Rmu_sc0003055.1_g000014

Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003055.1
OG0006547
Physical Location & Seq
Forward (+)
50661 .. 52453
1793 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
atgacatcgccggagcaaggatttccgatcgggtctgaatacctcaaacattgccaccttacagcgctgtcgtggaggagaatcaccggtagaggccaccatagggaggagcagaacaaggtggcgagttggtctggaaagtttcaccagcagaggtcgccggagattgtcgataaagccgggtcgagtcggccagatcctggtctaggtacggctttcatggcgggatctttgtctcttctcctcagacggctttacctttccctctacaactggaccctcttcatcggatggtctcaggtgctgtatcttgctgtgaagacacttcgggaatcgggccccgagcaggtttacaatgccctcgaccgcccacttcagtttgctcaatccgccgcgtttttggagattcttcatggcttagtgggtttggtgagatctcctataacagccacactgccacagattagttcgaggttgtttctggtttgggggatcctgtggagctttcctgaggttggtatgaagattcagagttgtagtcacatttag

Protein Analysis

182

Amino Acids

20.47

Weight (kDa)

9.64

Isoelectric Point (pI)

63.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTPLA PF04387 129 - 175 6.3e-13 Protein tyrosine phosphatase-like protein, PTPLA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 337
AccB7I CCANNNNNTGG 1 cut(s) 200
AccII CGCG 1 cut(s) 395
AciI CCGC 4 cut(s) 224, 367, 390, 393
AclWI GGATC 4 cut(s) 191, 235, 487, 500
AcoI YGGCCR 1 cut(s) 191
AcuI CTGAAG 1 cut(s) 359
AfaI GTAC 1 cut(s) 211
AfeI AGCGCT 1 cut(s) 66
AfiI CCNNNNNNNGG 7 cut(s) 17, 103, 106, 200, 206, 346, 515
AgeI ACCGGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 199
AluBI AGCT 1 cut(s) 504
AluI AGCT 1 cut(s) 504
Alw26I GTCTC 2 cut(s) 240, 300
AlwI GGATC 4 cut(s) 191, 235, 487, 500
AlwNI CAGNNNCTG 2 cut(s) 200, 304
Ama87I CYCGRG 1 cut(s) 341
Aor51HI AGCGCT 1 cut(s) 66
AoxI GGCC 3 cut(s) 94, 191, 337
ApaI GGGCCC 1 cut(s) 341
AsiGI ACCGGT 1 cut(s) 86
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 3 cut(s) 276, 337, 338
AsuC2I CCSGG 1 cut(s) 181
AsuHPI GGTGA 3 cut(s) 76, 137, 442
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 1 cut(s) 276
AxyI CCTNAGG 1 cut(s) 510
BaeGI GKGCMC 1 cut(s) 341
BamHI GGATCC 1 cut(s) 492
BanII GRGCYC 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 326
BccI CCATC 1 cut(s) 285
BceAI ACGGC 2 cut(s) 228, 266
BciT130I CCWGG 1 cut(s) 201
BcnI CCSGG 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 240, 300
BfaI CTAG 1 cut(s) 206
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 337
BglII AGATCT 1 cut(s) 434
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
Bme1390I CCNGG 2 cut(s) 181, 201
Bme18I GGWCC 1 cut(s) 276
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 3 cut(s) 276, 337, 338
BmiI GGNNCC 4 cut(s) 278, 339, 340, 494
BmrFI CCNGG 2 cut(s) 181, 201
BpiI GAAGAC 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 181
BsaI GGTCTC 1 cut(s) 300
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 7 cut(s) 17, 103, 106, 200, 206, 346, 515
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 278
Bse21I CCTNAGG 1 cut(s) 510
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 296
BseLI CCNNNNNNNGG 7 cut(s) 17, 103, 106, 200, 206, 346, 515
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 3 cut(s) 259, 311, 501
BseNI ACTGG 1 cut(s) 278
BseRI GAGGAG 3 cut(s) 91, 122, 233
BseSI GKGCMC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 395
Bsh1285I CGRYCG 2 cut(s) 30, 367
BshFI GGCC 3 cut(s) 96, 193, 339
BshTI ACCGGT 1 cut(s) 86
BsiEI CGRYCG 2 cut(s) 30, 367
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 4 cut(s) 11, 87, 161, 180
BslI CCNNNNNNNGG 7 cut(s) 17, 103, 106, 200, 206, 346, 515
BsmAI GTCTC 2 cut(s) 240, 300
BsnI GGCC 3 cut(s) 96, 193, 339
Bso31I GGTCTC 1 cut(s) 300
BsoBI CYCGRG 1 cut(s) 341
Bsp120I GGGCCC 1 cut(s) 337
Bsp1286I GDGCHC 1 cut(s) 341
Bsp143I GATC 5 cut(s) 27, 196, 227, 434, 492
BspACI CCGC 4 cut(s) 224, 367, 390, 393
BspANI GGCC 3 cut(s) 96, 193, 339
BspCNI CTCAG 3 cut(s) 258, 310, 502
BspFNI CGCG 1 cut(s) 395
BspLI GGNNCC 4 cut(s) 278, 339, 340, 494
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 4 cut(s) 191, 235, 487, 500
BspTNI GGTCTC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 49
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 278
BssAI RCCGGY 1 cut(s) 86
BssMI GATC 5 cut(s) 27, 196, 227, 434, 492
Bst2UI CCWGG 1 cut(s) 201
Bst6I CTCTTC 2 cut(s) 243, 287
BstDEI CTNAG 4 cut(s) 245, 297, 418, 510
BstENI CCTNNNNNAGG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 296
BstFNI CGCG 1 cut(s) 395
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 5 cut(s) 30, 199, 230, 437, 495
BstMAI GTCTC 2 cut(s) 240, 300
BstMBI GATC 5 cut(s) 27, 196, 227, 434, 492
BstMCI CGRYCG 2 cut(s) 30, 367
BstMWI GCNNNNNNNGC 3 cut(s) 157, 221, 389
BstNI CCWGG 1 cut(s) 201
BstSCI CCNGG 2 cut(s) 179, 199
BstSLI GKGCMC 1 cut(s) 341
BstUI CGCG 1 cut(s) 395
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 4 cut(s) 196, 227, 434, 492
BstYI RGATCY 4 cut(s) 196, 227, 434, 492
Bsu36I CCTNAGG 1 cut(s) 510
BsuRI GGCC 3 cut(s) 96, 193, 339
BtsCI GGATG 1 cut(s) 296
BtsI GCAGTG 1 cut(s) 452
BtsIMutI CAGTG 1 cut(s) 452
BveI ACCTGC 1 cut(s) 337
CaiI CAGNNNCTG 2 cut(s) 200, 304
CfoI GCGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 3 cut(s) 276, 337, 338
Csp6I GTAC 1 cut(s) 210
CspAI ACCGGT 1 cut(s) 86
CviAII CATG 2 cut(s) 220, 413
CviJI RGCY 9 cut(s) 96, 179, 193, 215, 253, 339, 417, 449, 504
CviKI_1 RGCY 9 cut(s) 96, 179, 193, 215, 253, 339, 417, 449, 504
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 4 cut(s) 245, 297, 418, 510
DpnI GATC 5 cut(s) 29, 198, 229, 436, 494
DpnII GATC 5 cut(s) 27, 196, 227, 434, 492
EaeI YGGCCR 1 cut(s) 191
Eam1104I CTCTTC 2 cut(s) 243, 287
EarI CTCTTC 2 cut(s) 243, 287
EciI GGCGGA 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 276
Eco47III AGCGCT 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 359
Eco81I CCTNAGG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 341
EcoNI CCTNNNNNAGG 1 cut(s) 204
EcoO109I RGGNCCY 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 199
EcoT38I GRGCYC 1 cut(s) 341
FaeI CATG 2 cut(s) 223, 416
FaiI YATR 5 cut(s) 102, 221, 414, 443, 521
FatI CATG 2 cut(s) 219, 412
FauI CCCGC 1 cut(s) 217
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 1 cut(s) 303
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 393
FspBI CTAG 1 cut(s) 206
GlaI GCGC 1 cut(s) 66
GluI GCNGC 1 cut(s) 393
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 3 cut(s) 96, 193, 339
HapII CCGG 4 cut(s) 11, 87, 161, 180
HhaI GCGC 1 cut(s) 67
Hin1II CATG 2 cut(s) 223, 416
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HinfI GANTC 5 cut(s) 81, 187, 332, 406, 526
HpaII CCGG 4 cut(s) 11, 87, 161, 180
HphI GGTGA 3 cut(s) 76, 137, 442
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 5 cut(s) 27, 37, 248, 290, 531
Hpy188III TCNNGA 3 cut(s) 135, 329, 509
Hpy8I GTNNAC 1 cut(s) 352
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 221, 389
HpyF3I CTNAG 4 cut(s) 245, 297, 418, 510
Hsp92II CATG 2 cut(s) 223, 416
HspAI GCGC 1 cut(s) 65
Kzo9I GATC 5 cut(s) 27, 196, 227, 434, 492
LmnI GCTCC 3 cut(s) 13, 109, 501
MaeI CTAG 1 cut(s) 206
MaeIII GTNAC 1 cut(s) 539
MalI GATC 5 cut(s) 29, 198, 229, 436, 494
MboI GATC 5 cut(s) 27, 196, 227, 434, 492
MboII GAAGA 5 cut(s) 230, 274, 331, 401, 535
MflI RGATCY 4 cut(s) 196, 227, 434, 492
MhlI GDGCHC 1 cut(s) 341
MlyI GAGTC 1 cut(s) 196
MspI CCGG 4 cut(s) 11, 87, 161, 180
MspR9I CCNGG 2 cut(s) 181, 201
MvaI CCWGG 1 cut(s) 201
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 3 cut(s) 157, 221, 389
NciI CCSGG 1 cut(s) 181
NdeII GATC 5 cut(s) 27, 196, 227, 434, 492
NlaIII CATG 2 cut(s) 223, 416
NlaIV GGNNCC 4 cut(s) 278, 339, 340, 494
NmuCI GTSAC 1 cut(s) 539
PfeI GAWTC 4 cut(s) 81, 332, 406, 526
PflMI CCANNNNNTGG 1 cut(s) 200
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 1 cut(s) 394
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 4 cut(s) 278, 339, 340, 494
PspOMI GGGCCC 1 cut(s) 337
PspPI GGNCC 3 cut(s) 276, 337, 338
PstNI CAGNNNCTG 2 cut(s) 200, 304
PsuI RGATCY 4 cut(s) 196, 227, 434, 492
PvuI CGATCG 1 cut(s) 30
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 5 cut(s) 27, 196, 227, 434, 492
Sau96I GGNCC 3 cut(s) 276, 337, 338
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 2 cut(s) 181, 201
SduI GDGCHC 1 cut(s) 341
SinI GGWCC 1 cut(s) 276
SsiI CCGC 4 cut(s) 224, 367, 390, 393
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 179, 199
TaqI TCGA 4 cut(s) 171, 185, 363, 470
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 4 cut(s) 81, 332, 406, 526
TscAI CASTG 1 cut(s) 459
TseFI GTSAC 1 cut(s) 539
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 4 cut(s) 208, 274, 401, 536
TspRI CASTG 1 cut(s) 459
Van91I CCANNNNNTGG 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 276
XagI CCTNNNNNAGG 1 cut(s) 204
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.