Rroxscaffold_5G00350550

K homology RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014821
Physical Location & Seq
Forward (+)
24684436 .. 24696265
11830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00350550.1

Sequence Viewer

Length: 708 bp
ATGTTGATATCGGAAACAATGGTTGGTGGGTTGATTTGTAGATGCGGCTCCAACATTTCAAGGATTAGAAATGAGTCCGGCGCAATGATCAAGGTTTATGGGGGGAAAGGTGAACAAAAGCATAGGCAAATTCAGTTTTGTGGTAATGCTCGGCGGGAAAGGAAAGTTACGAGCAATGGCAGAGAATGTGACTGTGTGGATCACCGAGCTCGGTCACCACTATTTTTCAGGCCACTCATTTTGGCTCAAGGTGCCCAAGAAAATATCAACTCCATAGAGAAATATATAGTGTCCAAAGAGCAATGCGTCACAAATAGGCCACCAAAGAATAAGGACAAGTCCTCCATTAAGATTATAGCCCTTGGCACCTCCAACACAACCAAAACTGCCCATAGATTATCCTCCAAATATGAAATGAAGTGCAAAGAAGTCCAACCAACCGGAGTGAACAAAACTACCTCCGGCGACCTCTTCGGTGAAACCAACCCGAACGATTCGCCTCCTTGCTCCGGTCGTCTCGTGGTGGTCTCCATCGACGATTCTCATGCACGGCGGCGCCGCAAGTCGGCCCGGTTGAGGAAGCGGACCAGGCCGGAAAACACGGCTCCGACGTCGGCCGGGCTTCGATTTTTCTTGGGTGTGACAGGTGGATACTTCTCAAGTGCGACCACCCTATTGTTAGGGGCTAAGGAACTCCTTGACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

235

Amino Acids

25.98

Weight (kDa)

10.03

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 1 - 50 1.9e-06 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 614
AccB1I GGYRCC 3 cut(s) 251, 365, 555
AciI CCGC 5 cut(s) 45, 154, 553, 559, 583
AclWI GGATC 1 cut(s) 207
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 1 cut(s) 129
AcyI GRCGYC 2 cut(s) 556, 611
AfiI CCNNNNNNNGG 3 cut(s) 509, 565, 576
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 1 cut(s) 60
AjnI CCWGG 1 cut(s) 587
AjuI GAANNNNNNNTTGG 1 cut(s) 38
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 2 cut(s) 521, 532
AlwI GGATC 1 cut(s) 207
AoxI GGCC 5 cut(s) 230, 317, 567, 590, 615
ApoI RAATTY 1 cut(s) 129
AspLEI GCGC 2 cut(s) 83, 558
AspS9I GGNCC 2 cut(s) 568, 585
AsuC2I CCSGG 2 cut(s) 571, 619
AsuHPI GGTGA 4 cut(s) 122, 194, 207, 488
AvaII GGWCC 1 cut(s) 585
BaeGI GKGCMC 1 cut(s) 256
BanI GGYRCC 3 cut(s) 251, 365, 555
BanII GRGCYC 1 cut(s) 211
BauI CACGAG 1 cut(s) 518
Bbv12I GWGCWC 1 cut(s) 211
BccI CCATC 1 cut(s) 539
BceAI ACGGC 2 cut(s) 566, 618
BcgI CGANNNNNNTGC 2 cut(s) 527, 561
BciT130I CCWGG 1 cut(s) 589
BciVI GTATCC 1 cut(s) 644
BclI TGATCA 1 cut(s) 87
BcnI CCSGG 2 cut(s) 571, 619
BcoDI GTCTC 2 cut(s) 521, 532
BfoI RGCGCY 1 cut(s) 559
BfuI GTATCC 1 cut(s) 644
BisI GCNGC 3 cut(s) 46, 554, 559
BlsI GCNGC 3 cut(s) 47, 555, 560
Bme1390I CCNGG 3 cut(s) 571, 589, 619
Bme18I GGWCC 1 cut(s) 585
BmgT120I GGNCC 2 cut(s) 568, 585
BmiI GGNNCC 5 cut(s) 49, 253, 367, 557, 606
BmrFI CCNGG 3 cut(s) 571, 589, 619
BmsI GCATC 1 cut(s) 32
Bpu10I CCTNAGC 1 cut(s) 687
BpuEI CTTGAG 2 cut(s) 231, 643
BpuMI CCSGG 2 cut(s) 571, 619
BsaHI GRCGYC 2 cut(s) 556, 611
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 1 cut(s) 361
BsaWI WCCGGW 2 cut(s) 440, 509
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 509, 565, 576
Bse3DI GCAATG 3 cut(s) 90, 181, 308
BseBI CCWGG 1 cut(s) 589
BseDI CCNNGG 1 cut(s) 361
BseLI CCNNNNNNNGG 3 cut(s) 509, 565, 576
BseMI GCAATG 3 cut(s) 90, 181, 308
BseSI GKGCMC 1 cut(s) 256
BseX3I CGGCCG 1 cut(s) 615
Bsh1285I CGRYCG 2 cut(s) 514, 618
BshFI GGCC 5 cut(s) 232, 319, 569, 592, 617
BshNI GGYRCC 3 cut(s) 251, 365, 555
BsiEI CGRYCG 2 cut(s) 514, 618
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 7 cut(s) 78, 441, 462, 510, 571, 593, 618
BslI CCNNNNNNNGG 3 cut(s) 509, 565, 576
BsmAI GTCTC 2 cut(s) 521, 532
BsmBI CGTCTC 1 cut(s) 521
BsnI GGCC 5 cut(s) 232, 319, 569, 592, 617
Bso31I GGTCTC 1 cut(s) 532
Bsp1286I GDGCHC 2 cut(s) 211, 256
Bsp143I GATC 2 cut(s) 87, 199
BspACI CCGC 5 cut(s) 45, 154, 553, 559, 583
BspANI GGCC 5 cut(s) 232, 319, 569, 592, 617
BspLI GGNNCC 5 cut(s) 49, 253, 367, 557, 606
BspPI GGATC 1 cut(s) 207
BspT107I GGYRCC 3 cut(s) 251, 365, 555
BspTNI GGTCTC 1 cut(s) 532
BsrDI GCAATG 3 cut(s) 90, 181, 308
BssECI CCNNGG 1 cut(s) 361
BssMI GATC 2 cut(s) 87, 199
BssNI GRCGYC 2 cut(s) 556, 611
BssSI CACGAG 1 cut(s) 518
BssT1I CCWWGG 1 cut(s) 361
Bst2BI CACGAG 1 cut(s) 518
Bst2UI CCWGG 1 cut(s) 589
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 476
BstACI GRCGYC 2 cut(s) 556, 611
BstDEI CTNAG 1 cut(s) 687
BstEII GGTNACC 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 559
BstHHI GCGC 2 cut(s) 83, 558
BstKTI GATC 2 cut(s) 90, 202
BstMAI GTCTC 2 cut(s) 521, 532
BstMBI GATC 2 cut(s) 87, 199
BstMCI CGRYCG 2 cut(s) 514, 618
BstMWI GCNNNNNNNGC 2 cut(s) 251, 589
BstNI CCWGG 1 cut(s) 589
BstNSI RCATGY 1 cut(s) 706
BstPI GGTNACC 1 cut(s) 213
BstSCI CCNGG 3 cut(s) 569, 587, 617
BstSLI GKGCMC 1 cut(s) 256
BstZI CGGCCG 1 cut(s) 615
BsuI GTATCC 1 cut(s) 644
BsuRI GGCC 5 cut(s) 232, 319, 569, 592, 617
CfoI GCGC 2 cut(s) 83, 558
Cfr13I GGNCC 2 cut(s) 568, 585
CseI GACGC 1 cut(s) 295
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviAII CATG 2 cut(s) 545, 703
DdeI CTNAG 1 cut(s) 687
DinI GGCGCC 1 cut(s) 557
DpnI GATC 2 cut(s) 89, 201
DpnII GATC 2 cut(s) 87, 199
EaeI YGGCCR 1 cut(s) 615
EagI CGGCCG 1 cut(s) 615
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
Ecl136II GAGCTC 1 cut(s) 209
EclXI CGGCCG 1 cut(s) 615
Eco130I CCWWGG 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 211
Eco31I GGTCTC 1 cut(s) 532
Eco32I GATATC 1 cut(s) 9
Eco47I GGWCC 1 cut(s) 585
Eco52I CGGCCG 1 cut(s) 615
Eco53kI GAGCTC 1 cut(s) 209
Eco91I GGTNACC 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 587
EcoRV GATATC 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 211
EgeI GGCGCC 1 cut(s) 557
EheI GGCGCC 1 cut(s) 557
ErhI CCWWGG 1 cut(s) 361
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 2 cut(s) 548, 706
FatI CATG 2 cut(s) 544, 702
FauI CCCGC 1 cut(s) 147
FbaI TGATCA 1 cut(s) 87
Fnu4HI GCNGC 3 cut(s) 46, 554, 559
FriOI GRGCYC 1 cut(s) 211
Fsp4HI GCNGC 3 cut(s) 46, 554, 559
GlaI GCGC 2 cut(s) 82, 557
GluI GCNGC 3 cut(s) 46, 554, 559
HaeII RGCGCY 1 cut(s) 559
HaeIII GGCC 5 cut(s) 232, 319, 569, 592, 617
HapII CCGG 7 cut(s) 78, 441, 462, 510, 571, 593, 618
HgaI GACGC 1 cut(s) 295
HhaI GCGC 2 cut(s) 83, 558
Hin1I GRCGYC 2 cut(s) 556, 611
Hin1II CATG 2 cut(s) 548, 706
Hin6I GCGC 2 cut(s) 81, 556
HinP1I GCGC 2 cut(s) 81, 556
HinfI GANTC 3 cut(s) 74, 494, 539
HpaII CCGG 7 cut(s) 78, 441, 462, 510, 571, 593, 618
HphI GGTGA 4 cut(s) 122, 194, 207, 488
Hpy166II GTNNAC 2 cut(s) 113, 448
Hpy188I TCNGA 2 cut(s) 13, 609
Hpy8I GTNNAC 2 cut(s) 113, 448
Hpy99I CGWCG 3 cut(s) 539, 613, 616
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 611
HpyCH4V TGCA 2 cut(s) 423, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 589
HpyF3I CTNAG 1 cut(s) 687
HpySE526I ACGT 1 cut(s) 611
Hsp92I GRCGYC 2 cut(s) 556, 611
Hsp92II CATG 2 cut(s) 548, 706
HspAI GCGC 2 cut(s) 81, 556
KasI GGCGCC 1 cut(s) 555
Ksp22I TGATCA 1 cut(s) 87
Kzo9I GATC 2 cut(s) 87, 199
LmnI GCTCC 3 cut(s) 53, 512, 610
LweI GCATC 1 cut(s) 32
MaeII ACGT 1 cut(s) 611
MaeIII GTNAC 5 cut(s) 166, 188, 213, 307, 640
MalI GATC 2 cut(s) 89, 201
MboI GATC 2 cut(s) 87, 199
MboII GAAGA 1 cut(s) 463
MhlI GDGCHC 2 cut(s) 211, 256
MluCI AATT 1 cut(s) 129
Mly113I GGCGCC 1 cut(s) 556
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 4 cut(s) 75, 396, 457, 632
MnlI CCTC 7 cut(s) 352, 379, 412, 469, 479, 510, 570
MseI TTAA 1 cut(s) 348
MspI CCGG 7 cut(s) 78, 441, 462, 510, 571, 593, 618
MspR9I CCNGG 3 cut(s) 571, 589, 619
MvaI CCWGG 1 cut(s) 589
MwoI GCNNNNNNNGC 2 cut(s) 251, 589
NarI GGCGCC 1 cut(s) 556
NciI CCSGG 2 cut(s) 571, 619
NdeII GATC 2 cut(s) 87, 199
NlaIII CATG 2 cut(s) 548, 706
NlaIV GGNNCC 5 cut(s) 49, 253, 367, 557, 606
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 4 cut(s) 188, 213, 307, 640
NspI RCATGY 1 cut(s) 706
PciI ACATGT 1 cut(s) 702
PcsI WCGNNNNNNNCGW 1 cut(s) 608
PfeI GAWTC 2 cut(s) 494, 539
PkrI GCNGC 3 cut(s) 47, 555, 560
PleI GAGTC 1 cut(s) 82
PluTI GGCGCC 1 cut(s) 559
PpsI GAGTC 1 cut(s) 82
PscI ACATGT 1 cut(s) 702
Psp124BI GAGCTC 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 587
PspEI GGTNACC 1 cut(s) 213
PspGI CCWGG 1 cut(s) 587
PspN4I GGNNCC 5 cut(s) 49, 253, 367, 557, 606
PspPI GGNCC 2 cut(s) 568, 585
SacI GAGCTC 1 cut(s) 211
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 3 cut(s) 46, 554, 559
Sau3AI GATC 2 cut(s) 87, 199
Sau96I GGNCC 2 cut(s) 568, 585
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 3 cut(s) 571, 589, 619
SduI GDGCHC 2 cut(s) 211, 256
SetI ASST 9 cut(s) 96, 112, 211, 253, 371, 461, 471, 614, 649
SfaNI GCATC 1 cut(s) 32
SfoI GGCGCC 1 cut(s) 557
SinI GGWCC 1 cut(s) 585
SmlI CTYRAG 2 cut(s) 246, 658
SmoI CTYRAG 2 cut(s) 246, 658
Sse9I AATT 1 cut(s) 129
SsiI CCGC 5 cut(s) 45, 154, 553, 559, 583
SspDI GGCGCC 1 cut(s) 555
SstI GAGCTC 1 cut(s) 211
StyD4I CCNGG 3 cut(s) 569, 587, 617
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 1 cut(s) 614
TaqI TCGA 2 cut(s) 534, 625
TaqII GACCGA 1 cut(s) 201
TasI AATT 1 cut(s) 129
TauI GCSGC 3 cut(s) 48, 556, 561
TfiI GAWTC 2 cut(s) 494, 539
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TseFI GTSAC 4 cut(s) 188, 213, 307, 640
Tsp45I GTSAC 4 cut(s) 188, 213, 307, 640
TspDTI ATGAA 2 cut(s) 426, 431
VpaK11BI GGWCC 1 cut(s) 585
XapI RAATTY 1 cut(s) 129
XceI RCATGY 1 cut(s) 706
ZraI GACGTC 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.