Rmu_sc0004728.1_g000005

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004728.1
OG0002639
Physical Location & Seq
Forward (+)
18116 .. 25796
7681 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2583 bp
atggaaacccacagctcctgtagcatcacaaacgacgacggcgaatggagagccgaaaaagctataggcggcaacgcccaagctctccaagctctccgagaactcatcgtctaccctctcctcttttctcgccaagccaaaaagctcggtctcaaatggccccgaggtttgcttctctacggtcctcccggcaccggaaagacaagcttggtccgcgcagtggttcaggaatgtggtgcgcatttagttctcatcagtccgcatactgttcacagagcttataccggagagagtgagttggcactgcgagaggcttttgcggaggccgcgtcacataccttgtcagggaagccgtctgttgtgtttatagatgaaatcgatgccctctgtcctcgccgtgattctagttttagctcgccgcggatatcatcgccgcctccgcctcagaagcagtcgctgctctatgcttcaatggctcacggacggctcaataactccaccagcggccttaaacgcagcttctccgacgagaatcgtaagcccgacgcctttgccaagaggcagaagctttcgcagcacaggaaggctctcccgatcgcttctgttgagaagcgtctcgtagaggaagttagaaagcatgatactttgattattgttggcgaaactggaagcggaaaaacaacacaattacctcagtttttgtacaatgctggattttgtgggtatgggaaagtcattggggtaactcagcctaggcgtgtggctgctatgactgtggcgaaacgggttgctgaggaatgcggtgttactttgggtgaaaaggttggttacgctgtcagatttgaggatgtgacctctagttcaacaaggatcaagtatatgactgatggattgctactgagggaagcactattggatccatatctctctaagtattcagtcgtcattgtcgatgaagcgcatgagagaactgtccagactgatgtcttactgggcttgcttaaaaatgtacaaaatgcaaggtcaaaagttgtcaataaccaccaagatacaaaaaacacgaagacaaataatgacatgcagttggataaagaaaatggttctcattccttgagctttctcaagcaatgccaaggaaggaatccattgaagttaattatcatgtctgccagtttggatgcacgaattttctccgagtacttcggtggtgcaagagctgttcacatccaaggtcgacagtttcctgttgatatatattatactcatcacaatgtacaagattatctagatgcatcgttaactgccatatttcagattcatatggaggaggaccctggtgatatacttgtatttttgactggtcaagaagaaattgaatctgtacagagacttgtcaaagaaagacttaaacagttacctgaaaccagtcaaaaacttgtagctgttcctatattctcatctcttccatcagaacagcaaatgcaagtgtttgcaccggctcctgatggatttcgtaaggtaatattggcaacgaatattgccgagacatctgtgacaatacctggaatcaagtatgtaatagatcctgggtttgtaaaagcacgtacctatgatccgaataaagggttatcaagtttgaatgttattccaatttcaaaagcacaggctcttcagagaagtgggcgtgcagggagagaaagatcagggaagtgcttccgcctctacccagagaatcaatttgagaaacttgaggattcaacaaagccagagatcaagcggtgtgacctctctagtataattttgcaacttaaggttctgggtgttgatgatgtcattgggttcgacttcatagataaaccttcaaggtcggctattgtcaaatcactggagcaattgtatgtgctaggtgctttaacagatgagtgtaaactatcagatccagttggacgtcaaatggcaagccttcccctagaccctattttctcaaaagctgtaatcctagcaagtgaaactgaattcaattgcctcgaagagatgttgataactgttgcaatgctctcagttgagtccatatttttcgctccccgtgagaaattgaacgaggctagaactgcaatgaaatgctttgcaagtccagatggggaccacctcactttggttaatgtatatcgagcgtttaatgagttttcggagaagagatcagatctcagtaaagagaaacatgaaaaaagtctcaggaaatggtgcaaggacaatttcattaatggtcgctccctgaagcatgctcgtgacattcacagccaaatccgagggaatgttgaacaaatgggtcttcatgtagcttcttgcggggatgacatgcttccgtttcgaagatgtcttgcagcgtctttcttcctcaatgcagctttaaagcagcccgagggaacttacagggctttagcaaatggtcaggtggttcggattcatccaattgaatctagtgccaaaaggtcttggatagatttgatcatttccaattgcaagttacatgcaagcattatcagatatttcccacctcatcaactgggtcgaaaaccctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0001817 GO:0001819 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003723 GO:0003724 GO:0003725 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006356 GO:0006396 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0008026 GO:0008134 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032479 GO:0032481 GO:0032991 GO:0033613 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042623 GO:0043021 GO:0043023 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043408 GO:0043410 GO:0043484 GO:0043565 GO:0043603 GO:0043604 GO:0044087 GO:0044089 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045937 GO:0045943 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051092 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0060255 GO:0061702 GO:0065007 GO:0065009 GO:0070013 GO:0070035 GO:0071704 GO:0072559 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:1900225 GO:1900227 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 ko03050 ko05169 map03040 map03050 map05169
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

860

Amino Acids

95.78

Weight (kDa)

9.06

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 57 - 135 1.8e-21 ATPase family associated with various cellular activities (AAA)
Helicase_C PF00271 436 - 565 4.7e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 628 - 657 2.6e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 658 - 719 4.1e-11 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1951
Acc16I TGCGCA 1 cut(s) 240
AccB1I GGYRCC 1 cut(s) 191
AccI GTMKAC 2 cut(s) 111, 1234
AccII CGCG 3 cut(s) 216, 329, 421
AclWI GGATC 6 cut(s) 878, 911, 924, 1577, 1607, 1931
AcsI RAATTY 2 cut(s) 1185, 2015
AcuI CTGAAG 2 cut(s) 1655, 2297
AcyI GRCGYC 2 cut(s) 546, 1948
AfaI GTAC 6 cut(s) 704, 1011, 1199, 1275, 1383, 1606
AfiI CCNNNNNNNGG 5 cut(s) 194, 220, 345, 1622, 2155
AflII CTTAAG 1 cut(s) 1808
AjnI CCWGG 3 cut(s) 1333, 1561, 1585
Alw26I GTCTC 5 cut(s) 155, 620, 1381, 1538, 2237
AlwI GGATC 6 cut(s) 878, 911, 924, 1577, 1607, 1931
AlwNI CAGNNNCTG 2 cut(s) 18, 457
Ama87I CYCGRG 2 cut(s) 162, 2420
AoxI GGCC 3 cut(s) 158, 324, 505
ApeKI GCWGC 7 cut(s) 457, 516, 574, 764, 2384, 2405, 2416
ApoI RAATTY 2 cut(s) 1185, 2015
AseI ATTAAT 1 cut(s) 2262
Asp700I GAANNNNTTC 1 cut(s) 1712
AspA2I CCTAGG 1 cut(s) 752
AspLEI GCGC 3 cut(s) 218, 241, 961
AspS9I GGNCC 5 cut(s) 159, 182, 211, 1330, 2143
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 2 cut(s) 827, 1349
AsuII TTCGAA 1 cut(s) 2371
AvaI CYCGRG 2 cut(s) 162, 2420
AvaII GGWCC 4 cut(s) 182, 211, 1330, 2143
AvrII CCTAGG 1 cut(s) 752
BamHI GGATCC 1 cut(s) 916
BanI GGYRCC 1 cut(s) 191
BauI CACGAG 1 cut(s) 2286
BbsI GAAGAC 2 cut(s) 1070, 2324
BbvCI CCTCAGC 1 cut(s) 792
BbvI GCAGC 7 cut(s) 444, 528, 586, 751, 2396, 2417, 2428
BccI CCATC 4 cut(s) 881, 1474, 1499, 2132
BceAI ACGGC 4 cut(s) 55, 337, 381, 500
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 3 cut(s) 1335, 1563, 1587
BclI TGATCA 1 cut(s) 2508
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 5 cut(s) 155, 620, 1381, 1538, 2237
BfmI CTRYAG 2 cut(s) 19, 63
BfrI CTTAAG 1 cut(s) 1808
BglII AGATCT 1 cut(s) 2203
BlnI CCTAGG 1 cut(s) 752
BmcAI AGTACT 1 cut(s) 1199
Bme1390I CCNGG 4 cut(s) 189, 1335, 1563, 1587
Bme18I GGWCC 4 cut(s) 182, 211, 1330, 2143
BmeT110I CYCGRG 2 cut(s) 162, 2420
BmgT120I GGNCC 5 cut(s) 159, 182, 211, 1330, 2143
BmiI GGNNCC 6 cut(s) 161, 193, 918, 1332, 1500, 2144
BmrFI CCNGG 4 cut(s) 189, 1335, 1563, 1587
BmrI ACTGGG 2 cut(s) 1001, 2576
BmsI GCATC 5 cut(s) 33, 370, 1168, 1279, 1301
BmuI ACTGGG 2 cut(s) 1001, 2576
BoxI GACNNNNGTC 1 cut(s) 983
BpiI GAAGAC 2 cut(s) 1070, 2324
BpmI CTGGAG 1 cut(s) 1907
Bpu10I CCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 2371
BpuEI CTTGAG 3 cut(s) 1106, 1132, 1769
BpuMI CCSGG 1 cut(s) 189
Bsa29I ATCGAT 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 1604
BsaBI GATNNNNATC 1 cut(s) 921
BsaHI GRCGYC 2 cut(s) 546, 1948
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 9 cut(s) 163, 419, 752, 1132, 1228, 1333, 1586, 2308, 2421
BsaWI WCCGGW 2 cut(s) 194, 284
BsaXI ACNNNNNCTCC 4 cut(s) 314, 344, 1880, 1910
Bsc4I CCNNNNNNNGG 5 cut(s) 194, 220, 345, 1622, 2155
Bse118I RCCGGY 1 cut(s) 1495
Bse1I ACTGG 8 cut(s) 670, 996, 1170, 1363, 1425, 1890, 1940, 2571
Bse3DI GCAATG 3 cut(s) 1133, 2058, 2121
Bse8I GATNNNNATC 1 cut(s) 921
BseBI CCWGG 3 cut(s) 1335, 1563, 1587
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 9 cut(s) 163, 419, 752, 1132, 1228, 1333, 1586, 2308, 2421
BseGI GGATG 5 cut(s) 853, 1183, 1224, 2359, 2467
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 5 cut(s) 194, 220, 345, 1622, 2155
BseMI GCAATG 3 cut(s) 1133, 2058, 2121
BseMII CTCAG 8 cut(s) 458, 707, 761, 783, 890, 2073, 2221, 2248
BseNI ACTGG 8 cut(s) 670, 996, 1170, 1363, 1425, 1890, 1940, 2571
BseRI GAGGAG 2 cut(s) 110, 1340
BseXI GCAGC 7 cut(s) 444, 528, 586, 751, 2396, 2417, 2428
BsgI GTGCAG 1 cut(s) 1707
Bsh1236I CGCG 3 cut(s) 216, 329, 421
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 3 cut(s) 160, 326, 507
BshNI GGYRCC 1 cut(s) 191
BshVI ATCGAT 1 cut(s) 378
BsiEI CGRYCG 1 cut(s) 597
BsiHKCI CYCGRG 2 cut(s) 162, 2420
BsiSI CCGG 4 cut(s) 189, 195, 285, 1496
BslFI GGGAC 1 cut(s) 2156
BslI CCNNNNNNNGG 5 cut(s) 194, 220, 345, 1622, 2155
BsmAI GTCTC 5 cut(s) 155, 620, 1381, 1538, 2237
BsmBI CGTCTC 1 cut(s) 620
BsmFI GGGAC 1 cut(s) 2156
BsmI GAATGC 1 cut(s) 803
BsnI GGCC 3 cut(s) 160, 326, 507
Bso31I GGTCTC 1 cut(s) 155
BsoBI CYCGRG 2 cut(s) 162, 2420
Bsp119I TTCGAA 1 cut(s) 2371
Bsp1407I TGTACA 4 cut(s) 702, 1009, 1273, 1381
BspANI GGCC 3 cut(s) 160, 326, 507
BspCNI CTCAG 8 cut(s) 457, 706, 760, 784, 891, 2072, 2220, 2247
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 3 cut(s) 216, 329, 421
BspLI GGNNCC 6 cut(s) 161, 193, 918, 1332, 1500, 2144
BspPI GGATC 6 cut(s) 878, 911, 924, 1577, 1607, 1931
BspQI GCTCTTC 1 cut(s) 1674
BspT104I TTCGAA 1 cut(s) 2371
BspT107I GGYRCC 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 1808
BspTNI GGTCTC 1 cut(s) 155
BsrDI GCAATG 3 cut(s) 1133, 2058, 2121
BsrFI RCCGGY 1 cut(s) 1495
BsrGI TGTACA 4 cut(s) 702, 1009, 1273, 1381
BsrI ACTGG 8 cut(s) 670, 996, 1170, 1363, 1425, 1890, 1940, 2571
BssAI RCCGGY 1 cut(s) 1495
BssECI CCNNGG 9 cut(s) 163, 419, 752, 1132, 1228, 1333, 1586, 2308, 2421
BssNI GRCGYC 2 cut(s) 546, 1948
BssSI CACGAG 1 cut(s) 2286
BssT1I CCWWGG 3 cut(s) 752, 1132, 1228
Bst2BI CACGAG 1 cut(s) 2286
Bst2UI CCWGG 3 cut(s) 1335, 1563, 1587
Bst4CI ACNGT 7 cut(s) 182, 268, 775, 973, 1239, 1413, 2047
Bst6I CTCTTC 4 cut(s) 1467, 1674, 2025, 2189
BstACI GRCGYC 2 cut(s) 546, 1948
BstAFI CTTAAG 1 cut(s) 1808
BstAPI GCANNNNNTGC 1 cut(s) 457
BstAUI TGTACA 4 cut(s) 702, 1009, 1273, 1381
BstBAI YACGTR 1 cut(s) 1604
BstBI TTCGAA 1 cut(s) 2371
BstC8I GCNNGC 6 cut(s) 416, 998, 1686, 1960, 2283, 2536
BstDEI CTNAG 9 cut(s) 444, 693, 747, 792, 899, 930, 2059, 2207, 2234
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 5 cut(s) 853, 1183, 1224, 2359, 2467
BstFNI CGCG 3 cut(s) 216, 329, 421
BstHHI GCGC 3 cut(s) 218, 241, 961
BstMAI GTCTC 5 cut(s) 155, 620, 1381, 1538, 2237
BstMCI CGRYCG 1 cut(s) 597
BstNI CCWGG 3 cut(s) 1335, 1563, 1587
BstNSI RCATGY 4 cut(s) 1081, 2285, 2362, 2534
BstPAI GACNNNNGTC 1 cut(s) 983
BstSCI CCNGG 4 cut(s) 187, 1333, 1561, 1585
BstSFI CTRYAG 2 cut(s) 19, 63
BstUI CGCG 3 cut(s) 216, 329, 421
BstV1I GCAGC 7 cut(s) 444, 528, 586, 751, 2396, 2417, 2428
BstV2I GAAGAC 2 cut(s) 1070, 2324
BstX2I RGATCY 4 cut(s) 916, 1582, 1936, 2203
BstYI RGATCY 4 cut(s) 916, 1582, 1936, 2203
Bsu15I ATCGAT 1 cut(s) 378
BsuRI GGCC 3 cut(s) 160, 326, 507
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 1 cut(s) 419
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 5 cut(s) 853, 1183, 1224, 2359, 2467
BtsI GCAGTG 2 cut(s) 225, 302
BtsIMutI CAGTG 3 cut(s) 225, 302, 1883
Cac8I GCNNGC 6 cut(s) 416, 998, 1686, 1960, 2283, 2536
CaiI CAGNNNCTG 2 cut(s) 18, 457
CfoI GCGC 3 cut(s) 218, 241, 961
Cfr10I RCCGGY 1 cut(s) 1495
Cfr13I GGNCC 5 cut(s) 159, 182, 211, 1330, 2143
Cfr42I CCGCGG 1 cut(s) 422
ClaI ATCGAT 1 cut(s) 378
CseI GACGC 4 cut(s) 318, 554, 602, 2376
Csp6I GTAC 6 cut(s) 703, 1010, 1198, 1274, 1382, 1605
CviAII CATG 9 cut(s) 638, 962, 1078, 1162, 2222, 2282, 2336, 2359, 2531
CviQI GTAC 6 cut(s) 703, 1010, 1198, 1274, 1382, 1605
DdeI CTNAG 9 cut(s) 444, 693, 747, 792, 899, 930, 2059, 2207, 2234
DraI TTTAAA 1 cut(s) 2412
Eam1104I CTCTTC 4 cut(s) 1467, 1674, 2025, 2189
EarI CTCTTC 4 cut(s) 1467, 1674, 2025, 2189
EciI GGCGGA 2 cut(s) 429, 1706
Eco130I CCWWGG 3 cut(s) 752, 1132, 1228
Eco31I GGTCTC 1 cut(s) 155
Eco32I GATATC 1 cut(s) 426
Eco47I GGWCC 4 cut(s) 182, 211, 1330, 2143
Eco57I CTGAAG 2 cut(s) 1655, 2297
Eco88I CYCGRG 2 cut(s) 162, 2420
EcoO109I RGGNCCY 1 cut(s) 1330
EcoRI GAATTC 1 cut(s) 2015
EcoRII CCWGG 3 cut(s) 1333, 1561, 1585
EcoRV GATATC 1 cut(s) 426
EcoT14I CCWWGG 3 cut(s) 752, 1132, 1228
EcoT22I ATGCAT 1 cut(s) 1294
ErhI CCWWGG 3 cut(s) 752, 1132, 1228
Esp3I CGTCTC 1 cut(s) 620
FaeI CATG 9 cut(s) 641, 965, 1081, 1165, 2225, 2285, 2339, 2362, 2534
FalI AAGNNNNNCTT 4 cut(s) 191, 223, 1389, 1421
FaqI GGGAC 1 cut(s) 2156
FatI CATG 9 cut(s) 637, 961, 1077, 1161, 2221, 2281, 2335, 2358, 2530
FauI CCCGC 1 cut(s) 2342
FauNDI CATATG 1 cut(s) 1320
FbaI TGATCA 1 cut(s) 2508
FblI GTMKAC 2 cut(s) 111, 1234
FokI GGATG 5 cut(s) 860, 1190, 1211, 2366, 2454
FspAI RTGCGCAY 1 cut(s) 240
FspI TGCGCA 1 cut(s) 240
GlaI GCGC 3 cut(s) 217, 240, 960
GsuI CTGGAG 1 cut(s) 1907
HaeIII GGCC 3 cut(s) 160, 326, 507
HapII CCGG 4 cut(s) 189, 195, 285, 1496
HgaI GACGC 4 cut(s) 318, 554, 602, 2376
HhaI GCGC 3 cut(s) 218, 241, 961
Hin1I GRCGYC 2 cut(s) 546, 1948
Hin1II CATG 9 cut(s) 641, 965, 1081, 1165, 2225, 2285, 2339, 2362, 2534
Hin6I GCGC 3 cut(s) 216, 239, 959
HinP1I GCGC 3 cut(s) 216, 239, 959
HincII GTYRAC 2 cut(s) 1235, 1299
HindII GTYRAC 2 cut(s) 1235, 1299
HindIII AAGCTT 2 cut(s) 205, 566
HpaI GTTAAC 1 cut(s) 1299
HpaII CCGG 4 cut(s) 189, 195, 285, 1496
HphI GGTGA 2 cut(s) 827, 1349
Hpy166II GTNNAC 6 cut(s) 112, 271, 1222, 1235, 1299, 1928
Hpy188III TCNNGA 9 cut(s) 227, 592, 976, 1286, 1364, 1502, 2135, 2236, 2288
Hpy8I GTNNAC 6 cut(s) 112, 271, 1222, 1235, 1299, 1928
Hpy99I CGWCG 4 cut(s) 38, 41, 530, 548
HpyAV CCTTC 4 cut(s) 577, 1131, 1869, 1973
HpyCH4III ACNGT 7 cut(s) 182, 268, 775, 973, 1239, 1413, 2047
HpyCH4IV ACGT 2 cut(s) 1603, 1948
HpyF3I CTNAG 9 cut(s) 444, 693, 747, 792, 899, 930, 2059, 2207, 2234
HpySE526I ACGT 2 cut(s) 1603, 1948
Hsp92I GRCGYC 2 cut(s) 546, 1948
Hsp92II CATG 9 cut(s) 641, 965, 1081, 1165, 2225, 2285, 2339, 2362, 2534
HspAI GCGC 3 cut(s) 216, 239, 959
Ksp22I TGATCA 1 cut(s) 2508
KspAI GTTAAC 1 cut(s) 1299
KspI CCGCGG 1 cut(s) 422
LguI GCTCTTC 1 cut(s) 1674
LmnI GCTCC 5 cut(s) 20, 1504, 1888, 2086, 2276
Lsp1109I GCAGC 7 cut(s) 444, 528, 586, 751, 2396, 2417, 2428
LweI GCATC 5 cut(s) 33, 370, 1168, 1279, 1301
MaeII ACGT 2 cut(s) 1603, 1948
MboII GAAGA 9 cut(s) 1075, 1379, 1454, 1661, 2042, 2206, 2324, 2385, 2386
MfeI CAATTG 4 cut(s) 1892, 2020, 2472, 2518
MflI RGATCY 4 cut(s) 916, 1582, 1936, 2203
MlyI GAGTC 1 cut(s) 2075
MmeI TCCRAC 3 cut(s) 549, 1065, 1924
Mph1103I ATGCAT 1 cut(s) 1294
MroXI GAANNNNTTC 1 cut(s) 1712
MslI CAYNNNNRTG 2 cut(s) 1269, 2286
MspA1I CMGCKG 2 cut(s) 421, 504
MspCI CTTAAG 1 cut(s) 1808
MspI CCGG 4 cut(s) 189, 195, 285, 1496
MspR9I CCNGG 4 cut(s) 189, 1335, 1563, 1587
MunI CAATTG 4 cut(s) 1892, 2020, 2472, 2518
Mva1269I GAATGC 1 cut(s) 803
MvaI CCWGG 3 cut(s) 1335, 1563, 1587
MvnI CGCG 3 cut(s) 216, 329, 421
NciI CCSGG 1 cut(s) 189
NdeI CATATG 1 cut(s) 1320
NlaIII CATG 9 cut(s) 641, 965, 1081, 1165, 2225, 2285, 2339, 2362, 2534
NlaIV GGNNCC 6 cut(s) 161, 193, 918, 1332, 1500, 2144
NmeAIII GCCGAG 1 cut(s) 1567
NmuCI GTSAC 5 cut(s) 330, 850, 1552, 1781, 2288
NsbI TGCGCA 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 1294
NspI RCATGY 4 cut(s) 1081, 2285, 2362, 2534
NspV TTCGAA 1 cut(s) 2371
PaeI GCATGC 1 cut(s) 2285
PciSI GCTCTTC 1 cut(s) 1674
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 948
PctI GAATGC 1 cut(s) 803
PdmI GAANNNNTTC 1 cut(s) 1712
Ple19I CGATCG 1 cut(s) 597
PleI GAGTC 1 cut(s) 2074
PpsI GAGTC 1 cut(s) 2074
Ppu21I YACGTR 1 cut(s) 1604
PpuMI RGGWCCY 1 cut(s) 1330
PshAI GACNNNNGTC 1 cut(s) 983
PshBI ATTAAT 1 cut(s) 2262
Psp5II RGGWCCY 1 cut(s) 1330
Psp6I CCWGG 3 cut(s) 1333, 1561, 1585
PspGI CCWGG 3 cut(s) 1333, 1561, 1585
PspN4I GGNNCC 6 cut(s) 161, 193, 918, 1332, 1500, 2144
PspPI GGNCC 5 cut(s) 159, 182, 211, 1330, 2143
PspPPI RGGWCCY 1 cut(s) 1330
PstNI CAGNNNCTG 2 cut(s) 18, 457
PsuI RGATCY 4 cut(s) 916, 1582, 1936, 2203
PvuI CGATCG 1 cut(s) 597
RsaI GTAC 6 cut(s) 704, 1011, 1199, 1275, 1383, 1606
RsaNI GTAC 6 cut(s) 703, 1010, 1198, 1274, 1382, 1605
RseI CAYNNNNRTG 2 cut(s) 1269, 2286
SacII CCGCGG 1 cut(s) 422
SalI GTCGAC 1 cut(s) 1233
SapI GCTCTTC 1 cut(s) 1674
Sau96I GGNCC 5 cut(s) 159, 182, 211, 1330, 2143
ScaI AGTACT 1 cut(s) 1199
SchI GAGTC 1 cut(s) 2075
ScrFI CCNGG 4 cut(s) 189, 1335, 1563, 1587
SfaNI GCATC 5 cut(s) 33, 370, 1168, 1279, 1301
SfcI CTRYAG 2 cut(s) 19, 63
Sfr303I CCGCGG 1 cut(s) 422
SfuI TTCGAA 1 cut(s) 2371
SgrBI CCGCGG 1 cut(s) 422
SinI GGWCC 4 cut(s) 182, 211, 1330, 2143
SmiMI CAYNNNNRTG 2 cut(s) 1269, 2286
SmlI CTYRAG 4 cut(s) 1111, 1121, 1748, 1808
SmoI CTYRAG 4 cut(s) 1111, 1121, 1748, 1808
SphI GCATGC 1 cut(s) 2285
SspI AATATT 2 cut(s) 1524, 1537
StyD4I CCNGG 4 cut(s) 187, 1333, 1561, 1585
StyI CCWWGG 3 cut(s) 752, 1132, 1228
TaaI ACNGT 7 cut(s) 182, 268, 775, 973, 1239, 1413, 2047
TaiI ACGT 2 cut(s) 1606, 1951
TaqI TCGA 8 cut(s) 378, 951, 1234, 1842, 2028, 2170, 2371, 2572
TaqII GACCGA 1 cut(s) 137
TatI WGTACW 5 cut(s) 702, 1009, 1197, 1273, 1381
TauI GCSGC 5 cut(s) 72, 329, 421, 436, 507
TscAI CASTG 3 cut(s) 225, 309, 1890
TseFI GTSAC 5 cut(s) 330, 850, 1552, 1781, 2288
TseI GCWGC 7 cut(s) 457, 516, 574, 764, 2384, 2405, 2416
Tsp45I GTSAC 5 cut(s) 330, 850, 1552, 1781, 2288
TspDTI ATGAA 9 cut(s) 387, 969, 1307, 1837, 2132, 2238, 2248, 2324, 2456
TspGWI ACGGA 2 cut(s) 495, 2355
TspRI CASTG 3 cut(s) 225, 309, 1890
Vha464I CTTAAG 1 cut(s) 1808
VpaK11BI GGWCC 4 cut(s) 182, 211, 1330, 2143
VspI ATTAAT 1 cut(s) 2262
XapI RAATTY 2 cut(s) 1185, 2015
XbaI TCTAGA 1 cut(s) 1285
XceI RCATGY 4 cut(s) 1081, 2285, 2362, 2534
XmaJI CCTAGG 1 cut(s) 752
XmiI GTMKAC 2 cut(s) 111, 1234
XmnI GAANNNNTTC 1 cut(s) 1712
ZraI GACGTC 1 cut(s) 1949
ZrmI AGTACT 1 cut(s) 1199
Zsp2I ATGCAT 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.