Rroxscaffold_4G00311030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013752
Physical Location & Seq
Forward (+)
33256491 .. 33266703
10213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00311030.1

Sequence Viewer

Length: 537 bp
ATGGGGTGTGGCTGCTTGCAGCACTTGGCCCTTGTGGGGAGGCGGGTTTTGGACCGGGCCAATGTTCTTGGGCCCGTGCCATCTCTATGGCTCGGTCTGTTGGCCGTGCCGGCTATTTATCAAGGTGATGATGAATTACCCTCGCACGTGGTCCGGCGGTTTTGGGACACGGTGCTTGAGAGGGTGTTGCGATTCATCATCATATGGGTGGTGGTAGGGCTGTATCTTTACACTAGAGTTTGGCAGCTACCCATGGTAGCCCGCATGTATGGGCGAATTTGGTGGTTGGATGTTGGGCTTGTTTGGCTCACGAGGGTCGGTCTTTTTGGGCTGTTCCGCCACTATGGCTCTCGTTTGGAAGGAGTATTGTGGTTTGATACCACATATGGTTACTCCTATGTGGAAAACTTTATAGAAGCATGGAAACCAAAGGAAAATTCTCACTTTATATGTGGAGAGATTGATCACCAAAAGTGTCATACAATGATGATTCTTGCCTGTGAAATGGACCTGCACCCTCTTGATCATGTGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

20.77

Weight (kDa)

6.69

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 519
AciI CCGC 4 cut(s) 43, 157, 262, 337
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 2 cut(s) 276, 436
AcvI CACGTG 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 36
AjuI GAANNNNNNNTTGG 2 cut(s) 421, 453
AluBI AGCT 1 cut(s) 247
AluI AGCT 1 cut(s) 247
AoxI GGCC 4 cut(s) 27, 57, 71, 102
ApaI GGGCCC 1 cut(s) 75
ApeKI GCWGC 3 cut(s) 12, 19, 244
ApoI RAATTY 2 cut(s) 276, 436
AspS9I GGNCC 7 cut(s) 28, 52, 57, 71, 72, 151, 508
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 2 cut(s) 137, 458
AvaII GGWCC 3 cut(s) 52, 151, 508
BaeGI GKGCMC 1 cut(s) 75
BanII GRGCYC 1 cut(s) 75
BauI CACGAG 1 cut(s) 310
BbrPI CACGTG 1 cut(s) 148
BbvI GCAGC 2 cut(s) 31, 256
BccI CCATC 1 cut(s) 88
BceAI ACGGC 1 cut(s) 89
BclI TGATCA 2 cut(s) 463, 523
BcnI CCSGG 1 cut(s) 56
BfaI CTAG 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 519
BglI GCCNNNNNGGC 2 cut(s) 110, 345
BisI GCNGC 3 cut(s) 13, 20, 245
BlsI GCNGC 3 cut(s) 14, 21, 246
Bme1390I CCNGG 1 cut(s) 56
Bme18I GGWCC 3 cut(s) 52, 151, 508
BmgT120I GGNCC 7 cut(s) 28, 52, 57, 71, 72, 151, 508
BmiI GGNNCC 1 cut(s) 73
BmrFI CCNGG 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 197
BpuMI CCSGG 1 cut(s) 56
BsaAI YACGTR 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse118I RCCGGY 1 cut(s) 109
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 295
BseLI CCNNNNNNNGG 1 cut(s) 36
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 2 cut(s) 31, 256
BsgI GTGCAG 1 cut(s) 497
BshFI GGCC 4 cut(s) 29, 59, 73, 104
BsiSI CCGG 3 cut(s) 55, 110, 154
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 36
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 4 cut(s) 29, 59, 73, 104
Bsp120I GGGCCC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 463, 523
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 4 cut(s) 43, 157, 262, 337
BspANI GGCC 4 cut(s) 29, 59, 73, 104
BspLI GGNNCC 1 cut(s) 73
BspMI ACCTGC 1 cut(s) 519
BsrFI RCCGGY 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 2 cut(s) 463, 523
BssSI CACGAG 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 310
Bst4CI ACNGT 1 cut(s) 172
BstBAI YACGTR 1 cut(s) 148
BstC8I GCNNGC 3 cut(s) 17, 111, 262
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 295
BstKTI GATC 2 cut(s) 466, 526
BstMBI GATC 2 cut(s) 463, 523
BstMWI GCNNNNNNNGC 3 cut(s) 110, 304, 345
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 54
BstSLI GKGCMC 1 cut(s) 75
BstV1I GCAGC 2 cut(s) 31, 256
BstXI CCANNNNNNTGG 1 cut(s) 87
BsuRI GGCC 4 cut(s) 29, 59, 73, 104
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 295
BveI ACCTGC 1 cut(s) 519
Cac8I GCNNGC 3 cut(s) 17, 111, 262
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 7 cut(s) 28, 52, 57, 71, 72, 151, 508
CviAII CATG 4 cut(s) 253, 265, 420, 527
DpnI GATC 2 cut(s) 465, 525
DpnII GATC 2 cut(s) 463, 523
EaeI YGGCCR 1 cut(s) 102
EciI GGCGGA 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 75
Eco47I GGWCC 3 cut(s) 52, 151, 508
Eco72I CACGTG 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 4 cut(s) 256, 268, 423, 530
FaqI GGGAC 1 cut(s) 179
FatI CATG 4 cut(s) 252, 264, 419, 526
FauI CCCGC 2 cut(s) 36, 269
FauNDI CATATG 2 cut(s) 203, 385
FbaI TGATCA 2 cut(s) 463, 523
Fnu4HI GCNGC 3 cut(s) 13, 20, 245
FokI GGATG 1 cut(s) 302
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 3 cut(s) 13, 20, 245
FspBI CTAG 1 cut(s) 234
GluI GCNGC 3 cut(s) 13, 20, 245
HaeIII GGCC 4 cut(s) 29, 59, 73, 104
HapII CCGG 3 cut(s) 55, 110, 154
Hin1II CATG 4 cut(s) 256, 268, 423, 530
HinfI GANTC 2 cut(s) 192, 490
HpaII CCGG 3 cut(s) 55, 110, 154
HphI GGTGA 2 cut(s) 137, 458
Hpy188III TCNNGA 2 cut(s) 310, 521
HpyAV CCTTC 1 cut(s) 353
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 2 cut(s) 19, 514
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 304, 345
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 4 cut(s) 256, 268, 423, 530
KroI GCCGGC 1 cut(s) 109
KroNI GCCGGC 1 cut(s) 111
Ksp22I TGATCA 2 cut(s) 463, 523
Kzo9I GATC 2 cut(s) 463, 523
LpnPI CCDG 5 cut(s) 68, 123, 167, 511, 524
Lsp1109I GCAGC 2 cut(s) 31, 256
MaeI CTAG 1 cut(s) 234
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 465, 525
MboI GATC 2 cut(s) 463, 523
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 3 cut(s) 134, 276, 436
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 5 cut(s) 33, 151, 174, 306, 528
MroNI GCCGGC 1 cut(s) 109
MslI CAYNNNNRTG 2 cut(s) 85, 206
MspI CCGG 3 cut(s) 55, 110, 154
MspR9I CCNGG 1 cut(s) 56
MwoI GCNNNNNNNGC 3 cut(s) 110, 304, 345
NaeI GCCGGC 1 cut(s) 111
NciI CCSGG 1 cut(s) 56
NcoI CCATGG 1 cut(s) 252
NdeI CATATG 2 cut(s) 203, 385
NdeII GATC 2 cut(s) 463, 523
NgoMIV GCCGGC 1 cut(s) 109
NlaIII CATG 4 cut(s) 256, 268, 423, 530
NlaIV GGNNCC 1 cut(s) 73
NspI RCATGY 1 cut(s) 268
PdiI GCCGGC 1 cut(s) 111
PfeI GAWTC 2 cut(s) 192, 490
PkrI GCNGC 3 cut(s) 14, 21, 246
PmaCI CACGTG 1 cut(s) 148
PmlI CACGTG 1 cut(s) 148
Ppu21I YACGTR 1 cut(s) 148
PspCI CACGTG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 73
PspOMI GGGCCC 1 cut(s) 71
PspPI GGNCC 7 cut(s) 28, 52, 57, 71, 72, 151, 508
RseI CAYNNNNRTG 2 cut(s) 85, 206
SatI GCNGC 3 cut(s) 13, 20, 245
Sau3AI GATC 2 cut(s) 463, 523
Sau96I GGNCC 7 cut(s) 28, 52, 57, 71, 72, 151, 508
ScrFI CCNGG 1 cut(s) 56
SduI GDGCHC 1 cut(s) 75
SetI ASST 4 cut(s) 127, 150, 249, 513
SinI GGWCC 3 cut(s) 52, 151, 508
SmiMI CAYNNNNRTG 2 cut(s) 85, 206
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 3 cut(s) 134, 276, 436
SsiI CCGC 4 cut(s) 43, 157, 262, 337
SspMI CTAG 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 54
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 150
TaqII GACCGA 2 cut(s) 83, 308
TasI AATT 3 cut(s) 134, 276, 436
TfiI GAWTC 2 cut(s) 192, 490
TseI GCWGC 3 cut(s) 12, 19, 244
TspDTI ATGAA 2 cut(s) 147, 184
VpaK11BI GGWCC 3 cut(s) 52, 151, 508
XapI RAATTY 2 cut(s) 276, 436
XceI RCATGY 1 cut(s) 268
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.