Rw5G028580
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006958
Physical Location & Seq
Forward (+)
43322661 .. 43323865
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028580.1

Sequence Viewer

Length: 444 bp
ATGGTTGTGATAAATTTAGGACCTATTGAATGTATACCCTTTCAGAGGGAAGTAAATCTGCTAGCTAATCCAGATAAGCACCCAACAAAGGGTGCCTTTGGATGTGCAACACTACTTGATAAAAGCCTACCAATTGAAGAGGGTGATGCTCGCTATATGCCTTCAGAGATCCTGAATGAGAAACATGACGATCTTGACAAGGCTGATATCTTCTCATTGGGAGTTGCTATGTATGAGCTTATTAGAGGTTCACCGTTACCGGAAGAAGGACCTCAAACCTTAAATCTTAAGAAAGGAAAATTGCCACTGCTTCCTGGTCACTCCTTGCAACTTCAGAACTTGCTCAAGGCCATGTTGGATCCAAATCCAGTCTGCAGACCATCAGCTAAAGAGTTGGTTGAAAATCCAATGCATGGGCCTAGGACAACCACGATTGCGAATTGA

Protein Analysis

147

Amino Acids

16.13

Weight (kDa)

5.54

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 19 - 136 3.9e-11 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 413
AccI GTMKAC 1 cut(s) 34
AclWI GGATC 3 cut(s) 163, 353, 366
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 2 cut(s) 147, 317
AfiI CCNNNNNNNGG 5 cut(s) 45, 88, 89, 266, 413
AflII CTTAAG 1 cut(s) 287
AgsI TTSAA 3 cut(s) 29, 137, 401
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 3 cut(s) 65, 238, 386
AluI AGCT 3 cut(s) 65, 238, 386
AlwI GGATC 3 cut(s) 163, 353, 366
AoxI GGCC 2 cut(s) 348, 416
ApoI RAATTY 1 cut(s) 13
AspA2I CCTAGG 1 cut(s) 419
AspS9I GGNCC 3 cut(s) 20, 269, 416
AsuHPI GGTGA 2 cut(s) 155, 243
AsuNHI GCTAGC 1 cut(s) 61
AvaII GGWCC 2 cut(s) 20, 269
AvrII CCTAGG 1 cut(s) 419
BamHI GGATCC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 92
BccI CCATC 1 cut(s) 388
BciT130I CCWGG 1 cut(s) 315
BfaI CTAG 2 cut(s) 62, 420
BfmI CTRYAG 1 cut(s) 373
BfrI CTTAAG 1 cut(s) 287
BlnI CCTAGG 1 cut(s) 419
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 2 cut(s) 20, 269
BmgT120I GGNCC 3 cut(s) 20, 269, 416
BmiI GGNNCC 2 cut(s) 94, 360
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 136
BmtI GCTAGC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 419
BsaWI WCCGGW 1 cut(s) 259
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 88, 89, 266, 413
Bse1I ACTGG 1 cut(s) 368
Bse8I GATNNNNATC 1 cut(s) 363
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 1 cut(s) 107
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 5 cut(s) 45, 88, 89, 266, 413
BseNI ACTGG 1 cut(s) 368
BshFI GGCC 2 cut(s) 350, 418
BshNI GGYRCC 1 cut(s) 92
BsiSI CCGG 1 cut(s) 260
BslI CCNNNNNNNGG 5 cut(s) 45, 88, 89, 266, 413
BsnI GGCC 2 cut(s) 350, 418
Bsp143I GATC 3 cut(s) 168, 190, 358
BspANI GGCC 2 cut(s) 350, 418
BspLI GGNNCC 2 cut(s) 94, 360
BspMAI CTGCAG 1 cut(s) 377
BspOI GCTAGC 1 cut(s) 65
BspPI GGATC 3 cut(s) 163, 353, 366
BspT107I GGYRCC 1 cut(s) 92
BspTI CTTAAG 1 cut(s) 287
BsrI ACTGG 1 cut(s) 368
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 3 cut(s) 168, 190, 358
BssNAI GTATAC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 419
Bst1107I GTATAC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 132
BstAFI CTTAAG 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 63, 151
BstENI CCTNNNNNAGG 1 cut(s) 43
BstF5I GGATG 1 cut(s) 107
BstKTI GATC 3 cut(s) 171, 193, 361
BstMBI GATC 3 cut(s) 168, 190, 358
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 373
BstX2I RGATCY 2 cut(s) 168, 358
BstYI RGATCY 2 cut(s) 168, 358
BstZ17I GTATAC 1 cut(s) 35
BsuRI GGCC 2 cut(s) 350, 418
BtsCI GGATG 1 cut(s) 107
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 1 cut(s) 305
Cac8I GCNNGC 2 cut(s) 63, 151
Cfr13I GGNCC 3 cut(s) 20, 269, 416
CviAII CATG 3 cut(s) 185, 352, 413
CviJI RGCY 7 cut(s) 65, 126, 203, 238, 350, 386, 418
CviKI_1 RGCY 7 cut(s) 65, 126, 203, 238, 350, 386, 418
DpnI GATC 3 cut(s) 170, 192, 360
DpnII GATC 3 cut(s) 168, 190, 358
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 419
Eco32I GATATC 1 cut(s) 208
Eco47I GGWCC 2 cut(s) 20, 269
Eco57I CTGAAG 2 cut(s) 147, 317
EcoNI CCTNNNNNAGG 1 cut(s) 43
EcoO109I RGGNCCY 2 cut(s) 20, 269
EcoRII CCWGG 1 cut(s) 313
EcoRV GATATC 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 419
EcoT22I ATGCAT 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 3 cut(s) 188, 355, 416
FaiI YATR 8 cut(s) 35, 156, 158, 186, 230, 234, 353, 414
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FatI CATG 3 cut(s) 184, 351, 412
FblI GTMKAC 1 cut(s) 34
FokI GGATG 1 cut(s) 114
FspBI CTAG 2 cut(s) 62, 420
HaeIII GGCC 2 cut(s) 350, 418
HapII CCGG 1 cut(s) 260
Hin1II CATG 3 cut(s) 188, 355, 416
HpaII CCGG 1 cut(s) 260
HphI GGTGA 2 cut(s) 155, 243
Hpy166II GTNNAC 2 cut(s) 35, 251
Hpy188I TCNGA 3 cut(s) 45, 166, 336
Hpy188III TCNNGA 3 cut(s) 71, 172, 194
Hpy8I GTNNAC 2 cut(s) 35, 251
HpyAV CCTTC 2 cut(s) 171, 260
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4V TGCA 4 cut(s) 107, 328, 375, 412
Hsp92II CATG 3 cut(s) 188, 355, 416
Kzo9I GATC 3 cut(s) 168, 190, 358
LpnPI CCDG 6 cut(s) 84, 185, 273, 300, 327, 381
LweI GCATC 1 cut(s) 136
MaeI CTAG 2 cut(s) 62, 420
MaeIII GTNAC 2 cut(s) 255, 317
MalI GATC 3 cut(s) 170, 192, 360
MboI GATC 3 cut(s) 168, 190, 358
MboII GAAGA 3 cut(s) 149, 202, 275
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 2 cut(s) 168, 358
MluCI AATT 4 cut(s) 13, 132, 299, 439
MmeI TCCRAC 1 cut(s) 336
MnlI CCTC 4 cut(s) 39, 133, 239, 282
Mph1103I ATGCAT 1 cut(s) 414
MseI TTAA 2 cut(s) 281, 288
MspCI CTTAAG 1 cut(s) 287
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 315
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 315
NdeII GATC 3 cut(s) 168, 190, 358
NheI GCTAGC 1 cut(s) 61
NlaIII CATG 3 cut(s) 188, 355, 416
NlaIV GGNNCC 2 cut(s) 94, 360
NmuCI GTSAC 1 cut(s) 317
NsiI ATGCAT 1 cut(s) 414
PflMI CCANNNNNTGG 1 cut(s) 413
PpuMI RGGWCCY 2 cut(s) 20, 269
Psp5II RGGWCCY 2 cut(s) 20, 269
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 2 cut(s) 94, 360
PspPI GGNCC 3 cut(s) 20, 269, 416
PspPPI RGGWCCY 2 cut(s) 20, 269
PstI CTGCAG 1 cut(s) 377
PsuI RGATCY 2 cut(s) 168, 358
SaqAI TTAA 2 cut(s) 281, 288
Sau3AI GATC 3 cut(s) 168, 190, 358
Sau96I GGNCC 3 cut(s) 20, 269, 416
ScrFI CCNGG 1 cut(s) 315
SetI ASST 7 cut(s) 25, 67, 240, 250, 274, 281, 388
SfaNI GCATC 1 cut(s) 136
SfcI CTRYAG 1 cut(s) 373
SinI GGWCC 2 cut(s) 20, 269
SmlI CTYRAG 2 cut(s) 287, 344
SmoI CTYRAG 2 cut(s) 287, 344
Sse9I AATT 4 cut(s) 13, 132, 299, 439
SspMI CTAG 2 cut(s) 62, 420
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 1 cut(s) 255
TasI AATT 4 cut(s) 13, 132, 299, 439
Tru1I TTAA 2 cut(s) 281, 288
Tru9I TTAA 2 cut(s) 281, 288
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 1 cut(s) 317
Tsp45I GTSAC 1 cut(s) 317
TspRI CASTG 1 cut(s) 312
Van91I CCANNNNNTGG 1 cut(s) 413
Vha464I CTTAAG 1 cut(s) 287
VpaK11BI GGWCC 2 cut(s) 20, 269
XagI CCTNNNNNAGG 1 cut(s) 43
XapI RAATTY 1 cut(s) 13
XmaJI CCTAGG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 34
XspI CTAG 2 cut(s) 62, 420
Zsp2I ATGCAT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.