Rmu_sc0002235.1_g000065

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002235.1
OG0005127
Physical Location & Seq
Forward (+)
214721 .. 218872
4152 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 699 bp
atgctccaaactcgcctcttcctcttctggctcaacccccgttcagatctaatccccagaaggctacttttcccaaagacaatgattgaaattgatgtaatctttctcgcaaaggcgatgattcgaggcgtggccggaggaggttcgacctggcgtagactcgtggggcgaagggaaggcaaagtgcctcggtgggatacctttggtgagctggttcagccactcgcaagtgcaaggccatggatggtaacccagggaaaccatgagaaggagagcataccattcttgaaggatgggtttgaatcctataattctaggtggaaaatgccgtataaagagagtggatcgagctcgaatctttattactcctttgaagttgcaggtgctcatattatcatgcttggctcgtatactgattatgatgaatattcagatcaatacaagtggttgaaggctgatctatcaaaggtagaccaacaaaagacaccttggttgcttgtgctatttcatgttccctggtataatagtaacaaagctcatcaaggtgaaagggacagcatgatggcagcaatggagccatttctttatgatcatcttcaaatgcgcctatttagaaacccagaggagatcccatggggtcagtttggcgccgatattattgaggagtctactggagtattcactgacaaggacacctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

232

Amino Acids

27.09

Weight (kDa)

6.53

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 60 - 187 3e-10 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 371
Acc36I ACCTGC 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 647
AccI GTMKAC 4 cut(s) 157, 410, 471, 668
AclWI GGATC 2 cut(s) 352, 622
AcoI YGGCCR 1 cut(s) 132
AcyI GRCGYC 1 cut(s) 648
AfiI CCNNNNNNNGG 1 cut(s) 268
AgsI TTSAA 6 cut(s) 89, 289, 302, 374, 451, 599
AjnI CCWGG 3 cut(s) 149, 252, 515
AluBI AGCT 3 cut(s) 211, 351, 536
AluI AGCT 3 cut(s) 211, 351, 536
Alw21I GWGCWC 2 cut(s) 353, 388
AlwI GGATC 2 cut(s) 352, 622
AoxI GGCC 2 cut(s) 132, 236
ApeKI GCWGC 1 cut(s) 566
AspLEI GCGC 2 cut(s) 606, 650
AsuHPI GGTGA 2 cut(s) 218, 557
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 1 cut(s) 353
BauI CACGAG 1 cut(s) 161
Bbv12I GWGCWC 2 cut(s) 353, 388
BbvI GCAGC 1 cut(s) 578
BccI CCATC 3 cut(s) 238, 287, 556
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 3 cut(s) 151, 254, 517
BciVI GTATCC 1 cut(s) 190
BclI TGATCA 1 cut(s) 589
BfaI CTAG 1 cut(s) 315
BfoI RGCGCY 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 371
BfuI GTATCC 1 cut(s) 190
BglII AGATCT 1 cut(s) 46
BisI GCNGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 568
Bme1390I CCNGG 3 cut(s) 151, 254, 517
BmiI GGNNCC 2 cut(s) 576, 649
BmrFI CCNGG 3 cut(s) 151, 254, 517
BpmI CTGGAG 1 cut(s) 693
BsaHI GRCGYC 1 cut(s) 648
BsaJI CCNNGG 7 cut(s) 188, 239, 252, 253, 488, 515, 632
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 676
Bse3DI GCAATG 1 cut(s) 576
BseBI CCWGG 3 cut(s) 151, 254, 517
BseDI CCNNGG 7 cut(s) 188, 239, 252, 253, 488, 515, 632
BseGI GGATG 2 cut(s) 249, 298
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMI GCAATG 1 cut(s) 576
BseNI ACTGG 1 cut(s) 676
BseRI GAGGAG 3 cut(s) 153, 638, 677
BseXI GCAGC 1 cut(s) 578
BshFI GGCC 2 cut(s) 134, 238
BshNI GGYRCC 1 cut(s) 647
BsiHKAI GWGCWC 2 cut(s) 353, 388
BsiSI CCGG 1 cut(s) 135
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 566
BsnI GGCC 2 cut(s) 134, 238
Bsp1286I GDGCHC 2 cut(s) 353, 388
Bsp143I GATC 6 cut(s) 46, 344, 433, 457, 589, 627
Bsp19I CCATGG 2 cut(s) 239, 632
BspANI GGCC 2 cut(s) 134, 238
BspLI GGNNCC 2 cut(s) 576, 649
BspMI ACCTGC 1 cut(s) 371
BspPI GGATC 2 cut(s) 352, 622
BspT107I GGYRCC 1 cut(s) 647
BsrDI GCAATG 1 cut(s) 576
BsrI ACTGG 1 cut(s) 676
BssECI CCNNGG 7 cut(s) 188, 239, 252, 253, 488, 515, 632
BssMI GATC 6 cut(s) 46, 344, 433, 457, 589, 627
BssNAI GTATAC 1 cut(s) 411
BssNI GRCGYC 1 cut(s) 648
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 3 cut(s) 239, 488, 632
Bst1107I GTATAC 1 cut(s) 411
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 3 cut(s) 151, 254, 517
Bst6I CTCTTC 2 cut(s) 23, 29
BstACI GRCGYC 1 cut(s) 648
BstDSI CCRYGG 2 cut(s) 239, 632
BstEII GGTNACC 1 cut(s) 247
BstF5I GGATG 2 cut(s) 249, 298
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 2 cut(s) 606, 650
BstKTI GATC 6 cut(s) 49, 347, 436, 460, 592, 630
BstMBI GATC 6 cut(s) 46, 344, 433, 457, 589, 627
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNI CCWGG 3 cut(s) 151, 254, 517
BstPI GGTNACC 1 cut(s) 247
BstSCI CCNGG 3 cut(s) 149, 252, 515
BstV1I GCAGC 1 cut(s) 578
BstX2I RGATCY 2 cut(s) 46, 627
BstYI RGATCY 2 cut(s) 46, 627
BstZ17I GTATAC 1 cut(s) 411
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 2 cut(s) 134, 238
BtgI CCRYGG 2 cut(s) 239, 632
BtgZI GCGATG 1 cut(s) 131
BtsCI GGATG 2 cut(s) 249, 298
BtsIMutI CAGTG 1 cut(s) 681
BveI ACCTGC 1 cut(s) 371
CfoI GCGC 2 cut(s) 606, 650
CspCI CAANNNNNGTGG 2 cut(s) 425, 460
CviAII CATG 6 cut(s) 240, 263, 397, 509, 559, 633
DinI GGCGCC 1 cut(s) 649
DpnI GATC 6 cut(s) 48, 346, 435, 459, 591, 629
DpnII GATC 6 cut(s) 46, 344, 433, 457, 589, 627
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 2 cut(s) 23, 29
EarI CTCTTC 2 cut(s) 23, 29
Ecl136II GAGCTC 1 cut(s) 351
Eco130I CCWWGG 3 cut(s) 239, 488, 632
Eco24I GRGCYC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 351
Eco91I GGTNACC 1 cut(s) 247
EcoICRI GAGCTC 1 cut(s) 351
EcoO65I GGTNACC 1 cut(s) 247
EcoRII CCWGG 3 cut(s) 149, 252, 515
EcoT14I CCWWGG 3 cut(s) 239, 488, 632
EcoT38I GRGCYC 1 cut(s) 353
EgeI GGCGCC 1 cut(s) 649
EheI GGCGCC 1 cut(s) 649
ErhI CCWWGG 3 cut(s) 239, 488, 632
FaeI CATG 6 cut(s) 243, 266, 400, 512, 562, 636
FaqI GGGAC 1 cut(s) 566
FatI CATG 6 cut(s) 239, 262, 396, 508, 558, 632
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 4 cut(s) 157, 410, 471, 668
Fnu4HI GCNGC 1 cut(s) 567
FokI GGATG 2 cut(s) 256, 305
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 1 cut(s) 567
FspBI CTAG 1 cut(s) 315
GlaI GCGC 2 cut(s) 605, 649
GluI GCNGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 693
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 2 cut(s) 134, 238
HapII CCGG 1 cut(s) 135
HhaI GCGC 2 cut(s) 606, 650
Hin1I GRCGYC 1 cut(s) 648
Hin1II CATG 6 cut(s) 243, 266, 400, 512, 562, 636
Hin6I GCGC 2 cut(s) 604, 648
HinP1I GCGC 2 cut(s) 604, 648
HinfI GANTC 5 cut(s) 121, 159, 302, 355, 665
HpaII CCGG 1 cut(s) 135
HphI GGTGA 2 cut(s) 218, 557
Hpy166II GTNNAC 4 cut(s) 158, 411, 472, 669
Hpy188I TCNGA 2 cut(s) 46, 433
Hpy188III TCNNGA 1 cut(s) 286
Hpy8I GTNNAC 4 cut(s) 158, 411, 472, 669
HpyAV CCTTC 6 cut(s) 54, 165, 170, 262, 283, 445
HpyCH4V TGCA 2 cut(s) 233, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
Hsp92I GRCGYC 1 cut(s) 648
Hsp92II CATG 6 cut(s) 243, 266, 400, 512, 562, 636
HspAI GCGC 2 cut(s) 604, 648
KasI GGCGCC 1 cut(s) 647
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 6 cut(s) 46, 344, 433, 457, 589, 627
LmnI GCTCC 2 cut(s) 9, 574
Lsp1109I GCAGC 1 cut(s) 578
MaeI CTAG 1 cut(s) 315
MaeIII GTNAC 2 cut(s) 247, 527
MalI GATC 6 cut(s) 48, 346, 435, 459, 591, 629
MboI GATC 6 cut(s) 46, 344, 433, 457, 589, 627
MboII GAAGA 3 cut(s) 10, 16, 587
MflI RGATCY 2 cut(s) 46, 627
MhlI GDGCHC 2 cut(s) 353, 388
MluCI AATT 2 cut(s) 90, 310
Mly113I GGCGCC 1 cut(s) 648
MlyI GAGTC 2 cut(s) 153, 674
MnlI CCTC 8 cut(s) 26, 32, 119, 131, 134, 198, 616, 655
MslI CAYNNNNRTG 1 cut(s) 543
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 3 cut(s) 151, 254, 517
MvaI CCWGG 3 cut(s) 151, 254, 517
MwoI GCNNNNNNNGC 1 cut(s) 217
NarI GGCGCC 1 cut(s) 648
NcoI CCATGG 2 cut(s) 239, 632
NdeII GATC 6 cut(s) 46, 344, 433, 457, 589, 627
NlaIII CATG 6 cut(s) 243, 266, 400, 512, 562, 636
NlaIV GGNNCC 2 cut(s) 576, 649
PaqCI CACCTGC 1 cut(s) 371
PasI CCCWGGG 1 cut(s) 253
PfeI GAWTC 3 cut(s) 121, 302, 355
PkrI GCNGC 1 cut(s) 568
PleI GAGTC 2 cut(s) 153, 673
PluTI GGCGCC 1 cut(s) 651
PpsI GAGTC 2 cut(s) 153, 673
Psp124BI GAGCTC 1 cut(s) 353
Psp6I CCWGG 3 cut(s) 149, 252, 515
PspEI GGTNACC 1 cut(s) 247
PspGI CCWGG 3 cut(s) 149, 252, 515
PspN4I GGNNCC 2 cut(s) 576, 649
PsuI RGATCY 2 cut(s) 46, 627
RseI CAYNNNNRTG 1 cut(s) 543
SacI GAGCTC 1 cut(s) 353
SatI GCNGC 1 cut(s) 567
Sau3AI GATC 6 cut(s) 46, 344, 433, 457, 589, 627
SchI GAGTC 2 cut(s) 153, 674
ScrFI CCNGG 3 cut(s) 151, 254, 517
SduI GDGCHC 2 cut(s) 353, 388
SfoI GGCGCC 1 cut(s) 649
SmiMI CAYNNNNRTG 1 cut(s) 543
Sse9I AATT 2 cut(s) 90, 310
SspDI GGCGCC 1 cut(s) 647
SspI AATATT 1 cut(s) 428
SspMI CTAG 1 cut(s) 315
SstI GAGCTC 1 cut(s) 353
StyD4I CCNGG 3 cut(s) 149, 252, 515
StyI CCWWGG 3 cut(s) 239, 488, 632
TaqI TCGA 4 cut(s) 124, 146, 347, 353
TasI AATT 2 cut(s) 90, 310
TfiI GAWTC 3 cut(s) 121, 302, 355
TscAI CASTG 1 cut(s) 688
TseI GCWGC 1 cut(s) 566
TspDTI ATGAA 2 cut(s) 438, 497
TspRI CASTG 1 cut(s) 688
XmiI GTMKAC 4 cut(s) 157, 410, 471, 668
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.