Rmu_sc0002741.1_g000021

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002741.1
OG0003099
Physical Location & Seq
Forward (+)
85175 .. 95011
9837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1923 bp
atggcctgcgacaagggattttccagcagttttatgctgctgaagccagaagaagtcggattgtttggtctgtttcacattttgttctccagcaagttagaggaaaagaaatttgtagattgcccggaggggactgtcactgatgagatcagcttcaagcgcagatggctcatatttgtttccatcttggctcaaaagtttctgcaacttgtgtcaaagccactctcagttgtcggatctgggatcgaaatgtgtttgaatcttgtatccgtcaaccgcaatgtttttgtgctcgtgatcaatttcttccgaggggtggtggtaaagccagataagacatcagcaacattcctgtcatttataggaaattttgacaagagggtggagttggatagcaccattaaatatgatgatgccaagtaccatgcagctctctctatgatggcttctaaagcatcctatgagaataatgcctacattgaaactactgttagggatcattggaagatggagttcttaggatctttcgacttctggaatgattatcaacaaaaagccacaacacaagcctttatgcttcgggataagtcttctgaccatgacatcgttgttgtggcctttaggggaaccgaaccgtttgatgcagatgcatggtgctccgactttgacatctcttggtatgagctttcaggggtcggaaaaattcatggcggctttatgaaagctctaggtttacaaaagaatgttggttggcctaaggaaaccaaacaagaagatggtcatccagacttggcttactatgcaattagagacatgctaaacaagcttttgagccaaaatgatcaagctagatacatattgacagggcatagtttgggaggggctttagcaattttgtttccaacagttttggctctccacaatgagacatggttattggagagattgcaaggggtatatacatttgggcaaccaagggtgggagatgaaaagtttggtgagttcatggagaaacagttgcaagagcataactttcagtattttagatttgtttatggtaatgatatggttcctaggttgccttatgatgacaaagccttgatgttcaagcactttgggacatgcctttattataataggcactatgaagtgaaggttgtggaccaggaacctaataagaactacttctccccattgggggcaataccaatgataataaatgcatttttcgagctaattagaagcttcaccgttgcaaaaacaaaggggccagattataaagaagggatttttctcagaattttccggataattggcttggtatttcctgggattccagctcatagcactcaagattatgtgaatgcgaccaggttggggtcctcggacgtcttcctcctaccaaaacacacatcatcccatgaagatgccacgacgtggagcagttcagaagctactggagtcgtcgacgaagggtttaatgttgagctcgatgggcttccatggtttcggacgagcttgccgcacacagctgagtttccgatctccttcaccgcttgctcgaggcagtccgggatgtctagaggggtgagtcaggcacctgcagcgcagcgatcgtctccttccagacgccggaggagcgacgtcagcactttttctggaaaccggcgagagctccgacgaacgagaaaaccttcacttcaggtgctgtacttcttggagcttgatcaggaggttgaggcgagttcaacaagccaagaaactcgagttttggtggccttacaaggagaaatcgacgttgaactcaaaacgggctcaaaccgaaaccgtgcttccgccgccgtaacaggccacgaggtggcgcaaggttgccatcggctgctgcgcaaggtcgaggcgaagttgacggaagaagtttggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

640

Amino Acids

72.64

Weight (kDa)

6.65

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 204 - 363 1.3e-32 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1134, 1278
AarI CACCTGC 1 cut(s) 1609
AatII GACGTC 2 cut(s) 1392, 1647
Acc16I TGCGCA 1 cut(s) 1886
Acc36I ACCTGC 1 cut(s) 1609
AccB1I GGYRCC 1 cut(s) 1597
AccB7I CCANNNNNTGG 2 cut(s) 1437, 1858
AccI GTMKAC 1 cut(s) 1467
AccIII TCCGGA 1 cut(s) 1305
AciI CCGC 6 cut(s) 277, 711, 1523, 1554, 1836, 1839
AclWI GGATC 4 cut(s) 244, 251, 504, 529
AcsI RAATTY 4 cut(s) 110, 367, 702, 1299
AcuI CTGAAG 2 cut(s) 62, 1685
AcyI GRCGYC 3 cut(s) 1389, 1630, 1644
AdeI CACNNNGTG 2 cut(s) 1437, 1858
AfaI GTAC 2 cut(s) 422, 1712
AfiI CCNNNNNNNGG 8 cut(s) 316, 980, 1197, 1198, 1377, 1437, 1632, 1858
AgsI TTSAA 6 cut(s) 157, 259, 482, 1108, 1749, 1802
AjiI CACGTC 1 cut(s) 1437
AjnI CCWGG 3 cut(s) 1164, 1327, 1370
AloI GAACNNNNNNTCC 2 cut(s) 1172, 1204
Alw21I GWGCWC 4 cut(s) 294, 659, 1491, 1677
Alw26I GTCTC 3 cut(s) 804, 920, 1623
AlwI GGATC 4 cut(s) 244, 251, 504, 529
AlwNI CAGNNNCTG 1 cut(s) 1708
Ama87I CYCGRG 2 cut(s) 1561, 1764
Aor13HI TCCGGA 1 cut(s) 1305
AoxI GGCC 6 cut(s) 3, 615, 752, 1268, 1776, 1849
ApeKI GCWGC 6 cut(s) 37, 428, 1604, 1609, 1879, 1882
ApoI RAATTY 4 cut(s) 110, 367, 702, 1299
Asp700I GAANNNNTTC 2 cut(s) 1184, 1693
AspA2I CCTAGG 1 cut(s) 1073
AspLEI GCGC 4 cut(s) 162, 1609, 1864, 1887
AspS9I GGNCC 3 cut(s) 1162, 1268, 1380
AsuC2I CCSGG 2 cut(s) 125, 1573
AsuHPI GGTGA 4 cut(s) 1010, 1240, 1543, 1600
AvaI CYCGRG 2 cut(s) 1561, 1764
AvaII GGWCC 2 cut(s) 1162, 1380
AvrII CCTAGG 1 cut(s) 1073
AxyI CCTNAGG 1 cut(s) 756
BanI GGYRCC 1 cut(s) 1597
BanII GRGCYC 3 cut(s) 1491, 1677, 1817
BauI CACGAG 2 cut(s) 293, 1853
BbsI GAAGAC 2 cut(s) 582, 1384
Bbv12I GWGCWC 4 cut(s) 294, 659, 1491, 1677
BbvI GCAGC 6 cut(s) 24, 440, 1616, 1621, 1866, 1869
BccI CCATC 7 cut(s) 159, 191, 436, 502, 770, 1487, 1881
BceAI ACGGC 1 cut(s) 1826
BciT130I CCWGG 3 cut(s) 1166, 1329, 1372
BciVI GTATCC 1 cut(s) 277
BclI TGATCA 3 cut(s) 297, 841, 1726
BcnI CCSGG 2 cut(s) 125, 1573
BcoDI GTCTC 3 cut(s) 804, 920, 1623
BfaI CTAG 4 cut(s) 728, 849, 1074, 1581
BfmI CTRYAG 1 cut(s) 1602
BfuAI ACCTGC 1 cut(s) 1609
BfuI GTATCC 1 cut(s) 277
BisI GCNGC 9 cut(s) 38, 429, 712, 1523, 1605, 1610, 1839, 1880, 1883
BlnI CCTAGG 1 cut(s) 1073
BlsI GCNGC 9 cut(s) 39, 430, 713, 1524, 1606, 1611, 1840, 1881, 1884
Bme1390I CCNGG 5 cut(s) 125, 1166, 1329, 1372, 1573
Bme18I GGWCC 2 cut(s) 1162, 1380
BmeT110I CYCGRG 2 cut(s) 1561, 1764
BmgBI CACGTC 1 cut(s) 1437
BmgT120I GGNCC 3 cut(s) 1162, 1268, 1380
BmiI GGNNCC 6 cut(s) 628, 1071, 1170, 1269, 1381, 1599
BmrFI CCNGG 5 cut(s) 125, 1166, 1329, 1372, 1573
BmsI GCATC 5 cut(s) 403, 464, 631, 637, 1417
BpiI GAAGAC 2 cut(s) 582, 1384
BpmI CTGGAG 2 cut(s) 73, 1479
BpuEI CTTGAG 1 cut(s) 1335
BpuMI CCSGG 2 cut(s) 125, 1573
BsaBI GATNNNNATC 1 cut(s) 780
BsaHI GRCGYC 3 cut(s) 1389, 1630, 1644
BsaJI CCNNGG 6 cut(s) 310, 974, 1073, 1328, 1383, 1502
BsaWI WCCGGW 1 cut(s) 1305
BsaXI ACNNNNNCTCC 4 cut(s) 1001, 1031, 1172, 1202
Bsc4I CCNNNNNNNGG 8 cut(s) 316, 980, 1197, 1198, 1377, 1437, 1632, 1858
Bse118I RCCGGY 1 cut(s) 1665
Bse1I ACTGG 1 cut(s) 1462
Bse21I CCTNAGG 1 cut(s) 756
Bse3DI GCAATG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 780
BseAI TCCGGA 1 cut(s) 1305
BseBI CCWGG 3 cut(s) 1166, 1329, 1372
BseDI CCNNGG 6 cut(s) 310, 974, 1073, 1328, 1383, 1502
BseGI GGATG 4 cut(s) 455, 781, 1415, 1581
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 8 cut(s) 316, 980, 1197, 1198, 1377, 1437, 1632, 1858
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 3 cut(s) 240, 1309, 1524
BseNI ACTGG 1 cut(s) 1462
BseRI GAGGAG 1 cut(s) 1651
BseXI GCAGC 6 cut(s) 24, 440, 1616, 1621, 1866, 1869
Bsh1285I CGRYCG 1 cut(s) 1616
BshFI GGCC 6 cut(s) 5, 617, 754, 1270, 1778, 1851
BshNI GGYRCC 1 cut(s) 1597
BsiEI CGRYCG 1 cut(s) 1616
BsiHKAI GWGCWC 4 cut(s) 294, 659, 1491, 1677
BsiHKCI CYCGRG 2 cut(s) 1561, 1764
BsiSI CCGG 5 cut(s) 125, 1306, 1572, 1633, 1666
BslFI GGGAC 2 cut(s) 145, 1132
BslI CCNNNNNNNGG 8 cut(s) 316, 980, 1197, 1198, 1377, 1437, 1632, 1858
BsmAI GTCTC 3 cut(s) 804, 920, 1623
BsmBI CGTCTC 1 cut(s) 1623
BsmFI GGGAC 2 cut(s) 145, 1132
BsmI GAATGC 1 cut(s) 1369
BsnI GGCC 6 cut(s) 5, 617, 754, 1270, 1778, 1851
BsoBI CYCGRG 2 cut(s) 1561, 1764
Bsp1286I GDGCHC 5 cut(s) 294, 659, 1491, 1677, 1817
Bsp13I TCCGGA 1 cut(s) 1305
Bsp19I CCATGG 1 cut(s) 1502
BspACI CCGC 6 cut(s) 277, 711, 1523, 1554, 1836, 1839
BspANI GGCC 6 cut(s) 5, 617, 754, 1270, 1778, 1851
BspCNI CTCAG 3 cut(s) 239, 1308, 1525
BspEI TCCGGA 1 cut(s) 1305
BspLI GGNNCC 6 cut(s) 628, 1071, 1170, 1269, 1381, 1599
BspMAI CTGCAG 1 cut(s) 1606
BspMI ACCTGC 1 cut(s) 1609
BspPI GGATC 4 cut(s) 244, 251, 504, 529
BspT107I GGYRCC 1 cut(s) 1597
BsrDI GCAATG 1 cut(s) 286
BsrFI RCCGGY 1 cut(s) 1665
BsrI ACTGG 1 cut(s) 1462
BssAI RCCGGY 1 cut(s) 1665
BssECI CCNNGG 6 cut(s) 310, 974, 1073, 1328, 1383, 1502
BssNI GRCGYC 3 cut(s) 1389, 1630, 1644
BssSI CACGAG 2 cut(s) 293, 1853
BssT1I CCWWGG 3 cut(s) 974, 1073, 1502
Bst2BI CACGAG 2 cut(s) 293, 1853
Bst2UI CCWGG 3 cut(s) 1166, 1329, 1372
Bst4CI ACNGT 7 cut(s) 136, 490, 636, 907, 1017, 1252, 1829
BstACI GRCGYC 3 cut(s) 1389, 1630, 1644
BstC8I GCNNGC 3 cut(s) 7, 1520, 1558
BstDEI CTNAG 5 cut(s) 226, 517, 756, 1295, 1533
BstDSI CCRYGG 1 cut(s) 1502
BstF5I GGATG 4 cut(s) 455, 781, 1415, 1581
BstHHI GCGC 4 cut(s) 162, 1609, 1864, 1887
BstMAI GTCTC 3 cut(s) 804, 920, 1623
BstMCI CGRYCG 1 cut(s) 1616
BstNI CCWGG 3 cut(s) 1166, 1329, 1372
BstNSI RCATGY 2 cut(s) 817, 1125
BstSCI CCNGG 5 cut(s) 123, 1164, 1327, 1370, 1571
BstSFI CTRYAG 1 cut(s) 1602
BstV1I GCAGC 6 cut(s) 24, 440, 1616, 1621, 1866, 1869
BstV2I GAAGAC 2 cut(s) 582, 1384
BstX2I RGATCY 2 cut(s) 236, 521
BstYI RGATCY 2 cut(s) 236, 521
Bsu36I CCTNAGG 1 cut(s) 756
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 6 cut(s) 5, 617, 754, 1270, 1778, 1851
BtgI CCRYGG 1 cut(s) 1502
BtrI CACGTC 1 cut(s) 1437
BtsCI GGATG 4 cut(s) 455, 781, 1415, 1581
BtsIMutI CAGTG 1 cut(s) 138
BveI ACCTGC 1 cut(s) 1609
Cac8I GCNNGC 3 cut(s) 7, 1520, 1558
CaiI CAGNNNCTG 1 cut(s) 1708
CfoI GCGC 4 cut(s) 162, 1609, 1864, 1887
Cfr10I RCCGGY 1 cut(s) 1665
Cfr13I GGNCC 3 cut(s) 1162, 1268, 1380
CseI GACGC 1 cut(s) 1638
CsiI ACCWGGT 1 cut(s) 1370
Csp6I GTAC 2 cut(s) 421, 1711
CviQI GTAC 2 cut(s) 421, 1711
DdeI CTNAG 5 cut(s) 226, 517, 756, 1295, 1533
DraIII CACNNNGTG 2 cut(s) 1437, 1858
EciI GGCGGA 1 cut(s) 1825
Ecl136II GAGCTC 2 cut(s) 1489, 1675
Eco130I CCWWGG 3 cut(s) 974, 1073, 1502
Eco24I GRGCYC 3 cut(s) 1491, 1677, 1817
Eco47I GGWCC 2 cut(s) 1162, 1380
Eco53kI GAGCTC 2 cut(s) 1489, 1675
Eco57I CTGAAG 2 cut(s) 62, 1685
Eco81I CCTNAGG 1 cut(s) 756
Eco88I CYCGRG 2 cut(s) 1561, 1764
EcoICRI GAGCTC 2 cut(s) 1489, 1675
EcoO109I RGGNCCY 1 cut(s) 1380
EcoRII CCWGG 3 cut(s) 1164, 1327, 1370
EcoT14I CCWWGG 3 cut(s) 974, 1073, 1502
EcoT22I ATGCAT 2 cut(s) 652, 1225
EcoT38I GRGCYC 3 cut(s) 1491, 1677, 1817
ErhI CCWWGG 3 cut(s) 974, 1073, 1502
Esp3I CGTCTC 1 cut(s) 1623
FalI AAGNNNNNCTT 2 cut(s) 1169, 1201
FaqI GGGAC 2 cut(s) 145, 1132
FbaI TGATCA 3 cut(s) 297, 841, 1726
FblI GTMKAC 1 cut(s) 1467
Fnu4HI GCNGC 9 cut(s) 38, 429, 712, 1523, 1605, 1610, 1839, 1880, 1883
FokI GGATG 4 cut(s) 442, 768, 1402, 1588
FriOI GRGCYC 3 cut(s) 1491, 1677, 1817
Fsp4HI GCNGC 9 cut(s) 38, 429, 712, 1523, 1605, 1610, 1839, 1880, 1883
FspBI CTAG 4 cut(s) 728, 849, 1074, 1581
FspI TGCGCA 1 cut(s) 1886
GlaI GCGC 4 cut(s) 161, 1608, 1863, 1886
GluI GCNGC 9 cut(s) 38, 429, 712, 1523, 1605, 1610, 1839, 1880, 1883
GsuI CTGGAG 2 cut(s) 73, 1479
HaeIII GGCC 6 cut(s) 5, 617, 754, 1270, 1778, 1851
HapII CCGG 5 cut(s) 125, 1306, 1572, 1633, 1666
HgaI GACGC 1 cut(s) 1638
HhaI GCGC 4 cut(s) 162, 1609, 1864, 1887
Hin1I GRCGYC 3 cut(s) 1389, 1630, 1644
Hin6I GCGC 4 cut(s) 160, 1607, 1862, 1885
HinP1I GCGC 4 cut(s) 160, 1607, 1862, 1885
HincII GTYRAC 3 cut(s) 274, 1468, 1905
HindII GTYRAC 3 cut(s) 274, 1468, 1905
HindIII AAGCTT 2 cut(s) 824, 1243
HinfI GANTC 4 cut(s) 259, 1333, 1461, 1591
HpaII CCGG 5 cut(s) 125, 1306, 1572, 1633, 1666
HphI GGTGA 4 cut(s) 1010, 1240, 1543, 1600
Hpy166II GTNNAC 5 cut(s) 274, 734, 1162, 1468, 1905
Hpy8I GTNNAC 5 cut(s) 274, 734, 1162, 1468, 1905
Hpy99I CGWCG 6 cut(s) 1438, 1469, 1472, 1646, 1683, 1799
HpyAV CCTTC 6 cut(s) 1147, 1277, 1466, 1558, 1632, 1704
HpyCH4III ACNGT 7 cut(s) 136, 490, 636, 907, 1017, 1252, 1829
HpyCH4IV ACGT 4 cut(s) 1389, 1436, 1644, 1797
HpyF3I CTNAG 5 cut(s) 226, 517, 756, 1295, 1533
HpySE526I ACGT 4 cut(s) 1389, 1436, 1644, 1797
Hsp92I GRCGYC 3 cut(s) 1389, 1630, 1644
HspAI GCGC 4 cut(s) 160, 1607, 1862, 1885
Kpn2I TCCGGA 1 cut(s) 1305
Ksp22I TGATCA 3 cut(s) 297, 841, 1726
LmnI GCTCC 5 cut(s) 662, 1440, 1638, 1680, 1720
Lsp1109I GCAGC 6 cut(s) 24, 440, 1616, 1621, 1866, 1869
LweI GCATC 5 cut(s) 403, 464, 631, 637, 1417
MabI ACCWGGT 1 cut(s) 1370
MaeI CTAG 4 cut(s) 728, 849, 1074, 1581
MaeII ACGT 4 cut(s) 1389, 1436, 1644, 1797
MaeIII GTNAC 2 cut(s) 136, 1843
MboII GAAGA 8 cut(s) 62, 298, 517, 582, 785, 1384, 1436, 1922
MflI RGATCY 2 cut(s) 236, 521
MhlI GDGCHC 5 cut(s) 294, 659, 1491, 1677, 1817
MluCI AATT 9 cut(s) 110, 301, 367, 702, 804, 891, 1236, 1299, 1311
MlyI GAGTC 2 cut(s) 1470, 1600
MmeI TCCRAC 7 cut(s) 37, 214, 369, 676, 684, 926, 1702
Mph1103I ATGCAT 2 cut(s) 652, 1225
MroI TCCGGA 1 cut(s) 1305
MroXI GAANNNNTTC 2 cut(s) 1184, 1693
MseI TTAA 2 cut(s) 402, 1479
MslI CAYNNNNRTG 1 cut(s) 1425
MspA1I CMGCKG 1 cut(s) 1532
MspI CCGG 5 cut(s) 125, 1306, 1572, 1633, 1666
MspR9I CCNGG 5 cut(s) 125, 1166, 1329, 1372, 1573
Mva1269I GAATGC 1 cut(s) 1369
MvaI CCWGG 3 cut(s) 1166, 1329, 1372
NciI CCSGG 2 cut(s) 125, 1573
NcoI CCATGG 1 cut(s) 1502
NlaIV GGNNCC 6 cut(s) 628, 1071, 1170, 1269, 1381, 1599
NmuCI GTSAC 1 cut(s) 136
NsbI TGCGCA 1 cut(s) 1886
NsiI ATGCAT 2 cut(s) 652, 1225
NspI RCATGY 2 cut(s) 817, 1125
PaeR7I CTCGAG 2 cut(s) 1561, 1764
PaqCI CACCTGC 1 cut(s) 1609
PctI GAATGC 1 cut(s) 1369
PdmI GAANNNNTTC 2 cut(s) 1184, 1693
PfeI GAWTC 2 cut(s) 259, 1333
PflMI CCANNNNNTGG 2 cut(s) 1437, 1858
PfoI TCCNGGA 1 cut(s) 1571
PkrI GCNGC 9 cut(s) 39, 430, 713, 1524, 1606, 1611, 1840, 1881, 1884
Ple19I CGATCG 1 cut(s) 1616
PleI GAGTC 2 cut(s) 1469, 1599
PpsI GAGTC 2 cut(s) 1469, 1599
PpuMI RGGWCCY 1 cut(s) 1380
PsiI TTATAA 2 cut(s) 1134, 1278
Psp124BI GAGCTC 2 cut(s) 1491, 1677
Psp5II RGGWCCY 1 cut(s) 1380
Psp6I CCWGG 3 cut(s) 1164, 1327, 1370
PspGI CCWGG 3 cut(s) 1164, 1327, 1370
PspN4I GGNNCC 6 cut(s) 628, 1071, 1170, 1269, 1381, 1599
PspPI GGNCC 3 cut(s) 1162, 1268, 1380
PspPPI RGGWCCY 1 cut(s) 1380
PspXI VCTCGAGB 2 cut(s) 1561, 1764
PstI CTGCAG 1 cut(s) 1606
PstNI CAGNNNCTG 1 cut(s) 1708
PsuI RGATCY 2 cut(s) 236, 521
PvuI CGATCG 1 cut(s) 1616
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 2 cut(s) 422, 1712
RsaNI GTAC 2 cut(s) 421, 1711
RseI CAYNNNNRTG 1 cut(s) 1425
SacI GAGCTC 2 cut(s) 1491, 1677
SalI GTCGAC 1 cut(s) 1466
SaqAI TTAA 2 cut(s) 402, 1479
SatI GCNGC 9 cut(s) 38, 429, 712, 1523, 1605, 1610, 1839, 1880, 1883
Sau96I GGNCC 3 cut(s) 1162, 1268, 1380
SchI GAGTC 2 cut(s) 1470, 1600
ScrFI CCNGG 5 cut(s) 125, 1166, 1329, 1372, 1573
SduI GDGCHC 5 cut(s) 294, 659, 1491, 1677, 1817
SexAI ACCWGGT 1 cut(s) 1370
SfaNI GCATC 5 cut(s) 403, 464, 631, 637, 1417
SfcI CTRYAG 1 cut(s) 1602
Sfr274I CTCGAG 2 cut(s) 1561, 1764
SgrDI CGTCGACG 1 cut(s) 1466
SinI GGWCC 2 cut(s) 1162, 1380
SlaI CTCGAG 2 cut(s) 1561, 1764
SmiMI CAYNNNNRTG 1 cut(s) 1425
SmlI CTYRAG 3 cut(s) 1350, 1561, 1764
SmoI CTYRAG 3 cut(s) 1350, 1561, 1764
Sse9I AATT 9 cut(s) 110, 301, 367, 702, 804, 891, 1236, 1299, 1311
SsiI CCGC 6 cut(s) 277, 711, 1523, 1554, 1836, 1839
SspMI CTAG 4 cut(s) 728, 849, 1074, 1581
SstI GAGCTC 2 cut(s) 1491, 1677
StyD4I CCNGG 5 cut(s) 123, 1164, 1327, 1370, 1571
StyI CCWWGG 3 cut(s) 974, 1073, 1502
TaaI ACNGT 7 cut(s) 136, 490, 636, 907, 1017, 1252, 1829
TaiI ACGT 4 cut(s) 1392, 1439, 1647, 1800
TaqI TCGA 9 cut(s) 246, 528, 1230, 1467, 1491, 1562, 1765, 1794, 1893
TasI AATT 9 cut(s) 110, 301, 367, 702, 804, 891, 1236, 1299, 1311
TatI WGTACW 1 cut(s) 1710
TauI GCSGC 3 cut(s) 714, 1525, 1841
TfiI GAWTC 2 cut(s) 259, 1333
Tru1I TTAA 2 cut(s) 402, 1479
Tru9I TTAA 2 cut(s) 402, 1479
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 6 cut(s) 37, 428, 1604, 1609, 1879, 1882
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 6 cut(s) 695, 734, 994, 1002, 1161, 1437
TspGWI ACGGA 2 cut(s) 259, 1922
TspRI CASTG 1 cut(s) 145
Van91I CCANNNNNTGG 2 cut(s) 1437, 1858
VpaK11BI GGWCC 2 cut(s) 1162, 1380
XapI RAATTY 4 cut(s) 110, 367, 702, 1299
XbaI TCTAGA 1 cut(s) 1580
XceI RCATGY 2 cut(s) 817, 1125
XhoI CTCGAG 2 cut(s) 1561, 1764
XmaJI CCTAGG 1 cut(s) 1073
XmiI GTMKAC 1 cut(s) 1467
XmnI GAANNNNTTC 2 cut(s) 1184, 1693
XspI CTAG 4 cut(s) 728, 849, 1074, 1581
ZraI GACGTC 2 cut(s) 1390, 1645
Zsp2I ATGCAT 2 cut(s) 652, 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.