Rw0G021490
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00991
OG0006958
Physical Location & Seq
Reverse (-)
3137 .. 6043
2907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G021490.1

Sequence Viewer

Length: 429 bp
ATGGCAACGGTGGGGCTGAGCGAGGCGGCAAACCATTTCTTCTTCTGTGACTCCTCCTCATCGCACCCAACAAAGGGTGCCTTTGGATGTGCAACACTACTTGATAAAAGCCTACCAATTGAAGAGGGTGATGCTCGCTATATGCCTTCAGAGATCCTGAATGAGAAACATGACGATCTTGACAAGGCTGATATCTTCTCATTGGGAGTTGCTATGTATGAGCTTATTAGAGGTTCACCGTTACCGGAAGAAGGACCTCAAACCTTAAATCTTAAGAAAGGAAAATTGCCACTGCTTCCTGGTCACTCCTTGCAACTTCAGAACTTGCTCAAGGCCATGTTGGATCCAAATCCAGTCTGCAGACCGTCAGCTAAAGAGTTGGTTGAAAATCTAATGTTCCACAGGGTGCTAAAAAATGCATGGGACTAG

Protein Analysis

142

Amino Acids

15.64

Weight (kDa)

5.49

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 28 - 130 2.1e-10 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AciI CCGC 1 cut(s) 26
AclWI GGATC 3 cut(s) 148, 338, 351
AcuI CTGAAG 2 cut(s) 132, 302
AdeI CACNNNGTG 1 cut(s) 406
AfiI CCNNNNNNNGG 3 cut(s) 73, 74, 251
AflII CTTAAG 1 cut(s) 272
AgsI TTSAA 2 cut(s) 122, 386
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 2 cut(s) 223, 371
AluI AGCT 2 cut(s) 223, 371
AlwI GGATC 3 cut(s) 148, 338, 351
AoxI GGCC 1 cut(s) 333
AspS9I GGNCC 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 140, 228
AvaII GGWCC 1 cut(s) 254
BamHI GGATCC 1 cut(s) 343
BanI GGYRCC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 300
BfaI CTAG 1 cut(s) 427
BfmI CTRYAG 1 cut(s) 358
BfrI CTTAAG 1 cut(s) 272
BisI GCNGC 1 cut(s) 27
BlpI GCTNAGC 1 cut(s) 17
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 79, 345
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 121
Bpu1102I GCTNAGC 1 cut(s) 17
BpuEI CTTGAG 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 348
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 74, 251
Bse1I ACTGG 1 cut(s) 353
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 92
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 3 cut(s) 73, 74, 251
BseMII CTCAG 1 cut(s) 8
BseNI ACTGG 1 cut(s) 353
BseRI GAGGAG 2 cut(s) 43, 46
BshFI GGCC 1 cut(s) 335
BshNI GGYRCC 1 cut(s) 77
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 3 cut(s) 73, 74, 251
BsnI GGCC 1 cut(s) 335
Bsp143I GATC 3 cut(s) 153, 175, 343
Bsp1720I GCTNAGC 1 cut(s) 17
BspACI CCGC 1 cut(s) 26
BspANI GGCC 1 cut(s) 335
BspCNI CTCAG 1 cut(s) 9
BspLI GGNNCC 2 cut(s) 79, 345
BspMAI CTGCAG 1 cut(s) 362
BspPI GGATC 3 cut(s) 148, 338, 351
BspT107I GGYRCC 1 cut(s) 77
BspTI CTTAAG 1 cut(s) 272
BsrI ACTGG 1 cut(s) 353
BssMI GATC 3 cut(s) 153, 175, 343
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 3 cut(s) 10, 240, 366
Bst6I CTCTTC 1 cut(s) 117
BstAFI CTTAAG 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 1 cut(s) 17
BstF5I GGATG 1 cut(s) 92
BstKTI GATC 3 cut(s) 156, 178, 346
BstMBI GATC 3 cut(s) 153, 175, 343
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 358
BstX2I RGATCY 2 cut(s) 153, 343
BstYI RGATCY 2 cut(s) 153, 343
BsuRI GGCC 1 cut(s) 335
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 92
BtsI GCAGTG 1 cut(s) 290
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 254
CviAII CATG 3 cut(s) 170, 337, 420
CviJI RGCY 6 cut(s) 16, 111, 188, 223, 335, 371
CviKI_1 RGCY 6 cut(s) 16, 111, 188, 223, 335, 371
DdeI CTNAG 1 cut(s) 17
DpnI GATC 3 cut(s) 155, 177, 345
DpnII GATC 3 cut(s) 153, 175, 343
DraIII CACNNNGTG 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 254
Eco57I CTGAAG 2 cut(s) 132, 302
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 298
EcoRV GATATC 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 421
FaeI CATG 3 cut(s) 173, 340, 423
FaiI YATR 7 cut(s) 141, 143, 171, 215, 219, 338, 421
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FatI CATG 3 cut(s) 169, 336, 419
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 1 cut(s) 427
GluI GCNGC 1 cut(s) 27
HaeIII GGCC 1 cut(s) 335
HapII CCGG 1 cut(s) 245
Hin1II CATG 3 cut(s) 173, 340, 423
HinfI GANTC 1 cut(s) 50
HpaII CCGG 1 cut(s) 245
HphI GGTGA 2 cut(s) 140, 228
Hpy166II GTNNAC 1 cut(s) 236
Hpy188I TCNGA 2 cut(s) 151, 321
Hpy188III TCNNGA 2 cut(s) 157, 179
Hpy8I GTNNAC 1 cut(s) 236
HpyAV CCTTC 2 cut(s) 156, 245
HpyCH4III ACNGT 3 cut(s) 10, 240, 366
HpyCH4V TGCA 4 cut(s) 92, 313, 360, 419
HpyF3I CTNAG 1 cut(s) 17
Hsp92II CATG 3 cut(s) 173, 340, 423
Kzo9I GATC 3 cut(s) 153, 175, 343
LpnPI CCDG 6 cut(s) 170, 258, 285, 312, 366, 388
LweI GCATC 1 cut(s) 121
MaeI CTAG 1 cut(s) 427
MaeIII GTNAC 3 cut(s) 47, 240, 302
MalI GATC 3 cut(s) 155, 177, 345
MboI GATC 3 cut(s) 153, 175, 343
MboII GAAGA 5 cut(s) 31, 34, 134, 187, 260
MfeI CAATTG 1 cut(s) 117
MflI RGATCY 2 cut(s) 153, 343
MluCI AATT 2 cut(s) 117, 284
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 6 cut(s) 16, 64, 67, 118, 224, 267
Mph1103I ATGCAT 1 cut(s) 421
MseI TTAA 2 cut(s) 266, 273
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 300
MunI CAATTG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 300
NdeII GATC 3 cut(s) 153, 175, 343
NlaIII CATG 3 cut(s) 173, 340, 423
NlaIV GGNNCC 2 cut(s) 79, 345
NmuCI GTSAC 2 cut(s) 47, 302
NsiI ATGCAT 1 cut(s) 421
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PpuMI RGGWCCY 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 2 cut(s) 79, 345
PspPI GGNCC 1 cut(s) 254
PspPPI RGGWCCY 1 cut(s) 254
PstI CTGCAG 1 cut(s) 362
PsuI RGATCY 2 cut(s) 153, 343
SaqAI TTAA 2 cut(s) 266, 273
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 3 cut(s) 153, 175, 343
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 300
SetI ASST 5 cut(s) 225, 235, 259, 266, 373
SfaNI GCATC 1 cut(s) 121
SfcI CTRYAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 272, 329
SmoI CTYRAG 2 cut(s) 272, 329
Sse9I AATT 2 cut(s) 117, 284
SsiI CCGC 1 cut(s) 26
SspMI CTAG 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 3 cut(s) 10, 240, 366
TasI AATT 2 cut(s) 117, 284
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 2 cut(s) 266, 273
Tru9I TTAA 2 cut(s) 266, 273
TscAI CASTG 1 cut(s) 297
TseFI GTSAC 2 cut(s) 47, 302
Tsp45I GTSAC 2 cut(s) 47, 302
TspRI CASTG 1 cut(s) 297
Vha464I CTTAAG 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 254
XspI CTAG 1 cut(s) 427
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.