Rmu_sc0004644.1_g000013

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004644.1
OG0005079
Physical Location & Seq
Reverse (-)
93657 .. 94775
1119 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 366 bp
ctggattacttctccactgaatgcatttattcaccaaatgcttatcgtggttttgctcgtgagttcatcaaagatttggaaagaatcagacctagggccatacttgacataatcaaatcaggggagaatttcagaatttctactgctacaaaaatgccggaacaggggacatgtgaacgctgtggttacatttcaagccagaaatggtgtaaagcttgtgtattgcttgagggactgaatcggggtttgccaaagatgggaattggtcggaatcgaggaattaacaacactcagaagaaggatatgaaacaaagtaatggaacaaaaagtatagagagcaaacaatgtgggactttggacttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

121

Amino Acids

13.74

Weight (kDa)

9.36

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zn-ribbon_14 PF16503 56 - 86 3.2e-19 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 127, 135
AfiI CCNNNNNNNGG 2 cut(s) 164, 257
AflIII ACRYGT 1 cut(s) 170
AgsI TTSAA 1 cut(s) 195
AluBI AGCT 1 cut(s) 215
AluI AGCT 1 cut(s) 215
AoxI GGCC 1 cut(s) 96
ApoI RAATTY 2 cut(s) 127, 135
AspA2I CCTAGG 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 24
AvrII CCTAGG 1 cut(s) 92
BauI CACGAG 1 cut(s) 57
BccI CCATC 1 cut(s) 250
BfaI CTAG 1 cut(s) 93
BlnI CCTAGG 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 248
BsaJI CCNNGG 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 257
BseDI CCNNGG 1 cut(s) 92
BseLI CCNNNNNNNGG 2 cut(s) 164, 257
BseMII CTCAG 1 cut(s) 305
BshFI GGCC 1 cut(s) 98
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 2 cut(s) 181, 246
BslI CCNNNNNNNGG 2 cut(s) 164, 257
BsmFI GGGAC 2 cut(s) 181, 246
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 1 cut(s) 98
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 92
BssSI CACGAG 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 92
Bst2BI CACGAG 1 cut(s) 57
BstDEI CTNAG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 174
BsuRI GGCC 1 cut(s) 98
BtsIMutI CAGTG 1 cut(s) 15
Cfr13I GGNCC 1 cut(s) 96
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 1 cut(s) 171
CviJI RGCY 3 cut(s) 98, 198, 215
CviKI_1 RGCY 3 cut(s) 98, 198, 215
DdeI CTNAG 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 92
EcoT22I ATGCAT 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 92
FaeI CATG 1 cut(s) 174
FaiI YATR 5 cut(s) 101, 110, 172, 305, 332
FaqI GGGAC 2 cut(s) 181, 246
FatI CATG 1 cut(s) 170
FspBI CTAG 1 cut(s) 93
HaeIII GGCC 1 cut(s) 98
HapII CCGG 1 cut(s) 158
Hin1II CATG 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 213
HinfI GANTC 3 cut(s) 84, 238, 271
HpaII CCGG 1 cut(s) 158
HphI GGTGA 1 cut(s) 24
Hpy166II GTNNAC 1 cut(s) 176
Hpy188I TCNGA 5 cut(s) 89, 134, 270, 294, 365
Hpy188III TCNNGA 1 cut(s) 59
Hpy8I GTNNAC 1 cut(s) 176
HpyAV CCTTC 1 cut(s) 292
HpyCH4V TGCA 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 291
Hsp92II CATG 1 cut(s) 174
LpnPI CCDG 4 cut(s) 105, 149, 171, 212
MaeI CTAG 1 cut(s) 93
MaeIII GTNAC 1 cut(s) 185
MboII GAAGA 1 cut(s) 307
MluCI AATT 4 cut(s) 127, 135, 261, 279
MmeI TCCRAC 1 cut(s) 248
MnlI CCTC 2 cut(s) 223, 269
Mph1103I ATGCAT 1 cut(s) 26
MseI TTAA 1 cut(s) 282
MspI CCGG 1 cut(s) 158
Mva1269I GAATGC 1 cut(s) 26
NlaIII CATG 1 cut(s) 174
NsiI ATGCAT 1 cut(s) 26
NspI RCATGY 1 cut(s) 174
PciI ACATGT 1 cut(s) 170
PctI GAATGC 1 cut(s) 26
PfeI GAWTC 3 cut(s) 84, 238, 271
PscI ACATGT 1 cut(s) 170
PspPI GGNCC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 282
Sau96I GGNCC 1 cut(s) 96
SetI ASST 2 cut(s) 94, 217
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 4 cut(s) 127, 135, 261, 279
SspMI CTAG 1 cut(s) 93
StyI CCWWGG 1 cut(s) 92
TaqI TCGA 1 cut(s) 274
TasI AATT 4 cut(s) 127, 135, 261, 279
TfiI GAWTC 3 cut(s) 84, 238, 271
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 22
TspDTI ATGAA 2 cut(s) 55, 320
TspRI CASTG 1 cut(s) 22
XapI RAATTY 2 cut(s) 127, 135
XceI RCATGY 1 cut(s) 174
XmaJI CCTAGG 1 cut(s) 92
XspI CTAG 1 cut(s) 93
Zsp2I ATGCAT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.