Rroxscaffold_160G00433970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000160
OG0013752
Physical Location & Seq
Forward (+)
479949 .. 483352
3404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_160G00433970.1

Sequence Viewer

Length: 423 bp
ATGAGCGTTTTTACTAAAGAAATTCACCAGGATATGCAACGTATTATGAGTCGGTTTCATGACTTTGTCAGTAGCCTGATAAAAGAGGGGAAAGGAGGAACGCGCCTCACGCGCCTAGGTAAAAAGGAAAAAATCCGAGGAGTGTGTTCCTCTCCCCGCCGAACACGGCGAGCGATCTCGCCGCCGCGTTTCGGTCCGGGAGAGGTTGATGGTTCGGTGGTGGAGTGGGCTCTCGGGGCGGCGGTGTGGTCCGGGGAGAGTGGAGGTTTCTCCGTTTTCGGGTTGACTTCGGCGCGGCGGCTTGTTGTTGTGTGGCGGCGGTTTGATGGCGATGGGGCTCGTCGACAATGGTTGTGGCCGCTTGCGCACTTGGCCTTTGTGGGGGAGACGGGTTTTGGACCGGGCCAATGTTCTTGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

15.55

Weight (kDa)

11.03

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 366
AccI GTMKAC 1 cut(s) 343
AccII CGCG 4 cut(s) 103, 112, 187, 295
AcoI YGGCCR 1 cut(s) 356
AcsI RAATTY 1 cut(s) 21
AfiI CCNNNNNNNGG 3 cut(s) 191, 279, 381
AjnI CCWGG 1 cut(s) 27
Alw26I GTCTC 1 cut(s) 380
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 4 cut(s) 356, 372, 403, 417
ApoI RAATTY 1 cut(s) 21
AspA2I CCTAGG 1 cut(s) 115
AspLEI GCGC 4 cut(s) 105, 114, 295, 367
AspS9I GGNCC 5 cut(s) 194, 249, 398, 403, 417
AsuC2I CCSGG 3 cut(s) 198, 253, 402
AsuHPI GGTGA 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 3 cut(s) 194, 249, 398
AvrII CCTAGG 1 cut(s) 115
BanII GRGCYC 2 cut(s) 232, 340
BccI CCATC 3 cut(s) 203, 320, 326
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 29
BcnI CCSGG 3 cut(s) 198, 253, 402
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 2 cut(s) 116, 421
BisI GCNGC 7 cut(s) 182, 185, 240, 296, 299, 317, 359
BlnI CCTAGG 1 cut(s) 115
BlsI GCNGC 7 cut(s) 183, 186, 241, 297, 300, 318, 360
Bme1390I CCNGG 4 cut(s) 29, 198, 253, 402
Bme18I GGWCC 3 cut(s) 194, 249, 398
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 5 cut(s) 194, 249, 398, 403, 417
BmrFI CCNGG 4 cut(s) 29, 198, 253, 402
BpuMI CCSGG 3 cut(s) 198, 253, 402
BsaJI CCNNGG 3 cut(s) 115, 136, 252
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 279, 381
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 3 cut(s) 115, 136, 252
BseLI CCNNNNNNNGG 3 cut(s) 191, 279, 381
BseRI GAGGAG 1 cut(s) 153
Bsh1236I CGCG 4 cut(s) 103, 112, 187, 295
BshFI GGCC 4 cut(s) 358, 374, 405, 419
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 3 cut(s) 197, 252, 401
BslI CCNNNNNNNGG 3 cut(s) 191, 279, 381
BsmAI GTCTC 1 cut(s) 380
BsmBI CGTCTC 1 cut(s) 380
BsnI GGCC 4 cut(s) 358, 374, 405, 419
BsoBI CYCGRG 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 232, 340
Bsp143I GATC 1 cut(s) 174
BspANI GGCC 4 cut(s) 358, 374, 405, 419
BspFNI CGCG 4 cut(s) 103, 112, 187, 295
BspHI TCATGA 1 cut(s) 58
BssECI CCNNGG 3 cut(s) 115, 136, 252
BssMI GATC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 29
BstC8I GCNNGC 2 cut(s) 171, 363
BstFNI CGCG 4 cut(s) 103, 112, 187, 295
BstHHI GCGC 4 cut(s) 105, 114, 295, 367
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 5 cut(s) 109, 111, 236, 364, 371
BstNI CCWGG 1 cut(s) 29
BstSCI CCNGG 4 cut(s) 27, 196, 251, 400
BstUI CGCG 4 cut(s) 103, 112, 187, 295
BsuRI GGCC 4 cut(s) 358, 374, 405, 419
BtgZI GCGATG 1 cut(s) 345
Cac8I GCNNGC 2 cut(s) 171, 363
CciI TCATGA 1 cut(s) 58
CfoI GCGC 4 cut(s) 105, 114, 295, 367
Cfr13I GGNCC 5 cut(s) 194, 249, 398, 403, 417
CpoI CGGWCCG 1 cut(s) 194
CspCI CAANNNNNGTGG 2 cut(s) 335, 370
CspI CGGWCCG 1 cut(s) 194
CviAII CATG 1 cut(s) 59
CviJI RGCY 8 cut(s) 75, 230, 301, 338, 358, 374, 405, 419
CviKI_1 RGCY 8 cut(s) 75, 230, 301, 338, 358, 374, 405, 419
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 356
Eco130I CCWWGG 1 cut(s) 115
Eco24I GRGCYC 2 cut(s) 232, 340
Eco47I GGWCC 3 cut(s) 194, 249, 398
Eco88I CYCGRG 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 115
EcoT38I GRGCYC 2 cut(s) 232, 340
ErhI CCWWGG 1 cut(s) 115
Esp3I CGTCTC 1 cut(s) 380
FaeI CATG 1 cut(s) 62
FaiI YATR 3 cut(s) 35, 47, 60
FatI CATG 1 cut(s) 58
FauI CCCGC 1 cut(s) 164
FblI GTMKAC 1 cut(s) 343
Fnu4HI GCNGC 7 cut(s) 182, 185, 240, 296, 299, 317, 359
FriOI GRGCYC 2 cut(s) 232, 340
Fsp4HI GCNGC 7 cut(s) 182, 185, 240, 296, 299, 317, 359
FspBI CTAG 2 cut(s) 116, 421
FspI TGCGCA 1 cut(s) 366
GlaI GCGC 4 cut(s) 104, 113, 294, 366
GluI GCNGC 7 cut(s) 182, 185, 240, 296, 299, 317, 359
HaeIII GGCC 4 cut(s) 358, 374, 405, 419
HapII CCGG 3 cut(s) 197, 252, 401
HhaI GCGC 4 cut(s) 105, 114, 295, 367
Hin1II CATG 1 cut(s) 62
Hin6I GCGC 4 cut(s) 103, 112, 293, 365
HinP1I GCGC 4 cut(s) 103, 112, 293, 365
HincII GTYRAC 2 cut(s) 285, 344
HindII GTYRAC 2 cut(s) 285, 344
HinfI GANTC 1 cut(s) 49
HpaII CCGG 3 cut(s) 197, 252, 401
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 2 cut(s) 285, 344
Hpy188I TCNGA 1 cut(s) 137
Hpy188III TCNNGA 1 cut(s) 59
Hpy8I GTNNAC 2 cut(s) 285, 344
Hpy99I CGWCG 1 cut(s) 345
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 5 cut(s) 109, 111, 236, 364, 371
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 1 cut(s) 62
HspAI GCGC 4 cut(s) 103, 112, 293, 365
Kzo9I GATC 1 cut(s) 174
LpnPI CCDG 6 cut(s) 14, 41, 89, 210, 265, 414
MaeI CTAG 2 cut(s) 116, 421
MaeII ACGT 1 cut(s) 40
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MhlI GDGCHC 2 cut(s) 232, 340
MluCI AATT 1 cut(s) 21
MlyI GAGTC 1 cut(s) 58
MnlI CCTC 7 cut(s) 79, 89, 116, 131, 160, 196, 257
MspI CCGG 3 cut(s) 197, 252, 401
MspR9I CCNGG 4 cut(s) 29, 198, 253, 402
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 4 cut(s) 103, 112, 187, 295
MwoI GCNNNNNNNGC 5 cut(s) 109, 111, 236, 364, 371
NciI CCSGG 3 cut(s) 198, 253, 402
NdeII GATC 1 cut(s) 174
NlaIII CATG 1 cut(s) 62
NsbI TGCGCA 1 cut(s) 366
PagI TCATGA 1 cut(s) 58
PflFI GACNNNGTC 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 196
PkrI GCNGC 7 cut(s) 183, 186, 241, 297, 300, 318, 360
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspPI GGNCC 5 cut(s) 194, 249, 398, 403, 417
PsyI GACNNNGTC 1 cut(s) 65
Rsr2I CGGWCCG 1 cut(s) 194
RsrII CGGWCCG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 342
SatI GCNGC 7 cut(s) 182, 185, 240, 296, 299, 317, 359
Sau3AI GATC 1 cut(s) 174
Sau96I GGNCC 5 cut(s) 194, 249, 398, 403, 417
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 4 cut(s) 29, 198, 253, 402
SduI GDGCHC 2 cut(s) 232, 340
SetI ASST 4 cut(s) 43, 121, 207, 268
SinI GGWCC 3 cut(s) 194, 249, 398
Sse9I AATT 1 cut(s) 21
SspMI CTAG 2 cut(s) 116, 421
StyD4I CCNGG 4 cut(s) 27, 196, 251, 400
StyI CCWWGG 1 cut(s) 115
TaiI ACGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 343
TaqII GACCGA 1 cut(s) 182
TasI AATT 1 cut(s) 21
TauI GCSGC 7 cut(s) 184, 187, 242, 298, 301, 319, 361
TspDTI ATGAA 1 cut(s) 47
TspGWI ACGGA 1 cut(s) 262
Tth111I GACNNNGTC 1 cut(s) 65
VpaK11BI GGWCC 3 cut(s) 194, 249, 398
XapI RAATTY 1 cut(s) 21
XmaJI CCTAGG 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 343
XspI CTAG 2 cut(s) 116, 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.