Rmu_sc0002070.1_g000018

Small subunit processome component 20 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002070.1
OG0014821
Physical Location & Seq
Reverse (-)
96336 .. 103369
7034 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1935 bp
atgggtaactgggatttgaattttgtggtttcagtattggattcgaacattggagatgtggatttgggattgaaggaaaaaatgtcaaaattggggctaggaagtgaaggggagggtcttgcaagttccaccagtgttcaggcatcgtttgtaagaggtgttcttgagaaaactggagttgagccacaagtggagaggattagtaaatacgaaggtgctgctttgcggcaaaaattagacactttgaggctgtggttatatctaagtggggattttgagttcaggcctcatccaaatgaatgttctggaagtgatcttgatggagatatttactttgtttgctgggaccccgagttgatttctcgcaatcaaatccaacctatggattataccccagcaccaactataaagatggatcatgatgtcacaattgagtgcccagtttacttctttattaatcggatgcaattgcatgctctgttttcatactccaaagttgcatcaaagtacccttcaaggctgctggcatgctgtcgacatgctgccgccatgctgccgagcccttcatacgacgaagaagatgttatatgtctgttactcatcttctgtgaaagagtgcaaatgggagtgcaaagctttaagttactagatggatcacctgaactgtcaaggatccaatgttttctgagtggcacggtcagtaattgggttgggcttttaaagggtgtcgagaatggggatttatcatctacttcaattcatgaagctgacttggccctgctgtggggaagcattaactgctttctgcaaatagtagattcccaagaaggtccatccttgttattcgatttgacagatgcaatcgatcaacccctgatgattgaagatgatatggctatggattctttaaagatgtttgctgacaaaatgtgccactcagacggagggattcgtgcttcaactattagaatattatgccactttggaactttaagttgcgatgagccagttctgaaaaagatgaaaactgaactttctcctagttctcatgtcgataacaaagactttaatgttattcagcttcttctgtcaatcgagtcaactcctctttcaatttctaccagcagaaaagttacactattgatttctagaatacagatgggcctctctgctggaaaaatatctgaagcttatcttccccttgttgtaaatgggatgattggtatcttccataaccggtttagtcatcttcgaaatcctgtatcagagtgccttgcagttctgatcagccaaagtaatggacttgtatgggaaaattttcacaactattttgagcaatgccagtctgtatttcaagcatctattgatcaagttggccaagttgatactgtgctgtccaacaagtcaagtgttatccatttgagagagactgagagagagagagagagaggggtaggcggcggtgcagttcaacccgatctcaagcccatcctcctcctcctccgatctcaagacccggcggtgcagttcaacccgatctcaagcccatcctcctcctcctccgatctcaagacccgtcctccttctccgcctacagttcaacccccatcctcctccgatcgctgcagttcaacaacccatcctccttctccggtaaagccctcctcctccgatctggccacccatcctcctcctcctccgatctcaagacccatcctccttctccgcctacagttcaacccgatctcaagcccatcctcctcctcctccgatctcaagacccgtcctccttctcctccgatctcaagacccgtcctccttctccgcctacagttcaacccccatcctcctccgatcgctgcagttcaacccgtctgcagttcacacatcgagacccatcctccttcgccgttttgcttctgggtagcatcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DRIM

Protein Analysis

644

Amino Acids

70.83

Weight (kDa)

5.54

Isoelectric Point (pI)

59.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP PF05183 95 - 160 1.9e-16 RNA dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 382
AccI GTMKAC 1 cut(s) 535
AciI CCGC 8 cut(s) 226, 546, 1458, 1461, 1520, 1589, 1726, 1825
AclWI GGATC 4 cut(s) 423, 661, 667, 680
AcoI YGGCCR 2 cut(s) 1375, 1677
AcsI RAATTY 2 cut(s) 19, 1315
AcuI CTGAAG 1 cut(s) 1206
AfaI GTAC 1 cut(s) 509
AfiI CCNNNNNNNGG 3 cut(s) 382, 783, 784
AgeI ACCGGT 1 cut(s) 1236
AjuI GAANNNNNNNTTGG 2 cut(s) 286, 318
AloI GAACNNNNNNTCC 2 cut(s) 263, 295
AluBI AGCT 4 cut(s) 636, 767, 1081, 1190
AluI AGCT 4 cut(s) 636, 767, 1081, 1190
Alw26I GTCTC 2 cut(s) 1421, 1886
AlwI GGATC 4 cut(s) 423, 661, 667, 680
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 5 cut(s) 284, 774, 1162, 1375, 1677
ApeKI GCWGC 6 cut(s) 218, 520, 542, 553, 1623, 1859
ApoI RAATTY 2 cut(s) 19, 1315
AseI ATTAAT 1 cut(s) 456
AsiGI ACCGGT 1 cut(s) 1236
Asp700I GAANNNNTTC 1 cut(s) 1317
AspS9I GGNCC 4 cut(s) 346, 775, 830, 1162
AsuC2I CCSGG 1 cut(s) 1517
AsuHPI GGTGA 1 cut(s) 648
AsuII TTCGAA 2 cut(s) 44, 1252
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 2 cut(s) 346, 830
BaeGI GKGCMC 1 cut(s) 440
BalI TGGCCA 2 cut(s) 1377, 1679
BamHI GGATCC 1 cut(s) 672
BanII GRGCYC 1 cut(s) 563
BbvI GCAGC 6 cut(s) 205, 507, 529, 540, 1610, 1846
BceAI ACGGC 1 cut(s) 1894
BcgI CGANNNNNNTGC 2 cut(s) 200, 234
BclI TGATCA 2 cut(s) 1284, 1366
BcnI CCSGG 1 cut(s) 1517
BcoDI GTCTC 2 cut(s) 1421, 1886
BfaI CTAG 4 cut(s) 98, 647, 1041, 1149
BfmI CTRYAG 6 cut(s) 1593, 1624, 1730, 1829, 1860, 1876
BisI GCNGC 9 cut(s) 219, 227, 521, 543, 546, 554, 1459, 1624, 1860
BlsI GCNGC 9 cut(s) 220, 228, 522, 544, 547, 555, 1460, 1625, 1861
Bme1390I CCNGG 1 cut(s) 1517
Bme18I GGWCC 2 cut(s) 346, 830
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 4 cut(s) 346, 775, 830, 1162
BmiI GGNNCC 3 cut(s) 347, 348, 674
BmrFI CCNGG 1 cut(s) 1517
BmrI ACTGGG 2 cut(s) 19, 434
BmsI GCATC 5 cut(s) 152, 453, 509, 847, 1367
BmuI ACTGGG 2 cut(s) 19, 434
BpmI CTGGAG 1 cut(s) 195
Bpu14I TTCGAA 2 cut(s) 44, 1252
BpuEI CTTGAG 9 cut(s) 185, 1466, 1494, 1525, 1553, 1690, 1732, 1760, 1789
BpuMI CCSGG 1 cut(s) 1517
Bsa29I ATCGAT 1 cut(s) 864
BsaBI GATNNNNATC 1 cut(s) 1223
BsaI GGTCTC 1 cut(s) 1886
BsaWI WCCGGW 2 cut(s) 1236, 1651
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 783, 784
Bse118I RCCGGY 1 cut(s) 1236
Bse1I ACTGG 6 cut(s) 14, 132, 178, 440, 1007, 1342
Bse3DI GCAATG 1 cut(s) 1343
Bse8I GATNNNNATC 1 cut(s) 1223
BseCI ATCGAT 1 cut(s) 864
BseJI GATNNNNATC 1 cut(s) 1223
BseLI CCNNNNNNNGG 3 cut(s) 382, 783, 784
BseMI GCAATG 1 cut(s) 1343
BseMII CTCAG 3 cut(s) 677, 951, 1422
BseNI ACTGG 6 cut(s) 14, 132, 178, 440, 1007, 1342
BseSI GKGCMC 1 cut(s) 440
BseXI GCAGC 6 cut(s) 205, 507, 529, 540, 1610, 1846
BseYI CCCAGC 2 cut(s) 342, 394
BsgI GTGCAG 2 cut(s) 1485, 1544
Bsh1285I CGRYCG 2 cut(s) 1621, 1857
BshFI GGCC 5 cut(s) 286, 776, 1164, 1377, 1679
BshTI ACCGGT 1 cut(s) 1236
BshVI ATCGAT 1 cut(s) 864
BsiEI CGRYCG 2 cut(s) 1621, 1857
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 3 cut(s) 1237, 1517, 1652
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 3 cut(s) 382, 783, 784
BsmAI GTCTC 2 cut(s) 1421, 1886
BsmFI GGGAC 1 cut(s) 359
BsnI GGCC 5 cut(s) 286, 776, 1164, 1377, 1679
Bso31I GGTCTC 1 cut(s) 1886
BsoBI CYCGRG 1 cut(s) 350
Bsp119I TTCGAA 2 cut(s) 44, 1252
Bsp1286I GDGCHC 2 cut(s) 440, 563
BspACI CCGC 8 cut(s) 226, 546, 1458, 1461, 1520, 1589, 1726, 1825
BspANI GGCC 5 cut(s) 286, 776, 1164, 1377, 1679
BspCNI CTCAG 3 cut(s) 678, 950, 1423
BspDI ATCGAT 1 cut(s) 864
BspHI TCATGA 3 cut(s) 418, 760, 1931
BspLI GGNNCC 3 cut(s) 347, 348, 674
BspMAI CTGCAG 3 cut(s) 1628, 1864, 1880
BspPI GGATC 4 cut(s) 423, 661, 667, 680
BspT104I TTCGAA 2 cut(s) 44, 1252
BspTNI GGTCTC 1 cut(s) 1886
BsrDI GCAATG 1 cut(s) 1343
BsrFI RCCGGY 1 cut(s) 1236
BsrI ACTGG 6 cut(s) 14, 132, 178, 440, 1007, 1342
BssAI RCCGGY 1 cut(s) 1236
Bst4CI ACNGT 6 cut(s) 666, 697, 1390, 1597, 1734, 1833
BstAPI GCANNNNNTGC 1 cut(s) 798
BstBI TTCGAA 2 cut(s) 44, 1252
BstC8I GCNNGC 3 cut(s) 474, 525, 529
BstDEI CTNAG 4 cut(s) 263, 686, 937, 1431
BstMAI GTCTC 2 cut(s) 1421, 1886
BstMCI CGRYCG 2 cut(s) 1621, 1857
BstMWI GCNNNNNNNGC 2 cut(s) 773, 798
BstNSI RCATGY 3 cut(s) 476, 531, 542
BstSCI CCNGG 1 cut(s) 1515
BstSFI CTRYAG 6 cut(s) 1593, 1624, 1730, 1829, 1860, 1876
BstSLI GKGCMC 1 cut(s) 440
BstV1I GCAGC 6 cut(s) 205, 507, 529, 540, 1610, 1846
BstX2I RGATCY 1 cut(s) 672
BstXI CCANNNNNNTGG 1 cut(s) 1298
BstYI RGATCY 1 cut(s) 672
Bsu15I ATCGAT 1 cut(s) 864
BsuRI GGCC 5 cut(s) 286, 776, 1164, 1377, 1679
BsuTUI ATCGAT 1 cut(s) 864
BtgZI GCGATG 1 cut(s) 1014
BtsIMutI CAGTG 1 cut(s) 139
Cac8I GCNNGC 3 cut(s) 474, 525, 529
CciI TCATGA 3 cut(s) 418, 760, 1931
Cfr10I RCCGGY 1 cut(s) 1236
Cfr13I GGNCC 4 cut(s) 346, 775, 830, 1162
ClaI ATCGAT 1 cut(s) 864
Csp6I GTAC 1 cut(s) 508
CspAI ACCGGT 1 cut(s) 1236
CviAII CATG 8 cut(s) 419, 473, 528, 539, 550, 761, 1049, 1932
CviQI GTAC 1 cut(s) 508
DdeI CTNAG 4 cut(s) 263, 686, 937, 1431
DraI TTTAAA 2 cut(s) 720, 909
EaeI YGGCCR 2 cut(s) 1375, 1677
EciI GGCGGA 3 cut(s) 1578, 1715, 1814
Eco147I AGGCCT 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 563
Eco31I GGTCTC 1 cut(s) 1886
Eco47I GGWCC 2 cut(s) 346, 830
Eco57I CTGAAG 1 cut(s) 1206
Eco88I CYCGRG 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 563
FaeI CATG 8 cut(s) 422, 476, 531, 542, 553, 764, 1052, 1935
FalI AAGNNNNNCTT 2 cut(s) 1179, 1211
FaqI GGGAC 1 cut(s) 359
FatI CATG 8 cut(s) 418, 472, 527, 538, 549, 760, 1048, 1931
FbaI TGATCA 2 cut(s) 1284, 1366
FblI GTMKAC 1 cut(s) 535
Fnu4HI GCNGC 9 cut(s) 219, 227, 521, 543, 546, 554, 1459, 1624, 1860
FriOI GRGCYC 1 cut(s) 563
Fsp4HI GCNGC 9 cut(s) 219, 227, 521, 543, 546, 554, 1459, 1624, 1860
FspBI CTAG 4 cut(s) 98, 647, 1041, 1149
GluI GCNGC 9 cut(s) 219, 227, 521, 543, 546, 554, 1459, 1624, 1860
GsaI CCCAGC 2 cut(s) 346, 398
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 5 cut(s) 286, 776, 1164, 1377, 1679
HapII CCGG 3 cut(s) 1237, 1517, 1652
Hin1II CATG 8 cut(s) 422, 476, 531, 542, 553, 764, 1052, 1935
HincII GTYRAC 2 cut(s) 536, 1101
HindII GTYRAC 2 cut(s) 536, 1101
HindIII AAGCTT 2 cut(s) 634, 1188
HinfI GANTC 5 cut(s) 41, 818, 902, 949, 1097
HpaII CCGG 3 cut(s) 1237, 1517, 1652
HphI GGTGA 1 cut(s) 648
Hpy166II GTNNAC 4 cut(s) 445, 536, 1101, 1883
Hpy8I GTNNAC 4 cut(s) 445, 536, 1101, 1883
Hpy99I CGWCG 1 cut(s) 575
HpyCH4III ACNGT 6 cut(s) 666, 697, 1390, 1597, 1734, 1833
HpyF10VI GCNNNNNNNGC 2 cut(s) 773, 798
HpyF3I CTNAG 4 cut(s) 263, 686, 937, 1431
Hsp92II CATG 8 cut(s) 422, 476, 531, 542, 553, 764, 1052, 1935
KflI GGGWCCC 1 cut(s) 346
Ksp22I TGATCA 2 cut(s) 1284, 1366
Lsp1109I GCAGC 6 cut(s) 205, 507, 529, 540, 1610, 1846
LweI GCATC 5 cut(s) 152, 453, 509, 847, 1367
MaeI CTAG 4 cut(s) 98, 647, 1041, 1149
MaeIII GTNAC 5 cut(s) 5, 424, 594, 642, 1132
MboII GAAGA 8 cut(s) 587, 590, 595, 896, 1076, 1187, 1219, 1241
MfeI CAATTG 2 cut(s) 429, 467
MflI RGATCY 1 cut(s) 672
MhlI GDGCHC 2 cut(s) 440, 563
MlsI TGGCCA 2 cut(s) 1377, 1679
MluCI AATT 9 cut(s) 19, 89, 233, 429, 467, 703, 756, 1113, 1315
MluNI TGGCCA 2 cut(s) 1377, 1679
MlyI GAGTC 1 cut(s) 1106
MmeI TCCRAC 2 cut(s) 400, 1422
Mox20I TGGCCA 2 cut(s) 1377, 1679
MroXI GAANNNNTTC 1 cut(s) 1317
MscI TGGCCA 2 cut(s) 1377, 1679
MseI TTAA 7 cut(s) 456, 639, 719, 795, 908, 992, 1068
MslI CAYNNNNRTG 1 cut(s) 294
Msp20I TGGCCA 2 cut(s) 1377, 1679
MspI CCGG 3 cut(s) 1237, 1517, 1652
MspR9I CCNGG 1 cut(s) 1517
MunI CAATTG 2 cut(s) 429, 467
MwoI GCNNNNNNNGC 2 cut(s) 773, 798
NciI CCSGG 1 cut(s) 1517
NlaIII CATG 8 cut(s) 422, 476, 531, 542, 553, 764, 1052, 1935
NlaIV GGNNCC 3 cut(s) 347, 348, 674
NmeAIII GCCGAG 1 cut(s) 582
NmuCI GTSAC 1 cut(s) 424
NspI RCATGY 3 cut(s) 476, 531, 542
NspV TTCGAA 2 cut(s) 44, 1252
PaeI GCATGC 2 cut(s) 476, 531
PagI TCATGA 3 cut(s) 418, 760, 1931
PceI AGGCCT 1 cut(s) 286
PdmI GAANNNNTTC 1 cut(s) 1317
PfeI GAWTC 4 cut(s) 41, 818, 902, 949
PflMI CCANNNNNTGG 1 cut(s) 382
PinAI ACCGGT 1 cut(s) 1236
PkrI GCNGC 9 cut(s) 220, 228, 522, 544, 547, 555, 1460, 1625, 1861
Ple19I CGATCG 2 cut(s) 1621, 1857
PleI GAGTC 1 cut(s) 1105
PpsI GAGTC 1 cut(s) 1105
PpuMI RGGWCCY 1 cut(s) 346
PshBI ATTAAT 1 cut(s) 456
Psp5II RGGWCCY 1 cut(s) 346
PspFI CCCAGC 2 cut(s) 342, 394
PspN4I GGNNCC 3 cut(s) 347, 348, 674
PspPI GGNCC 4 cut(s) 346, 775, 830, 1162
PspPPI RGGWCCY 1 cut(s) 346
PsrI GAACNNNNNNTAC 2 cut(s) 144, 176
PstI CTGCAG 3 cut(s) 1628, 1864, 1880
PsuI RGATCY 1 cut(s) 672
PvuI CGATCG 2 cut(s) 1621, 1857
RsaI GTAC 1 cut(s) 509
RsaNI GTAC 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 294
SalI GTCGAC 1 cut(s) 534
SaqAI TTAA 7 cut(s) 456, 639, 719, 795, 908, 992, 1068
SatI GCNGC 9 cut(s) 219, 227, 521, 543, 546, 554, 1459, 1624, 1860
Sau96I GGNCC 4 cut(s) 346, 775, 830, 1162
SchI GAGTC 1 cut(s) 1106
ScrFI CCNGG 1 cut(s) 1517
SduI GDGCHC 2 cut(s) 440, 563
SetI ASST 9 cut(s) 160, 217, 382, 638, 661, 769, 832, 1083, 1192
SfaNI GCATC 5 cut(s) 152, 453, 509, 847, 1367
SfcI CTRYAG 6 cut(s) 1593, 1624, 1730, 1829, 1860, 1876
SfuI TTCGAA 2 cut(s) 44, 1252
SinI GGWCC 2 cut(s) 346, 830
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 9 cut(s) 164, 1481, 1509, 1540, 1568, 1705, 1747, 1775, 1804
SmoI CTYRAG 9 cut(s) 164, 1481, 1509, 1540, 1568, 1705, 1747, 1775, 1804
SphI GCATGC 2 cut(s) 476, 531
Sse9I AATT 9 cut(s) 19, 89, 233, 429, 467, 703, 756, 1113, 1315
SseBI AGGCCT 1 cut(s) 286
SsiI CCGC 8 cut(s) 226, 546, 1458, 1461, 1520, 1589, 1726, 1825
SspI AATATT 1 cut(s) 972
SspMI CTAG 4 cut(s) 98, 647, 1041, 1149
StuI AGGCCT 1 cut(s) 286
StyD4I CCNGG 1 cut(s) 1515
TaaI ACNGT 6 cut(s) 666, 697, 1390, 1597, 1734, 1833
TaqI TCGA 9 cut(s) 44, 535, 729, 846, 864, 1053, 1095, 1252, 1890
TasI AATT 9 cut(s) 19, 89, 233, 429, 467, 703, 756, 1113, 1315
TauI GCSGC 3 cut(s) 229, 548, 1461
TfiI GAWTC 4 cut(s) 41, 818, 902, 949
Tru1I TTAA 7 cut(s) 456, 639, 719, 795, 908, 992, 1068
Tru9I TTAA 7 cut(s) 456, 639, 719, 795, 908, 992, 1068
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 6 cut(s) 218, 520, 542, 553, 1623, 1859
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 6 cut(s) 312, 474, 555, 749, 777, 1037
TspGWI ACGGA 1 cut(s) 957
TspRI CASTG 1 cut(s) 139
Van91I CCANNNNNTGG 1 cut(s) 382
VpaK11BI GGWCC 2 cut(s) 346, 830
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 2 cut(s) 19, 1315
XbaI TCTAGA 1 cut(s) 1148
XceI RCATGY 3 cut(s) 476, 531, 542
XmiI GTMKAC 1 cut(s) 535
XmnI GAANNNNTTC 1 cut(s) 1317
XspI CTAG 4 cut(s) 98, 647, 1041, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.