Rmu_sc0003892.1_g000020

CorA-like Mg2+ transporter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003892.1
OG0002432
Physical Location & Seq
Reverse (-)
99854 .. 105830
5977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1818 bp
atggcagctgtcaatttttgtaacggagctaacggctccaggtaccaatcttcggtcggggttagaatgtcaggtaccattctgatattttctgaacgtgaggtactaaagtttagaatggggcaaaggtcagaatcactctaccttctttccgcgatggaaaacagcaacaagagtaacacaggtgacaacatttctcatcccggtagcaataagctgaagaaaccagatggccataagaatggttatagtaaagatgtcatgcctggaagtgaactttggacagacgggcttatctgtgcttttgagttcattcgaggccgaaagaaaccaagtggtttgaaatctggctcaacaatcctaagcagacaacatacagatgttggtgaccatgagaagctgcgggttccttctaaaggatttcaagagtcttcttccccaatacatgataggaacaagtccctaagtggtgattacaaatacagcccagctcatgatggtgaaagagtgggtgatcattgggtgccaattgggtgggctagaatttcagaaatcgtccaaactgtgcaagctgatgccggttgggatcctcaacagtttgggattgatgatgaagatgacttcactgttgcagacttagcagctccatattgggagcgtccagtggggcctgtttggtggtgccatgtctctgcaggtcacccctcagttgatgattggctccgtaatgcccagtggctacatcctgccgtcagtttagctttaagagatgaaagtcgactgattagtgacaggatgaagcaccttttatatgaggtcccagttagagtttctggggggttgttatttgagctcttgggacagtcagttggtaatccatttgttgacgaggatgatatacccattgtacttcgttcctggcaagcacaaaacttccttgtaactgctttgcatgtgaaagggcctgtatcaagtataaatgtgttgggcatcacagaagttcaggaacttctttcgactggaggctataatgtaccaagaacagtgcatgaagtcatagcacatctaattagccgccttactcgatgggatgataggctattccgcaagtccatatttggtgccgcagatgagattgaattgaagtttatgaacaggagaaaccatgaagatatgaatctctttagcataattctcaaccaagaaatcagaaggttatcgagacaggtcatcagagtaaagtggtcacttcatgcaagagaggagattttgtttgagcttcttgaacatttgagaggaaatactacaagaagcttgttggagaaaataagaaagagcacaagagaaatgattgaggagcaagaagcagttcgtggccgcttatttacaattcaggatgtgatgcagagcactgttcgtgcatggttgcaggacagaagcctaagagtgacacataatcttgctgtttttggcggctgtggccttgtactttccatcatcactgggctttttggaatcaatgttgatggaatgcctggagcaaacaatgcaccatatgcattcggcgtattttcagccgttctcttctttattggaatcgtgctaattctacttgggttgctttaccttgggttgaaacagcctattgctgaagagcaagttgaagttagaaagatggagcttcaggagttggttcagatgtttcagcacgaggcagagactcatgccctggttcgtaaaaatgtggctcggaataatttacccccaacaatgggagatgcatttgcacgtaatgcaaattatatcctcattgagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CorA

Protein Analysis

605

Amino Acids

68.15

Weight (kDa)

6.59

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CorA PF01544 401 - 537 9.3e-12 CorA-like Mg2+ transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 686
Acc65I GGTACC 2 cut(s) 42, 74
AccB1I GGYRCC 5 cut(s) 42, 74, 523, 681, 1121
AccI GTMKAC 1 cut(s) 778
AccII CGCG 1 cut(s) 155
AciI CCGC 7 cut(s) 153, 403, 1075, 1105, 1125, 1378, 1473
AclWI GGATC 2 cut(s) 581, 594
AcoI YGGCCR 2 cut(s) 232, 1375
AcsI RAATTY 1 cut(s) 543
AcuI CTGAAG 3 cut(s) 239, 1667, 1671
AfaI GTAC 6 cut(s) 44, 76, 105, 909, 1035, 1488
AfiI CCNNNNNNNGG 4 cut(s) 52, 416, 1771, 1772
AgsI TTSAA 7 cut(s) 343, 425, 1139, 1144, 1286, 1636, 1664
AjnI CCWGG 5 cut(s) 38, 265, 917, 1534, 1728
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
Alw21I GWGCWC 3 cut(s) 855, 1340, 1412
Alw26I GTCTC 3 cut(s) 694, 1216, 1712
AlwI GGATC 2 cut(s) 581, 594
AoxI GGCC 6 cut(s) 232, 319, 668, 962, 1375, 1480
ApeKI GCWGC 3 cut(s) 5, 400, 641
ApoI RAATTY 1 cut(s) 543
Asp700I GAANNNNTTC 1 cut(s) 1368
Asp718I GGTACC 2 cut(s) 42, 74
AspS9I GGNCC 3 cut(s) 668, 817, 962
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 6 cut(s) 197, 398, 482, 512, 524, 692
AvaII GGWCC 1 cut(s) 817
BaeI ACNNNNGTAYC 2 cut(s) 95, 128
BalI TGGCCA 1 cut(s) 234
BamHI GGATCC 1 cut(s) 586
BanI GGYRCC 5 cut(s) 42, 74, 523, 681, 1121
BanII GRGCYC 1 cut(s) 855
BauI CACGAG 1 cut(s) 1709
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 3 cut(s) 855, 1340, 1412
BbvI GCAGC 3 cut(s) 17, 387, 653
BccI CCATC 7 cut(s) 151, 224, 491, 1080, 1502, 1520, 1669
BceAI ACGGC 3 cut(s) 49, 734, 1562
BciT130I CCWGG 5 cut(s) 40, 267, 919, 1536, 1730
BclI TGATCA 1 cut(s) 514
BcnI CCSGG 1 cut(s) 204
BcoDI GTCTC 3 cut(s) 694, 1216, 1712
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 1 cut(s) 693
BfuAI ACCTGC 1 cut(s) 686
BisI GCNGC 7 cut(s) 6, 401, 642, 1075, 1125, 1378, 1474
BlsI GCNGC 7 cut(s) 7, 402, 643, 1076, 1126, 1379, 1475
Bme1390I CCNGG 6 cut(s) 40, 204, 267, 919, 1536, 1730
Bme18I GGWCC 1 cut(s) 817
BmgT120I GGNCC 3 cut(s) 668, 817, 962
BmrFI CCNGG 6 cut(s) 40, 204, 267, 919, 1536, 1730
BmrI ACTGGG 3 cut(s) 727, 815, 1512
BmsI GCATC 4 cut(s) 565, 999, 1392, 1768
BmuI ACTGGG 3 cut(s) 727, 815, 1512
BpiI GAAGAC 1 cut(s) 423
BpmI CTGGAG 3 cut(s) 22, 1041, 1557
Bpu10I CCTNAGC 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 204
BsaAI YACGTR 1 cut(s) 1790
BsaBI GATNNNNATC 1 cut(s) 1176
BsaJI CCNNGG 2 cut(s) 1627, 1728
BsaXI ACNNNNNCTCC 2 cut(s) 1256, 1286
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 416, 1771, 1772
Bse118I RCCGGY 1 cut(s) 578
Bse1I ACTGG 5 cut(s) 662, 733, 821, 1024, 1507
Bse8I GATNNNNATC 1 cut(s) 1176
BseBI CCWGG 5 cut(s) 40, 267, 919, 1536, 1730
BseDI CCNNGG 2 cut(s) 1627, 1728
BseGI GGATG 6 cut(s) 199, 742, 801, 898, 1096, 1402
BseJI GATNNNNATC 1 cut(s) 1176
BseLI CCNNNNNNNGG 4 cut(s) 52, 416, 1771, 1772
BseMII CTCAG 1 cut(s) 720
BseNI ACTGG 5 cut(s) 662, 733, 821, 1024, 1507
BseRI GAGGAG 2 cut(s) 1277, 1370
BseXI GCAGC 3 cut(s) 17, 387, 653
BseYI CCCAGC 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 6 cut(s) 234, 321, 670, 964, 1377, 1482
BshNI GGYRCC 5 cut(s) 42, 74, 523, 681, 1121
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 3 cut(s) 855, 1340, 1412
BsiSI CCGG 2 cut(s) 204, 579
BslFI GGGAC 3 cut(s) 445, 803, 873
BslI CCNNNNNNNGG 4 cut(s) 52, 416, 1771, 1772
BsmAI GTCTC 3 cut(s) 694, 1216, 1712
BsmFI GGGAC 3 cut(s) 445, 803, 873
BsmI GAATGC 2 cut(s) 1536, 1559
BsnI GGCC 6 cut(s) 234, 321, 670, 964, 1377, 1482
Bsp1286I GDGCHC 3 cut(s) 855, 1340, 1412
Bsp143I GATC 2 cut(s) 514, 586
BspACI CCGC 7 cut(s) 153, 403, 1075, 1105, 1125, 1378, 1473
BspANI GGCC 6 cut(s) 234, 321, 670, 964, 1377, 1482
BspCNI CTCAG 1 cut(s) 719
BspFNI CGCG 1 cut(s) 155
BspHI TCATGA 1 cut(s) 493
BspMAI CTGCAG 1 cut(s) 697
BspMI ACCTGC 1 cut(s) 686
BspPI GGATC 2 cut(s) 581, 594
BspQI GCTCTTC 1 cut(s) 1647
BspT107I GGYRCC 5 cut(s) 42, 74, 523, 681, 1121
BsrFI RCCGGY 1 cut(s) 578
BsrI ACTGG 5 cut(s) 662, 733, 821, 1024, 1507
BssAI RCCGGY 1 cut(s) 578
BssECI CCNNGG 2 cut(s) 1627, 1728
BssMI GATC 2 cut(s) 514, 586
BssSI CACGAG 1 cut(s) 1709
BssT1I CCWWGG 1 cut(s) 1627
Bst2BI CACGAG 1 cut(s) 1709
Bst2UI CCWGG 5 cut(s) 40, 267, 919, 1536, 1730
Bst4CI ACNGT 6 cut(s) 565, 597, 628, 864, 1045, 1414
Bst6I CTCTTC 2 cut(s) 1589, 1647
BstAPI GCANNNNNTGC 3 cut(s) 1547, 1556, 1793
BstBAI YACGTR 1 cut(s) 1790
BstC8I GCNNGC 2 cut(s) 570, 924
BstDEI CTNAG 5 cut(s) 362, 464, 637, 706, 1442
BstEII GGTNACC 2 cut(s) 386, 698
BstENI CCTNNNNNAGG 1 cut(s) 414
BstF5I GGATG 6 cut(s) 199, 742, 801, 898, 1096, 1402
BstFNI CGCG 1 cut(s) 155
BstKTI GATC 2 cut(s) 517, 589
BstMAI GTCTC 3 cut(s) 694, 1216, 1712
BstMBI GATC 2 cut(s) 514, 586
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 5 cut(s) 638, 1479, 1547, 1556, 1793
BstNI CCWGG 5 cut(s) 40, 267, 919, 1536, 1730
BstNSI RCATGY 1 cut(s) 956
BstPI GGTNACC 2 cut(s) 386, 698
BstSCI CCNGG 6 cut(s) 38, 202, 265, 917, 1534, 1728
BstSFI CTRYAG 1 cut(s) 693
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 3 cut(s) 17, 387, 653
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 586
BstXI CCANNNNNNTGG 2 cut(s) 242, 534
BstYI RGATCY 1 cut(s) 586
BsuRI GGCC 6 cut(s) 234, 321, 670, 964, 1377, 1482
BtgZI GCGATG 1 cut(s) 170
BtsCI GGATG 6 cut(s) 199, 742, 801, 898, 1096, 1402
BtsIMutI CAGTG 6 cut(s) 624, 669, 740, 1050, 1410, 1500
BveI ACCTGC 1 cut(s) 686
Cac8I GCNNGC 2 cut(s) 570, 924
CciI TCATGA 1 cut(s) 493
Cfr10I RCCGGY 1 cut(s) 578
Cfr13I GGNCC 3 cut(s) 668, 817, 962
CseI GACGC 1 cut(s) 647
Csp6I GTAC 6 cut(s) 43, 75, 104, 908, 1034, 1487
CviQI GTAC 6 cut(s) 43, 75, 104, 908, 1034, 1487
DdeI CTNAG 5 cut(s) 362, 464, 637, 706, 1442
DpnI GATC 2 cut(s) 516, 588
DpnII GATC 2 cut(s) 514, 586
EaeI YGGCCR 2 cut(s) 232, 1375
Eam1104I CTCTTC 2 cut(s) 1589, 1647
EarI CTCTTC 2 cut(s) 1589, 1647
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 1 cut(s) 1627
Eco24I GRGCYC 1 cut(s) 855
Eco47I GGWCC 1 cut(s) 817
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 3 cut(s) 239, 1667, 1671
Eco91I GGTNACC 2 cut(s) 386, 698
EcoICRI GAGCTC 1 cut(s) 853
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoO109I RGGNCCY 3 cut(s) 668, 817, 962
EcoO65I GGTNACC 2 cut(s) 386, 698
EcoRII CCWGG 5 cut(s) 38, 265, 917, 1534, 1728
EcoT14I CCWWGG 1 cut(s) 1627
EcoT22I ATGCAT 2 cut(s) 1561, 1783
EcoT38I GRGCYC 1 cut(s) 855
ErhI CCWWGG 1 cut(s) 1627
FaqI GGGAC 3 cut(s) 445, 803, 873
FauI CCCGC 1 cut(s) 396
FauNDI CATATG 1 cut(s) 1555
FbaI TGATCA 1 cut(s) 514
FblI GTMKAC 1 cut(s) 778
Fnu4HI GCNGC 7 cut(s) 6, 401, 642, 1075, 1125, 1378, 1474
FokI GGATG 6 cut(s) 186, 729, 808, 905, 1103, 1409
FriOI GRGCYC 1 cut(s) 855
Fsp4HI GCNGC 7 cut(s) 6, 401, 642, 1075, 1125, 1378, 1474
FspBI CTAG 1 cut(s) 540
GluI GCNGC 7 cut(s) 6, 401, 642, 1075, 1125, 1378, 1474
GsaI CCCAGC 1 cut(s) 491
GsuI CTGGAG 3 cut(s) 22, 1041, 1557
HaeIII GGCC 6 cut(s) 234, 321, 670, 964, 1377, 1482
HapII CCGG 2 cut(s) 204, 579
HgaI GACGC 1 cut(s) 647
HincII GTYRAC 2 cut(s) 779, 886
HindII GTYRAC 2 cut(s) 779, 886
HindIII AAGCTT 1 cut(s) 1312
HinfI GANTC 6 cut(s) 134, 428, 1177, 1515, 1596, 1720
HpaII CCGG 2 cut(s) 204, 579
HphI GGTGA 6 cut(s) 197, 398, 482, 512, 524, 692
Hpy166II GTNNAC 3 cut(s) 275, 779, 886
Hpy188I TCNGA 8 cut(s) 84, 94, 133, 550, 1211, 1235, 1698, 1752
Hpy188III TCNNGA 7 cut(s) 425, 494, 1004, 1221, 1283, 1394, 1685
Hpy8I GTNNAC 3 cut(s) 275, 779, 886
HpyAV CCTTC 3 cut(s) 155, 420, 1206
HpyCH4III ACNGT 6 cut(s) 565, 597, 628, 864, 1045, 1414
HpyCH4IV ACGT 2 cut(s) 97, 1789
HpyF10VI GCNNNNNNNGC 5 cut(s) 638, 1479, 1547, 1556, 1793
HpyF3I CTNAG 5 cut(s) 362, 464, 637, 706, 1442
HpySE526I ACGT 2 cut(s) 97, 1789
KpnI GGTACC 2 cut(s) 46, 78
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 2 cut(s) 514, 586
LguI GCTCTTC 1 cut(s) 1647
LmnI GCTCC 8 cut(s) 26, 41, 649, 655, 726, 1357, 1538, 1678
Lsp1109I GCAGC 3 cut(s) 17, 387, 653
LweI GCATC 4 cut(s) 565, 999, 1392, 1768
MaeI CTAG 1 cut(s) 540
MaeII ACGT 2 cut(s) 97, 1789
MaeIII GTNAC 9 cut(s) 20, 176, 185, 386, 698, 788, 940, 1245, 1447
MalI GATC 2 cut(s) 516, 588
MboI GATC 2 cut(s) 514, 586
MboII GAAGA 8 cut(s) 42, 232, 423, 426, 626, 1181, 1576, 1664
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 3 cut(s) 855, 1340, 1412
MlsI TGGCCA 1 cut(s) 234
MluNI TGGCCA 1 cut(s) 234
MlyI GAGTC 2 cut(s) 437, 1714
MmeI TCCRAC 1 cut(s) 1299
Mox20I TGGCCA 1 cut(s) 234
Mph1103I ATGCAT 2 cut(s) 1561, 1783
MroXI GAANNNNTTC 1 cut(s) 1368
MscI TGGCCA 1 cut(s) 234
MseI TTAA 1 cut(s) 764
MslI CAYNNNNRTG 3 cut(s) 240, 378, 498
Msp20I TGGCCA 1 cut(s) 234
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 204, 579
MspR9I CCNGG 6 cut(s) 40, 204, 267, 919, 1536, 1730
MunI CAATTG 1 cut(s) 528
Mva1269I GAATGC 2 cut(s) 1536, 1559
MvaI CCWGG 5 cut(s) 40, 267, 919, 1536, 1730
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 5 cut(s) 638, 1479, 1547, 1556, 1793
NciI CCSGG 1 cut(s) 204
NdeI CATATG 1 cut(s) 1555
NdeII GATC 2 cut(s) 514, 586
NmuCI GTSAC 6 cut(s) 185, 386, 698, 788, 1245, 1447
NsiI ATGCAT 2 cut(s) 1561, 1783
NspI RCATGY 1 cut(s) 956
PagI TCATGA 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 1647
PctI GAATGC 2 cut(s) 1536, 1559
PdmI GAANNNNTTC 1 cut(s) 1368
PfeI GAWTC 4 cut(s) 134, 1177, 1515, 1596
PkrI GCNGC 7 cut(s) 7, 402, 643, 1076, 1126, 1379, 1475
PleI GAGTC 2 cut(s) 436, 1714
PpsI GAGTC 2 cut(s) 436, 1714
Ppu21I YACGTR 1 cut(s) 1790
PpuMI RGGWCCY 1 cut(s) 817
Psp124BI GAGCTC 1 cut(s) 855
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 5 cut(s) 38, 265, 917, 1534, 1728
PspEI GGTNACC 2 cut(s) 386, 698
PspFI CCCAGC 1 cut(s) 487
PspGI CCWGG 5 cut(s) 38, 265, 917, 1534, 1728
PspPI GGNCC 3 cut(s) 668, 817, 962
PspPPI RGGWCCY 1 cut(s) 817
PsrI GAACNNNNNNTAC 2 cut(s) 87, 119
PstI CTGCAG 1 cut(s) 697
PsuI RGATCY 1 cut(s) 586
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 6 cut(s) 44, 76, 105, 909, 1035, 1488
RsaNI GTAC 6 cut(s) 43, 75, 104, 908, 1034, 1487
RseI CAYNNNNRTG 3 cut(s) 240, 378, 498
SacI GAGCTC 1 cut(s) 855
SalI GTCGAC 1 cut(s) 777
SapI GCTCTTC 1 cut(s) 1647
SaqAI TTAA 1 cut(s) 764
SatI GCNGC 7 cut(s) 6, 401, 642, 1075, 1125, 1378, 1474
Sau3AI GATC 2 cut(s) 514, 586
Sau96I GGNCC 3 cut(s) 668, 817, 962
SchI GAGTC 2 cut(s) 437, 1714
ScrFI CCNGG 6 cut(s) 40, 204, 267, 919, 1536, 1730
SduI GDGCHC 3 cut(s) 855, 1340, 1412
SfaNI GCATC 4 cut(s) 565, 999, 1392, 1768
SfcI CTRYAG 1 cut(s) 693
SinI GGWCC 1 cut(s) 817
SmiMI CAYNNNNRTG 3 cut(s) 240, 378, 498
SsiI CCGC 7 cut(s) 153, 403, 1075, 1105, 1125, 1378, 1473
SspMI CTAG 1 cut(s) 540
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 6 cut(s) 38, 202, 265, 917, 1534, 1728
StyI CCWWGG 1 cut(s) 1627
TaaI ACNGT 6 cut(s) 565, 597, 628, 864, 1045, 1414
TaiI ACGT 2 cut(s) 100, 1792
TaqI TCGA 5 cut(s) 316, 778, 1016, 1084, 1220
TaqII GACCGA 1 cut(s) 43
TatI WGTACW 2 cut(s) 907, 1486
TauI GCSGC 4 cut(s) 1077, 1127, 1380, 1476
TfiI GAWTC 4 cut(s) 134, 1177, 1515, 1596
Tru1I TTAA 1 cut(s) 764
Tru9I TTAA 1 cut(s) 764
TscAI CASTG 6 cut(s) 631, 669, 740, 1050, 1417, 1507
TseFI GTSAC 6 cut(s) 185, 386, 698, 788, 1245, 1447
TseI GCWGC 3 cut(s) 5, 400, 641
Tsp45I GTSAC 6 cut(s) 185, 386, 698, 788, 1245, 1447
TspDTI ATGAA 9 cut(s) 301, 627, 786, 812, 1065, 1166, 1182, 1190, 1241
TspGWI ACGGA 2 cut(s) 39, 713
TspRI CASTG 6 cut(s) 631, 669, 740, 1050, 1417, 1507
VpaK11BI GGWCC 1 cut(s) 817
XagI CCTNNNNNAGG 1 cut(s) 414
XapI RAATTY 1 cut(s) 543
XceI RCATGY 1 cut(s) 956
XmiI GTMKAC 1 cut(s) 778
XmnI GAANNNNTTC 1 cut(s) 1368
XspI CTAG 1 cut(s) 540
Zsp2I ATGCAT 2 cut(s) 1561, 1783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.