Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005592.1

Orthogroup ID

OG0013752

Physical Location & Seq

Genomic Coordinates Forward (+)

73750 .. 77935

Gene Structure 4186 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0005592.1_g000022.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1572 bp

atggcttcggcgtggcggcttgttgctgagtggcggcgatttggtggcgttggagcttgccggaggtgggtgtggctgcttgcagcgcgtggcttctgtgaggggtggggagaatgggccttggactgggctaatgtatttgggcctcgttgcatttttgtaattttcaatttggtttgttattgtttttttctagtggttgcttggttaaaaataagtcccccctctgttgggcgggggtgtgggtgtgtccggtctgagtcgatgccgttgttctggtgtcatggtcgttttgtagttccatctctatggcttagtctgttgactatgctgcctgttcttcaacgtgacgaggaattacccttacacgtggtctgcttattttgggacacggtgtcggaaagggtgatgcgattcttcgtcaggttgggtgcagggttgtatctttgcactagagtttggttactgctctctgtagcccgcttgaatgggcgtaattggtggtcggggtttggacttgtttggttcatgaaggtcatttcatatgacctccttgtcacctatggtgtttctgaggttgtgcgcgacccatcaatcattcgatctgtggatgagggcagagaagggttttgggggttgtcagctagcctgggcagagaaggagaaggatccacctgtcattggagggccaccaaactgcaaaactctaagggcagagatggtcgtattaggttccatgcatttggctccagaatgagtaatcatgccgcattgagaacacctacctttgagatgctcaaagtgaaacttcaagattggttcaatcccgttctccgatcctctgtcgtgatcgacttctgcatcatgaagcccttttacgccggcacaacactcctctggcacgctccctccaaacactccaaaagggatgcatggatacgtggaatcaaagtcacaaattctaacggtattgattgtgaggattttgattatactacgtctacattgagcttcttcatgcttccttccatggctacatctgagagcttcttctccatctttgctcttctgggtttcggtaaagtcgtcttctttaatggtttcagagctttggggacatcatctaagagctccctgacaaaccatggctgggcagccaagtattatcctttcacagttatccctcagatcttgatgatggaaaagaaagggaagagcagtaaaagtagagagtcagagtttgtgcttcaatgggggaacaagaagacgctgagatatcagaaggtgagggtcaagaaagaacgaagctttgacaacaacaccaaatccaggaaaatcagagactctccctctccactaccacagcgcatcaacaagagctcaccgtcgccaatgaatagtagaattaaagttatttatcacatcgtcggagaaggaagatcggtattttccttgggtttagatgataatggtaagatgttaggattagatcatcatggtcatgcagtggaggataaaggaagcaatgtttggccaaaattgttcccatcattgtctagtaaagaaatataa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1639

Protein Analysis

523

Amino Acids

59.65

Weight (kDa)

9.84

Isoelectric Point (pI)

43.38

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013752)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr1g0363721 RchiOBHm_Chr2g0173981 RchiOBHm_Chr4g0394391
rosa_multiflora	Rmu_sc0001644.1_g000025 Rmu_sc0003301.1_g000014 Rmu_sc0005592.1_g000022 Rmu_sc0009451.1_g000005 Rmu_sc0033532.1_g000001
rosa_roxburghii	Rroxscaffold_160G00433970 Rroxscaffold_1G00043050 Rroxscaffold_4G00311030 Rroxscaffold_5G00368050 Rroxscaffold_6G00395850 Rroxscaffold_6G00404020 Rroxscaffold_7G00182210
rosa_rugosa	Rorug05G0195200
rosa_samantha	Rh4DG298400
rosa_wichuraiana	Rw4G008430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	554
AccI	GTMKAC	1 cut(s)	1001
AccII	CGCG	2 cut(s)	88, 585
AciI	CCGC	5 cut(s)	16, 34, 235, 481, 768
AclWI	GGATC	3 cut(s)	663, 676, 831
AcoI	YGGCCR	1 cut(s)	1532
AcsI	RAATTY	1 cut(s)	958
AcvI	CACGTG	1 cut(s)	370
AfiI	CCNNNNNNNGG	7 cut(s)	67, 126, 230, 231, 681, 1150, 1329
AflIII	ACRYGT	1 cut(s)	367
AgsI	TTSAA	6 cut(s)	169, 344, 487, 812, 823, 1250
AhdI	GACNNNNNGTC	1 cut(s)	394
AjnI	CCWGG	2 cut(s)	648, 1328
AjuI	GAANNNNNNNTTGG	2 cut(s)	1513, 1545
AluBI	AGCT	8 cut(s)	56, 644, 1011, 1047, 1109, 1131, 1308, 1380
AluI	AGCT	8 cut(s)	56, 644, 1011, 1047, 1109, 1131, 1308, 1380
Alw21I	GWGCWC	2 cut(s)	1133, 1382
Alw26I	GTCTC	1 cut(s)	1335
AlwI	GGATC	3 cut(s)	663, 676, 831
AoxI	GGCC	4 cut(s)	117, 143, 687, 1532
ApeKI	GCWGC	4 cut(s)	76, 83, 331, 1154
ApoI	RAATTY	1 cut(s)	958
AspLEI	GCGC	3 cut(s)	88, 585, 1368
AspS9I	GGNCC	3 cut(s)	117, 143, 687
AsuHPI	GGTGA	4 cut(s)	418, 550, 1297, 1374
AsuNHI	GCTAGC	1 cut(s)	644
BalI	TGGCCA	1 cut(s)	1534
BamHI	GGATCC	1 cut(s)	668
BanII	GRGCYC	2 cut(s)	1133, 1382
BarI	GAAGNNNNNNTAC	2 cut(s)	860, 892
BbrPI	CACGTG	1 cut(s)	370
BbsI	GAAGAC	2 cut(s)	1081, 1271
Bbv12I	GWGCWC	2 cut(s)	1133, 1382
BbvI	GCAGC	4 cut(s)	63, 95, 318, 1166
BccI	CCATC	6 cut(s)	310, 598, 713, 1064, 1192, 1555
BceAI	ACGGC	1 cut(s)	253
BciT130I	CCWGG	2 cut(s)	650, 1330
BciVI	GTATCC	1 cut(s)	930
BcoDI	GTCTC	1 cut(s)	1335
BfaI	CTAG	4 cut(s)	194, 453, 645, 1557
BfmI	CTRYAG	1 cut(s)	474
BfuI	GTATCC	1 cut(s)	930
BglII	AGATCT	1 cut(s)	1188
BisI	GCNGC	7 cut(s)	17, 35, 77, 84, 332, 768, 1155
BlsI	GCNGC	7 cut(s)	18, 36, 78, 85, 333, 769, 1156
Bme1390I	CCNGG	2 cut(s)	650, 1330
BmeRI	GACNNNNNGTC	1 cut(s)	394
BmgT120I	GGNCC	3 cut(s)	117, 143, 687
BmiI	GGNNCC	3 cut(s)	670, 734, 748
BmrFI	CCNGG	2 cut(s)	650, 1330
BmrI	ACTGGG	1 cut(s)	136
BmsI	GCATC	6 cut(s)	255, 399, 783, 870, 919, 1377
BmtI	GCTAGC	1 cut(s)	648
BmuI	ACTGGG	1 cut(s)	136
BpiI	GAAGAC	2 cut(s)	1081, 1271
BpmI	CTGGAG	1 cut(s)	733
BsaAI	YACGTR	2 cut(s)	370, 941
BsaJI	CCNNGG	5 cut(s)	120, 649, 1029, 1144, 1452
BsaWI	WCCGGW	1 cut(s)	252
BsaXI	ACNNNNNCTCC	2 cut(s)	893, 923
Bsc4I	CCNNNNNNNGG	7 cut(s)	67, 126, 230, 231, 681, 1150, 1329
Bse118I	RCCGGY	1 cut(s)	881
Bse1I	ACTGG	1 cut(s)	131
Bse3DI	GCAATG	1 cut(s)	1531
BseBI	CCWGG	2 cut(s)	650, 1330
BseDI	CCNNGG	5 cut(s)	120, 649, 1029, 1144, 1452
BseGI	GGATG	2 cut(s)	616, 934
BseLI	CCNNNNNNNGG	7 cut(s)	67, 126, 230, 231, 681, 1150, 1329
BseMI	GCAATG	1 cut(s)	1531
BseMII	CTCAG	6 cut(s)	18, 249, 564, 1032, 1199, 1262
BseNI	ACTGG	1 cut(s)	131
BseRI	GAGGAG	1 cut(s)	884
BseXI	GCAGC	4 cut(s)	63, 95, 318, 1166
BseYI	CCCAGC	1 cut(s)	1149
BsgI	GTGCAG	1 cut(s)	453
Bsh1236I	CGCG	2 cut(s)	88, 585
BshFI	GGCC	4 cut(s)	119, 145, 689, 1534
BsiHKAI	GWGCWC	2 cut(s)	1133, 1382
BsiSI	CCGG	3 cut(s)	61, 253, 882
BslFI	GGGAC	3 cut(s)	204, 401, 1129
BslI	CCNNNNNNNGG	7 cut(s)	67, 126, 230, 231, 681, 1150, 1329
BsmAI	GTCTC	1 cut(s)	1335
BsmFI	GGGAC	3 cut(s)	204, 401, 1129
BsnI	GGCC	4 cut(s)	119, 145, 689, 1534
Bsp1286I	GDGCHC	2 cut(s)	1133, 1382
Bsp143I	GATC	7 cut(s)	602, 668, 836, 849, 1188, 1439, 1489
Bsp19I	CCATGG	2 cut(s)	1029, 1144
BspACI	CCGC	5 cut(s)	16, 34, 235, 481, 768
BspANI	GGCC	4 cut(s)	119, 145, 689, 1534
BspCNI	CTCAG	6 cut(s)	19, 250, 565, 1033, 1198, 1263
BspFNI	CGCG	2 cut(s)	88, 585
BspHI	TCATGA	2 cut(s)	528, 864
BspLI	GGNNCC	3 cut(s)	670, 734, 748
BspOI	GCTAGC	1 cut(s)	648
BspPI	GGATC	3 cut(s)	663, 676, 831
BspQI	GCTCTTC	2 cut(s)	1071, 1208
BsrDI	GCAATG	1 cut(s)	1531
BsrFI	RCCGGY	1 cut(s)	881
BsrI	ACTGG	1 cut(s)	131
BssAI	RCCGGY	1 cut(s)	881
BssECI	CCNNGG	5 cut(s)	120, 649, 1029, 1144, 1452
BssMI	GATC	7 cut(s)	602, 668, 836, 849, 1188, 1439, 1489
BssT1I	CCWWGG	4 cut(s)	120, 1029, 1144, 1452
Bst2UI	CCWGG	2 cut(s)	650, 1330
Bst4CI	ACNGT	4 cut(s)	394, 968, 1177, 1386
Bst6I	CTCTTC	2 cut(s)	1071, 1208
BstBAI	YACGTR	2 cut(s)	370, 941
BstC8I	GCNNGC	6 cut(s)	58, 81, 481, 646, 883, 903
BstDEI	CTNAG	9 cut(s)	27, 258, 314, 573, 708, 1041, 1125, 1185, 1271
BstDSI	CCRYGG	2 cut(s)	1029, 1144
BstF5I	GGATG	2 cut(s)	616, 934
BstFNI	CGCG	2 cut(s)	88, 585
BstHHI	GCGC	3 cut(s)	88, 585, 1368
BstKTI	GATC	7 cut(s)	605, 671, 839, 852, 1191, 1442, 1492
BstMAI	GTCTC	1 cut(s)	1335
BstMBI	GATC	7 cut(s)	602, 668, 836, 849, 1188, 1439, 1489
BstMWI	GCNNNNNNNGC	1 cut(s)	85
BstNI	CCWGG	2 cut(s)	650, 1330
BstSCI	CCNGG	2 cut(s)	648, 1328
BstSFI	CTRYAG	1 cut(s)	474
BstUI	CGCG	2 cut(s)	88, 585
BstV1I	GCAGC	4 cut(s)	63, 95, 318, 1166
BstV2I	GAAGAC	2 cut(s)	1081, 1271
BstX2I	RGATCY	2 cut(s)	668, 1188
BstXI	CCANNNNNNTGG	2 cut(s)	309, 743
BstYI	RGATCY	2 cut(s)	668, 1188
BsuI	GTATCC	1 cut(s)	930
BsuRI	GGCC	4 cut(s)	119, 145, 689, 1534
BtgI	CCRYGG	2 cut(s)	1029, 1144
BtsCI	GGATG	2 cut(s)	616, 934
BtsI	GCAGTG	1 cut(s)	1512
BtsIMutI	CAGTG	1 cut(s)	1512
Cac8I	GCNNGC	6 cut(s)	58, 81, 481, 646, 883, 903
CciI	TCATGA	2 cut(s)	528, 864
CfoI	GCGC	3 cut(s)	88, 585, 1368
Cfr10I	RCCGGY	1 cut(s)	881
Cfr13I	GGNCC	3 cut(s)	117, 143, 687
CseI	GACGC	1 cut(s)	1276
DdeI	CTNAG	9 cut(s)	27, 258, 314, 573, 708, 1041, 1125, 1185, 1271
DpnI	GATC	7 cut(s)	604, 670, 838, 851, 1190, 1441, 1491
DpnII	GATC	7 cut(s)	602, 668, 836, 849, 1188, 1439, 1489
DrdI	GACNNNNNNGTC	1 cut(s)	554
DriI	GACNNNNNGTC	1 cut(s)	394
DseDI	GACNNNNNNGTC	1 cut(s)	554
EaeI	YGGCCR	1 cut(s)	1532
Eam1104I	CTCTTC	2 cut(s)	1071, 1208
Eam1105I	GACNNNNNGTC	1 cut(s)	394
EarI	CTCTTC	2 cut(s)	1071, 1208
Ecl136II	GAGCTC	2 cut(s)	1131, 1380
Eco130I	CCWWGG	4 cut(s)	120, 1029, 1144, 1452
Eco24I	GRGCYC	2 cut(s)	1133, 1382
Eco32I	GATATC	1 cut(s)	1277
Eco53kI	GAGCTC	2 cut(s)	1131, 1380
Eco72I	CACGTG	1 cut(s)	370
EcoICRI	GAGCTC	2 cut(s)	1131, 1380
EcoRII	CCWGG	2 cut(s)	648, 1328
EcoRV	GATATC	1 cut(s)	1277
EcoT14I	CCWWGG	4 cut(s)	120, 1029, 1144, 1452
EcoT22I	ATGCAT	2 cut(s)	742, 934
EcoT38I	GRGCYC	2 cut(s)	1133, 1382
ErhI	CCWWGG	4 cut(s)	120, 1029, 1144, 1452
FalI	AAGNNNNNCTT	2 cut(s)	792, 824
FaqI	GGGAC	3 cut(s)	204, 401, 1129
FauI	CCCGC	2 cut(s)	228, 488
FauNDI	CATATG	1 cut(s)	544
FblI	GTMKAC	1 cut(s)	1001
Fnu4HI	GCNGC	7 cut(s)	17, 35, 77, 84, 332, 768, 1155
FokI	GGATG	2 cut(s)	623, 941
FriOI	GRGCYC	2 cut(s)	1133, 1382
Fsp4HI	GCNGC	7 cut(s)	17, 35, 77, 84, 332, 768, 1155
FspBI	CTAG	4 cut(s)	194, 453, 645, 1557
GlaI	GCGC	3 cut(s)	87, 584, 1367
GluI	GCNGC	7 cut(s)	17, 35, 77, 84, 332, 768, 1155
GsaI	CCCAGC	1 cut(s)	1153
GsuI	CTGGAG	1 cut(s)	733
HaeIII	GGCC	4 cut(s)	119, 145, 689, 1534
HapII	CCGG	3 cut(s)	61, 253, 882
HgaI	GACGC	1 cut(s)	1276
HhaI	GCGC	3 cut(s)	88, 585, 1368
Hin6I	GCGC	3 cut(s)	86, 583, 1366
HinP1I	GCGC	3 cut(s)	86, 583, 1366
HincII	GTYRAC	1 cut(s)	324
HindII	GTYRAC	1 cut(s)	324
HindIII	AAGCTT	1 cut(s)	1306
HinfI	GANTC	5 cut(s)	260, 414, 945, 1232, 1343
HpaII	CCGG	3 cut(s)	61, 253, 882
HphI	GGTGA	4 cut(s)	418, 550, 1297, 1374
Hpy166II	GTNNAC	2 cut(s)	324, 1002
Hpy188III	TCNNGA	7 cut(s)	529, 750, 812, 847, 865, 1192, 1294
Hpy8I	GTNNAC	2 cut(s)	324, 1002
Hpy99I	CGWCG	2 cut(s)	1390, 1430
HpyAV	CCTTC	7 cut(s)	526, 617, 653, 659, 1035, 1276, 1427
HpyCH4III	ACNGT	4 cut(s)	394, 968, 1177, 1386
HpyCH4IV	ACGT	4 cut(s)	346, 369, 940, 998
HpyCH4V	TGCA	9 cut(s)	83, 153, 434, 450, 700, 740, 861, 932, 1505
HpyF10VI	GCNNNNNNNGC	1 cut(s)	85
HpyF3I	CTNAG	9 cut(s)	27, 258, 314, 573, 708, 1041, 1125, 1185, 1271
HpySE526I	ACGT	4 cut(s)	346, 369, 940, 998
HspAI	GCGC	3 cut(s)	86, 583, 1366
KroI	GCCGGC	1 cut(s)	881
KroNI	GCCGGC	1 cut(s)	883
Kzo9I	GATC	7 cut(s)	602, 668, 836, 849, 1188, 1439, 1489
LguI	GCTCTTC	2 cut(s)	1071, 1208
LmnI	GCTCC	4 cut(s)	53, 752, 910, 1136
Lsp1109I	GCAGC	4 cut(s)	63, 95, 318, 1166
LweI	GCATC	6 cut(s)	255, 399, 783, 870, 919, 1377
MaeI	CTAG	4 cut(s)	194, 453, 645, 1557
MaeII	ACGT	4 cut(s)	346, 369, 940, 998
MaeIII	GTNAC	4 cut(s)	347, 462, 556, 952
MalI	GATC	7 cut(s)	604, 670, 838, 851, 1190, 1441, 1491
MboI	GATC	7 cut(s)	602, 668, 836, 849, 1188, 1439, 1489
MboII	GAAGA	9 cut(s)	332, 409, 1006, 1042, 1058, 1081, 1225, 1276, 1449
MflI	RGATCY	2 cut(s)	668, 1188
MhlI	GDGCHC	2 cut(s)	1133, 1382
MlsI	TGGCCA	1 cut(s)	1534
MluCI	AATT	7 cut(s)	162, 169, 356, 496, 958, 1404, 1538
MluNI	TGGCCA	1 cut(s)	1534
MlyI	GAGTC	3 cut(s)	269, 1241, 1337
MmeI	TCCRAC	3 cut(s)	31, 378, 1408
Mox20I	TGGCCA	1 cut(s)	1534
Mph1103I	ATGCAT	2 cut(s)	742, 934
MroNI	GCCGGC	1 cut(s)	881
MscI	TGGCCA	1 cut(s)	1534
MseI	TTAA	3 cut(s)	209, 1095, 1407
MslI	CAYNNNNRTG	2 cut(s)	307, 1500
Msp20I	TGGCCA	1 cut(s)	1534
MspI	CCGG	3 cut(s)	61, 253, 882
MspR9I	CCNGG	2 cut(s)	650, 1330
MvaI	CCWGG	2 cut(s)	650, 1330
MvnI	CGCG	2 cut(s)	88, 585
MwoI	GCNNNNNNNGC	1 cut(s)	85
NaeI	GCCGGC	1 cut(s)	883
NcoI	CCATGG	2 cut(s)	1029, 1144
NdeI	CATATG	1 cut(s)	544
NdeII	GATC	7 cut(s)	602, 668, 836, 849, 1188, 1439, 1489
NgoMIV	GCCGGC	1 cut(s)	881
NheI	GCTAGC	1 cut(s)	644
NlaIV	GGNNCC	3 cut(s)	670, 734, 748
NmuCI	GTSAC	3 cut(s)	347, 556, 952
NsiI	ATGCAT	2 cut(s)	742, 934
PagI	TCATGA	2 cut(s)	528, 864
PciSI	GCTCTTC	2 cut(s)	1071, 1208
PcsI	WCGNNNNNNNCGW	1 cut(s)	1083
PdiI	GCCGGC	1 cut(s)	883
PfeI	GAWTC	2 cut(s)	414, 945
PfoI	TCCNGGA	1 cut(s)	1328
PkrI	GCNGC	7 cut(s)	18, 36, 78, 85, 333, 769, 1156
PleI	GAGTC	3 cut(s)	268, 1240, 1337
PmaCI	CACGTG	1 cut(s)	370
PmlI	CACGTG	1 cut(s)	370
PpsI	GAGTC	3 cut(s)	268, 1240, 1337
Ppu21I	YACGTR	2 cut(s)	370, 941
Psp124BI	GAGCTC	2 cut(s)	1133, 1382
Psp6I	CCWGG	2 cut(s)	648, 1328
PspCI	CACGTG	1 cut(s)	370
PspFI	CCCAGC	1 cut(s)	1149
PspGI	CCWGG	2 cut(s)	648, 1328
PspN4I	GGNNCC	3 cut(s)	670, 734, 748
PspPI	GGNCC	3 cut(s)	117, 143, 687
PsuI	RGATCY	2 cut(s)	668, 1188
RseI	CAYNNNNRTG	2 cut(s)	307, 1500
SacI	GAGCTC	2 cut(s)	1133, 1382
SapI	GCTCTTC	2 cut(s)	1071, 1208
SaqAI	TTAA	3 cut(s)	209, 1095, 1407
SatI	GCNGC	7 cut(s)	17, 35, 77, 84, 332, 768, 1155
Sau3AI	GATC	7 cut(s)	602, 668, 836, 849, 1188, 1439, 1489
Sau96I	GGNCC	3 cut(s)	117, 143, 687
SchI	GAGTC	3 cut(s)	269, 1241, 1337
ScrFI	CCNGG	2 cut(s)	650, 1330
SduI	GDGCHC	2 cut(s)	1133, 1382
SfaNI	GCATC	6 cut(s)	255, 399, 783, 870, 919, 1377
SfcI	CTRYAG	1 cut(s)	474
SmiMI	CAYNNNNRTG	2 cut(s)	307, 1500
Sse9I	AATT	7 cut(s)	162, 169, 356, 496, 958, 1404, 1538
SsiI	CCGC	5 cut(s)	16, 34, 235, 481, 768
SspMI	CTAG	4 cut(s)	194, 453, 645, 1557
SstI	GAGCTC	2 cut(s)	1133, 1382
StyD4I	CCNGG	2 cut(s)	648, 1328
StyI	CCWWGG	4 cut(s)	120, 1029, 1144, 1452
TaaI	ACNGT	4 cut(s)	394, 968, 1177, 1386
TaiI	ACGT	4 cut(s)	349, 372, 943, 1001
TaqI	TCGA	3 cut(s)	263, 601, 852
TasI	AATT	7 cut(s)	162, 169, 356, 496, 958, 1404, 1538
TauI	GCSGC	3 cut(s)	19, 37, 770
TfiI	GAWTC	2 cut(s)	414, 945
Tru1I	TTAA	3 cut(s)	209, 1095, 1407
Tru9I	TTAA	3 cut(s)	209, 1095, 1407
TscAI	CASTG	1 cut(s)	1512
TseFI	GTSAC	3 cut(s)	347, 556, 952
TseI	GCWGC	4 cut(s)	76, 83, 331, 1154
Tsp45I	GTSAC	3 cut(s)	347, 556, 952
TspDTI	ATGAA	6 cut(s)	517, 531, 545, 881, 1006, 1409
TspRI	CASTG	1 cut(s)	1512
XapI	RAATTY	1 cut(s)	958
XmiI	GTMKAC	1 cut(s)	1001
XspI	CTAG	4 cut(s)	194, 453, 645, 1557
Zsp2I	ATGCAT	2 cut(s)	742, 934

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.