Rmu_sc0003628.1_g000028

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003628.1
OG0006958
Physical Location & Seq
Forward (+)
75679 .. 79845
4167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003628.1_g000028.1.cds

Sequence Viewer

Length: 834 bp
atgaggacaatcggcgacgaaatcggtgcgaaactggtcacccatggcgatccttgtgcggagatagtaattgagcagttcctggtcagtgggaaggaacctgacccctactgggagtttgttggagagaattgccatgattgcagaaatggaaagaatttcagagagaaaaaagaactagggtttcgggaagaaaagaatttcagagaaaaaaaagaactagggtttccggaattggagaataggagaattgagagagagaagagtgaagagagcagagtgaagagtggggggactgaaccaaaagatatcgacgccaatacaaaaagaaactctcctgctctcgagcacggtggtaaggtcacccacttcattcacaaagcccttaacactctccccaacaccctcctccacatcatggccgacgagtcatgcatggtcctcaatgtcattcaaaacgatgaatttccgatggtcttggaatcagatatcattcctcacgatattcccattctccaaggaatgaccaaaagcggcgtcaccctcagattcttccataaaaaccgcctcagcatcatccgaggacttgccttgtttttcaatgacgtgtgcttgagagcctttgaggaattgtacattacagctgctcactgccaaaacaagactatagatcatatatccaccggaatagggttacaaccagagccgcaaaactatgtaaggaggatggttctaatcaatgagtactacaatccatttctcattggagctgcgacttttgacacgcctatggaaaccatagaacctcttcctccgcaccccatggatgggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

277

Amino Acids

31.6

Weight (kDa)

5.6

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 418
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 5 cut(s) 59, 534, 565, 707, 815
AclWI GGATC 1 cut(s) 44
AcoI YGGCCR 1 cut(s) 420
AcsI RAATTY 3 cut(s) 157, 199, 464
AcyI GRCGYC 2 cut(s) 315, 537
AfaI GTAC 2 cut(s) 635, 746
AfiI CCNNNNNNNGG 5 cut(s) 112, 418, 690, 827, 828
AflIII ACRYGT 1 cut(s) 606
AgsI TTSAA 2 cut(s) 455, 601
AjiI CACGTC 1 cut(s) 607
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 2 cut(s) 644, 770
AluI AGCT 2 cut(s) 644, 770
Alw21I GWGCWC 1 cut(s) 351
AlwI GGATC 1 cut(s) 44
AlwNI CAGNNNCTG 1 cut(s) 82
Ama87I CYCGRG 1 cut(s) 344
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 1 cut(s) 420
ApeKI GCWGC 2 cut(s) 644, 770
ApoI RAATTY 3 cut(s) 157, 199, 464
ArsI GACNNNNNNTTYG 2 cut(s) 522, 554
Asp700I GAANNNNTTC 1 cut(s) 807
AspS9I GGNCC 1 cut(s) 439
AsuHPI GGTGA 3 cut(s) 31, 355, 532
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 1 cut(s) 439
Bbv12I GWGCWC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 569
BbvI GCAGC 2 cut(s) 631, 757
BccI CCATC 3 cut(s) 466, 721, 821
BcgI CGANNNNNNTGC 4 cut(s) 8, 38, 42, 72
BciT130I CCWGG 1 cut(s) 83
BfaI CTAG 2 cut(s) 179, 221
BfmI CTRYAG 1 cut(s) 666
BisI GCNGC 4 cut(s) 535, 645, 707, 771
BlsI GCNGC 4 cut(s) 536, 646, 708, 772
BmcAI AGTACT 1 cut(s) 746
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 439
BmeT110I CYCGRG 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 439
BmiI GGNNCC 1 cut(s) 99
BmrFI CCNGG 1 cut(s) 83
BmrI ACTGGG 1 cut(s) 121
BmsI GCATC 1 cut(s) 582
BmuI ACTGGG 1 cut(s) 121
Bpu10I CCTNAGC 1 cut(s) 569
BpuEI CTTGAG 1 cut(s) 634
BsaHI GRCGYC 2 cut(s) 315, 537
BsaJI CCNNGG 4 cut(s) 43, 517, 580, 822
BsaWI WCCGGW 2 cut(s) 229, 683
Bsc4I CCNNNNNNNGG 5 cut(s) 112, 418, 690, 827, 828
Bse1I ACTGG 2 cut(s) 39, 116
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 4 cut(s) 43, 517, 580, 822
BseGI GGATG 3 cut(s) 576, 732, 832
BseLI CCNNNNNNNGG 5 cut(s) 112, 418, 690, 827, 828
BseMII CTCAG 2 cut(s) 559, 583
BseNI ACTGG 2 cut(s) 39, 116
BseRI GAGGAG 1 cut(s) 398
BseXI GCAGC 2 cut(s) 631, 757
BshFI GGCC 1 cut(s) 422
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 2 cut(s) 230, 684
BslFI GGGAC 1 cut(s) 307
BslI CCNNNNNNNGG 5 cut(s) 112, 418, 690, 827, 828
BsmFI GGGAC 1 cut(s) 307
BsnI GGCC 1 cut(s) 422
BsoBI CYCGRG 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 351
Bsp13I TCCGGA 1 cut(s) 229
Bsp1407I TGTACA 1 cut(s) 633
Bsp143I GATC 2 cut(s) 49, 670
Bsp19I CCATGG 2 cut(s) 43, 822
BspACI CCGC 5 cut(s) 59, 534, 565, 707, 815
BspANI GGCC 1 cut(s) 422
BspCNI CTCAG 2 cut(s) 558, 582
BspEI TCCGGA 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 99
BspPI GGATC 1 cut(s) 44
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 2 cut(s) 39, 116
BssECI CCNNGG 4 cut(s) 43, 517, 580, 822
BssMI GATC 2 cut(s) 49, 670
BssNI GRCGYC 2 cut(s) 315, 537
BssT1I CCWWGG 3 cut(s) 43, 517, 822
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 353
Bst6I CTCTTC 4 cut(s) 257, 264, 278, 813
BstACI GRCGYC 2 cut(s) 315, 537
BstAUI TGTACA 1 cut(s) 633
BstDEI CTNAG 2 cut(s) 545, 569
BstDSI CCRYGG 2 cut(s) 43, 822
BstEII GGTNACC 2 cut(s) 37, 361
BstF5I GGATG 3 cut(s) 576, 732, 832
BstKTI GATC 2 cut(s) 52, 673
BstMBI GATC 2 cut(s) 49, 670
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstNI CCWGG 1 cut(s) 83
BstPI GGTNACC 2 cut(s) 37, 361
BstSCI CCNGG 1 cut(s) 81
BstSFI CTRYAG 1 cut(s) 666
BstV1I GCAGC 2 cut(s) 631, 757
BsuRI GGCC 1 cut(s) 422
BtgI CCRYGG 2 cut(s) 43, 822
BtrI CACGTC 1 cut(s) 607
BtsCI GGATG 3 cut(s) 576, 732, 832
BtsI GCAGTG 1 cut(s) 649
BtsIMutI CAGTG 2 cut(s) 94, 649
CaiI CAGNNNCTG 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 439
CseI GACGC 2 cut(s) 323, 526
Csp6I GTAC 2 cut(s) 634, 745
CviAII CATG 6 cut(s) 44, 137, 418, 432, 436, 823
CviJI RGCY 6 cut(s) 383, 422, 620, 644, 706, 770
CviKI_1 RGCY 6 cut(s) 383, 422, 620, 644, 706, 770
CviQI GTAC 2 cut(s) 634, 745
DdeI CTNAG 2 cut(s) 545, 569
DpnI GATC 2 cut(s) 51, 672
DpnII GATC 2 cut(s) 49, 670
EaeI YGGCCR 1 cut(s) 420
Eam1104I CTCTTC 4 cut(s) 257, 264, 278, 813
EarI CTCTTC 4 cut(s) 257, 264, 278, 813
Eco130I CCWWGG 3 cut(s) 43, 517, 822
Eco32I GATATC 2 cut(s) 310, 490
Eco47I GGWCC 1 cut(s) 439
Eco88I CYCGRG 1 cut(s) 344
Eco91I GGTNACC 2 cut(s) 37, 361
EcoO65I GGTNACC 2 cut(s) 37, 361
EcoRII CCWGG 1 cut(s) 81
EcoRV GATATC 2 cut(s) 310, 490
EcoT14I CCWWGG 3 cut(s) 43, 517, 822
EcoT22I ATGCAT 1 cut(s) 437
ErhI CCWWGG 3 cut(s) 43, 517, 822
FaeI CATG 6 cut(s) 47, 140, 421, 435, 439, 826
FaqI GGGAC 1 cut(s) 307
FatI CATG 6 cut(s) 43, 136, 417, 431, 435, 822
Fnu4HI GCNGC 4 cut(s) 535, 645, 707, 771
FokI GGATG 2 cut(s) 563, 739
Fsp4HI GCNGC 4 cut(s) 535, 645, 707, 771
FspBI CTAG 2 cut(s) 179, 221
GluI GCNGC 4 cut(s) 535, 645, 707, 771
HaeIII GGCC 1 cut(s) 422
HapII CCGG 2 cut(s) 230, 684
HgaI GACGC 2 cut(s) 323, 526
Hin1I GRCGYC 2 cut(s) 315, 537
Hin1II CATG 6 cut(s) 47, 140, 421, 435, 439, 826
HinfI GANTC 3 cut(s) 428, 482, 549
HpaII CCGG 2 cut(s) 230, 684
HphI GGTGA 3 cut(s) 31, 355, 532
Hpy188I TCNGA 6 cut(s) 164, 206, 471, 487, 548, 581
Hpy188III TCNNGA 4 cut(s) 188, 230, 344, 500
Hpy99I CGWCG 3 cut(s) 20, 317, 428
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4IV ACGT 1 cut(s) 606
HpyCH4V TGCA 2 cut(s) 144, 435
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 2 cut(s) 545, 569
HpySE526I ACGT 1 cut(s) 606
Hsp92I GRCGYC 2 cut(s) 315, 537
Hsp92II CATG 6 cut(s) 47, 140, 421, 435, 439, 826
Kpn2I TCCGGA 1 cut(s) 229
Kzo9I GATC 2 cut(s) 49, 670
LmnI GCTCC 1 cut(s) 767
LpnPI CCDG 9 cut(s) 20, 68, 95, 97, 114, 243, 351, 697, 714
Lsp1109I GCAGC 2 cut(s) 631, 757
LweI GCATC 1 cut(s) 582
MaeI CTAG 2 cut(s) 179, 221
MaeII ACGT 1 cut(s) 606
MaeIII GTNAC 4 cut(s) 37, 361, 538, 693
MalI GATC 2 cut(s) 51, 672
MboI GATC 2 cut(s) 49, 670
MboII GAAGA 6 cut(s) 203, 274, 281, 295, 544, 800
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 8 cut(s) 69, 130, 157, 199, 233, 249, 464, 629
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 103
Mph1103I ATGCAT 1 cut(s) 437
MroI TCCGGA 1 cut(s) 229
MroXI GAANNNNTTC 1 cut(s) 807
MseI TTAA 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 788
MspA1I CMGCKG 1 cut(s) 644
MspI CCGG 2 cut(s) 230, 684
MspR9I CCNGG 1 cut(s) 83
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 141
NcoI CCATGG 2 cut(s) 43, 822
NdeII GATC 2 cut(s) 49, 670
NlaIII CATG 6 cut(s) 47, 140, 421, 435, 439, 826
NlaIV GGNNCC 1 cut(s) 99
NmuCI GTSAC 3 cut(s) 37, 361, 538
NsiI ATGCAT 1 cut(s) 437
PaeR7I CTCGAG 1 cut(s) 344
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 2 cut(s) 482, 549
PflMI CCANNNNNTGG 1 cut(s) 418
PkrI GCNGC 4 cut(s) 536, 646, 708, 772
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 81
PspEI GGTNACC 2 cut(s) 37, 361
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 635, 746
RsaNI GTAC 2 cut(s) 634, 745
RseI CAYNNNNRTG 1 cut(s) 788
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 4 cut(s) 535, 645, 707, 771
Sau3AI GATC 2 cut(s) 49, 670
Sau96I GGNCC 1 cut(s) 439
ScaI AGTACT 1 cut(s) 746
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 1 cut(s) 351
SetI ASST 6 cut(s) 103, 363, 609, 646, 772, 808
SfaNI GCATC 1 cut(s) 582
SfcI CTRYAG 1 cut(s) 666
Sfr274I CTCGAG 1 cut(s) 344
SinI GGWCC 1 cut(s) 439
SlaI CTCGAG 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 788
SmlI CTYRAG 2 cut(s) 344, 613
SmoI CTYRAG 2 cut(s) 344, 613
Sse9I AATT 8 cut(s) 69, 130, 157, 199, 233, 249, 464, 629
SsiI CCGC 5 cut(s) 59, 534, 565, 707, 815
SspMI CTAG 2 cut(s) 179, 221
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 3 cut(s) 43, 517, 822
TaaI ACNGT 1 cut(s) 353
TaiI ACGT 1 cut(s) 609
TaqI TCGA 2 cut(s) 312, 345
TasI AATT 8 cut(s) 69, 130, 157, 199, 233, 249, 464, 629
TatI WGTACW 2 cut(s) 633, 744
TauI GCSGC 2 cut(s) 537, 709
TfiI GAWTC 2 cut(s) 482, 549
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 2 cut(s) 94, 656
TseFI GTSAC 3 cut(s) 37, 361, 538
TseI GCWGC 2 cut(s) 644, 770
Tsp45I GTSAC 3 cut(s) 37, 361, 538
TspDTI ATGAA 2 cut(s) 361, 477
TspRI CASTG 2 cut(s) 94, 656
Van91I CCANNNNNTGG 1 cut(s) 418
VpaK11BI GGWCC 1 cut(s) 439
XapI RAATTY 3 cut(s) 157, 199, 464
XhoI CTCGAG 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 2 cut(s) 179, 221
ZrmI AGTACT 1 cut(s) 746
Zsp2I ATGCAT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.