Rmu_sc0001952.1_g000001

Glutamyl endopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001952.1
OG0002809
Physical Location & Seq
Forward (+)
1 .. 2982
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1498 bp
tccccgcatcctgtttgataggtcatcggaagatgtgtactccgatcctggctcccctatgctgcggagaactcctgctggcacatatgtgcttgcaaaagtgaagaaagaaaatgatgaaggcacttatttactactgaatggaaatggtgctacaccagaggggaatatcccattcctcgatttgtttgacataaatacaggaagtaaagaacgaatatggaagagtgacaaagaaaagtattatgagggtgttgttgccttaatgtctgatgagaaagaaggagatcttcccatcaataccttgaaaatattgacttccaaagagtcaaaaactgaaaacacccaatattatatcctaagctggccggaaaagaaagcatgtcaaattacaaatttccctcatccatatccacagctggcatcattgcagaaagagatggtcaggtaccagagaaaggatggggttcaactaactgcaacactatatcttccaccaggctacgatccatccagagatggccctcttccatgtttggtttggtcttatcctggaaaattcaaaagcaaagatgctgccggacaagttcgtggttctcctaatgaatttgctgggataggtccaacatcagccctgctctggatggctcgaaggtttgccattttatctggaccaactattcctattattggtgagggtgatgatgaagcaaatgataggtatgtggagcaattggtagcaagtgcagaggctgctgtggaggaagtcattcgacgtggagtggctcatccaaagaaaattgctattgggggacattcatatggggcattcatgactgcaaacctactggcacatgctcctcatctttttgcttgtggaattgctcgttcgggtgcttacaacagaacacttactcctttcggttttcagaatgaggagagaactctttgggaggctactagtacttatgtggagatgagtcctttcatgtcagctaataaaattaagaagccagtcttgcttatccatggagaggaagacaataatccagggactttaaccatgcagtccgatcgtttctttaatgctctgaaaggtcatggtgctctttgccgccttgtgattcttccctttgagagccatggctatgcggcaagagagagtatcattcatgtgctatgggaaactggcaggtggttgcaaaaatattgtgtgtcagacacttctgatgtaaatgttaatcaagatgcatgtaaagacaatataggcacaggatctacaaactctgaaagcaaaccagtggctactggcggaggcagtggctcagaattgtcaaattctgagcatgaaggaaatacattgctgatcttggctgctgaggagtataagtttgcacatcattgtcgtgtacaaactatgtcagaacagcatgatgctcgcccctgtgaaagcgcaatagcaatatgcttcctcaggtgcaatttgcatttgtattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

498

Amino Acids

55.09

Weight (kDa)

6.01

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 249 - 403 7.4e-23 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1183
Acc36I ACCTGC 1 cut(s) 1183
Acc65I GGTACC 1 cut(s) 448
AccB1I GGYRCC 1 cut(s) 448
AciI CCGC 5 cut(s) 5, 65, 1115, 1152, 1312
AclWI GGATC 3 cut(s) 39, 501, 1283
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 4 cut(s) 395, 558, 606, 1337
AfaI GTAC 4 cut(s) 39, 450, 965, 1411
AfiI CCNNNNNNNGG 4 cut(s) 458, 640, 690, 1034
AgsI TTSAA 3 cut(s) 308, 471, 563
AhlI ACTAGT 1 cut(s) 960
AjiI CACGTC 1 cut(s) 777
AjnI CCWGG 4 cut(s) 47, 497, 551, 1049
AleI CACNNNNGTG 1 cut(s) 87
AloI GAACNNNNNNTCC 2 cut(s) 899, 931
AluBI AGCT 3 cut(s) 364, 419, 996
AluI AGCT 3 cut(s) 364, 419, 996
Alw21I GWGCWC 1 cut(s) 1109
AlwI GGATC 3 cut(s) 39, 501, 1283
AlwNI CAGNNNCTG 1 cut(s) 753
AoxI GGCC 2 cut(s) 366, 521
ApeKI GCWGC 4 cut(s) 62, 576, 753, 1374
ApoI RAATTY 4 cut(s) 395, 558, 606, 1337
Asp700I GAANNNNTTC 1 cut(s) 769
Asp718I GGTACC 1 cut(s) 448
AspLEI GCGC 1 cut(s) 1455
AspS9I GGNCC 3 cut(s) 522, 621, 672
AsuHPI GGTGA 2 cut(s) 705, 711
AvaII GGWCC 2 cut(s) 621, 672
AxyI CCTNAGG 1 cut(s) 1473
BanI GGYRCC 1 cut(s) 448
BbsI GAAGAC 1 cut(s) 1045
Bbv12I GWGCWC 1 cut(s) 1109
BbvCI CCTCAGC 1 cut(s) 1378
BbvI GCAGC 4 cut(s) 49, 563, 740, 1361
BccI CCATC 6 cut(s) 303, 434, 456, 513, 518, 638
BcgI CGANNNNNNTGC 4 cut(s) 1056, 1090, 1419, 1453
BciT130I CCWGG 4 cut(s) 49, 499, 553, 1051
BcuI ACTAGT 1 cut(s) 960
BfaI CTAG 1 cut(s) 961
BfuAI ACCTGC 1 cut(s) 1183
BglII AGATCT 1 cut(s) 287
BisI GCNGC 6 cut(s) 63, 577, 754, 1115, 1153, 1375
BlsI GCNGC 6 cut(s) 64, 578, 755, 1116, 1154, 1376
BmcAI AGTACT 1 cut(s) 965
Bme1390I CCNGG 4 cut(s) 49, 499, 553, 1051
Bme18I GGWCC 2 cut(s) 621, 672
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 3 cut(s) 522, 621, 672
BmiI GGNNCC 2 cut(s) 53, 450
BmrFI CCNGG 4 cut(s) 49, 499, 553, 1051
BmsI GCATC 5 cut(s) 16, 432, 563, 1238, 1424
BpiI GAAGAC 1 cut(s) 1045
Bpu10I CCTNAGC 2 cut(s) 360, 1378
BsaJI CCNNGG 3 cut(s) 1028, 1050, 1142
BsaXI ACNNNNNCTCC 4 cut(s) 899, 929, 1374, 1404
Bsc4I CCNNNNNNNGG 4 cut(s) 458, 640, 690, 1034
Bse1I ACTGG 5 cut(s) 853, 1014, 1193, 1299, 1313
Bse21I CCTNAGG 1 cut(s) 1473
Bse3DI GCAATG 2 cut(s) 426, 1359
BseBI CCWGG 4 cut(s) 49, 499, 553, 1051
BseDI CCNNGG 3 cut(s) 1028, 1050, 1142
BseGI GGATG 6 cut(s) 7, 404, 467, 510, 649, 788
BseLI CCNNNNNNNGG 4 cut(s) 458, 640, 690, 1034
BseMI GCAATG 2 cut(s) 426, 1359
BseMII CTCAG 4 cut(s) 1333, 1339, 1369, 1487
BseNI ACTGG 5 cut(s) 853, 1014, 1193, 1299, 1313
BseRI GAGGAG 3 cut(s) 850, 951, 1395
BseXI GCAGC 4 cut(s) 49, 563, 740, 1361
BseYI CCCAGC 1 cut(s) 612
BsgI GTGCAG 1 cut(s) 766
Bsh1285I CGRYCG 1 cut(s) 1076
BshFI GGCC 2 cut(s) 368, 523
BshNI GGYRCC 1 cut(s) 448
BsiEI CGRYCG 1 cut(s) 1076
BsiHKAI GWGCWC 1 cut(s) 1109
BsiSI CCGG 2 cut(s) 369, 580
BslFI GGGAC 2 cut(s) 826, 1067
BslI CCNNNNNNNGG 4 cut(s) 458, 640, 690, 1034
BsmFI GGGAC 2 cut(s) 826, 1067
BsmI GAATGC 1 cut(s) 828
BsnI GGCC 2 cut(s) 368, 523
Bsp1286I GDGCHC 1 cut(s) 1109
Bsp1407I TGTACA 1 cut(s) 1409
Bsp143I GATC 6 cut(s) 44, 287, 506, 1073, 1275, 1366
Bsp19I CCATGG 2 cut(s) 1028, 1142
BspACI CCGC 5 cut(s) 5, 65, 1115, 1152, 1312
BspANI GGCC 2 cut(s) 368, 523
BspCNI CTCAG 4 cut(s) 1334, 1338, 1370, 1486
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 2 cut(s) 53, 450
BspMI ACCTGC 1 cut(s) 1183
BspPI GGATC 3 cut(s) 39, 501, 1283
BspT107I GGYRCC 1 cut(s) 448
BsrDI GCAATG 2 cut(s) 426, 1359
BsrGI TGTACA 1 cut(s) 1409
BsrI ACTGG 5 cut(s) 853, 1014, 1193, 1299, 1313
BssECI CCNNGG 3 cut(s) 1028, 1050, 1142
BssMI GATC 6 cut(s) 44, 287, 506, 1073, 1275, 1366
BssT1I CCWWGG 2 cut(s) 1028, 1142
Bst2UI CCWGG 4 cut(s) 49, 499, 553, 1051
Bst6I CTCTTC 2 cut(s) 219, 532
BstAPI GCANNNNNTGC 1 cut(s) 753
BstAUI TGTACA 1 cut(s) 1409
BstC8I GCNNGC 5 cut(s) 80, 94, 366, 421, 1439
BstDEI CTNAG 5 cut(s) 360, 1325, 1342, 1378, 1473
BstDSI CCRYGG 2 cut(s) 1028, 1142
BstF5I GGATG 6 cut(s) 7, 404, 467, 510, 649, 788
BstHHI GCGC 1 cut(s) 1455
BstKTI GATC 6 cut(s) 47, 290, 509, 1076, 1278, 1369
BstMBI GATC 6 cut(s) 44, 287, 506, 1073, 1275, 1366
BstMCI CGRYCG 1 cut(s) 1076
BstMWI GCNNNNNNNGC 2 cut(s) 753, 1019
BstNI CCWGG 4 cut(s) 49, 499, 553, 1051
BstNSI RCATGY 3 cut(s) 385, 858, 1255
BstSCI CCNGG 4 cut(s) 47, 497, 551, 1049
BstV1I GCAGC 4 cut(s) 49, 563, 740, 1361
BstV2I GAAGAC 1 cut(s) 1045
BstX2I RGATCY 2 cut(s) 287, 1275
BstYI RGATCY 2 cut(s) 287, 1275
Bsu36I CCTNAGG 1 cut(s) 1473
BsuRI GGCC 2 cut(s) 368, 523
BtgI CCRYGG 2 cut(s) 1028, 1142
BtrI CACGTC 1 cut(s) 777
BtsCI GGATG 6 cut(s) 7, 404, 467, 510, 649, 788
BtsI GCAGTG 1 cut(s) 1325
BtsIMutI CAGTG 2 cut(s) 1306, 1325
BveI ACCTGC 1 cut(s) 1183
Cac8I GCNNGC 5 cut(s) 80, 94, 366, 421, 1439
CaiI CAGNNNCTG 1 cut(s) 753
CciI TCATGA 1 cut(s) 832
CfoI GCGC 1 cut(s) 1455
Cfr13I GGNCC 3 cut(s) 522, 621, 672
Csp6I GTAC 4 cut(s) 38, 449, 964, 1410
CviQI GTAC 4 cut(s) 38, 449, 964, 1410
DdeI CTNAG 5 cut(s) 360, 1325, 1342, 1378, 1473
DpnI GATC 6 cut(s) 46, 289, 508, 1075, 1277, 1368
DpnII GATC 6 cut(s) 44, 287, 506, 1073, 1275, 1366
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 219, 532
EarI CTCTTC 2 cut(s) 219, 532
EciI GGCGGA 1 cut(s) 1327
Eco130I CCWWGG 2 cut(s) 1028, 1142
Eco47I GGWCC 2 cut(s) 621, 672
Eco81I CCTNAGG 1 cut(s) 1473
EcoRII CCWGG 4 cut(s) 47, 497, 551, 1049
EcoT14I CCWWGG 2 cut(s) 1028, 1142
EcoT22I ATGCAT 1 cut(s) 1253
ErhI CCWWGG 2 cut(s) 1028, 1142
FalI AAGNNNNNCTT 4 cut(s) 274, 306, 1002, 1034
FaqI GGGAC 2 cut(s) 826, 1067
FauI CCCGC 1 cut(s) 12
FauNDI CATATG 2 cut(s) 86, 821
Fnu4HI GCNGC 6 cut(s) 63, 577, 754, 1115, 1153, 1375
FokI GGATG 5 cut(s) 391, 474, 497, 656, 775
Fsp4HI GCNGC 6 cut(s) 63, 577, 754, 1115, 1153, 1375
FspBI CTAG 1 cut(s) 961
GlaI GCGC 1 cut(s) 1454
GluI GCNGC 6 cut(s) 63, 577, 754, 1115, 1153, 1375
GsaI CCCAGC 1 cut(s) 616
HaeIII GGCC 2 cut(s) 368, 523
HapII CCGG 2 cut(s) 369, 580
HhaI GCGC 1 cut(s) 1455
Hin6I GCGC 1 cut(s) 1453
HinP1I GCGC 1 cut(s) 1453
HinfI GANTC 3 cut(s) 327, 980, 1124
HpaII CCGG 2 cut(s) 369, 580
HphI GGTGA 2 cut(s) 705, 711
Hpy166II GTNNAC 2 cut(s) 38, 1410
Hpy188III TCNNGA 5 cut(s) 514, 641, 670, 833, 1245
Hpy8I GTNNAC 2 cut(s) 38, 1410
Hpy99I CGWCG 1 cut(s) 778
HpyAV CCTTC 4 cut(s) 114, 276, 646, 1344
HpyCH4IV ACGT 1 cut(s) 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 753, 1019
HpyF3I CTNAG 5 cut(s) 360, 1325, 1342, 1378, 1473
HpySE526I ACGT 1 cut(s) 776
HspAI GCGC 1 cut(s) 1453
KpnI GGTACC 1 cut(s) 452
Kzo9I GATC 6 cut(s) 44, 287, 506, 1073, 1275, 1366
LmnI GCTCC 3 cut(s) 57, 728, 863
Lsp1109I GCAGC 4 cut(s) 49, 563, 740, 1361
LweI GCATC 5 cut(s) 16, 432, 563, 1238, 1424
MaeI CTAG 1 cut(s) 961
MaeII ACGT 1 cut(s) 776
MaeIII GTNAC 1 cut(s) 228
MalI GATC 6 cut(s) 46, 289, 508, 1075, 1277, 1368
MboI GATC 6 cut(s) 44, 287, 506, 1073, 1275, 1366
MboII GAAGA 8 cut(s) 42, 116, 236, 282, 483, 519, 1050, 1119
MfeI CAATTG 1 cut(s) 732
MflI RGATCY 2 cut(s) 287, 1275
MhlI GDGCHC 1 cut(s) 1109
MlyI GAGTC 2 cut(s) 336, 989
MmeI TCCRAC 1 cut(s) 648
Mph1103I ATGCAT 1 cut(s) 1253
MroXI GAANNNNTTC 1 cut(s) 769
MseI TTAA 5 cut(s) 264, 1006, 1059, 1084, 1240
MslI CAYNNNNRTG 5 cut(s) 87, 820, 1147, 1173, 1405
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 2 cut(s) 369, 580
MspR9I CCNGG 4 cut(s) 49, 499, 553, 1051
MunI CAATTG 1 cut(s) 732
Mva1269I GAATGC 1 cut(s) 828
MvaI CCWGG 4 cut(s) 49, 499, 553, 1051
MwoI GCNNNNNNNGC 2 cut(s) 753, 1019
NcoI CCATGG 2 cut(s) 1028, 1142
NdeI CATATG 2 cut(s) 86, 821
NdeII GATC 6 cut(s) 44, 287, 506, 1073, 1275, 1366
NlaIV GGNNCC 2 cut(s) 53, 450
NmuCI GTSAC 1 cut(s) 228
NsiI ATGCAT 1 cut(s) 1253
NspI RCATGY 3 cut(s) 385, 858, 1255
OliI CACNNNNGTG 1 cut(s) 87
PagI TCATGA 1 cut(s) 832
PaqCI CACCTGC 1 cut(s) 1183
PctI GAATGC 1 cut(s) 828
PdmI GAANNNNTTC 1 cut(s) 769
PfeI GAWTC 1 cut(s) 1124
PfoI TCCNGGA 1 cut(s) 551
PkrI GCNGC 6 cut(s) 64, 578, 755, 1116, 1154, 1376
Ple19I CGATCG 1 cut(s) 1076
PleI GAGTC 2 cut(s) 335, 988
PpsI GAGTC 2 cut(s) 335, 988
Psp6I CCWGG 4 cut(s) 47, 497, 551, 1049
PspFI CCCAGC 1 cut(s) 612
PspGI CCWGG 4 cut(s) 47, 497, 551, 1049
PspN4I GGNNCC 2 cut(s) 53, 450
PspPI GGNCC 3 cut(s) 522, 621, 672
PstNI CAGNNNCTG 1 cut(s) 753
PsuI RGATCY 2 cut(s) 287, 1275
PvuI CGATCG 1 cut(s) 1076
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 4 cut(s) 39, 450, 965, 1411
RsaNI GTAC 4 cut(s) 38, 449, 964, 1410
RseI CAYNNNNRTG 5 cut(s) 87, 820, 1147, 1173, 1405
SaqAI TTAA 5 cut(s) 264, 1006, 1059, 1084, 1240
SatI GCNGC 6 cut(s) 63, 577, 754, 1115, 1153, 1375
Sau3AI GATC 6 cut(s) 44, 287, 506, 1073, 1275, 1366
Sau96I GGNCC 3 cut(s) 522, 621, 672
ScaI AGTACT 1 cut(s) 965
SchI GAGTC 2 cut(s) 336, 989
ScrFI CCNGG 4 cut(s) 49, 499, 553, 1051
SduI GDGCHC 1 cut(s) 1109
SfaNI GCATC 5 cut(s) 16, 432, 563, 1238, 1424
SinI GGWCC 2 cut(s) 621, 672
SmiMI CAYNNNNRTG 5 cut(s) 87, 820, 1147, 1173, 1405
SpeI ACTAGT 1 cut(s) 960
SsiI CCGC 5 cut(s) 5, 65, 1115, 1152, 1312
SspI AATATT 3 cut(s) 313, 351, 1209
SspMI CTAG 1 cut(s) 961
StyD4I CCNGG 4 cut(s) 47, 497, 551, 1049
StyI CCWWGG 2 cut(s) 1028, 1142
TaiI ACGT 1 cut(s) 779
TaqI TCGA 3 cut(s) 181, 650, 773
TatI WGTACW 3 cut(s) 37, 963, 1409
TauI GCSGC 2 cut(s) 1117, 1155
TfiI GAWTC 1 cut(s) 1124
Tru1I TTAA 5 cut(s) 264, 1006, 1059, 1084, 1240
Tru9I TTAA 5 cut(s) 264, 1006, 1059, 1084, 1240
TscAI CASTG 2 cut(s) 1306, 1325
TseFI GTSAC 1 cut(s) 228
TseI GCWGC 4 cut(s) 62, 576, 753, 1374
Tsp45I GTSAC 1 cut(s) 228
TspDTI ATGAA 8 cut(s) 133, 619, 721, 808, 821, 977, 1161, 1363
TspRI CASTG 2 cut(s) 1306, 1325
VpaK11BI GGWCC 2 cut(s) 621, 672
XapI RAATTY 4 cut(s) 395, 558, 606, 1337
XceI RCATGY 3 cut(s) 385, 858, 1255
XcmI CCANNNNNNNNNTGG 2 cut(s) 459, 538
XmnI GAANNNNTTC 1 cut(s) 769
XspI CTAG 1 cut(s) 961
ZrmI AGTACT 1 cut(s) 965
Zsp2I ATGCAT 1 cut(s) 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.