Rmu_sc0004134.1_g000007

PAS fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004134.1
OG0004648
Physical Location & Seq
Reverse (-)
22742 .. 30517
7776 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3081 bp
atggaagacgaacattccgaaacgccagcctcactcattcccccactcccaagagactcccgcggctccctcgaagtcttcaacccctcctcctcgacccgacccgccagccccccacccttccgaccttcccaacccgcctcctggaacacctggatcgacccacgtgaccccaagcccgacccgccacccaacgccgcctccaaggccggccgcgacggcgacgtcgacggcgacggcaccatcacctcatggatggccctcaaggacttccccccaactccaccgccgccgtccaagccgtccaaacagtcccagcaatcgccgccgctcatccagcagaccatctccgcctcggccaacgacaagtcatcgacggagcagagcgacgcggcgcagagggccgccgagtggggattggttctgaagacggacacggagacggggaggccgcagggagtgaccacccggaactcgggcggacccgaagacccgagccacaaaccgggtacctccagaagaaactccaacaactcggtccggagctccggggagttgtccgacgacggcggtggcggaggaggaggagggaaagggtttccgagagcttcggaggacttgaagaacgtgctgtcgacgtttcagcagacgttcgtggtgtcggatgctaccaaacccgattacccgatcatgtacgccagcgccgggtttttcaagatgaccgggtacacctccaaggaggtcatcggcaggaactgccggtttttgcagggagcggatacggacccggacgacgtggcgcagctgagggaggctttgcagaagaacacgagctattgcggtcggttgttgaattataagaaagatgggacccctttttggaatttacttactattgctcctatcaaggacgaaactgggaaggtcctcaaattcattggaatgcaagtggaggtgagcaagcacactgaggggtccaaggataagatgctgcgccccaatggattgcctgaatctctcattcgatatgatgctcgtcagaaagagatggctacacattcagtaactgagcttgtgcaggcggtgaggagaccgaggtcacttagtgaatcgacaaatcgtcccttcagaaagtccggaggtggcggtaggggagaggaggtaatagaagctcttgcgaggagaaactccgacagtttgcctaggagaaactcagagaatgtggcaccacctagacgtaactcacttggggatgctaaaatctcaatgcagtgtatcaatgaggttcctgaaaagaaacagaaaaaatctagacggcgctctttcatgggggttattcggaaaagtcaaactcaaagccaaaccgatgatgatacatttgacaactttggagattctgagaatgtccgtgatgacagtgacaatgatgagggacctatgagtcttgaagataaagcgaggaagaaggcaatgaggaagggtatggatcttgctacgacgctggaacgtatagagaaaaactttgtcattactgatccaaggctgcctgataatcccattatatttgcatccgacagcttcttagagctaacggagtacagccgcgaagaaatattgggaagaaattgcagattccttcaaggacctgagactgatccagtaactgtgaaaaaaataagagaggcaattgacaaccagactgacgtgactgtacaactcatcaattacacgaaaagcggcaaaaagttctggaatgtctttcatctgcagcctatgcgtgatcaaaagggagaagtacagtattttattggagtacaacttgatggcagtcagcacattgagcctctgcataactctattcctgaagttacagcaaaagagagtgaaaaactggtcaaagagactgcagttaatgttgatgaggcagcaagagaacttccagatgccaatatgaaaccagaagatttatggatgaatcattcgaaggtcgttcatccaaagcctcatagaagggacagccccccttggagagctattcaaaagattctagacagtggagaacagataggattgaaacattttaagcctataaggcccttgggatctggtgatactggcagtgtgcatttggtccaattgtgtggaactgatcagtactacgccatgaaggctatggataagggtatcatgctcaataggaacaaggtgcacagagcttgtgcggagagagaaatcctcgacatgttggatcacccttttcttcctgcattgtatgcttcgtttcagaccaaaacacatgtttgcctgatcactgattactaccctggtggagaactcttcctacttttggacagccaaccaacgaaggtcctgaaggaagattcagtgagattttatgttacagaggtagttgttgccttggagtatcttcactgtcaagggataatttacagggatttgaagcctgaaaatgttttacttcagagcagtgggcacgtaaccctgaccgattttgatttatcgtgtttgacatcttgcaaaccacagcttttaattcctaccataaacgaaaagaaaaggcatcacaaaggtcaacagaatccaatcttcatggctgaaccaatgagagcatcaaattcttttgttggcacggaagagtacatcgctccggaaatcataactggagctggccattctagtgcagtagactggtgggctcttggtattcttatatatgaaatgctctatggatatacaccatttaggggaaagacaaggcaaaagacgttcgccaatattcttcacaaggatctcaaatttccaggaagtataccggcaagtctcccagcaaaacagttaatgtatcggttgttgcatcgtgatcccaaaaacagattggggtctcaggaaggggcaagcgaaattaagcgtcaccctttctttcggggtgtcaactgggctctagttcgctgcatgaaccctcctcaactcgatgctcctctcttcgggacaactgaagctgaaaagggtgctaagcttgtggatcctgagatgcaagatctacaaacaaatattttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009657 GO:0009658 GO:0009785 GO:0009881 GO:0009882 GO:0009898 GO:0009902 GO:0009903 GO:0009904 GO:0009986 GO:0009987 GO:0010119 GO:0010155 GO:0010181 GO:0010359 GO:0010360 GO:0010361 GO:0010362 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019750 GO:0022898 GO:0023052 GO:0030522 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032553 GO:0032879 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0035556 GO:0036094 GO:0036211 GO:0038023 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0044070 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046777 GO:0048037 GO:0048511 GO:0048519 GO:0050662 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051649 GO:0051656 GO:0051667 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0104004 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903792 GO:1903959 GO:1903960 GO:1904062
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9 Pkinase

Protein Analysis

1026

Amino Acids

114.55

Weight (kDa)

7.87

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 224 - 320 3.4e-20 PAS domain
PAS PF00989 229 - 315 7.4e-08 PAS fold
PAS_3 PF08447 230 - 314 2.5e-07 PAS fold
PAS_9 PF13426 516 - 608 1e-19 PAS domain
PAS PF00989 519 - 608 1.6e-06 PAS fold
PAS_3 PF08447 520 - 605 6e-07 PAS fold
Pkinase PF00069 696 - 981 1.5e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 698 - 843 1.4e-16 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 886 - 966 1.4e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 858
AasI GACNNNNNNGTC 2 cut(s) 224, 1440
AatII GACGTC 1 cut(s) 228
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 3 cut(s) 239, 509, 1225
AccBSI CCGCTC 2 cut(s) 331, 776
AccI GTMKAC 4 cut(s) 228, 635, 2706, 2830
AccII CGCG 4 cut(s) 63, 216, 392, 1605
AccIII TCCGGA 3 cut(s) 540, 1136, 2668
AcoI YGGCCR 3 cut(s) 211, 357, 2689
AcsI RAATTY 4 cut(s) 883, 932, 2635, 2816
AcuI CTGAAG 6 cut(s) 446, 1111, 1884, 2393, 2465, 3036
AcvI CACGTG 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 225
AdeI CACNNNGTG 1 cut(s) 1106
AfaI GTAC 9 cut(s) 511, 695, 728, 1598, 1713, 1797, 1815, 2156, 2660
AfiI CCNNNNNNNGG 9 cut(s) 144, 411, 475, 505, 705, 879, 2347, 2765, 3005
AflIII ACRYGT 2 cut(s) 2241, 2296
AgsI TTSAA 9 cut(s) 82, 622, 715, 853, 1448, 1640, 2039, 2074, 2461
AhdI GACNNNNNGTC 1 cut(s) 1119
AjiI CACGTC 2 cut(s) 796, 1705
AjnI CCWGG 4 cut(s) 143, 152, 2323, 2821
Alw21I GWGCWC 2 cut(s) 548, 2211
Alw26I GTCTC 7 cut(s) 48, 434, 1084, 1643, 1895, 2846, 2907
Alw44I GTGCAC 1 cut(s) 2207
AlwNI CAGNNNCTG 3 cut(s) 756, 1067, 1664
Ama87I CYCGRG 2 cut(s) 475, 493
Aor13HI TCCGGA 3 cut(s) 540, 1136, 2668
AoxI GGCC 8 cut(s) 207, 211, 258, 357, 402, 449, 2093, 2689
ApaLI GTGCAC 1 cut(s) 2207
ApeKI GCWGC 6 cut(s) 802, 991, 1543, 1768, 1925, 2970
ApoI RAATTY 4 cut(s) 883, 932, 2635, 2816
Asp718I GGTACC 1 cut(s) 509
AspA2I CCTAGG 1 cut(s) 1202
AspLEI GCGC 5 cut(s) 397, 704, 802, 996, 1320
AsuC2I CCSGG 6 cut(s) 469, 507, 550, 706, 724, 788
AsuHPI GGTGA 6 cut(s) 238, 967, 1096, 2120, 2243, 2924
AsuII TTCGAA 1 cut(s) 1982
AvaI CYCGRG 2 cut(s) 475, 493
AvrII CCTAGG 1 cut(s) 1202
BaeGI GKGCMC 2 cut(s) 2211, 2496
BalI TGGCCA 1 cut(s) 2691
BamHI GGATCC 1 cut(s) 3043
BanI GGYRCC 3 cut(s) 239, 509, 1225
BanII GRGCYC 3 cut(s) 548, 2719, 2962
BauI CACGAG 1 cut(s) 829
BbrPI CACGTG 1 cut(s) 167
BbsI GAAGAC 4 cut(s) 12, 70, 434, 495
Bbv12I GWGCWC 2 cut(s) 548, 2211
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 6 cut(s) 814, 978, 1530, 1780, 1937, 2957
BccI CCATC 6 cut(s) 250, 251, 353, 860, 1042, 1817
BceAI ACGGC 7 cut(s) 235, 247, 253, 277, 286, 583, 1331
BciT130I CCWGG 4 cut(s) 145, 154, 2325, 2823
BciVI GTATCC 1 cut(s) 772
BclI TGATCA 3 cut(s) 1780, 2149, 2307
BcnI CCSGG 6 cut(s) 469, 507, 550, 706, 724, 788
BcoDI GTCTC 7 cut(s) 48, 434, 1084, 1643, 1895, 2846, 2907
BfaI CTAG 6 cut(s) 1203, 1233, 1311, 2048, 2697, 2963
BfmI CTRYAG 2 cut(s) 1766, 1905
BfoI RGCGCY 2 cut(s) 705, 1321
BfuI GTATCC 1 cut(s) 772
BglI GCCNNNNNGGC 4 cut(s) 206, 219, 2092, 2168
BglII AGATCT 1 cut(s) 3058
BlnI CCTAGG 1 cut(s) 1202
BlpI GCTNAGC 1 cut(s) 3033
BmcAI AGTACT 1 cut(s) 2156
BmeRI GACNNNNNGTC 1 cut(s) 1119
BmeT110I CYCGRG 2 cut(s) 475, 493
BmgBI CACGTC 2 cut(s) 796, 1705
BmrI ACTGGG 2 cut(s) 927, 2965
BmuI ACTGGG 2 cut(s) 927, 2965
BoxI GACNNNNGTC 1 cut(s) 1096
BpiI GAAGAC 4 cut(s) 12, 70, 434, 495
BplI GAGNNNNNCTC 2 cut(s) 2220, 2252
BpmI CTGGAG 2 cut(s) 499, 2703
Bpu10I CCTNAGC 1 cut(s) 806
Bpu1102I GCTNAGC 1 cut(s) 3033
Bpu14I TTCGAA 1 cut(s) 1982
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 6 cut(s) 469, 507, 550, 706, 724, 788
BsaAI YACGTR 2 cut(s) 167, 2497
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 2 cut(s) 1084, 2907
BsaWI WCCGGW 3 cut(s) 540, 1136, 2668
BsaXI ACNNNNNCTCC 8 cut(s) 74, 104, 185, 215, 883, 913, 1146, 1176
Bsc4I CCNNNNNNNGG 9 cut(s) 144, 411, 475, 505, 705, 879, 2347, 2765, 3005
Bse118I RCCGGY 3 cut(s) 209, 759, 2833
Bse1I ACTGG 7 cut(s) 922, 1658, 1896, 2119, 2686, 2714, 2960
Bse3DI GCAATG 1 cut(s) 1476
BseAI TCCGGA 3 cut(s) 540, 1136, 2668
BseBI CCWGG 4 cut(s) 145, 154, 2325, 2823
BseGI GGATG 7 cut(s) 261, 333, 670, 1258, 1568, 1977, 1993
BseLI CCNNNNNNNGG 9 cut(s) 144, 411, 475, 505, 705, 879, 2347, 2765, 3005
BseMI GCAATG 1 cut(s) 1476
BseMII CTCAG 8 cut(s) 797, 960, 1059, 1227, 1389, 1638, 2918, 3039
BseNI ACTGG 7 cut(s) 922, 1658, 1896, 2119, 2686, 2714, 2960
BseSI GKGCMC 2 cut(s) 2211, 2496
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 6 cut(s) 814, 978, 1530, 1780, 1937, 2957
BseYI CCCAGC 2 cut(s) 315, 2845
BsgI GTGCAG 2 cut(s) 1097, 2721
Bsh1236I CGCG 4 cut(s) 63, 216, 392, 1605
Bsh1285I CGRYCG 2 cut(s) 214, 844
BshFI GGCC 8 cut(s) 209, 213, 260, 359, 404, 451, 2095, 2691
BshNI GGYRCC 3 cut(s) 239, 509, 1225
BsiEI CGRYCG 2 cut(s) 214, 844
BsiHKAI GWGCWC 2 cut(s) 548, 2211
BsiHKCI CYCGRG 2 cut(s) 475, 493
BslFI GGGAC 6 cut(s) 298, 883, 1107, 1446, 2027, 3022
BslI CCNNNNNNNGG 9 cut(s) 144, 411, 475, 505, 705, 879, 2347, 2765, 3005
BsmAI GTCTC 7 cut(s) 48, 434, 1084, 1643, 1895, 2846, 2907
BsmBI CGTCTC 1 cut(s) 434
BsmFI GGGAC 6 cut(s) 298, 883, 1107, 1446, 2027, 3022
BsmI GAATGC 1 cut(s) 948
BsnI GGCC 8 cut(s) 209, 213, 260, 359, 404, 451, 2095, 2691
Bso31I GGTCTC 2 cut(s) 1084, 2907
BsoBI CYCGRG 2 cut(s) 475, 493
Bsp119I TTCGAA 1 cut(s) 1982
Bsp1286I GDGCHC 5 cut(s) 548, 2211, 2496, 2719, 2962
Bsp13I TCCGGA 3 cut(s) 540, 1136, 2668
Bsp1407I TGTACA 1 cut(s) 1711
Bsp1720I GCTNAGC 1 cut(s) 3033
BspANI GGCC 8 cut(s) 209, 213, 260, 359, 404, 451, 2095, 2691
BspCNI CTCAG 8 cut(s) 798, 961, 1060, 1226, 1390, 1639, 2917, 3040
BspEI TCCGGA 3 cut(s) 540, 1136, 2668
BspFNI CGCG 4 cut(s) 63, 216, 392, 1605
BspMAI CTGCAG 2 cut(s) 1770, 1909
BspT104I TTCGAA 1 cut(s) 1982
BspT107I GGYRCC 3 cut(s) 239, 509, 1225
BspTNI GGTCTC 2 cut(s) 1084, 2907
BsrBI CCGCTC 2 cut(s) 331, 776
BsrDI GCAATG 1 cut(s) 1476
BsrFI RCCGGY 3 cut(s) 209, 759, 2833
BsrGI TGTACA 1 cut(s) 1711
BsrI ACTGG 7 cut(s) 922, 1658, 1896, 2119, 2686, 2714, 2960
BssAI RCCGGY 3 cut(s) 209, 759, 2833
BssNAI GTATAC 1 cut(s) 2831
BssNI GRCGYC 1 cut(s) 225
BssSI CACGAG 1 cut(s) 829
BssT1I CCWWGG 8 cut(s) 204, 735, 978, 1202, 1538, 2024, 2097, 2418
Bst1107I GTATAC 1 cut(s) 2831
Bst2BI CACGAG 1 cut(s) 829
Bst2UI CCWGG 4 cut(s) 145, 154, 2325, 2823
Bst4CI ACNGT 9 cut(s) 312, 1196, 1418, 1666, 1711, 1800, 2054, 2435, 2856
Bst6I CTCTTC 3 cut(s) 2342, 2649, 3008
BstACI GRCGYC 1 cut(s) 225
BstAPI GCANNNNNTGC 3 cut(s) 756, 1774, 2273
BstAUI TGTACA 1 cut(s) 1711
BstBAI YACGTR 2 cut(s) 167, 2497
BstBI TTCGAA 1 cut(s) 1982
BstC8I GCNNGC 8 cut(s) 27, 109, 211, 700, 962, 1080, 2689, 2917
BstDSI CCRYGG 1 cut(s) 61
BstF5I GGATG 7 cut(s) 261, 333, 670, 1258, 1568, 1977, 1993
BstFNI CGCG 4 cut(s) 63, 216, 392, 1605
BstH2I RGCGCY 2 cut(s) 705, 1321
BstHHI GCGC 5 cut(s) 397, 704, 802, 996, 1320
BstMAI GTCTC 7 cut(s) 48, 434, 1084, 1643, 1895, 2846, 2907
BstMCI CGRYCG 2 cut(s) 214, 844
BstNI CCWGG 4 cut(s) 145, 154, 2325, 2823
BstNSI RCATGY 2 cut(s) 2245, 2300
BstPAI GACNNNNGTC 1 cut(s) 1096
BstSFI CTRYAG 2 cut(s) 1766, 1905
BstSLI GKGCMC 2 cut(s) 2211, 2496
BstUI CGCG 4 cut(s) 63, 216, 392, 1605
BstV1I GCAGC 6 cut(s) 814, 978, 1530, 1780, 1937, 2957
BstV2I GAAGAC 4 cut(s) 12, 70, 434, 495
BstX2I RGATCY 5 cut(s) 1486, 2102, 2809, 3043, 3058
BstXI CCANNNNNNTGG 1 cut(s) 2141
BstYI RGATCY 5 cut(s) 1486, 2102, 2809, 3043, 3058
BstZ17I GTATAC 1 cut(s) 2831
BstZI CGGCCG 1 cut(s) 211
BsuI GTATCC 1 cut(s) 772
BsuRI GGCC 8 cut(s) 209, 213, 260, 359, 404, 451, 2095, 2691
BtgI CCRYGG 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 2647
BtrI CACGTC 2 cut(s) 796, 1705
BtsCI GGATG 7 cut(s) 261, 333, 670, 1258, 1568, 1977, 1993
BtsI GCAGTG 3 cut(s) 1277, 2125, 2494
BtsIMutI CAGTG 9 cut(s) 966, 1277, 1423, 2059, 2125, 2310, 2391, 2431, 2494
Cac8I GCNNGC 8 cut(s) 27, 109, 211, 700, 962, 1080, 2689, 2917
CaiI CAGNNNCTG 3 cut(s) 756, 1067, 1664
CfoI GCGC 5 cut(s) 397, 704, 802, 996, 1320
Cfr10I RCCGGY 3 cut(s) 209, 759, 2833
Cfr42I CCGCGG 1 cut(s) 64
CpoI CGGWCCG 1 cut(s) 538
CseI GACGC 3 cut(s) 398, 1507, 2918
Csp6I GTAC 9 cut(s) 510, 694, 727, 1597, 1712, 1796, 1814, 2155, 2659
CspI CGGWCCG 1 cut(s) 538
CviAII CATG 9 cut(s) 252, 691, 1327, 2164, 2188, 2242, 2297, 2611, 2974
CviQI GTAC 9 cut(s) 510, 694, 727, 1597, 1712, 1796, 1814, 2155, 2659
DraIII CACNNNGTG 1 cut(s) 1106
DrdI GACNNNNNNGTC 2 cut(s) 224, 1440
DriI GACNNNNNGTC 1 cut(s) 1119
DseDI GACNNNNNNGTC 2 cut(s) 224, 1440
EaeI YGGCCR 3 cut(s) 211, 357, 2689
EagI CGGCCG 1 cut(s) 211
Eam1104I CTCTTC 3 cut(s) 2342, 2649, 3008
Eam1105I GACNNNNNGTC 1 cut(s) 1119
EarI CTCTTC 3 cut(s) 2342, 2649, 3008
EciI GGCGGA 3 cut(s) 340, 495, 591
Ecl136II GAGCTC 1 cut(s) 546
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 8 cut(s) 204, 735, 978, 1202, 1538, 2024, 2097, 2418
Eco24I GRGCYC 3 cut(s) 548, 2719, 2962
Eco31I GGTCTC 2 cut(s) 1084, 2907
Eco52I CGGCCG 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 546
Eco57I CTGAAG 6 cut(s) 446, 1111, 1884, 2393, 2465, 3036
Eco72I CACGTG 1 cut(s) 167
Eco88I CYCGRG 2 cut(s) 475, 493
EcoICRI GAGCTC 1 cut(s) 546
EcoO109I RGGNCCY 6 cut(s) 870, 925, 1433, 1643, 2094, 2368
EcoRII CCWGG 4 cut(s) 143, 152, 2323, 2821
EcoT14I CCWWGG 8 cut(s) 204, 735, 978, 1202, 1538, 2024, 2097, 2418
EcoT38I GRGCYC 3 cut(s) 548, 2719, 2962
ErhI CCWWGG 8 cut(s) 204, 735, 978, 1202, 1538, 2024, 2097, 2418
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 9 cut(s) 255, 694, 1330, 2167, 2191, 2245, 2300, 2614, 2977
FaqI GGGAC 6 cut(s) 298, 883, 1107, 1446, 2027, 3022
FatI CATG 9 cut(s) 251, 690, 1326, 2163, 2187, 2241, 2296, 2610, 2973
FauI CCCGC 4 cut(s) 68, 112, 145, 192
FbaI TGATCA 3 cut(s) 1780, 2149, 2307
FblI GTMKAC 4 cut(s) 228, 635, 2706, 2830
FokI GGATG 7 cut(s) 268, 320, 677, 1265, 1555, 1980, 1984
FriOI GRGCYC 3 cut(s) 548, 2719, 2962
FseI GGCCGGCC 1 cut(s) 213
FspBI CTAG 6 cut(s) 1203, 1233, 1311, 2048, 2697, 2963
GlaI GCGC 5 cut(s) 396, 703, 801, 995, 1319
GsaI CCCAGC 2 cut(s) 319, 2849
GsuI CTGGAG 2 cut(s) 499, 2703
HaeII RGCGCY 2 cut(s) 705, 1321
HaeIII GGCC 8 cut(s) 209, 213, 260, 359, 404, 451, 2095, 2691
HgaI GACGC 3 cut(s) 398, 1507, 2918
HhaI GCGC 5 cut(s) 397, 704, 802, 996, 1320
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 9 cut(s) 255, 694, 1330, 2167, 2191, 2245, 2300, 2614, 2977
Hin6I GCGC 5 cut(s) 395, 702, 800, 994, 1318
HinP1I GCGC 5 cut(s) 395, 702, 800, 994, 1318
HincII GTYRAC 4 cut(s) 229, 636, 2594, 2953
HindII GTYRAC 4 cut(s) 229, 636, 2594, 2953
HindIII AAGCTT 1 cut(s) 3035
HphI GGTGA 6 cut(s) 238, 967, 1096, 2120, 2243, 2924
Hpy166II GTNNAC 8 cut(s) 229, 636, 729, 2209, 2594, 2707, 2831, 2953
Hpy8I GTNNAC 8 cut(s) 229, 636, 729, 2209, 2594, 2707, 2831, 2953
HpyCH4III ACNGT 9 cut(s) 312, 1196, 1418, 1666, 1711, 1800, 2054, 2435, 2856
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 9 cut(s) 255, 694, 1330, 2167, 2191, 2245, 2300, 2614, 2977
HspAI GCGC 5 cut(s) 395, 702, 800, 994, 1318
KflI GGGWCCC 1 cut(s) 870
Kpn2I TCCGGA 3 cut(s) 540, 1136, 2668
KpnI GGTACC 1 cut(s) 513
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Ksp22I TGATCA 3 cut(s) 1780, 2149, 2307
KspI CCGCGG 1 cut(s) 64
LmnI GCTCC 9 cut(s) 71, 379, 543, 551, 773, 904, 2671, 2684, 3001
Lsp1109I GCAGC 6 cut(s) 814, 978, 1530, 1780, 1937, 2957
MaeI CTAG 6 cut(s) 1203, 1233, 1311, 2048, 2697, 2963
MbiI CCGCTC 2 cut(s) 331, 776
MfeI CAATTG 2 cut(s) 1686, 2135
MflI RGATCY 5 cut(s) 1486, 2102, 2809, 3043, 3058
MhlI GDGCHC 5 cut(s) 548, 2211, 2496, 2719, 2962
MlsI TGGCCA 1 cut(s) 2691
MluNI TGGCCA 1 cut(s) 2691
MlyI GAGTC 2 cut(s) 50, 1450
MmeI TCCRAC 7 cut(s) 148, 552, 585, 642, 1215, 1596, 2226
Mox20I TGGCCA 1 cut(s) 2691
MroI TCCGGA 3 cut(s) 540, 1136, 2668
MroNI GCCGGC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 2691
MseI TTAA 5 cut(s) 1911, 2082, 2552, 2858, 2925
MslI CAYNNNNRTG 2 cut(s) 941, 2697
Msp20I TGGCCA 1 cut(s) 2691
MspA1I CMGCKG 2 cut(s) 63, 805
MunI CAATTG 2 cut(s) 1686, 2135
Mva1269I GAATGC 1 cut(s) 948
MvaI CCWGG 4 cut(s) 145, 154, 2325, 2823
MvnI CGCG 4 cut(s) 63, 216, 392, 1605
NaeI GCCGGC 1 cut(s) 211
NciI CCSGG 6 cut(s) 469, 507, 550, 706, 724, 788
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 9 cut(s) 255, 694, 1330, 2167, 2191, 2245, 2300, 2614, 2977
NmeAIII GCCGAG 2 cut(s) 335, 433
NmuCI GTSAC 6 cut(s) 167, 460, 1098, 1418, 1705, 2930
NspI RCATGY 2 cut(s) 2245, 2300
NspV TTCGAA 1 cut(s) 1982
PciI ACATGT 2 cut(s) 2241, 2296
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 225, 231
PctI GAATGC 1 cut(s) 948
PdiI GCCGGC 1 cut(s) 211
PfeI GAWTC 8 cut(s) 1013, 1109, 1394, 1632, 1975, 2044, 2381, 2599
PfoI TCCNGGA 2 cut(s) 143, 2821
PleI GAGTC 2 cut(s) 50, 1449
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
PpsI GAGTC 2 cut(s) 50, 1449
Ppu21I YACGTR 2 cut(s) 167, 2497
PpuMI RGGWCCY 5 cut(s) 870, 925, 1433, 1643, 2368
PscI ACATGT 2 cut(s) 2241, 2296
PshAI GACNNNNGTC 1 cut(s) 1096
PsiI TTATAA 1 cut(s) 858
Psp124BI GAGCTC 1 cut(s) 548
Psp5II RGGWCCY 5 cut(s) 870, 925, 1433, 1643, 2368
Psp6I CCWGG 4 cut(s) 143, 152, 2323, 2821
PspCI CACGTG 1 cut(s) 167
PspFI CCCAGC 2 cut(s) 315, 2845
PspGI CCWGG 4 cut(s) 143, 152, 2323, 2821
PspPPI RGGWCCY 5 cut(s) 870, 925, 1433, 1643, 2368
PsrI GAACNNNNNNTAC 2 cut(s) 2325, 2357
PstI CTGCAG 2 cut(s) 1770, 1909
PstNI CAGNNNCTG 3 cut(s) 756, 1067, 1664
PsuI RGATCY 5 cut(s) 1486, 2102, 2809, 3043, 3058
PvuII CAGCTG 1 cut(s) 805
RigI GGCCGGCC 1 cut(s) 213
RsaI GTAC 9 cut(s) 511, 695, 728, 1598, 1713, 1797, 1815, 2156, 2660
RsaNI GTAC 9 cut(s) 510, 694, 727, 1597, 1712, 1796, 1814, 2155, 2659
RseI CAYNNNNRTG 2 cut(s) 941, 2697
Rsr2I CGGWCCG 1 cut(s) 538
RsrII CGGWCCG 1 cut(s) 538
SacI GAGCTC 1 cut(s) 548
SacII CCGCGG 1 cut(s) 64
SalI GTCGAC 2 cut(s) 227, 634
SaqAI TTAA 5 cut(s) 1911, 2082, 2552, 2858, 2925
ScaI AGTACT 1 cut(s) 2156
SchI GAGTC 2 cut(s) 50, 1450
SduI GDGCHC 5 cut(s) 548, 2211, 2496, 2719, 2962
SfcI CTRYAG 2 cut(s) 1766, 1905
Sfr303I CCGCGG 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 1982
SgrBI CCGCGG 1 cut(s) 64
SgrDI CGTCGACG 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 941, 2697
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SspI AATATT 3 cut(s) 1614, 2797, 3073
SspMI CTAG 6 cut(s) 1203, 1233, 1311, 2048, 2697, 2963
SstI GAGCTC 1 cut(s) 548
StyI CCWWGG 8 cut(s) 204, 735, 978, 1202, 1538, 2024, 2097, 2418
TaaI ACNGT 9 cut(s) 312, 1196, 1418, 1666, 1711, 1800, 2054, 2435, 2856
TaqII GACCGA 3 cut(s) 526, 1108, 2522
TatI WGTACW 6 cut(s) 1596, 1711, 1795, 1813, 2154, 2658
TfiI GAWTC 8 cut(s) 1013, 1109, 1394, 1632, 1975, 2044, 2381, 2599
Tru1I TTAA 5 cut(s) 1911, 2082, 2552, 2858, 2925
Tru9I TTAA 5 cut(s) 1911, 2082, 2552, 2858, 2925
TscAI CASTG 9 cut(s) 973, 1277, 1423, 2059, 2125, 2317, 2391, 2438, 2494
TseFI GTSAC 6 cut(s) 167, 460, 1098, 1418, 1705, 2930
TseI GCWGC 6 cut(s) 802, 991, 1543, 1768, 1925, 2970
Tsp45I GTSAC 6 cut(s) 167, 460, 1098, 1418, 1705, 2930
TspGWI ACGGA 7 cut(s) 392, 446, 452, 797, 1397, 1607, 2666
TspRI CASTG 9 cut(s) 973, 1277, 1423, 2059, 2125, 2317, 2391, 2438, 2494
VneI GTGCAC 1 cut(s) 2207
XapI RAATTY 4 cut(s) 883, 932, 2635, 2816
XbaI TCTAGA 2 cut(s) 1310, 2047
XceI RCATGY 2 cut(s) 2245, 2300
XcmI CCANNNNNNNNNTGG 3 cut(s) 1965, 2170, 2893
XmaJI CCTAGG 1 cut(s) 1202
XmiI GTMKAC 4 cut(s) 228, 635, 2706, 2830
XspI CTAG 6 cut(s) 1203, 1233, 1311, 2048, 2697, 2963
ZraI GACGTC 1 cut(s) 226
ZrmI AGTACT 1 cut(s) 2156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.