Rmu_sc0002489.1_g000011

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002489.1
OG0008491
Physical Location & Seq
Forward (+)
34113 .. 35879
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1023 bp
atggaagctgtattcgggatgtctctagccaccatcattttgacaagcatggcagccatggctgcatcagaagcatctccagccacatttgtgttcggtgactcgttaacagaagtgggaaacaacaattatttgcagtattctcttgcaaagtcaaattttccttggtacggggtcgattatgaaggtggtcaggctaccgggagattcacaaatggaagaactattgctgcaaagcttgggattccatcaccgccgccttatctctcattgtcaacgaatgatgatgcactgcttaagggggtcaactatgcatctggtggagcaggaatactaaatgacactggactctattttattgagagactgtcctttgatgatcaagtacaaagtttcaacaagacaagggaagtcatcaaggctaagattgggagtgaagctgcaaccaagcttttaactgaagctctctatttcattggaattggcagcaatgattatgtcaataatttcttacaaccctttttacctgatggtcaagagtacactcatgatgactttgtggaactgttaataggcaccttagagcaacaacttaagactctctaccagttgggtgcacgaaagatgatgtttcatggtcttggtccactcggctgcattccttctcagagggttaaatcaaagaaaggccaatgcttgaagaaagtcaacaagtgggtgctggaattcaactctaaagtagagaagctcattgcctctcttaacagacgcctcccaaaagcaaaattctcatttgctgatacatattcagatgttcttgacttaatcagcaaccctaccacatatgacacgagcattggagggctatgcttaccgaattcaaaaatatgcacgaaccgcaaagattatgtattctgggatgcattccacccctcagatgcagcaaatgcagttcttgctgacaaactcttcttgagactattttctaagcctccagcccctgcaccaacaccttctcactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

340

Amino Acids

37.25

Weight (kDa)

7.61

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 323 7.9e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 575
AciI CCGC 3 cut(s) 254, 257, 898
AcsI RAATTY 4 cut(s) 157, 725, 785, 877
AcuI CTGAAG 1 cut(s) 480
AcyI GRCGYC 1 cut(s) 769
AfaI GTAC 3 cut(s) 170, 387, 542
AfiI CCNNNNNNNGG 1 cut(s) 170
AflII CTTAAG 2 cut(s) 296, 593
AgsI TTSAA 4 cut(s) 397, 700, 730, 882
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 6 cut(s) 8, 238, 440, 451, 464, 748
AluI AGCT 6 cut(s) 8, 238, 440, 451, 464, 748
Alw21I GWGCWC 1 cut(s) 619
Alw26I GTCTC 3 cut(s) 27, 358, 970
Alw44I GTGCAC 1 cut(s) 615
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 1 cut(s) 688
ApaLI GTGCAC 1 cut(s) 615
ApeKI GCWGC 7 cut(s) 53, 62, 230, 440, 486, 654, 941
ApoI RAATTY 4 cut(s) 157, 725, 785, 877
AspS9I GGNCC 1 cut(s) 644
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 110, 243
AvaII GGWCC 1 cut(s) 644
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 575
BauI CACGAG 1 cut(s) 850
Bbv12I GWGCWC 1 cut(s) 619
BbvI GCAGC 7 cut(s) 49, 65, 217, 427, 498, 641, 953
BccI CCATC 3 cut(s) 41, 256, 524
BclI TGATCA 1 cut(s) 379
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 3 cut(s) 27, 358, 970
BfaI CTAG 1 cut(s) 26
BfrI CTTAAG 2 cut(s) 296, 593
BisI GCNGC 8 cut(s) 54, 63, 231, 257, 441, 487, 655, 942
BlsI GCNGC 8 cut(s) 55, 64, 232, 258, 442, 488, 656, 943
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 644
BmgT120I GGNCC 1 cut(s) 644
BmiI GGNNCC 1 cut(s) 577
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 6 cut(s) 74, 83, 277, 323, 910, 928
BpmI CTGGAG 2 cut(s) 63, 978
BpuEI CTTGAG 1 cut(s) 994
BpuMI CCSGG 1 cut(s) 202
BsaHI GRCGYC 1 cut(s) 769
BsaJI CCNNGG 2 cut(s) 57, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 349, 607
Bse3DI GCAATG 2 cut(s) 496, 750
BseDI CCNNGG 2 cut(s) 57, 164
BseGI GGATG 2 cut(s) 24, 925
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 2 cut(s) 496, 750
BseMII CTCAG 2 cut(s) 680, 948
BseNI ACTGG 2 cut(s) 349, 607
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 7 cut(s) 49, 65, 217, 427, 498, 641, 953
BsgI GTGCAG 1 cut(s) 987
BshFI GGCC 1 cut(s) 690
BshNI GGYRCC 1 cut(s) 575
BsiHKAI GWGCWC 1 cut(s) 619
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 3 cut(s) 27, 358, 970
BsmI GAATGC 2 cut(s) 657, 923
BsnI GGCC 1 cut(s) 690
Bsp1286I GDGCHC 1 cut(s) 619
Bsp143I GATC 1 cut(s) 379
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 3 cut(s) 254, 257, 898
BspANI GGCC 1 cut(s) 690
BspCNI CTCAG 2 cut(s) 679, 947
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 1 cut(s) 577
BspT107I GGYRCC 1 cut(s) 575
BspTI CTTAAG 2 cut(s) 296, 593
BsrDI GCAATG 2 cut(s) 496, 750
BsrI ACTGG 2 cut(s) 349, 607
BssECI CCNNGG 2 cut(s) 57, 164
BssMI GATC 1 cut(s) 379
BssNI GRCGYC 1 cut(s) 769
BssSI CACGAG 1 cut(s) 850
BssT1I CCWWGG 2 cut(s) 57, 164
Bst2BI CACGAG 1 cut(s) 850
Bst4CI ACNGT 2 cut(s) 369, 567
Bst6I CTCTTC 1 cut(s) 974
BstACI GRCGYC 1 cut(s) 769
BstAFI CTTAAG 2 cut(s) 296, 593
BstAPI GCANNNNNTGC 2 cut(s) 947, 956
BstDEI CTNAG 5 cut(s) 423, 580, 666, 934, 987
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 24, 925
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 3 cut(s) 27, 358, 970
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 7 cut(s) 59, 62, 71, 80, 897, 947, 956
BstSCI CCNGG 1 cut(s) 200
BstSLI GKGCMC 1 cut(s) 619
BstV1I GCAGC 7 cut(s) 49, 65, 217, 427, 498, 641, 953
BsuRI GGCC 1 cut(s) 690
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 24, 925
BtsI GCAGTG 1 cut(s) 290
BtsIMutI CAGTG 2 cut(s) 290, 342
CaiI CAGNNNCTG 1 cut(s) 1001
CciI TCATGA 1 cut(s) 547
Cfr13I GGNCC 1 cut(s) 644
CseI GACGC 1 cut(s) 777
Csp6I GTAC 3 cut(s) 169, 386, 541
CviAII CATG 4 cut(s) 49, 58, 548, 635
CviQI GTAC 3 cut(s) 169, 386, 541
DdeI CTNAG 5 cut(s) 423, 580, 666, 934, 987
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 974
EarI CTCTTC 1 cut(s) 974
Eco130I CCWWGG 2 cut(s) 57, 164
Eco47I GGWCC 1 cut(s) 644
Eco57I CTGAAG 1 cut(s) 480
EcoRI GAATTC 2 cut(s) 725, 877
EcoT14I CCWWGG 2 cut(s) 57, 164
EcoT22I ATGCAT 2 cut(s) 316, 925
ErhI CCWWGG 2 cut(s) 57, 164
FaeI CATG 4 cut(s) 52, 61, 551, 638
FatI CATG 4 cut(s) 48, 57, 547, 634
FauNDI CATATG 1 cut(s) 844
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 8 cut(s) 54, 63, 231, 257, 441, 487, 655, 942
FokI GGATG 2 cut(s) 31, 932
Fsp4HI GCNGC 8 cut(s) 54, 63, 231, 257, 441, 487, 655, 942
FspBI CTAG 1 cut(s) 26
GluI GCNGC 8 cut(s) 54, 63, 231, 257, 441, 487, 655, 942
GsuI CTGGAG 2 cut(s) 63, 978
HaeIII GGCC 1 cut(s) 690
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 777
Hin1I GRCGYC 1 cut(s) 769
Hin1II CATG 4 cut(s) 52, 61, 551, 638
HincII GTYRAC 4 cut(s) 108, 276, 307, 709
HindII GTYRAC 4 cut(s) 108, 276, 307, 709
HindIII AAGCTT 2 cut(s) 236, 449
HinfI GANTC 5 cut(s) 101, 207, 244, 348, 598
HpaI GTTAAC 1 cut(s) 108
HpaII CCGG 1 cut(s) 201
HphI GGTGA 2 cut(s) 110, 243
Hpy166II GTNNAC 7 cut(s) 108, 276, 307, 543, 617, 647, 709
Hpy188I TCNGA 4 cut(s) 70, 669, 811, 937
Hpy188III TCNNGA 5 cut(s) 16, 536, 548, 818, 973
Hpy8I GTNNAC 7 cut(s) 108, 276, 307, 543, 617, 647, 709
HpyAV CCTTC 3 cut(s) 179, 672, 1023
HpyCH4III ACNGT 2 cut(s) 369, 567
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 62, 71, 80, 897, 947, 956
HpyF3I CTNAG 5 cut(s) 423, 580, 666, 934, 987
Hsp92I GRCGYC 1 cut(s) 769
Hsp92II CATG 4 cut(s) 52, 61, 551, 638
Ksp22I TGATCA 1 cut(s) 379
KspAI GTTAAC 1 cut(s) 108
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 1 cut(s) 323
Lsp1109I GCAGC 7 cut(s) 49, 65, 217, 427, 498, 641, 953
LweI GCATC 6 cut(s) 74, 83, 277, 323, 910, 928
MaeI CTAG 1 cut(s) 26
MaeIII GTNAC 1 cut(s) 98
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MboII GAAGA 3 cut(s) 231, 712, 961
MhlI GDGCHC 1 cut(s) 619
MluCI AATT 7 cut(s) 127, 157, 480, 505, 725, 785, 877
MlyI GAGTC 3 cut(s) 95, 342, 592
MnlI CCTC 6 cut(s) 663, 766, 782, 854, 943, 1002
Mph1103I ATGCAT 2 cut(s) 316, 925
MseI TTAA 8 cut(s) 107, 297, 455, 569, 594, 675, 762, 824
MslI CAYNNNNRTG 1 cut(s) 89
MspCI CTTAAG 2 cut(s) 296, 593
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 202
Mva1269I GAATGC 2 cut(s) 657, 923
MwoI GCNNNNNNNGC 7 cut(s) 59, 62, 71, 80, 897, 947, 956
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 844
NdeII GATC 1 cut(s) 379
NlaIII CATG 4 cut(s) 52, 61, 551, 638
NlaIV GGNNCC 1 cut(s) 577
NmeAIII GCCGAG 1 cut(s) 630
NmuCI GTSAC 1 cut(s) 98
NsiI ATGCAT 2 cut(s) 316, 925
OliI CACNNNNGTG 1 cut(s) 89
PagI TCATGA 1 cut(s) 547
PctI GAATGC 2 cut(s) 657, 923
PfeI GAWTC 2 cut(s) 207, 244
PkrI GCNGC 8 cut(s) 55, 64, 232, 258, 442, 488, 656, 943
PleI GAGTC 3 cut(s) 95, 342, 592
PpsI GAGTC 3 cut(s) 95, 342, 592
PspN4I GGNNCC 1 cut(s) 577
PspPI GGNCC 1 cut(s) 644
PstNI CAGNNNCTG 1 cut(s) 1001
RsaI GTAC 3 cut(s) 170, 387, 542
RsaNI GTAC 3 cut(s) 169, 386, 541
RseI CAYNNNNRTG 1 cut(s) 89
SaqAI TTAA 8 cut(s) 107, 297, 455, 569, 594, 675, 762, 824
SatI GCNGC 8 cut(s) 54, 63, 231, 257, 441, 487, 655, 942
Sau3AI GATC 1 cut(s) 379
Sau96I GGNCC 1 cut(s) 644
SchI GAGTC 3 cut(s) 95, 342, 592
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 1 cut(s) 619
SfaNI GCATC 6 cut(s) 74, 83, 277, 323, 910, 928
SinI GGWCC 1 cut(s) 644
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 3 cut(s) 296, 593, 973
SmoI CTYRAG 3 cut(s) 296, 593, 973
Sse9I AATT 7 cut(s) 127, 157, 480, 505, 725, 785, 877
SsiI CCGC 3 cut(s) 254, 257, 898
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 2 cut(s) 57, 164
TaaI ACNGT 2 cut(s) 369, 567
TaqI TCGA 1 cut(s) 177
TasI AATT 7 cut(s) 127, 157, 480, 505, 725, 785, 877
TatI WGTACW 2 cut(s) 385, 540
TauI GCSGC 1 cut(s) 259
TfiI GAWTC 2 cut(s) 207, 244
Tru1I TTAA 8 cut(s) 107, 297, 455, 569, 594, 675, 762, 824
Tru9I TTAA 8 cut(s) 107, 297, 455, 569, 594, 675, 762, 824
TscAI CASTG 2 cut(s) 297, 349
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 7 cut(s) 53, 62, 230, 440, 486, 654, 941
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 3 cut(s) 198, 463, 623
TspRI CASTG 2 cut(s) 297, 349
Vha464I CTTAAG 2 cut(s) 296, 593
VneI GTGCAC 1 cut(s) 615
VpaK11BI GGWCC 1 cut(s) 644
XapI RAATTY 4 cut(s) 157, 725, 785, 877
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 2 cut(s) 316, 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.