Rmu_sc0004174.1_g000007

Non-structural maintenance of chromosomes element 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004174.1
OG0003611
Physical Location & Seq
Reverse (-)
24984 .. 31383
6400 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
atggatccgatttatagggctgctgcctccttaaggcgtgatgtggctggcagaaagttgatggtagcggtccggtcgaagtggtgcggtggttgcgggtttcaaagtatgacggaactgaacgcgaagcaccacccgactctgactcaagctctgctctctcgcggcccacttaaaagaggaccagttccaccgcacgttctcggaactcaccggagaaaactcaggtctccgtgttcgaaggatgattatcttctgaggataaacaagggtgggttcacttcgagttgcggggatgcaggaatcaatatgaagttttatctttgtacgtgtggggtgtgttatcatatgcaattgtgcattgtgtgcacgctggagcataatcctcgattcctcgtctacagatgtatagaagctatagtgcaagaagctgctcaaggaaccatatctagcactgatgctcttaatctacgattagagaatcaggtttgcattggtacatcatcacaatcacatggaggtccagttcctatccctactgcattgaagaacttttcaatgtcccagaaggaaaaaactcttgacgaatgtgtacgggataaatggctttccttcactgctgtggatcatgttggacttggtgtcagatctttccttgatctgctaatttggttctgcaataatgatgttccttcatgcgaagtgtgtaacgaagctggagtgaaggttgtctcttcctttggtgaaagagtttgtccaagttgtggtactcaatggcaatatacaggaccaaaagcagaagcagtagaagatgatgagccaaattgcgccactcagagccaaccacctgtggaatccaagagaaagaagctgagaacaagccgaattggtgatggatatataattgaatctagttcctctcaggcttccttacctggtcgtaatctgagaagatccactcgaaattccacccgcctctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMC_Nse1 zf-RING-like

Protein Analysis

326

Amino Acids

35.93

Weight (kDa)

9.05

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_Nse1 PF07574 134 - 221 2.2e-08 Nse1 non-SMC component of SMC5-6 complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 764
AccI GTMKAC 1 cut(s) 399
AccII CGCG 2 cut(s) 125, 165
AciI CCGC 7 cut(s) 68, 87, 96, 165, 194, 291, 973
AclWI GGATC 3 cut(s) 12, 633, 948
AcsI RAATTY 1 cut(s) 964
AfaI GTAC 4 cut(s) 328, 499, 594, 769
AfiI CCNNNNNNNGG 2 cut(s) 33, 764
AflII CTTAAG 1 cut(s) 31
AflIII ACRYGT 1 cut(s) 329
AgsI TTSAA 4 cut(s) 104, 547, 558, 908
AhdI GACNNNNNGTC 1 cut(s) 641
AjnI CCWGG 1 cut(s) 934
AleI CACNNNNGTG 1 cut(s) 620
AloI GAACNNNNNNTCC 2 cut(s) 510, 542
AluBI AGCT 5 cut(s) 152, 416, 431, 716, 871
AluI AGCT 5 cut(s) 152, 416, 431, 716, 871
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 2 cut(s) 234, 736
Alw44I GTGCAC 1 cut(s) 367
AlwI GGATC 3 cut(s) 12, 633, 948
AoxI GGCC 1 cut(s) 166
ApaLI GTGCAC 1 cut(s) 367
ApeKI GCWGC 3 cut(s) 20, 23, 431
ApoI RAATTY 1 cut(s) 964
AspLEI GCGC 1 cut(s) 830
AspS9I GGNCC 5 cut(s) 70, 167, 182, 521, 788
AsuHPI GGTGA 3 cut(s) 203, 755, 902
AsuII TTCGAA 1 cut(s) 239
AvaII GGWCC 4 cut(s) 70, 182, 521, 788
BaeGI GKGCMC 1 cut(s) 371
BamHI GGATCC 1 cut(s) 4
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 3 cut(s) 7, 10, 418
BccI CCATC 2 cut(s) 55, 887
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 1 cut(s) 936
BcoDI GTCTC 2 cut(s) 234, 736
BfaI CTAG 2 cut(s) 450, 912
BfmI CTRYAG 2 cut(s) 400, 417
BfrI CTTAAG 1 cut(s) 31
BglII AGATCT 1 cut(s) 647
BisI GCNGC 4 cut(s) 21, 24, 166, 432
BlsI GCNGC 4 cut(s) 22, 25, 167, 433
Bme1390I CCNGG 1 cut(s) 936
Bme18I GGWCC 4 cut(s) 70, 182, 521, 788
BmeRI GACNNNNNGTC 1 cut(s) 641
BmgT120I GGNCC 5 cut(s) 70, 167, 182, 521, 788
BmiI GGNNCC 2 cut(s) 6, 442
BmrFI CCNGG 1 cut(s) 936
BmsI GCATC 2 cut(s) 286, 448
BpmI CTGGAG 2 cut(s) 395, 738
Bpu14I TTCGAA 1 cut(s) 239
BpuEI CTTGAG 2 cut(s) 132, 420
BsaAI YACGTR 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 234
BsaWI WCCGGW 2 cut(s) 72, 213
BsaXI ACNNNNNCTCC 4 cut(s) 510, 540, 711, 741
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 764
Bse1I ACTGG 2 cut(s) 185, 524
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 1 cut(s) 936
BseGI GGATG 2 cut(s) 250, 301
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 33, 764
BseMII CTCAG 6 cut(s) 238, 248, 848, 863, 935, 938
BseNI ACTGG 2 cut(s) 185, 524
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 3 cut(s) 7, 10, 418
Bsh1236I CGCG 2 cut(s) 125, 165
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 77
BsiHKAI GWGCWC 1 cut(s) 371
BsiSI CCGG 2 cut(s) 73, 214
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 2 cut(s) 33, 764
BsmAI GTCTC 2 cut(s) 234, 736
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 1 cut(s) 168
Bso31I GGTCTC 1 cut(s) 234
Bsp119I TTCGAA 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 5 cut(s) 4, 625, 647, 658, 953
BspACI CCGC 7 cut(s) 68, 87, 96, 165, 194, 291, 973
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 6 cut(s) 237, 249, 847, 864, 934, 939
BspFNI CGCG 2 cut(s) 125, 165
BspLI GGNNCC 2 cut(s) 6, 442
BspPI GGATC 3 cut(s) 12, 633, 948
BspT104I TTCGAA 1 cut(s) 239
BspTI CTTAAG 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 234
BsrI ACTGG 2 cut(s) 185, 524
BssMI GATC 5 cut(s) 4, 625, 647, 658, 953
Bst2UI CCWGG 1 cut(s) 936
Bst6I CTCTTC 1 cut(s) 739
BstAFI CTTAAG 1 cut(s) 31
BstAPI GCANNNNNTGC 1 cut(s) 366
BstBAI YACGTR 1 cut(s) 330
BstBI TTCGAA 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 49, 371
BstDEI CTNAG 6 cut(s) 224, 257, 834, 872, 921, 947
BstENI CCTNNNNNAGG 1 cut(s) 31
BstF5I GGATG 2 cut(s) 250, 301
BstFNI CGCG 2 cut(s) 125, 165
BstHHI GCGC 1 cut(s) 830
BstKTI GATC 5 cut(s) 7, 628, 650, 661, 956
BstMAI GTCTC 2 cut(s) 234, 736
BstMBI GATC 5 cut(s) 4, 625, 647, 658, 953
BstMCI CGRYCG 1 cut(s) 77
BstMWI GCNNNNNNNGC 2 cut(s) 93, 366
BstNI CCWGG 1 cut(s) 936
BstSCI CCNGG 1 cut(s) 934
BstSFI CTRYAG 2 cut(s) 400, 417
BstSLI GKGCMC 1 cut(s) 371
BstUI CGCG 2 cut(s) 125, 165
BstV1I GCAGC 3 cut(s) 7, 10, 418
BstX2I RGATCY 3 cut(s) 4, 647, 953
BstYI RGATCY 3 cut(s) 4, 647, 953
BsuRI GGCC 1 cut(s) 168
BtsCI GGATG 2 cut(s) 250, 301
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 2 cut(s) 453, 615
Cac8I GCNNGC 2 cut(s) 49, 371
CfoI GCGC 1 cut(s) 830
Cfr13I GGNCC 5 cut(s) 70, 167, 182, 521, 788
CpoI CGGWCCG 1 cut(s) 70
CsiI ACCWGGT 1 cut(s) 934
Csp6I GTAC 4 cut(s) 327, 498, 593, 768
CspI CGGWCCG 1 cut(s) 70
CviAII CATG 3 cut(s) 515, 629, 696
CviQI GTAC 4 cut(s) 327, 498, 593, 768
DdeI CTNAG 6 cut(s) 224, 257, 834, 872, 921, 947
DpnI GATC 5 cut(s) 6, 627, 649, 660, 955
DpnII GATC 5 cut(s) 4, 625, 647, 658, 953
DriI GACNNNNNGTC 1 cut(s) 641
Eam1104I CTCTTC 1 cut(s) 739
Eam1105I GACNNNNNGTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 739
Eco31I GGTCTC 1 cut(s) 234
Eco47I GGWCC 4 cut(s) 70, 182, 521, 788
EcoNI CCTNNNNNAGG 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 934
FaeI CATG 3 cut(s) 518, 632, 699
FaqI GGGAC 1 cut(s) 547
FatI CATG 3 cut(s) 514, 628, 695
FauI CCCGC 2 cut(s) 89, 284
FauNDI CATATG 1 cut(s) 348
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 4 cut(s) 21, 24, 166, 432
FokI GGATG 2 cut(s) 257, 308
Fsp4HI GCNGC 4 cut(s) 21, 24, 166, 432
FspBI CTAG 2 cut(s) 450, 912
GlaI GCGC 1 cut(s) 829
GluI GCNGC 4 cut(s) 21, 24, 166, 432
GsuI CTGGAG 2 cut(s) 395, 738
HaeIII GGCC 1 cut(s) 168
HapII CCGG 2 cut(s) 73, 214
HhaI GCGC 1 cut(s) 830
Hin1II CATG 3 cut(s) 518, 632, 699
Hin6I GCGC 1 cut(s) 828
HinP1I GCGC 1 cut(s) 828
HinfI GANTC 7 cut(s) 139, 145, 303, 390, 481, 854, 908
HpaII CCGG 2 cut(s) 73, 214
HphI GGTGA 3 cut(s) 203, 755, 902
Hpy166II GTNNAC 4 cut(s) 279, 369, 400, 593
Hpy188I TCNGA 7 cut(s) 9, 144, 206, 258, 647, 837, 948
Hpy188III TCNNGA 1 cut(s) 581
Hpy8I GTNNAC 4 cut(s) 279, 369, 400, 593
HpyAV CCTTC 5 cut(s) 235, 562, 622, 702, 718
HpyCH4IV ACGT 2 cut(s) 198, 329
HpyCH4V TGCA 8 cut(s) 299, 352, 360, 369, 424, 492, 542, 678
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 366
HpyF3I CTNAG 6 cut(s) 224, 257, 834, 872, 921, 947
HpySE526I ACGT 2 cut(s) 198, 329
Hsp92II CATG 3 cut(s) 518, 632, 699
HspAI GCGC 1 cut(s) 828
Kzo9I GATC 5 cut(s) 4, 625, 647, 658, 953
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 3 cut(s) 7, 10, 418
LweI GCATC 2 cut(s) 286, 448
MabI ACCWGGT 1 cut(s) 934
MaeI CTAG 2 cut(s) 450, 912
MaeII ACGT 2 cut(s) 198, 329
MaeIII GTNAC 1 cut(s) 707
MalI GATC 5 cut(s) 6, 627, 649, 660, 955
MboI GATC 5 cut(s) 4, 625, 647, 658, 953
MboII GAAGA 5 cut(s) 245, 559, 726, 821, 963
MfeI CAATTG 1 cut(s) 353
MflI RGATCY 3 cut(s) 4, 647, 953
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 6 cut(s) 353, 666, 823, 885, 903, 964
MlyI GAGTC 2 cut(s) 133, 139
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 7 cut(s) 37, 173, 252, 396, 404, 512, 928
MseI TTAA 3 cut(s) 32, 174, 465
MslI CAYNNNNRTG 1 cut(s) 620
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 2 cut(s) 73, 214
MspR9I CCNGG 1 cut(s) 936
MunI CAATTG 1 cut(s) 353
MvaI CCWGG 1 cut(s) 936
MvnI CGCG 2 cut(s) 125, 165
MwoI GCNNNNNNNGC 2 cut(s) 93, 366
NdeI CATATG 1 cut(s) 348
NdeII GATC 5 cut(s) 4, 625, 647, 658, 953
NlaIII CATG 3 cut(s) 518, 632, 699
NlaIV GGNNCC 2 cut(s) 6, 442
NspV TTCGAA 1 cut(s) 239
OliI CACNNNNGTG 1 cut(s) 620
PfeI GAWTC 5 cut(s) 303, 390, 481, 854, 908
PflMI CCANNNNNTGG 1 cut(s) 764
PkrI GCNGC 4 cut(s) 22, 25, 167, 433
PleI GAGTC 2 cut(s) 133, 139
PpsI GAGTC 2 cut(s) 133, 139
Ppu21I YACGTR 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 934
PspGI CCWGG 1 cut(s) 934
PspN4I GGNNCC 2 cut(s) 6, 442
PspPI GGNCC 5 cut(s) 70, 167, 182, 521, 788
PsuI RGATCY 3 cut(s) 4, 647, 953
RsaI GTAC 4 cut(s) 328, 499, 594, 769
RsaNI GTAC 4 cut(s) 327, 498, 593, 768
RseI CAYNNNNRTG 1 cut(s) 620
Rsr2I CGGWCCG 1 cut(s) 70
RsrII CGGWCCG 1 cut(s) 70
SaqAI TTAA 3 cut(s) 32, 174, 465
SatI GCNGC 4 cut(s) 21, 24, 166, 432
Sau3AI GATC 5 cut(s) 4, 625, 647, 658, 953
Sau96I GGNCC 5 cut(s) 70, 167, 182, 521, 788
SchI GAGTC 2 cut(s) 133, 139
ScrFI CCNGG 1 cut(s) 936
SduI GDGCHC 1 cut(s) 371
SexAI ACCWGGT 1 cut(s) 934
SfaNI GCATC 2 cut(s) 286, 448
SfcI CTRYAG 2 cut(s) 400, 417
SfuI TTCGAA 1 cut(s) 239
SinI GGWCC 4 cut(s) 70, 182, 521, 788
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 3 cut(s) 31, 147, 435
SmoI CTYRAG 3 cut(s) 31, 147, 435
Sse9I AATT 6 cut(s) 353, 666, 823, 885, 903, 964
SsiI CCGC 7 cut(s) 68, 87, 96, 165, 194, 291, 973
SspMI CTAG 2 cut(s) 450, 912
StyD4I CCNGG 1 cut(s) 934
TaiI ACGT 2 cut(s) 201, 332
TaqI TCGA 5 cut(s) 77, 239, 284, 388, 961
TasI AATT 6 cut(s) 353, 666, 823, 885, 903, 964
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 5 cut(s) 303, 390, 481, 854, 908
Tru1I TTAA 3 cut(s) 32, 174, 465
Tru9I TTAA 3 cut(s) 32, 174, 465
TscAI CASTG 2 cut(s) 460, 622
TseI GCWGC 3 cut(s) 20, 23, 431
TspDTI ATGAA 2 cut(s) 326, 684
TspGWI ACGGA 2 cut(s) 128, 222
TspRI CASTG 2 cut(s) 460, 622
Van91I CCANNNNNTGG 1 cut(s) 764
Vha464I CTTAAG 1 cut(s) 31
VneI GTGCAC 1 cut(s) 367
VpaK11BI GGWCC 4 cut(s) 70, 182, 521, 788
XagI CCTNNNNNAGG 1 cut(s) 31
XapI RAATTY 1 cut(s) 964
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 2 cut(s) 450, 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.