CspI CGGWCCG 1 cut(s) 778
CviAII CATG 3 cut(s) 346, 405, 995
CviQI GTAC 8 cut(s) 37, 190, 370, 434, 458, 487, 523, 783
DdeI CTNAG 9 cut(s) 125, 285, 384, 447, 570, 765, 918, 1055, 1273
DpnI GATC 1 cut(s) 726
DpnII GATC 1 cut(s) 724
EaeI YGGCCR 1 cut(s) 834
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
EciI GGCGGA 4 cut(s) 20, 762, 805, 1356
Ecl136II GAGCTC 3 cut(s) 690, 984, 1266
Eco130I CCWWGG 2 cut(s) 105, 1215
Eco147I AGGCCT 3 cut(s) 1060, 1278, 1375
Eco24I GRGCYC 3 cut(s) 692, 986, 1268
Eco47I GGWCC 3 cut(s) 119, 173, 778
Eco53kI GAGCTC 3 cut(s) 690, 984, 1266
EcoICRI GAGCTC 3 cut(s) 690, 984, 1266
EcoRII CCWGG 7 cut(s) 192, 213, 251, 1103, 1282, 1315, 1370
EcoT14I CCWWGG 2 cut(s) 105, 1215
EcoT38I GRGCYC 3 cut(s) 692, 986, 1268
ErhI CCWWGG 2 cut(s) 105, 1215
FaeI CATG 3 cut(s) 349, 408, 998
FatI CATG 3 cut(s) 345, 404, 994
FauI CCCGC 3 cut(s) 211, 1122, 1440
FblI GTMKAC 2 cut(s) 237, 360
FokI GGATG 2 cut(s) 250, 1489
FriOI GRGCYC 3 cut(s) 692, 986, 1268
FspBI CTAG 2 cut(s) 791, 852
GlaI GCGC 1 cut(s) 294
GsaI CCCAGC 2 cut(s) 1459, 1491
GsuI CTGGAG 1 cut(s) 1126
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 6 cut(s) 130, 533, 836, 1060, 1278, 1375
HhaI GCGC 1 cut(s) 295
Hin1II CATG 3 cut(s) 349, 408, 998
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 3 cut(s) 140, 242, 1017
HphI GGTGA 1 cut(s) 709
Hpy166II GTNNAC 5 cut(s) 122, 238, 361, 785, 936
Hpy188I TCNGA 3 cut(s) 288, 322, 481
Hpy188III TCNNGA 4 cut(s) 1120, 1166, 1349, 1380
Hpy8I GTNNAC 5 cut(s) 122, 238, 361, 785, 936
Hpy99I CGWCG 1 cut(s) 242
HpyCH4III ACNGT 4 cut(s) 166, 497, 782, 802
HpyCH4IV ACGT 4 cut(s) 188, 1027, 1135, 1309
HpyCH4V TGCA 7 cut(s) 43, 62, 317, 404, 470, 507, 974
HpyF3I CTNAG 9 cut(s) 125, 285, 384, 447, 570, 765, 918, 1055, 1273
HpySE526I ACGT 4 cut(s) 188, 1027, 1135, 1309
Hsp92II CATG 3 cut(s) 349, 408, 998
HspAI GCGC 1 cut(s) 293
KpnI GGTACC 1 cut(s) 437
KspI CCGCGG 1 cut(s) 75
Kzo9I GATC 1 cut(s) 724
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 7 cut(s) 152, 336, 647, 695, 1107, 1271, 1360
LweI GCATC 1 cut(s) 326
MaeI CTAG 2 cut(s) 791, 852
MaeII ACGT 4 cut(s) 188, 1027, 1135, 1309
MaeIII GTNAC 1 cut(s) 247
MalI GATC 1 cut(s) 726
MbiI CCGCTC 6 cut(s) 1085, 1117, 1177, 1208, 1402, 1435
MboI GATC 1 cut(s) 724
MboII GAAGA 5 cut(s) 29, 172, 1012, 1130, 1304
MhlI GDGCHC 4 cut(s) 341, 692, 986, 1268
MlsI TGGCCA 1 cut(s) 836
MluCI AATT 6 cut(s) 44, 564, 640, 668, 750, 827
MluNI TGGCCA 1 cut(s) 836
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 2 cut(s) 151, 578
Mox20I TGGCCA 1 cut(s) 836
MroXI GAANNNNTTC 1 cut(s) 600
MscI TGGCCA 1 cut(s) 836
MseI TTAA 1 cut(s) 1519
MslI CAYNNNNRTG 1 cut(s) 344
Msp20I TGGCCA 1 cut(s) 836
MspA1I CMGCKG 3 cut(s) 28, 74, 146
Mva1269I GAATGC 1 cut(s) 1481
MvaI CCWGG 7 cut(s) 194, 215, 253, 1105, 1284, 1317, 1372
MvnI CGCG 4 cut(s) 74, 1004, 1066, 1097
NciI CCSGG 5 cut(s) 132, 1252, 1358, 1414, 1447
NdeII GATC 1 cut(s) 724
NlaIII CATG 3 cut(s) 349, 408, 998
NlaIV GGNNCC 5 cut(s) 435, 552, 1039, 1193, 1451
NmuCI GTSAC 1 cut(s) 247
PaeR7I CTCGAG 4 cut(s) 306, 1151, 1326, 1390
PaqCI CACCTGC 1 cut(s) 430
PceI AGGCCT 3 cut(s) 1060, 1278, 1375
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 1481
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 2 cut(s) 242, 1017
PflMI CCANNNNNTGG 1 cut(s) 873
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
Ppu21I YACGTR 1 cut(s) 189
Psp124BI GAGCTC 3 cut(s) 692, 986, 1268
Psp6I CCWGG 7 cut(s) 192, 213, 251, 1103, 1282, 1315, 1370
PspFI CCCAGC 2 cut(s) 1455, 1487
PspGI CCWGG 7 cut(s) 192, 213, 251, 1103, 1282, 1315, 1370
PspN4I GGNNCC 5 cut(s) 435, 552, 1039, 1193, 1451
PspPI GGNCC 4 cut(s) 119, 129, 173, 778
PspXI VCTCGAGB 4 cut(s) 306, 1151, 1326, 1390
RsaI GTAC 8 cut(s) 38, 191, 371, 435, 459, 488, 524, 784
RsaNI GTAC 8 cut(s) 37, 190, 370, 434, 458, 487, 523, 783
RseI CAYNNNNRTG 1 cut(s) 344
Rsr2I CGGWCCG 1 cut(s) 778
RsrII CGGWCCG 1 cut(s) 778
SacI GAGCTC 3 cut(s) 692, 986, 1268
SacII CCGCGG 1 cut(s) 75
SalI GTCGAC 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 1519
Sau3AI GATC 1 cut(s) 724
Sau96I GGNCC 4 cut(s) 119, 129, 173, 778
SchI GAGTC 1 cut(s) 149
SduI GDGCHC 4 cut(s) 341, 692, 986, 1268
SfaNI GCATC 1 cut(s) 326
Sfr274I CTCGAG 4 cut(s) 306, 1151, 1326, 1390
Sfr303I CCGCGG 1 cut(s) 75
SgrBI CCGCGG 1 cut(s) 75
SinI GGWCC 3 cut(s) 119, 173, 778
SlaI CTCGAG 4 cut(s) 306, 1151, 1326, 1390
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 7 cut(s) 32, 306, 1118, 1151, 1326, 1390, 1423
SmoI CTYRAG 7 cut(s) 32, 306, 1118, 1151, 1326, 1390, 1423
Sse9I AATT 6 cut(s) 44, 564, 640, 668, 750, 827
SseBI AGGCCT 3 cut(s) 1060, 1278, 1375
SspMI CTAG 2 cut(s) 791, 852
SstI GAGCTC 3 cut(s) 692, 986, 1268
StuI AGGCCT 3 cut(s) 1060, 1278, 1375
StyI CCWWGG 2 cut(s) 105, 1215
TaaI ACNGT 4 cut(s) 166, 497, 782, 802
TaiI ACGT 4 cut(s) 191, 1030, 1138, 1312
TaqI TCGA 7 cut(s) 237, 307, 744, 1152, 1327, 1391, 1510
TasI AATT 6 cut(s) 44, 564, 640, 668, 750, 827
TatI WGTACW 1 cut(s) 486
TauI GCSGC 6 cut(s) 31, 74, 305, 1007, 1069, 1100
TfiI GAWTC 2 cut(s) 242, 1017
Tru1I TTAA 1 cut(s) 1519
Tru9I TTAA 1 cut(s) 1519
TscAI CASTG 4 cut(s) 129, 502, 514, 874
TseFI GTSAC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 2 cut(s) 252, 690
TspGWI ACGGA 1 cut(s) 491
TspRI CASTG 4 cut(s) 129, 502, 514, 874
Van91I CCANNNNNTGG 1 cut(s) 873
VpaK11BI GGWCC 3 cut(s) 119, 173, 778
XapI RAATTY 2 cut(s) 750, 827
XhoI CTCGAG 4 cut(s) 306, 1151, 1326, 1390
XmiI GTMKAC 2 cut(s) 237, 360
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 2 cut(s) 791, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.