Rmu_sc0003333.1_g000009

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003333.1
OG0006617
Physical Location & Seq
Reverse (-)
22804 .. 32529
9726 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5838 bp
atgaccaagaagctcacacaatcggaaccacaactcgccatggagctcgaccttgactcgttcctcaactcacacctctcctcctcctcctccgactccgaccccgacctcacctccgtcccgcgccgcacgatcgacgaaatcctcaacgacaccgattcatccaggtcagcatccccctcctcttccccgcgtagttcgaccttatctcagttcctcgcgtccgatcccaaaccgccacagagcgacgccgtttcggtcgccagcggcagaaccgagggagcggggaggttgagtcaattggaggagaaggcggtccggccgggtttgaatttgtacagccgggctaggtccggggatttcccggacgattcggtcgggagagtttcgaggccgtcgccgtggctgttgggaggtgtgaggacgaatgcgaagcccggggccgcgctcgcggcggctgcggcggcttcgaggtcgatgccgacgccccacgccgcggcgatcaagtcgaggaggagcgccgggagcgggagtttccagaaagctgtggaggctacggaattgggcgagagatcggagggagggtccaattttaacggggatagcaatgcggttggttccgaaacgacgccgtcgaattcgaatgaagtagaggaggattttggtggtgcgttggggaggaaagatgaggttttggagagggaggtagagcaggtgagtcaggttgatgaagttagtgctggcaatgctggtgaagaattggatgagaattcaacaaatttagatgccagtgatgttagagataatgaattggatgagaatgttcgggtttcggaggaaggtccaccagaggaagaagatttagatgaaaatagtccttcttcgaaacatagagatgttgaggatgagcagcatcttcgtggtattgatgataatgatgaggttggtgataatgataatgataaagatggtgatgatcattttgatgatgatgatgatgcattgggaacttcaatcactcaacttgtggaggagagaatcgagcaactggagaacaggaggattagtaaaaaagcggaaaagaaatcaagaaagccacttgaaatcgctgaagagctggagaagaaacaagcttcaactgctttggattgggaagagggtgctgctgctcagcctatgagacttgagggtgttcggagagggtccaccacgttgggatacttcaatgttgatgctaaaaataccattacgcggaccctttcttctccggctttgaggcgtgatcatggtacgccgcaagtcctgggagttcacagtaattacattgcaataggaatgtcaagaggggttgtccttgttgtccccagtaaatactctccttataatgctgacaacatggatgcaaagctgatatttcttggattgcaaggagagcggtcttatgctgcggtgacttctatatccttcaatcagcaaggggatctactcttagctggatacgccgatggtcacattacagtttgggatgttcagagggcatccgctgccaaagtaattaccggagaacatacagctccagttgtacatactttttttttggggcatgattctcaggtcacacgtaattttaaggcagttactggggatagtaagggtctggtactgttacattccttctctgtggttcccctgcttaatagattctccatcaaaacacagtgccttctagatggacaaaaaacaggcacgacgctgtcagcttcaccgctcctcattgatgaattgagtggaagctcttcactttcctctcagggaaatgcgatgggttcaggcagcagtattggtggtatgatgggtggtgtggttgggggggatgcaggttggaaactcttcaatgaaggatcctctttgtttgaagaaggtgtggtcgtgtttgtcacccatcaaaatgctttggtggtaagactaactcccactttgcatgtctatgcacaactttctaaaccagatggggttcgggagggttccatgccttgtactgcttggaaatgcacaactcaattgcataattcacctacgaatagtgagaatacgcctactgaagcagttgaaagagtttcattacttgcaattgcttgggatcggaaagttcaggtagcaaagttggtcaaatcagagctgaaagtatatggtaaatggtctcttgaaagtgcagccataggtgtggcttggttggatgatcaggtagcaaagttggtcaaatcagagctgaaagtatatggtaaatggtctcttgaaagtgcagccataggtgtggcttggttggatgatcagatgctggtggttctcacgataactggacaactttgtttgtttgcaaaggatggaactgtaattcaccaaacaagtttttctctagatggttttagaggggatgatctcatttcttatcatactcacttcatcaatgtatttggaaaccctgaaaaagcttataacaattgcatagctgtaagaggagcttctgtatatgtacttggacctactcatcttattgtttcccggcttctcccatggaaggaacggattcaggttttacggagagctggtgactggatgggttctttgaacatggcaatgacaatttatgatggccaagctcatggtgttgttgaccttcctagaactctggatgctgtacaggaggtcataatgccctacctggttgagttgcttttgtcatatgtagaggaagttttttcttatatttcagtagcattctgcaaccaaattgaaaaaatggatcaagttgatgacttaaacagacaaagtagtactgtgcacactgaaattaaagagcaatacacccgtgttggtggggttgcagttgaattctgtgtccatatcaagaggactgacatactttttgatgagatcttcttgaagtttgtagctgttcaacagagagacacatttttggaacttttggagccttatatattgaaggacatgcttggttctcttccacctgagattatgcaagctctggtagaacattacagccacaaagggtggttacagcgtgttgagcaatgtgtccttcacatggatatttcttcattggatttcaatcaggttgttaggttatgccgcgagcatggattatatagtgccctggtgtatcttttcaacaaaggattgaatgatttcaggtcaccattagaggagctgttggtggtcttgcgaaatagtcaaaaagaaggtgctatggcccttggatacaggatgctagtttatttgaagtactgcttttcaggccttgcttttccaccaggacaaggaaaaattcctccttcacgcttgccatctctcagaacagaacttttgcagtttttgttggagggttctgatgcccctaattcaagagcactctcaagcgaattcccaggaggagaatatctcaacctttattatctcttagaactagatactgaagctactttagatgttctgagatgtgctttcacagaagttgaaatctcgaaatgggacttctcttctcaaaattcagctgatgcagacacagagctgcagtatgggaataattcaacggctcaaagtcaggattcattggtccagaatacaattgatactcttattcacattattagtaaggacattccccagaaggatggatctgctagcagtgttgactctggatcagttgtagagtggccttcaaagaaagatattgatcatctattcgagtttattgcatattatgttgcatgtggaaaagctaatgtctcgaagactgtactttctcagattttggagtatttgacatcagagaataactttccaacgtctgtttctggggatagcataatttcaaaaagaagagagaagcaggttcttggccttcttgaggtagtgccggagactgattggaattcatcttctgtgttacagctatgtgagaaagcacaattttaccaggtatgtggcctaattcataccagcagacatcagtatattgctgccctggattgctatatgaaagatgcagccgaacccattcatgcctttgcctttatcaacaaaacattgttacagttgactgacgatgaatgtgctgcttttcaatcagcagttatttctagaattccagagctatttaacttaaacagagagggtgcattcttcttggtcatggaccattttaccagtgcagaaggttctcatatcctatctgaacttcgctcccatccacgaagccttttcctgtacttaaaaacagtgattgaggttcatttatctggcaccctcgacttctcaagtttaaaaaccgacaatcttatgggagtaaaggatcaaacaaaaggagttgaggctttcttggaaagggtctctagtttcccacagcttttgcgagatagtcctattaatgtgactgatgatatgattgagctttatctggagctgttatgccagtttgaatgcaaatcagttctcaaattcctagagacatttgatagctatcgtgtggagcactgtttacgcctctgccagaaatatgcaataatagatgcttcatcattcttgttggagagggttggtgatgttggaagtgcacttttacttacactttccactctcaatgacaaatttatcaagcttgaaactgctgttggaagtctaaatttaagtggggcttcacgtggttctgctagtacagaatgcttgagcaatgctttgaaagtggaggaggtgaatgatatagacagtatattgcatgcatgtataggattgtgccaacggaacacccatcgtctaaatcctgatgaatcagaggcactctggttcaaattgcttgactcgttttgtgagcctttaatggattcgtttagtgctggtacagtatctaaaggagaagatcataatagaatggtaaccaattcattggattcacaagaggatgaaatgaatttcacaatcaagtggagaatcccaaaggctcataaaggtgctgatattttaagaaaattgttttctcggttcatcaaggagattgttgagggcatgataggatatgttcgccttccaactatcatgtcaaagctcctttctgacaatggtagccaggaatttggtgattttaaattcacaatattggggatgcttagcacatatggctttgaaagaagaattctggatactgccaagtccctgatagaagatgatacattctatactatgagcatcctcaagaaaggagcctcacatgggtatgctcctcgcaacccaaaatgttgcatatgcgattgcctccttgacaagaactcttccagctacattcgcattttcaattgtggtcatgcaacacatcttaaatgcgaagtttcagagaatgaaacatcaagtagtagctcgtcctctggatgcccagtttgtatgcccaagaagaaatctcagaggtctaaaaacaaatcggttctagcagaggagagtttagtgaaggtgttttcatcaagaacccatcatagtcatggaacagttgttcacccacatgaaagcacttctgagaatagctatgggattcagcagatatcacgatttgagatgttgaccaatttgcagaaacatcgtggattagtcgagattgaaaacatgcctcaattgaggctcgccccaccagctgtgtaccatgaaaaggttaagcacggacctgttctgtcaccaggagagagcagcagtgatcttgtgagaacagagaaacaaagtaaaatcaagcaactcagggaggtaaaagtaaaaggatctacaactaacaagtaa

Protein Analysis

1945

Amino Acids

214.64

Weight (kDa)

5.26

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 414 - 598 2.9e-23 Vps8 beta-propeller
VPS8_N PF23306 415 - 588 5.6e-07 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 442 - 534 6.8e-06 WDR36/Utp21 first beta-propeller
TPR_Vps8 PF12816 992 - 1175 5.8e-51 Vps8 central TPR
TPR_Vps41 PF23556 1487 - 1555 1.3e-06 Vps41 TPR-like region
TPR_VPS8_2 PF25066 1607 - 1754 2.1e-06 VPS8-like, TPR-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1383, 2496
AarI CACCTGC 1 cut(s) 703
AasI GACNNNNNNGTC 1 cut(s) 374
Acc36I ACCTGC 3 cut(s) 703, 1868, 3901
AccB1I GGYRCC 1 cut(s) 4322
AccBSI CCGCTC 4 cut(s) 284, 528, 1435, 1768
AccII CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1252, 3153
AcoI YGGCCR 2 cut(s) 320, 2653
AcuI CTGAAG 3 cut(s) 1131, 2121, 3513
AcvI CACGTG 1 cut(s) 4721
AcyI GRCGYC 3 cut(s) 249, 485, 629
AfiI CCNNNNNNNGG 9 cut(s) 496, 528, 1251, 2578, 3106, 3338, 3928, 5286, 5713
AflIII ACRYGT 1 cut(s) 1619
AjuI GAANNNNNNNTTGG 4 cut(s) 3856, 3888, 4674, 4706
AloI GAACNNNNNNTCC 2 cut(s) 5544, 5576
Alw21I GWGCWC 5 cut(s) 48, 2844, 3430, 4554, 4636
Alw26I GTCTC 8 cut(s) 1174, 2205, 2295, 2961, 3811, 3935, 4414, 4520
Alw44I GTGCAC 2 cut(s) 2840, 4632
AlwNI CAGNNNCTG 2 cut(s) 1640, 2338
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 9 cut(s) 320, 392, 441, 2653, 3270, 3316, 3734, 3919, 4006
ApaLI GTGCAC 2 cut(s) 2840, 4632
ArsI GACNNNNNNTTYG 2 cut(s) 2909, 2941
AseI ATTAAT 1 cut(s) 4446
Asp700I GAANNNNTTC 5 cut(s) 1795, 1889, 2586, 3206, 4077
AspLEI GCGC 3 cut(s) 126, 448, 521
AsuC2I CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2563
AsuII TTCGAA 2 cut(s) 641, 884
AsuNHI GCTAGC 1 cut(s) 3701
AvaI CYCGRG 1 cut(s) 437
BaeGI GKGCMC 3 cut(s) 2844, 3175, 4636
BalI TGGCCA 1 cut(s) 2655
BamHI GGATCC 1 cut(s) 1901
BanI GGYRCC 1 cut(s) 4322
BanII GRGCYC 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 4721
BbsI GAAGAC 1 cut(s) 3818
Bbv12I GWGCWC 5 cut(s) 48, 2844, 3430, 4554, 4636
BceAI ACGGC 5 cut(s) 236, 379, 385, 616, 3627
BciVI GTATCC 4 cut(s) 1211, 1490, 3272, 5209
BclI TGATCA 5 cut(s) 976, 1282, 2239, 2329, 3754
BcnI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2563
BcoDI GTCTC 8 cut(s) 1174, 2205, 2295, 2961, 3811, 3935, 4414, 4520
BfmI CTRYAG 1 cut(s) 3590
BfoI RGCGCY 1 cut(s) 522
BfuAI ACCTGC 3 cut(s) 703, 1868, 3901
BfuI GTATCC 4 cut(s) 1211, 1490, 3272, 5209
BglII AGATCT 1 cut(s) 2934
BlpI GCTNAGC 2 cut(s) 1170, 5183
BmcAI AGTACT 2 cut(s) 2836, 3305
BmeT110I CYCGRG 1 cut(s) 437
BmrI ACTGGG 3 cut(s) 1359, 1650, 5441
BmtI GCTAGC 1 cut(s) 3705
BmuI ACTGGG 3 cut(s) 1359, 1650, 5441
BpiI GAAGAC 1 cut(s) 3818
BplI GAGNNNNNCTC 2 cut(s) 3444, 3476
BpmI CTGGAG 4 cut(s) 1070, 1139, 1560, 4499
Bpu1102I GCTNAGC 2 cut(s) 1170, 5183
Bpu14I TTCGAA 2 cut(s) 641, 884
BpuEI CTTGAG 6 cut(s) 1205, 3418, 3947, 4321, 4765, 5252
BpuMI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2563
BsaAI YACGTR 2 cut(s) 1622, 4721
BsaBI GATNNNNATC 3 cut(s) 3129, 3753, 4539
BsaHI GRCGYC 3 cut(s) 249, 485, 629
BsaI GGTCTC 3 cut(s) 2205, 2295, 4414
BsaWI WCCGGW 1 cut(s) 1559
Bsc4I CCNNNNNNNGG 9 cut(s) 496, 528, 1251, 2578, 3106, 3338, 3928, 5286, 5713
Bse3DI GCAATG 6 cut(s) 613, 751, 1323, 2643, 3098, 4756
Bse8I GATNNNNATC 3 cut(s) 3129, 3753, 4539
BseJI GATNNNNATC 3 cut(s) 3129, 3753, 4539
BseLI CCNNNNNNNGG 9 cut(s) 496, 528, 1251, 2578, 3106, 3338, 3928, 5286, 5713
BseMI GCAATG 6 cut(s) 613, 751, 1323, 2643, 3098, 4756
BseSI GKGCMC 3 cut(s) 2844, 3175, 4636
BseX3I CGGCCG 1 cut(s) 320
BsgI GTGCAG 3 cut(s) 2232, 2322, 4251
Bsh1236I CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1252, 3153
Bsh1285I CGRYCG 4 cut(s) 135, 261, 323, 378
BshFI GGCC 9 cut(s) 322, 394, 443, 2655, 3272, 3318, 3736, 3921, 4008
BshNI GGYRCC 1 cut(s) 4322
BsiEI CGRYCG 4 cut(s) 135, 261, 323, 378
BsiHKAI GWGCWC 5 cut(s) 48, 2844, 3430, 4554, 4636
BsiHKCI CYCGRG 1 cut(s) 437
BslFI GGGAC 4 cut(s) 104, 1346, 3563, 5212
BslI CCNNNNNNNGG 9 cut(s) 496, 528, 1251, 2578, 3106, 3338, 3928, 5286, 5713
BsmAI GTCTC 8 cut(s) 1174, 2205, 2295, 2961, 3811, 3935, 4414, 4520
BsmFI GGGAC 4 cut(s) 104, 1346, 3563, 5212
BsmI GAATGC 5 cut(s) 433, 2777, 4199, 4505, 4745
BsnI GGCC 9 cut(s) 322, 394, 443, 2655, 3272, 3318, 3736, 3921, 4008
Bso31I GGTCTC 3 cut(s) 2205, 2295, 4414
BsoBI CYCGRG 1 cut(s) 437
Bsp119I TTCGAA 2 cut(s) 641, 884
Bsp1286I GDGCHC 6 cut(s) 48, 2844, 3175, 3430, 4554, 4636
Bsp1407I TGTACA 3 cut(s) 336, 1582, 2698
Bsp1720I GCTNAGC 2 cut(s) 1170, 5183
Bsp19I CCATGG 2 cut(s) 39, 2573
BspANI GGCC 9 cut(s) 322, 394, 443, 2655, 3272, 3318, 3736, 3921, 4008
BspFNI CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1252, 3153
BspMAI CTGCAG 1 cut(s) 3594
BspMI ACCTGC 3 cut(s) 703, 1868, 3901
BspOI GCTAGC 1 cut(s) 3705
BspQI GCTCTTC 2 cut(s) 1107, 1801
BspT104I TTCGAA 2 cut(s) 641, 884
BspT107I GGYRCC 1 cut(s) 4322
BspTNI GGTCTC 3 cut(s) 2205, 2295, 4414
BsrBI CCGCTC 4 cut(s) 284, 528, 1435, 1768
BsrDI GCAATG 6 cut(s) 613, 751, 1323, 2643, 3098, 4756
BsrGI TGTACA 3 cut(s) 336, 1582, 2698
BssNI GRCGYC 3 cut(s) 249, 485, 629
BssT1I CCWWGG 3 cut(s) 39, 2573, 3274
Bst6I CTCTTC 9 cut(s) 190, 1107, 1149, 1801, 1895, 3027, 3562, 3895, 5350
BstACI GRCGYC 3 cut(s) 249, 485, 629
BstAPI GCANNNNNTGC 1 cut(s) 1544
BstAUI TGTACA 3 cut(s) 336, 1582, 2698
BstBAI YACGTR 2 cut(s) 1622, 4721
BstBI TTCGAA 2 cut(s) 641, 884
BstC8I GCNNGC 9 cut(s) 265, 450, 742, 3042, 3155, 3362, 3703, 4797, 5688
BstDSI CCRYGG 4 cut(s) 39, 401, 495, 2573
BstEII GGTNACC 2 cut(s) 3213, 4951
BstENI CCTNNNNNAGG 1 cut(s) 3926
BstFNI CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1252, 3153
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 3 cut(s) 126, 448, 521
BstMAI GTCTC 8 cut(s) 1174, 2205, 2295, 2961, 3811, 3935, 4414, 4520
BstMCI CGRYCG 4 cut(s) 135, 261, 323, 378
BstNSI RCATGY 6 cut(s) 1985, 3013, 3792, 4799, 4803, 5674
BstPI GGTNACC 2 cut(s) 3213, 4951
BstSFI CTRYAG 1 cut(s) 3590
BstSLI GKGCMC 3 cut(s) 2844, 3175, 4636
BstUI CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1252, 3153
BstV2I GAAGAC 1 cut(s) 3818
BstX2I RGATCY 5 cut(s) 1480, 1901, 2934, 3695, 5819
BstXI CCANNNNNNTGG 8 cut(s) 1213, 2224, 2314, 2663, 3692, 4004, 4963, 5150
BstYI RGATCY 5 cut(s) 1480, 1901, 2934, 3695, 5819
BstZI CGGCCG 1 cut(s) 320
BsuI GTATCC 4 cut(s) 1211, 1490, 3272, 5209
BsuRI GGCC 9 cut(s) 322, 394, 443, 2655, 3272, 3318, 3736, 3921, 4008
BtgI CCRYGG 4 cut(s) 39, 401, 495, 2573
BtgZI GCGATG 1 cut(s) 1835
BtsI GCAGTG 2 cut(s) 3712, 5761
BtsIMutI CAGTG 8 cut(s) 796, 1724, 2844, 3712, 4234, 4305, 4552, 5761
BveI ACCTGC 3 cut(s) 703, 1868, 3901
Cac8I GCNNGC 9 cut(s) 265, 450, 742, 3042, 3155, 3362, 3703, 4797, 5688
CaiI CAGNNNCTG 2 cut(s) 1640, 2338
CfoI GCGC 3 cut(s) 126, 448, 521
Cfr42I CCGCGG 1 cut(s) 498
Cfr9I CCCGGG 1 cut(s) 437
CpoI CGGWCCG 1 cut(s) 316
CseI GACGC 5 cut(s) 210, 257, 493, 637, 1759
CsiI ACCWGGT 2 cut(s) 2721, 3996
CspCI CAANNNNNGTGG 2 cut(s) 4410, 4445
CspI CGGWCCG 1 cut(s) 316
DraI TTTAAA 2 cut(s) 4344, 5161
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 2 cut(s) 320, 2653
EagI CGGCCG 1 cut(s) 320
Eam1104I CTCTTC 9 cut(s) 190, 1107, 1149, 1801, 1895, 3027, 3562, 3895, 5350
EarI CTCTTC 9 cut(s) 190, 1107, 1149, 1801, 1895, 3027, 3562, 3895, 5350
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 320
Eco130I CCWWGG 3 cut(s) 39, 2573, 3274
Eco147I AGGCCT 1 cut(s) 3318
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 3 cut(s) 2205, 2295, 4414
Eco32I GATATC 1 cut(s) 5610
Eco52I CGGCCG 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 3 cut(s) 1131, 2121, 3513
Eco72I CACGTG 1 cut(s) 4721
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 2 cut(s) 3213, 4951
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 3926
EcoO65I GGTNACC 2 cut(s) 3213, 4951
EcoRI GAATTC 7 cut(s) 637, 769, 2891, 3440, 3952, 4164, 5208
EcoRV GATATC 1 cut(s) 5610
EcoT14I CCWWGG 3 cut(s) 39, 2573, 3274
EcoT22I ATGCAT 2 cut(s) 1003, 4801
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 39, 2573, 3274
FalI AAGNNNNNCTT 6 cut(s) 2506, 2538, 3293, 3325, 4699, 4731
FaqI GGGAC 4 cut(s) 104, 1346, 3563, 5212
FauI CCCGC 4 cut(s) 129, 198, 277, 521
FauNDI CATATG 3 cut(s) 2743, 5191, 5318
FbaI TGATCA 5 cut(s) 976, 1282, 2239, 2329, 3754
FriOI GRGCYC 1 cut(s) 48
GlaI GCGC 3 cut(s) 125, 447, 520
GsuI CTGGAG 4 cut(s) 1070, 1139, 1560, 4499
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 9 cut(s) 322, 394, 443, 2655, 3272, 3318, 3736, 3921, 4008
HgaI GACGC 5 cut(s) 210, 257, 493, 637, 1759
HhaI GCGC 3 cut(s) 126, 448, 521
Hin1I GRCGYC 3 cut(s) 249, 485, 629
Hin6I GCGC 3 cut(s) 124, 446, 519
HinP1I GCGC 3 cut(s) 124, 446, 519
HincII GTYRAC 4 cut(s) 2674, 3712, 4119, 5628
HindII GTYRAC 4 cut(s) 2674, 3712, 4119, 5628
HindIII AAGCTT 3 cut(s) 1131, 2490, 4676
Hpy99I CGWCG 7 cut(s) 140, 251, 400, 487, 631, 637, 1753
HpyCH4IV ACGT 4 cut(s) 1211, 1621, 3866, 4720
HpySE526I ACGT 4 cut(s) 1211, 1621, 3866, 4720
Hsp92I GRCGYC 3 cut(s) 249, 485, 629
HspAI GCGC 3 cut(s) 124, 446, 519
Ksp22I TGATCA 5 cut(s) 976, 1282, 2239, 2329, 3754
KspI CCGCGG 1 cut(s) 498
LguI GCTCTTC 2 cut(s) 1107, 1801
MabI ACCWGGT 2 cut(s) 2721, 3996
MaeII ACGT 4 cut(s) 1211, 1621, 3866, 4720
MbiI CCGCTC 4 cut(s) 284, 528, 1435, 1768
MfeI CAATTG 7 cut(s) 299, 2060, 2130, 2500, 3645, 5368, 5678
MflI RGATCY 5 cut(s) 1480, 1901, 2934, 3695, 5819
MhlI GDGCHC 6 cut(s) 48, 2844, 3175, 3430, 4554, 4636
MlsI TGGCCA 1 cut(s) 2655
MluNI TGGCCA 1 cut(s) 2655
MlyI GAGTC 6 cut(s) 50, 89, 304, 727, 3707, 4871
Mox20I TGGCCA 1 cut(s) 2655
Mph1103I ATGCAT 2 cut(s) 1003, 4801
MroXI GAANNNNTTC 5 cut(s) 1795, 1889, 2586, 3206, 4077
MscI TGGCCA 1 cut(s) 2655
Msp20I TGGCCA 1 cut(s) 2655
MspA1I CMGCKG 5 cut(s) 267, 497, 1544, 3572, 5699
MunI CAATTG 7 cut(s) 299, 2060, 2130, 2500, 3645, 5368, 5678
Mva1269I GAATGC 5 cut(s) 433, 2777, 4199, 4505, 4745
MvnI CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1252, 3153
NciI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2563
NcoI CCATGG 2 cut(s) 39, 2573
NdeI CATATG 3 cut(s) 2743, 5191, 5318
NheI GCTAGC 1 cut(s) 3701
NmuCI GTSAC 8 cut(s) 1450, 1508, 1615, 1936, 2609, 3213, 4450, 5736
NsiI ATGCAT 2 cut(s) 1003, 4801
NspI RCATGY 6 cut(s) 1985, 3013, 3792, 4799, 4803, 5674
NspV TTCGAA 2 cut(s) 641, 884
PaeI GCATGC 1 cut(s) 4799
PaqCI CACCTGC 1 cut(s) 703
PceI AGGCCT 1 cut(s) 3318
PciSI GCTCTTC 2 cut(s) 1107, 1801
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PctI GAATGC 5 cut(s) 433, 2777, 4199, 4505, 4745
PdmI GAANNNNTTC 5 cut(s) 1795, 1889, 2586, 3206, 4077
PflFI GACNNNGTC 2 cut(s) 631, 1753
PfoI TCCNGGA 1 cut(s) 363
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 6 cut(s) 50, 89, 303, 726, 3707, 4871
PmaCI CACGTG 1 cut(s) 4721
PmlI CACGTG 1 cut(s) 4721
PpsI GAGTC 6 cut(s) 50, 89, 303, 726, 3707, 4871
Ppu21I YACGTR 2 cut(s) 1622, 4721
PshBI ATTAAT 1 cut(s) 4446
PsiI TTATAA 2 cut(s) 1383, 2496
Psp124BI GAGCTC 1 cut(s) 48
PspCI CACGTG 1 cut(s) 4721
PspEI GGTNACC 2 cut(s) 3213, 4951
PstI CTGCAG 1 cut(s) 3594
PstNI CAGNNNCTG 2 cut(s) 1640, 2338
PsuI RGATCY 5 cut(s) 1480, 1901, 2934, 3695, 5819
PsyI GACNNNGTC 2 cut(s) 631, 1753
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 2 cut(s) 3572, 5699
Rsr2I CGGWCCG 1 cut(s) 316
RsrII CGGWCCG 1 cut(s) 316
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 498
SapI GCTCTTC 2 cut(s) 1107, 1801
ScaI AGTACT 2 cut(s) 2836, 3305
SchI GAGTC 6 cut(s) 50, 89, 304, 727, 3707, 4871
SduI GDGCHC 6 cut(s) 48, 2844, 3175, 3430, 4554, 4636
SexAI ACCWGGT 2 cut(s) 2721, 3996
SfcI CTRYAG 1 cut(s) 3590
Sfr303I CCGCGG 1 cut(s) 498
SfuI TTCGAA 2 cut(s) 641, 884
SgrBI CCGCGG 1 cut(s) 498
SmaI CCCGGG 1 cut(s) 439
SmlI CTYRAG 6 cut(s) 1184, 3433, 3926, 4336, 4744, 5267
SmoI CTYRAG 6 cut(s) 1184, 3433, 3926, 4336, 4744, 5267
SphI GCATGC 1 cut(s) 4799
SseBI AGGCCT 1 cut(s) 3318
SspI AATATT 1 cut(s) 5172
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 3318
StyI CCWWGG 3 cut(s) 39, 2573, 3274
TaiI ACGT 4 cut(s) 1214, 1624, 3869, 4723
TaqII GACCGA 2 cut(s) 247, 364
TscAI CASTG 8 cut(s) 796, 1724, 2851, 3712, 4234, 4305, 4559, 5761
TseFI GTSAC 8 cut(s) 1450, 1508, 1615, 1936, 2609, 3213, 4450, 5736
Tsp45I GTSAC 8 cut(s) 1450, 1508, 1615, 1936, 2609, 3213, 4450, 5736
TspGWI ACGGA 6 cut(s) 106, 572, 2599, 2614, 4834, 5739
TspMI CCCGGG 1 cut(s) 437
TspRI CASTG 8 cut(s) 796, 1724, 2851, 3712, 4234, 4305, 4559, 5761
Tth111I GACNNNGTC 2 cut(s) 631, 1753
VneI GTGCAC 2 cut(s) 2840, 4632
VspI ATTAAT 1 cut(s) 4446
XagI CCTNNNNNAGG 1 cut(s) 3926
XbaI TCTAGA 3 cut(s) 1726, 2416, 4160
XceI RCATGY 6 cut(s) 1985, 3013, 3792, 4799, 4803, 5674
XcmI CCANNNNNNNNNTGG 1 cut(s) 5546
XmaI CCCGGG 1 cut(s) 437
XmnI GAANNNNTTC 5 cut(s) 1795, 1889, 2586, 3206, 4077
ZrmI AGTACT 2 cut(s) 2836, 3305
Zsp2I ATGCAT 2 cut(s) 1003, 4801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.